ViewGene
Locus Name:
GeneID:
Location:

GeneID:1823
TrivialName: PA14_20560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20560
PAO1 Ortholog Locus:PA3366
Sequence Length:1041
Protein Length:346
Start: 1768608
Stop: 1769648
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06754.1|AE004759_1 (AE004759) aliphatic amidase [Pseudomonas aeruginosa PAO1]
Identities = 345/346 (99%), Positives = 346/346 (100%)
Sequence:
ATGCGTCACGGCGATATTTCCAGCAGCAACGACACCGTCGGAGTAGCGGTGGTCAACTACAAGATGCCGCGC
CTGCACACCGCGGCGGAGGTCCTGGACAACGCCCGGAAGATCGCCGACATGATCGTCGGCATGAAGCAGGGC
CTACCCGGCATGGACCTGGTGGTGTTCCCCGAGTACAGCCTGCAGGGCATCATGTACGATCCGGCGGAGATG
ATGGAAACCGCGGTGGCGATCCCCGGCGAGGAAACCGAGATATTCTCCCGCGCCTGCCGCAAGGCCAACGTC
TGGGGCGTATTCTCCCTCACCGGCGAACGGCACGAGGAGCATCCGCGCAAGGCGCCGTACAACACCCTGGTG
CTGATCGACAACAACGGCGAGATCGTCCAGAAGTACCGCAAGATCATTCCCTGGTGCCCCATCGAGGGCTGG
TATCCCGGTGGCCAGACCTACGTCAGCGAAGGGCCGAAGGGCATGAAGATCAGCCTGATCATCTGCGACGAC
GGCAACTACCCGGAAATCTGGCGCGACTGCGCGATGAAGGGCGCCGAGCTGATCGTGCGCTGCCAGGGCTAC
ATGTACCCGGCCAAGGACCAGCAGGTGATGATGGCCAAGGCCATGGCCTGGGCCAACAACTGCTATGTGGCG
GTGGCCAACGCCGCCGGCTTCGACGGCGTGTATTCCTACTTCGGCCACTCGGCGATCATCGGCTTCGACGGT
CGTACCCTGGGTGAGTGCGGCGAGGAGGAAATGGGTATCCAGTACGCCCAGCTGTCGCTCTCGCAGATCCGC
GATGCGCGCGCCAACGATCAGTCGCAGAACCACCTGTTCAAGATCCTCCACCGCGGCTACAGCGGCTTGCAG
GCATCCGGCGATGGCGACCGGGGCCTGGCGGAATGTCCGTTCGAGTTCTACCGCACCTGGGTCACCGACGCC
GAGAAGGCGCGCGAGAATGTCGAGCGTCTGACCCGCTCGACTACCGGCGTGGCGCAATGCCCGGTCGGCCGG
CTGCCCTACGAGGGACTGGAGAAGGAGGCCTGA
Translation:
MRHGDISSSNDTVGVAVVNYKMPRLHTAAEVLDNARKIADMIVGMKQGLPGMDLVVFPEYSLQGIMYDPAEM
METAVAIPGEETEIFSRACRKANVWGVFSLTGERHEEHPRKAPYNTLVLIDNNGEIVQKYRKIIPWCPIEGW
YPGGQTYVSEGPKGMKISLIICDDGNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVMMAKAMAWANNCYVA
VANAAGFDGVYSYFGHSAIIGFDGRTLGECGEEEMGIQYAQLSLSQIRDARANDQSQNHLFKILHRGYSGLQ
ASGDGDRGLAECPFEFYRTWVTDAEKARENVERLTRSTTGVAQCPVGRLPYEGLEKEA*
AnnotationID:1832GeneID:1823
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 07:07:33
Gene Name:amiE
Confidence Code:1
GeneProduct:aliphatic amidase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00795,COG0388
Secondary Functional Category(ies):
EC Number:3.5.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAF14257.1| (AF136599) aliphatic amidase [Geobacillus stearothermophilus]
          Length = 348

 Score =  610 bits (1573), Expect = e-174
 Identities = 284/346 (82%), Positives = 313/346 (90%)

>gb|AAA26186.1| (M88614) aliphatic amidase [Rhodococcus erythropolis]
          Length = 345

 Score =  600 bits (1546), Expect = e-171
 Identities = 278/340 (81%), Positives = 307/340 (90%)

>gb|AAD07363.1| (AE000548) aliphatic amidase (aimE) [Helicobacter pylori 26695]
          Length = 339

 Score =  558 bits (1438), Expect = e-158
 Identities = 257/338 (76%), Positives = 297/338 (87%), Gaps = 1/338 (0%)








 
Structural Features:
pfam00795, CN_hydrolase, Carbon-nitrogen hydrolase
CD-Length = 174 residues,  99.4% aligned
Score =  126 bits (318), Expect = 3e-30
Identities = 59/185 (31%), Positives = 84/185 (45%), Gaps = 20/185 (10%)

COG0388, Predicted amidohydrolase
CD-Length = 274 residues,  91.6% aligned
Score =  149 bits (378), Expect = 4e-37
Identities = 88/263 (33%), Positives = 118/263 (44%), Gaps = 20/263 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3649




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20796




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32192




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1823_cdd.html


    		




 
URL:

















    ReferenceID:33992




  
Author/Investigator(s):
        Brammar WJ, Charles IG, Matfield M, Liu CP, Drew RE, Clarke PH.





  
Title:
        The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa.




  
PubMed:
        3108030


MedLine:
        




    
Source:
FEBS Lett. 1987 May 11;215(2):291-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3108030

















    ReferenceID:33993




  
Author/Investigator(s):
        Ambler RP, Auffret AD, Clarke PH.





  
Title:
        The amino acid sequence of the aliphatic amidase from Pseudomonas aeruginosa.




  
PubMed:
        3108029


MedLine:
        




    
Source:
FEBS Lett. 1987 May 11;215(2):285-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3108029

















    ReferenceID:33994




  
Author/Investigator(s):
        Wilson SA, Drew RE.





  
Title:
        Transcriptional analysis of the amidase operon from Pseudomonas aeruginosa.




  
PubMed:
        7539417


MedLine:
        




    
Source:
J Bacteriol. 1995 Jun;177(11):3052-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7539417









           
Homologs By Global Alignment 

           

       



     
Gene ID:1823
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3204
                           PA3366_tr
                           translation of PA3366
                           346
                           99.71
                           99.99
                           0
                           1848.0
     

     
     
99533
                           gb|AAG06754.1|AE004759_1
                           (AE004759) aliphatic amidase [Pseudomonas aeruginosa PAO1]
                           346
                           99.71
                           99.99
                           0
                           1848.0
     

     
     
99534
                           gb|AAA25697.1|
                           (M27612) aliphatic amidase [Pseudomonas aeruginosa]
                           346
                           99.42
                           99.71
                           0
                           1841.0
     

     
     
99535
                           gb|AAF14257.1|
                           (AF136599) aliphatic amidase [Geobacillus stearothermophilus]
                           348
                           81.60
                           89.94
                           2
                           1573.0
     

     
     
99536
                           gb|AAO23013.1|
                           (AY184492) amidase [Bacillus sp. RAPc8]
                           348
                           81.03
                           89.94
                           2
                           1566.0
     

     






      

          
GC ORFID: 44825How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3366Glimmer Score: 

          
Start: 3185324Stop: 3184284

          
Length: 1041


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3366 translation of PA3366Homolog Bit Score 716.0

          
Other Homologs:  t_PA3366 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTCACGGCGATATTTCCAGCAGCAACGACACCGTCGGAGTAGCGGTGGTCAACTACAAGATGCCGCGC 
CTGCACACCGCGGCGGAGGTCCTGGACAACGCCCGGAAGATCGCCGACATGATCGTCGGCATGAAGCAGGGC 
CTACCCGGCATGGACCTGGTGGTGTTCCCCGAGTACAGCCTGCAGGGCATCATGTACGATCCGGCGGAGATG 
ATGGAAACCGCGGTGGCGATCCCCGGCGAGGAAACCGAGATATTCTCCCGCGCCTGCCGCAAGGCCAACGTC 
TGGGGCGTATTCTCCCTCACCGGCGAACGGCACGAGGAGCATCCGCGCAAGGCGCCGTACAACACCCTGGTG 
CTGATCGACAACAACGGCGAGATCGTCCAGAAGTACCGCAAGATCATTCCCTGGTGCCCCATCGAGGGCTGG 
TATCCCGGTGGCCAGACCTACGTCAGCGAAGGGCCGAAGGGCATGAAGATCAGCCTGATCATCTGCGACGAC 
GGCAACTACCCGGAAATCTGGCGCGACTGCGCGATGAAGGGCGCCGAGCTGATCGTGCGCTGCCAGGGCTAC 
ATGTACCCGGCCAAGGACCAGCAGGTGATGATGGCCAAGGCCATGGCCTGGGCCAACAACTGCTATGTGGCG 
GTGGCCAACGCCGCCGGCTTCGACGGCGTGTATTCCTACTTCGGCCACTCGGCGATCATCGGCTTCGACGGT 
CGTACCCTGGGTGAGTGCGGCGAGGAGGAAATGGGTATCCAGTACGCCCAGCTGTCGCTCTCGCAGATCCGC 
GATGCGCGCGCCAACGATCAGTCGCAGAACCACCTGTTCAAGATCCTCCACCGCGGCTACAGCGGCTTGCAG 
GCATCCGGCGATGGCGACCGGGGCCTGGCGGAATGTCCGTTCGAGTTCTACCGCACCTGGGTCACCGACGCC 
GAGAAGGCGCGCGAGAATGTCGAGCGTCTGACCCGCTCGACTACCGGCGTGGCGCAATGCCCGGTCGGCCGG 
CTGCCCTACGAGGGACTGGAGAAGGAGGCCTGA