ViewGene
Locus Name:
GeneID:
Location:

GeneID:1833
TrivialName: PA14_25980
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25980
PAO1 Ortholog Locus:PA2943
Sequence Length:1095
Protein Length:364
Start: 2269871
Stop: 2268777
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06331.1|AE004720_7 (AE004720) phospho-2-dehydro-3-deoxyheptonate aldolase [Pseudomonas aeruginosa PAO1]
Identities = 360/364 (98%), Positives = 360/364 (98%)
Sequence:
ATGAACTCATCCGTATCCGCACTGACCGCCCTTTCGCCGAACGCCCAGGCCGTCGCCCTGCCCAGCGCCACC
GCCCGTACCGCCCTGGAACGCCTGCCCAGCCCGCTCGAACTCAAGGCTCGCCAGCCGTTGCCGCTGGAGCTG
GCCAGCCAGGTCGCCGCCAGCCGCGACGCCATCCGCGCCATCCTCGACGGCGACGACCCACGCCTGCTGGTG
GTAGTCGGTCCCTGTTCGATCCACGATCCCCGCTCCGCCCTCGAATACGCCGAACGCCTCGCCGCGCTGAGC
GACGAACTCGACGGCCAGTTGCTCCTGGTGATGCGCGCCTACGTGGAAAAGCCACGCACCACGGTGGGCTGG
AAGGGCCTCGCCTACGACCCGCACCTGGATGGCAGCGACGACATGGCCGCCGGCCTGGCGCTATCCCGGCAA
CTGATGCTGGACATGCTGCGCCTCGGCCTGCCGCTGGCCACCGAACTCCTGCAACCGCTGGCCGCCGGCTAC
TTCGACGACCTGCTGAGCTGGGCGGCGATCGGCGCGCGCACCAGCGAATCGCAGATCCACCGCGAGCTGGTC
AGCGGCCTCGGCCTGCCGGTGGGCTTCAAGAACGGCACCGACGGCGGCGTCGGCATCGCCTGCGACGCGATC
CGTTCGGCGGCCCACGGCCACCGCCATTTCGGCCTCGACCCGCACGGTCACCCGGCGCTGATCGAGACCCGC
GGCAATCCGGACACCCATGTGGTGCTGCGCGGCGGCCGCCACGGTTCGAACTACGGTCCCGAGCAGGTGCAG
GCCGTACGCCAGGCCCTGGAAAGCCAGGGCATCGCCACGCGGATGATGGTCGATTGCAGTCACGCCAACAGC
GGCAAGAACCCGCTGAACCAGCCCGGCGTCCTCGCCAGCGTGCTGGACCAGCGCCTGGTCGGCGCCAGCGAC
CTGGTCGGGGTAATGCTGGAAAGCCACCTGTTCGACGGTTGCCAGGCGCTCGGCGGCGAACTGCGCTACGGC
GTCTCGATCACCGACGGCTGCCTCGGCTGGGAAGGCACCGAGCGGATTCTCCGCGACGCCGCCCGGCGGCTC
GCCGAGGGGCGCTGA
Translation:
MNSSVSALTALSPNAQAVALPSATARTALERLPSPLELKARQPLPLELASQVAASRDAIRAILDGDDPRLLV
VVGPCSIHDPRSALEYAERLAALSDELDGQLLLVMRAYVEKPRTTVGWKGLAYDPHLDGSDDMAAGLALSRQ
LMLDMLRLGLPLATELLQPLAAGYFDDLLSWAAIGARTSESQIHRELVSGLGLPVGFKNGTDGGVGIACDAI
RSAAHGHRHFGLDPHGHPALIETRGNPDTHVVLRGGRHGSNYGPEQVQAVRQALESQGIATRMMVDCSHANS
GKNPLNQPGVLASVLDQRLVGASDLVGVMLESHLFDGCQALGGELRYGVSITDGCLGWEGTERILRDAARRL
AEGR*
AnnotationID:1842GeneID:1833
AnnotatorUID: diggins
Modification Date/Time: 2005-04-08 13:01:17
Gene Name:aroF
Confidence Code:2
GeneProduct:phospho-2-dehydro-3-deoxyheptonate aldolase
Cell Localization:()
Synonyms:aroG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthas
Functional Category Confidence Code:5
COGs:COG0722
Secondary Functional Category(ies):
EC Number:4.1.2.15
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68688.1|AE016498_1 (AE016785) phospho-2-dehydro-3-deoxyheptonate aldolase, class I
           [Pseudomonas putida KT2440]
          Length = 353

 Score =  496 bits (1277), Expect = e-140
 Identities = 245/341 (71%), Positives = 277/341 (81%), Gaps = 1/341 (0%)

>gb|AAO56517.1| (AE016866) phospho-2-dehydro-3-deoxyheptonate aldolase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 360

 Score =  489 bits (1260), Expect = e-138
 Identities = 246/360 (68%), Positives = 288/360 (80%), Gaps = 3/360 (0%)

>gb|AAG57712.1|AE005490_7 (AE005490) 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase
           (DAHP synthetase, tyrosine repressible) [Escherichia
           coli O157:H7 EDL933]
          Length = 356

 Score =  345 bits (885), Expect = 2e-94
 Identities = 172/327 (52%), Positives = 228/327 (69%), Gaps = 4/327 (1%)








 
Structural Features:
COG0722, AroG, 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase 
[Amino acid transport and metabolism]






   
Genomic Context:






   
Comment:


















    ReferenceID:3669




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20816




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36096




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1833_cdd.html


    		




 
URL:

















    ReferenceID:36422




  
Author/Investigator(s):
        Xie G, Bonner CA, Jensen RA.





  
Title:
        A probable mixed-function supraoperon in Pseudomonas exhibits gene organization features of both intergenomic conservation and gene shuffling.




  
PubMed:
        10368439


MedLine:
        




    
Source:
J Mol Evol. 1999 Jul;49(1):108-21.

Erratum in:
    * J Mol Evol 2000 Feb;50(2):202.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10368439

















    ReferenceID:36423




  
Author/Investigator(s):
        Hudson GS, Davidson BE.





  
Title:
        Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12.




  
PubMed:
        6396419


MedLine:
        




    
Source:
J Mol Biol. 1984 Dec 25;180(4):1023-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6396419

















    ReferenceID:36424




  
Author/Investigator(s):
        Shultz J, Hermodson MA, Garner CC, Herrmann KM.





  
Title:
        The nucleotide sequence of the aroF gene of Escherichia coli and the amino acid sequence of the encoded protein, the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase.




  
PubMed:
        6146618


MedLine:
        




    
Source:
 J Biol Chem. 1984 Aug 10;259(15):9655-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6146618









           
Homologs By Global Alignment 

           

       



     
Gene ID:1833
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3216
                           PA2943_tr
                           translation of PA2943
                           364
                           98.90
                           98.90
                           0
                           1842.0
     

     
     
99698
                           gb|AAG06331.1|AE004720_7
                           (AE004720) phospho-2-dehydro-3-deoxyheptonate aldolase [Pseudomonas aeruginosa PAO1]
                           364
                           98.90
                           98.90
                           0
                           1842.0
     

     
     
99700
                           gb|AAO56517.1|
                           (AE016866) phospho-2-dehydro-3-deoxyheptonate aldolase [Pseudomonas syringae pv. tomato str. DC3000]
                           364
                           67.58
                           79.12
                           4
                           1259.0
     

     
     
99699
                           gb|AAN68688.1|AE016498_1
                           (AE016785) phospho-2-dehydro-3-deoxyheptonate aldolase, class I [Pseudomonas putida KT2440]
                           369
                           67.20
                           76.69
                           21
                           1280.0
     

     
     
99724
                           emb|CAE22422.1|
                           (BX572101) DAHP synthetase class I [Prochlorococcus marinus str. MIT 9313]
                           372
                           47.84
                           60.21
                           20
                           820.0
     

     






      

          
GC ORFID: 44835How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2943Glimmer Score: 

          
Start: 2684054Stop: 2685148

          
Length: 1095


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2943 translation of PA2943Homolog Bit Score 714.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6041 (ORF: BLASTX 499.0), psyr_15may02_Scaffold13_revised_gene2184 (ORF: BLASTX 328.0), t_PA2943 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACTCATCCGTATCCGCACTGACCGCCCTTTCGCCGAACGCCCAGGCCGTCGCCCTGCCCAGCGCCACC 
GCCCGTACCGCCCTGGAACGCCTGCCCAGCCCGCTCGAACTCAAGGCTCGCCAGCCGTTGCCGCTGGAGCTG 
GCCAGCCAGGTCGCCGCCAGCCGCGACGCCATCCGCGCCATCCTCGACGGCGACGACCCACGCCTGCTGGTG 
GTAGTCGGTCCCTGTTCGATCCACGATCCCCGCTCCGCCCTCGAATACGCCGAACGCCTCGCCGCGCTGAGC 
GACGAACTCGACGGCCAGTTGCTCCTGGTGATGCGCGCCTACGTGGAAAAGCCACGCACCACGGTGGGCTGG 
AAGGGCCTCGCCTACGACCCGCACCTGGATGGCAGCGACGACATGGCCGCCGGCCTGGCGCTATCCCGGCAA 
CTGATGCTGGACATGCTGCGCCTCGGCCTGCCGCTGGCCACCGAACTCCTGCAACCGCTGGCCGCCGGCTAC 
TTCGACGACCTGCTGAGCTGGGCGGCGATCGGCGCGCGCACCAGCGAATCGCAGATCCACCGCGAGCTGGTC 
AGCGGCCTCGGCCTGCCGGTGGGCTTCAAGAACGGCACCGACGGCGGCGTCGGCATCGCCTGCGACGCGATC 
CGTTCGGCGGCCCACGGCCACCGCCATTTCGGCCTCGACCCGCACGGTCACCCGGCGCTGATCGAGACCCGC 
GGCAATCCGGACACCCATGTGGTGCTGCGCGGCGGCCGCCACGGTTCGAACTACGGTCCCGAGCAGGTGCAG 
GCCGTACGCCAGGCCCTGGAAAGCCAGGGCATCGCCACGCGGATGATGGTCGATTGCAGTCACGCCAACAGC 
GGCAAGAACCCGCTGAACCAGCCCGGCGTCCTCGCCAGCGTGCTGGACCAGCGCCTGGTCGGCGCCAGCGAC 
CTGGTCGGGGTAATGCTGGAAAGCCACCTGTTCGACGGTTGCCAGGCGCTCGGCGGCGAACTGCGCTACGGC 
GTCTCGATCACCGACGGCTGCCTCGGCTGGGAAGGCACCGAGCGGATTCTCCGCGACGCCGCCCGGCGGCTC 
GCCGAGGGGCGCTGA