ViewGene
Locus Name:
GeneID:
Location:

GeneID:1801
TrivialName: PA14_60420
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60420
PAO1 Ortholog Locus:PA4565
Sequence Length:1119
Protein Length:372
Start: 5387063
Stop: 5385945
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07953.1|AE004870_4 (AE004870) glutamate 5-kinase [Pseudomonas aeruginosa PAO1]
Identities = 372/372 (100%), Positives = 372/372 (100%)
Sequence:
ATGCGGGACAAGGTGACTGGCGCGCGGCGCTGGGTGGTGAAGATCGGTAGCGCCCTGCTGACGGCCGATGGC
CGTGGGCTGGATCGCAATGCCATGGCCGTTTGGGTCGAGCAGATGGTGGCGCTGCATTGCGCCGGCATCGAG
CTGGTGCTGGTGTCGTCCGGCGCCGTCGCCGCCGGTATGAGCCGCCTGGGCTGGGTGTCCCGACCTAGCGCG
ATGCACGAACTCCAGGCCGCCGCCTCGGTGGGACAGATGGGGTTGGTGCAGGCCTGGGAGTCCAGCTTCGCC
CTGCATGGCCTGCAGACCGCCCAGGTGTTGCTGACCCATGACGACCTCTCCGACCGCAAGCGCTACCTGAAC
GCCCGCAGCACCCTGCGGACCCTGGTCGAGCTGGGCGTGGTCCCGGTGATCAACGAGAACGACACGGTGGTC
ACCGACGAGATCCGCTTCGGCGACAACGACACCCTGGCGGCACTGGTGGCCAACCTGGTCGAGGCCGACCTG
CTGGTGATCCTTACCGATCGCGACGGTATGTTCGACGCCGATCCGCGCAACAATCCCGATGCCCAACTGATC
TACGAGGCGCGTGCCGATGATCCGCAGCTCGATGCGGTGGCCGGCGGCAGTGCCGGCGCCCTGGGGCGCGGC
GGAATGCAGACCAAGCTGCGGGCGGCGCGCCTGGCGGCGCGTTCCGGCGGGCATACGGTGATCGTCGGCGGG
CGTATCGAGCGTGTCCTCGACCGCCTGCGCGCCGGCGAACGCCTCGGCACCCTGTTGACCCCTGATCGCAGT
CGCAAGGCGGCGCGCAAGCAGTGGCTGGCCGGCCACTTGCAGATGCGCGGCACCCTGGTGCTGGACGATGGG
GCGGTGAAGGCGGTGAGCCAGGATCACAAGAGCCTGTTGCCGGTTGGGGTCAAGGCGGTCCAGGGCAGCTTC
CGTCGCGGCGAGATGGTGGTCTGTGTGGACCAGGGCGGGCGCGAAGTGGCGCGCGGCCTGGTCAACTACAGT
GCGCTGGAGGCGCAGAAGATCCTCGGTCAGCCGACGGATGCCATCGAGGCCTTGCTCGGTTACGTGGATGGG
CCGGAGCTGGTGCATCGCGACAATCTGGTTCTGGTCTGA
Translation:
MRDKVTGARRWVVKIGSALLTADGRGLDRNAMAVWVEQMVALHCAGIELVLVSSGAVAAGMSRLGWVSRPSA
MHELQAAASVGQMGLVQAWESSFALHGLQTAQVLLTHDDLSDRKRYLNARSTLRTLVELGVVPVINENDTVV
TDEIRFGDNDTLAALVANLVEADLLVILTDRDGMFDADPRNNPDAQLIYEARADDPQLDAVAGGSAGALGRG
GMQTKLRAARLAARSGGHTVIVGGRIERVLDRLRAGERLGTLLTPDRSRKAARKQWLAGHLQMRGTLVLDDG
AVKAVSQDHKSLLPVGVKAVQGSFRRGEMVVCVDQGGREVARGLVNYSALEAQKILGQPTDAIEALLGYVDG
PELVHRDNLVLV*
AnnotationID:1810GeneID:1801
AnnotatorUID: diggins
Modification Date/Time: 2006-02-06 01:01:17
Gene Name:proB
Confidence Code:2
GeneProduct:glutamate 5-kinase
Cell Localization:(1) Cytoplasmic
Synonyms:
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0263,KOG1154,pfam00696
Secondary Functional Category(ies):
EC Number:2.7.2.11
Status:ACTIVE
Pathway:
Proline biosynthesis; first step.
Homology:
>gb|AAO54342.1| (AE016858) glutamate 5-kinase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 372

 Score =  630 bits (1625), Expect = e-180
 Identities = 321/372 (86%), Positives = 342/372 (91%)

>gb|AAN78870.1|AE016756_53 (AE016756) Glutamate 5-kinase [Escherichia coli CFT073]
          Length = 380

 Score =  296 bits (758), Expect = 1e-79
 Identities = 156/359 (43%), Positives = 227/359 (63%), Gaps = 1/359 (0%)
Structural Features:
COG0263, ProB, Glutamate 5-kinase [Amino acid transport and metabolism]
CD-Length = 369 residues, 100.0% aligned
Score =  469 bits (1209), Expect = 5e-133
Identities = 207/371 (55%), Positives = 268/371 (72%), Gaps = 3/371 (0%)

KOG1154, Gamma-glutamyl kinase [Amino acid transport and metabolism]
CD-Length = 285 residues,  96.8% aligned
Score =  202 bits (515), Expect = 1e-52
Identities = 103/278 (37%), Positives = 159/278 (57%), Gaps = 17/278 (6%)

pfam00696, AA_kinase, Amino acid kinase family.
CD-Length = 236 residues,  99.6% aligned
Score =  141 bits (356), Expect = 3e-34
Identities = 73/243 (30%), Positives = 112/243 (46%), Gaps = 21/243 (8%)
Genomic Context:






   
Comment:


















    ReferenceID:3605




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20752




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31648




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1801_cdd.html


    		




 
URL:

















    ReferenceID:33049




  
Author/Investigator(s):
        Jeenes DJ, Soldati L, Baur H, Watson JM, Mercenier A, Reimmann C, Leisinger T, Haas D.





  
Title:
        Expression of biosynthetic genes from Pseudomonas aeruginosa and Escherichia coli in the heterologous host.




  
PubMed:
        3018429


MedLine:
        




    
Source:
Mol Gen Genet. 1986 Jun;203(3):421-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3018429

















    ReferenceID:33050




  
Author/Investigator(s):
        Wretlind B, Pavlovskis OR.





  
Title:
        Genetic mapping and characterization of Pseudomonas aeruginosa mutants defective in the formation of extracellular proteins.




  
PubMed:
        6427194


MedLine:
        




    
Source:
J Bacteriol. 1984 Jun;158(3):801-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6427194

















    ReferenceID:33064




  
Author/Investigator(s):
        Jochimsen B, Nygaard P, Vestergaard T.





  
Title:
        Location on the chromosome of Escherichia coli of genes governing purine metabolism. Adenosine deaminase (add), guanosine kinase (gsk) and hypoxanthine phosphoribosyltransferase (hpt).




  
PubMed:
        765747


MedLine:
        




    
Source:
Mol Gen Genet. 1975 Dec 30;143(1):85-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=765747

















    ReferenceID:33065




  
Author/Investigator(s):
        Hayzer DJ.





  
Title:
        Sub-cloning of the wild-type proAB region of the Escherichia coli genome.




  
PubMed:
        6317797


MedLine:
        




    
Source:
J Gen Microbiol. 1983 Oct;129 (Pt 10):3215-25.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6317797

















    ReferenceID:33066




  
Author/Investigator(s):
        Deutch AH, Rushlow KE, Smith CJ.





  
Title:
        Analysis of the Escherichia coli proBA locus by DNA and protein sequencing.




  
PubMed:
        6089111


MedLine:
        




    
Source:
Nucleic Acids Res. 1984 Aug 10;12(15):6337-55.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6089111

















    ReferenceID:33067




  
Author/Investigator(s):
        Csonka LN, Gelvin SB, Goodner BW, Orser CS, Siemieniak D, Slightom JL.





  
Title:
        Nucleotide sequence of a mutation in the proB gene of Escherichia coli that confers proline overproduction and enhanced tolerance to osmotic stress.




  
PubMed:
        2841193


MedLine:
        




    
Source:
Gene. 1988 Apr 29;64(2):199-205.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2841193

















    ReferenceID:33068




  
Author/Investigator(s):
        Campanile C, Forlani G, Basso AL, Marasco R, Ricca E, Sacco M, Ferrara L, De Felice M.





  
Title:
        Identification and characterization of the proBA operon of Streptococcus bovis.




  
PubMed:
        8382022


MedLine:
        




    
Source:
Appl Environ Microbiol. 1993 Feb;59(2):519-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8382022

















    ReferenceID:33069




  
Author/Investigator(s):
        Kosuge T, Tabata K, Hoshino T.





  
Title:
        Molecular cloning and sequence analysis of the proBA operon from an extremely thermophilic eubacterium Thermus thermophilus.




  
PubMed:
        7988899


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 Oct 15;123(1-2):55-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7988899

















    ReferenceID:33070




  
Author/Investigator(s):
        Stamm LV, Barnes NY.





  
Title:
        Nucleotide sequences of the proA and proB genes of Treponema pallidum, the syphilis agent.




  
PubMed:
        9522123


MedLine:
        




    
Source:
DNA Seq. 1997;8(1-2):63-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9522123

















    ReferenceID:33071




  
Author/Investigator(s):
        Limauro D, Falciatore A, Basso AL, Forlani G, De Felice M.





  
Title:
        Proline biosynthesis in Streptococcus thermophilus: characterization of the proBA operon and its products.




  
PubMed:
        8969524


MedLine:
        




    
Source:
Microbiology. 1996 Nov;142 ( Pt 11):3275-82.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8969524









           
Homologs By Global Alignment 

           

       



     
Gene ID:1801
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3170
                           PA4565_tr
                           translation of PA4565
                           372
                           99.99
                           99.99
                           0
                           1865.0
     

     
     
98505
                           gb|AAG07953.1|AE004870_4
                           (AE004870) glutamate 5-kinase [Pseudomonas aeruginosa PAO1]
                           372
                           99.99
                           99.99
                           0
                           1865.0
     

     
     
98506
                           gb|AAO54342.1|
                           (AE016858) glutamate 5-kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           372
                           86.29
                           91.93
                           0
                           1625.0
     

     
     
98507
                           gb|AAN66316.1|AE016260_2
                           (AE016776) glutamate 5-kinase [Pseudomonas putida KT2440]
                           372
                           85.48
                           91.66
                           0
                           1595.0
     

     
     
98508
                           emb|CAD16526.1|
                           (AL646072) PROBABLE GLUTAMATE 5-KINASE PROTEIN [Ralstonia solanacearum]
                           374
                           62.03
                           76.20
                           2
                           1176.0
     

     






      

          
GC ORFID: 44803How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4565Glimmer Score: 

          
Start: 4650742Stop: 4649624

          
Length: 1119


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4565 translation of PA4565Homolog Bit Score 723.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3529 (ORF: BLASTX 627.0), t_PA4565 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGGGACAAGGTGACTGGCGCGCGGCGCTGGGTGGTGAAGATCGGTAGCGCCCTGCTGACGGCCGATGGC 
CGTGGGCTGGATCGCAATGCCATGGCCGTTTGGGTCGAGCAGATGGTGGCGCTGCATTGCGCCGGCATCGAG 
CTGGTGCTGGTGTCGTCCGGCGCCGTCGCCGCCGGTATGAGCCGCCTGGGCTGGGTGTCCCGACCTAGCGCG 
ATGCACGAACTCCAGGCCGCCGCCTCGGTGGGACAGATGGGGTTGGTGCAGGCCTGGGAGTCCAGCTTCGCC 
CTGCATGGCCTGCAGACCGCCCAGGTGTTGCTGACCCATGACGACCTCTCCGACCGCAAGCGCTACCTGAAC 
GCCCGCAGCACCCTGCGGACCCTGGTCGAGCTGGGCGTGGTCCCGGTGATCAACGAGAACGACACGGTGGTC 
ACCGACGAGATCCGCTTCGGCGACAACGACACCCTGGCGGCACTGGTGGCCAACCTGGTCGAGGCCGACCTG 
CTGGTGATCCTTACCGATCGCGACGGTATGTTCGACGCCGATCCGCGCAACAATCCCGATGCCCAACTGATC 
TACGAGGCGCGTGCCGATGATCCGCAGCTCGATGCGGTGGCCGGCGGCAGTGCCGGCGCCCTGGGGCGCGGC 
GGAATGCAGACCAAGCTGCGGGCGGCGCGCCTGGCGGCGCGTTCCGGCGGGCATACGGTGATCGTCGGCGGG 
CGTATCGAGCGTGTCCTCGACCGCCTGCGCGCCGGCGAACGCCTCGGCACCCTGTTGACCCCTGATCGCAGT 
CGCAAGGCGGCGCGCAAGCAGTGGCTGGCCGGCCACTTGCAGATGCGCGGCACCCTGGTGCTGGACGATGGG 
GCGGTGAAGGCGGTGAGCCAGGATCACAAGAGCCTGTTGCCGGTTGGGGTCAAGGCGGTCCAGGGCAGCTTC 
CGTCGCGGCGAGATGGTGGTCTGTGTGGACCAGGGCGGGCGCGAAGTGGCGCGCGGCCTGGTCAACTACAGT 
GCGCTGGAGGCGCAGAAGATCCTCGGTCAGCCGACGGATGCCATCGAGGCCTTGCTCGGTTACGTGGATGGG 
CCGGAGCTGGTGCATCGCGACAATCTGGTTCTGGTCTGA