ViewGene
Locus Name:
GeneID:
Location:

GeneID:1812
TrivialName: PA14_60850
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60850
PAO1 Ortholog Locus:PA4599
Sequence Length:1164
Protein Length:387
Start: 5427869
Stop: 5426706
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07987.1|AE004873_9 (AE004873) RND multidrug efflux membrane fusion protein MexC precursor [Pseudomonas aeruginosa PAO1]
Identities = 380/387 (98%), Positives = 382/387 (98%)
Sequence:
ATGGCTGATTTGCGTGCAATAGGAAGGATCGGGGCGTTGGCTATGGCCATCGCGTTGGCAGGTTGTGGGCCG
GCGGAAGAGCGACAGGAGGCCGCCGAAATGGTGTTGCCGGTGGAGGTCCTGACGGTGCAGGCCGAGTCCCTG
GCGCTGAGTTCGGAACTGCCTGGGCGGATCGAACCGGTGCGGGTCGCCGAGGTGCGCGCGCGGGTGGCCGGC
ATCGTCGTGCAGAAGCGCTTCGAGGAGGGCGCCGACGTCAAGGCTGGCGACCTGCTGTTCCAGATCGATCCG
GCACCGCTGAAGGCTGCGGTGTCGCGCGCCGAGGGTGAGCTGGCGCGGAACCGCGCGGTGCTGTTCGAGGCG
CAGGCGCGGGTGCGCCGCTACGAGCCGCTGGTGAAGATCCAGGCGGTCAGTCAGCAGGACTTCGATACCGCC
ACCGCCGACCTGCGCAGCGCCGAGGCGGCGACCCGCTCGGCCCAGGCCGACCTGGAGACCGCGCGCCTGAAC
CTCGGCTACGCCTCGGTCACTGCGCCGATCTCCGGGCGCATCGGCCGCGCGCTGGTGACCGAGGGCGCGCTG
GTCGGGCAGGGCGAGGCGACGCTGATGGCGCGCATCCAGCAGCTCGATCCGATCTATGCGGATTTCACCCAG
ACCGCGGCCGAGGCCCTGCGCCTGCGCGACGCCCTGAAGAAAGGCACCTTGGCCGCCGGCGACAGCCAGGCG
CTGACCCTGCGCGTCGAAGGGACGCCCTACGAGCGCCAGGGCGCGTTGCAGTTCGCCGACGTGGCGGTGGAT
CGCGGCACCGGCCAGATCGCCCTGCGCGGCAAGTTCGCCAACCCCGACGGGGTCCTGCTGCCGGGCATGTAC
GTGCGCGTACGTACGCCCCAGGGCATCGACAACCAGGCGATCCTGGTGCCGCAACGGGCCGTGCGCCGCTCC
AGCGACGGCAGCGCCCAGGTGATGGTGGTGGGCGCCGACGAGCGCGCCGAGGCGCGCAGCGTCGGTACCGGC
GTCATGCAGGGGTCGCGCTGGCAGATCACCGAGGGCCTGGAGCCGGGTGACCGGGTCATAGTCGGCGGCCTG
GCTGCGGTGCAGCCGGGGGTGAAGATCGTGCCGAAGCCGGATGGTGCCCAGACGCAAGCCCAGTCGTCCGTG
CCGCAACAGTAA
Translation:
MADLRAIGRIGALAMAIALAGCGPAEERQEAAEMVLPVEVLTVQAESLALSSELPGRIEPVRVAEVRARVAG
IVVQKRFEEGADVKAGDLLFQIDPAPLKAAVSRAEGELARNRAVLFEAQARVRRYEPLVKIQAVSQQDFDTA
TADLRSAEAATRSAQADLETARLNLGYASVTAPISGRIGRALVTEGALVGQGEATLMARIQQLDPIYADFTQ
TAAEALRLRDALKKGTLAAGDSQALTLRVEGTPYERQGALQFADVAVDRGTGQIALRGKFANPDGVLLPGMY
VRVRTPQGIDNQAILVPQRAVRRSSDGSAQVMVVGADERAEARSVGTGVMQGSRWQITEGLEPGDRVIVGGL
AAVQPGVKIVPKPDGAQTQAQSSVPQQ*
AnnotationID:1821GeneID:1812
AnnotatorUID: diggins
Modification Date/Time: 2005-05-06 06:06:37
Gene Name:mexC
Confidence Code:1
GeneProduct:multidrug efflux RND membrane fusion protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(3) Antibiotic resistance and susceptibility
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00529,COG0845
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN68425.1|AE016475_4 (AE016784) multidrug efflux RND membrane fusion protein MexC
           [Pseudomonas putida KT2440]
          Length = 378

 Score =  512 bits (1319), Expect = e-144
 Identities = 268/363 (73%), Positives = 309/363 (85%), Gaps = 2/363 (0%)

>gb|AAO56097.1| (AE016865) multidrug resistance protein, AcrA/AcrE family
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 386

 Score =  458 bits (1178), Expect = e-128
 Identities = 239/383 (62%), Positives = 299/383 (78%), Gaps = 2/383 (0%)

>gb|AAN79059.1|AE016756_242 (AE016756) Acriflavine resistance protein A precursor [Escherichia
           coli CFT073]
          Length = 409

 Score =  327 bits (838), Expect = 8e-89
 Identities = 172/380 (45%), Positives = 253/380 (66%), Gaps = 6/380 (1%)






 
Structural Features:
pfam00529, HlyD, HlyD family secretion protein.
CD-Length = 309 residues, 100.0% aligned
Score =  138 bits (348), Expect = 3e-33
Identities = 110/310 (35%), Positives = 156/310 (50%), Gaps = 15/310 (4%)

COG0845, AcrA, Membrane-fusion protein 
[Cell envelope biogenesis, outer membrane]
CD-Length = 372 residues,  99.2% aligned
Score =  104 bits (259), Expect = 4e-23
Identities = 144/380 (37%), Positives = 197/380 (51%), Gaps = 15/380 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3627




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20774




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31623




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1812_cdd.html


    		




 
URL:

















    ReferenceID:33271




  
Author/Investigator(s):
        Okamoto K, Gotoh N, Nishino T.





  
Title:
        Extrusion of penem antibiotics by multicomponent efflux systems MexAB-OprM, MexCD-OprJ, and MexXY-OprM of Pseudomonas aeruginosa.




  
PubMed:
        12121960


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 2002 Aug;46(8):2696-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12121960

















    ReferenceID:33272




  
Author/Investigator(s):
        De Kievit TR, Parkins MD, Gillis RJ, Srikumar R, Ceri H, Poole K, Iglewski BH, Storey DG.





  
Title:
        Multidrug efflux pumps: expression patterns and contribution to antibiotic resistance in Pseudomonas aeruginosa biofilms.




  
PubMed:
        11353623


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 2001 Jun;45(6):1761-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11353623

















    ReferenceID:33273




  
Author/Investigator(s):
        Gotoh N, Tsujimoto H, Nomura A, Okamoto K, Tsuda M, Nishino T.





  
Title:
        Functional replacement of OprJ by OprM in the MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa.




  
PubMed:
        9711836


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1998 Aug 1;165(1):21-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9711836

















    ReferenceID:33274




  
Author/Investigator(s):
        Gotoh N, Tsujimoto H, Tsuda M, Okamoto K, Nomura A, Wada T, Nakahashi M, Nishino T.





  
Title:
        Characterization of the MexC-MexD-OprJ multidrug efflux system in DeltamexA-mexB-oprM mutants of Pseudomonas aeruginosa.




  
PubMed:
        9687387


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1998 Aug;42(8):1938-43.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9687387

















    ReferenceID:33275




  
Author/Investigator(s):
        Srikumar R, Kon T, Gotoh N, Poole K.





  
Title:
        Expression of Pseudomonas aeruginosa multidrug efflux pumps MexA-MexB-OprM and MexC-MexD-OprJ in a multidrug-sensitive Escherichia coli strain.




  
PubMed:
        9449262


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1998 Jan;42(1):65-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9449262

















    ReferenceID:33276




  
Author/Investigator(s):
        Poole K, Gotoh N, Tsujimoto H, Zhao Q, Wada A, Yamasaki T, Neshat S, Yamagishi J, Li XZ, Nishino T.





  
Title:
        Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa.




  
PubMed:
        8878035


MedLine:
        




    
Source:
Mol Microbiol. 1996 Aug;21(4):713-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8878035









           
Homologs By Global Alignment 

           

       



     
Gene ID:1812
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3183
                           PA4599_tr
                           translation of PA4599
                           387
                           98.19
                           98.70
                           0
                           1859.0
     

     
     
98841
                           gb|AAG07987.1|AE004873_9
                           (AE004873) RND multidrug efflux membrane fusion protein MexC precursor [Pseudomonas aeruginosa PAO1]
                           387
                           98.19
                           98.70
                           0
                           1859.0
     

     
     
98842
                           gb|AAB41956.1|
                           (U57969) membrane fusion protein MexC [Pseudomonas aeruginosa]
                           387
                           98.19
                           98.70
                           0
                           1859.0
     

     
     
98843
                           gb|AAN68425.1|AE016475_4
                           (AE016784) multidrug efflux RND membrane fusion protein MexC [Pseudomonas putida KT2440]
                           388
                           70.36
                           81.70
                           11
                           1321.0
     

     
     
98844
                           emb|CAD24338.1|
                           (AJ431260) mexC [uncultured bacterium]
                           391
                           65.72
                           79.02
                           7
                           1228.0
     

     






      

          
GC ORFID: 44814How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4599Glimmer Score: 

          
Start: 4691549Stop: 4690386

          
Length: 1164


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4599 translation of PA4599Homolog Bit Score 720.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene220 (ORF: BLASTX 453.0), t_PA0425 (ORF: BLASTX 282.0), t_PA2019 (ORF: BLASTX 277.0), psyr_15may02_Scaffold7_revised_gene7334 (ORF: BLASTX 267.0), psyr_15may02_Scaffold5_revised_gene6177 (ORF: BLASTX 207.0), t_PA4599 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTGATTTGCGTGCAATAGGAAGGATCGGGGCGTTGGCTATGGCCATCGCGTTGGCAGGTTGTGGGCCG 
GCGGAAGAGCGACAGGAGGCCGCCGAAATGGTGTTGCCGGTGGAGGTCCTGACGGTGCAGGCCGAGTCCCTG 
GCGCTGAGTTCGGAACTGCCTGGGCGGATCGAACCGGTGCGGGTCGCCGAGGTGCGCGCGCGGGTGGCCGGC 
ATCGTCGTGCAGAAGCGCTTCGAGGAGGGCGCCGACGTCAAGGCTGGCGACCTGCTGTTCCAGATCGATCCG 
GCACCGCTGAAGGCTGCGGTGTCGCGCGCCGAGGGTGAGCTGGCGCGGAACCGCGCGGTGCTGTTCGAGGCG 
CAGGCGCGGGTGCGCCGCTACGAGCCGCTGGTGAAGATCCAGGCGGTCAGTCAGCAGGACTTCGATACCGCC 
ACCGCCGACCTGCGCAGCGCCGAGGCGGCGACCCGCTCGGCCCAGGCCGACCTGGAGACCGCGCGCCTGAAC 
CTCGGCTACGCCTCGGTCACTGCGCCGATCTCCGGGCGCATCGGCCGCGCGCTGGTGACCGAGGGCGCGCTG 
GTCGGGCAGGGCGAGGCGACGCTGATGGCGCGCATCCAGCAGCTCGATCCGATCTATGCGGATTTCACCCAG 
ACCGCGGCCGAGGCCCTGCGCCTGCGCGACGCCCTGAAGAAAGGCACCTTGGCCGCCGGCGACAGCCAGGCG 
CTGACCCTGCGCGTCGAAGGGACGCCCTACGAGCGCCAGGGCGCGTTGCAGTTCGCCGACGTGGCGGTGGAT 
CGCGGCACCGGCCAGATCGCCCTGCGCGGCAAGTTCGCCAACCCCGACGGGGTCCTGCTGCCGGGCATGTAC 
GTGCGCGTACGTACGCCCCAGGGCATCGACAACCAGGCGATCCTGGTGCCGCAACGGGCCGTGCGCCGCTCC 
AGCGACGGCAGCGCCCAGGTGATGGTGGTGGGCGCCGACGAGCGCGCCGAGGCGCGCAGCGTCGGTACCGGC 
GTCATGCAGGGGTCGCGCTGGCAGATCACCGAGGGCCTGGAGCCGGGTGACCGGGTCATAGTCGGCGGCCTG 
GCTGCGGTGCAGCCGGGGGTGAAGATCGTGCCGAAGCCGGATGGTGCCCAGACGCAAGCCCAGTCGTCCGTG 
CCGCAACAGTAA