ViewGene
Locus Name:
GeneID:
Location:

GeneID:1810
TrivialName: PA14_35520
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_35520
PAO1 Ortholog Locus:PA2248
Sequence Length:1053
Protein Length:350
Start: 3158399
Stop: 3157347
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05636.1|AE004650_7 (AE004650) 2-oxoisovalerate dehydrogenase (beta subunit) [Pseudomonas aeruginosa PAO1]
Identities = 350/350 (100%), Positives = 350/350 (100%)
Sequence:
ATGAATGCCATGAACCCGCAACACGAGAACGCCCAGACGGTCACCAGCATGACCATGATCCAGGCGCTGCGC
TCGGCGATGGACATCATGCTCGAGCGCGACGACGACGTGGTGGTATTCGGCCAGGACGTCGGCTACTTCGGC
GGCGTGTTCCGCTGCACCGAAGGCCTGCAGAAGAAATACGGCACCTCGCGGGTGTTCGATGCGCCGATCTCC
GAGAGCGGCATCATCGGCGCCGCGGTCGGCATGGGCGCCTACGGCCTGCGCCCGGTGGTGGAGATCCAGTTC
GCCGACTACGTCTACCCGGCCTCCGACCAGTTGATCTCCGAGGCGGCGCGCCTGCGCTATCGCTCGGCCGGC
GACTTCATCGTGCCGATGACCGTACGCATGCCCTGTGGCGGCGGCATCTACGGCGGGCAAACGCACAGCCAG
AGCCCGGAGGCGATGTTCACCCAGGTCTGCGGCCTGCGCACGGTGATGCCGTCCAACCCCTACGACGCCAAG
GGCCTGCTGATCGCCTGCATCGAGAACGACGACCCGGTGATCTTCCTCGAGCCCAAGCGCCTCTACAACGGC
CCGTTCGATGGCCACCACGACCGCCCGGTGACGCCCTGGTCCAAGCATCCGGCCAGCCAGGTGCCGGACGGC
TACTACAAGGTGCCGCTGGACAAGGCGGCGATCGTCCGCCCCGGCGCGGCGCTGACCGTGCTGACCTACGGC
ACCATGGTCTACGTGGCCCAGGCCGCAGCCGACGAGACCGGCCTGGACGCCGAGATCATCGACCTGCGCAGC
CTCTGGCCGCTGGACCTGGAAACCATCGTCGCCTCGGTGAAGAAGACCGGCCGCTGCGTCATCGCCCACGAG
GCGACCCGCACCTGCGGGTTCGGCGCCGAGCTGATGTCGCTGGTGCAGGAGCACTGCTTCCACCACCTGGAG
GCGCCGATCGAGCGCGTCACCGGTTGGGACACCCCCTACCCGCATGCCCAGGAGTGGGCGTATTTCCCCGGC
CCCGCGCGCGTCGGCGCGGCATTCAAGCGTGTGATGGAGGTCTGA
Translation:
MNAMNPQHENAQTVTSMTMIQALRSAMDIMLERDDDVVVFGQDVGYFGGVFRCTEGLQKKYGTSRVFDAPIS
ESGIIGAAVGMGAYGLRPVVEIQFADYVYPASDQLISEAARLRYRSAGDFIVPMTVRMPCGGGIYGGQTHSQ
SPEAMFTQVCGLRTVMPSNPYDAKGLLIACIENDDPVIFLEPKRLYNGPFDGHHDRPVTPWSKHPASQVPDG
YYKVPLDKAAIVRPGAALTVLTYGTMVYVAQAAADETGLDAEIIDLRSLWPLDLETIVASVKKTGRCVIAHE
ATRTCGFGAELMSLVQEHCFHHLEAPIERVTGWDTPYPHAQEWAYFPGPARVGAAFKRVMEV*
AnnotationID:1819GeneID:1810
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 06:06:18
Gene Name:bkdA2
Confidence Code:2
GeneProduct:2-oxoisovalerate dehydrogenase, beta subunit"
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:37 kDa keto acid dehydrogenase E1-beta subunit
Functional Category Confidence Code:5
COGs:COG0022
Secondary Functional Category(ies):energy metabolism
EC Number:1.2.4.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAA65615.1| (M57613) 39 kDa keto acid dehydrogenase E1-beta subunit
           [Pseudomonas putida]
          Length = 352

 Score =  633 bits (1633), Expect = 0.0
 Identities = 301/349 (86%), Positives = 324/349 (92%), Gaps = 2/349 (0%)

>gb|AAL44285.1| (AE009277) 2-oxoisovalerate dehydrogenase beta subunit
           [Agrobacterium tumefaciens str. C58 (U. Washington)]
          Length = 337

 Score =  536 bits (1382), Expect = e-152
 Identities = 252/333 (75%), Positives = 288/333 (86%)

>gb|AAN33717.1|AE014549_2 (AE014549) 2-oxoisovalerate dehydrogenase, E1 component, beta
           subunit [Brucella suis 1330]
          Length = 337

 Score =  528 bits (1361), Expect = e-149
 Identities = 248/336 (73%), Positives = 284/336 (84%)








 
Structural Features:
COG0022, AcoB, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dehydrogenase (E1) component, eukaryotic type, beta subunit 
[Energy production and conversion]
CD-Length = 324 residues,  99.7% aligned
Score =  489 bits (1260), Expect = 2e-139
Identities = 189/339 (55%), Positives = 236/339 (69%), Gaps = 21/339 (6%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3623




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20770




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32527




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1810_cdd.html


    		




 
URL:

















    ReferenceID:34442




  
Author/Investigator(s):
        Burns G, Brown T, Hatter K, Idriss JM, Sokatch JR.





  
Title:
        Similarity of the E1 subunits of branched-chain-oxoacid dehydrogenase from Pseudomonas putida to the corresponding subunits of mammalian branched-chain-oxoacid and pyruvate dehydrogenases.




  
PubMed:
        3416875


MedLine:
        




    
Source:
Eur J Biochem. 1988 Sep 15;176(2):311-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3416875









           
Homologs By Global Alignment 

           

       



     
Gene ID:1810
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3180
                           PA2248_tr
                           translation of PA2248
                           350
                           99.99
                           99.99
                           0
                           1858.0
     

     
     
98731
                           gb|AAG05636.1|AE004650_7
                           (AE004650) 2-oxoisovalerate dehydrogenase (beta subunit) [Pseudomonas aeruginosa PAO1]
                           350
                           99.99
                           99.99
                           0
                           1858.0
     

     
     
98732
                           gb|AAA65615.1|
                           (M57613) 39 kDa keto acid dehydrogenase E1-beta subunit [Pseudomonas putida]
                           354
                           85.02
                           91.52
                           6
                           1633.0
     

     
     
98733
                           gb|AAN69980.1|AE016636_3
                           (AE016790) 2-oxoisovalerate dehydrogenase, beta subunit [Pseudomonas putida KT2440]
                           350
                           84.85
                           90.57
                           11
                           1621.0
     

     
     
98734
                           gb|AAA65616.1|
                           (M57613) 37 kDa keto acid dehydrogenase E1-beta subunit [Pseudomonas putida]
                           350
                           84.57
                           90.57
                           11
                           1616.0
     

     






      

          
GC ORFID: 44812How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2248Glimmer Score: 

          
Start: 1795517Stop: 1796569

          
Length: 1053


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2248 translation of PA2248Homolog Bit Score 720.0

          
Other Homologs:  t_PA3416 (ORF: BLASTX 255.0), t_PA2248 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAATGCCATGAACCCGCAACACGAGAACGCCCAGACGGTCACCAGCATGACCATGATCCAGGCGCTGCGC 
TCGGCGATGGACATCATGCTCGAGCGCGACGACGACGTGGTGGTATTCGGCCAGGACGTCGGCTACTTCGGC 
GGCGTGTTCCGCTGCACCGAAGGCCTGCAGAAGAAATACGGCACCTCGCGGGTGTTCGATGCGCCGATCTCC 
GAGAGCGGCATCATCGGCGCCGCGGTCGGCATGGGCGCCTACGGCCTGCGCCCGGTGGTGGAGATCCAGTTC 
GCCGACTACGTCTACCCGGCCTCCGACCAGTTGATCTCCGAGGCGGCGCGCCTGCGCTATCGCTCGGCCGGC 
GACTTCATCGTGCCGATGACCGTACGCATGCCCTGTGGCGGCGGCATCTACGGCGGGCAAACGCACAGCCAG 
AGCCCGGAGGCGATGTTCACCCAGGTCTGCGGCCTGCGCACGGTGATGCCGTCCAACCCCTACGACGCCAAG 
GGCCTGCTGATCGCCTGCATCGAGAACGACGACCCGGTGATCTTCCTCGAGCCCAAGCGCCTCTACAACGGC 
CCGTTCGATGGCCACCACGACCGCCCGGTGACGCCCTGGTCCAAGCATCCGGCCAGCCAGGTGCCGGACGGC 
TACTACAAGGTGCCGCTGGACAAGGCGGCGATCGTCCGCCCCGGCGCGGCGCTGACCGTGCTGACCTACGGC 
ACCATGGTCTACGTGGCCCAGGCCGCAGCCGACGAGACCGGCCTGGACGCCGAGATCATCGACCTGCGCAGC 
CTCTGGCCGCTGGACCTGGAAACCATCGTCGCCTCGGTGAAGAAGACCGGCCGCTGCGTCATCGCCCACGAG 
GCGACCCGCACCTGCGGGTTCGGCGCCGAGCTGATGTCGCTGGTGCAGGAGCACTGCTTCCACCACCTGGAG 
GCGCCGATCGAGCGCGTCACCGGTTGGGACACCCCCTACCCGCATGCCCAGGAGTGGGCGTATTTCCCCGGC 
CCCGCGCGCGTCGGCGCGGCATTCAAGCGTGTGATGGAGGTCTGA