ViewGene
Locus Name:
GeneID:
Location:

GeneID:1800
TrivialName: PA14_10240
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10240
PAO1 Ortholog Locus:PA4152
Sequence Length:1113
Protein Length:370
Start: 882262
Stop: 881150
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07539.1|AE004831_11 (AE004831) probable hydrolase [Pseudomonas aeruginosa PAO1]
Identities = 368/370 (99%), Positives = 369/370 (99%)
Sequence:
ATGAGCCGGATCCATACACTGACCATGCCCAAGTGGGGCCTGTCGATGACCGAGGGTCGGGTGGACGCCTGG
CTCAAGGAAGAGGGCGCGCATATCGAGAAAGGCGACGAGGTGCTCGACGTGGAGACCGACAAGATCTCCAGC
AGCGTCGAGGCGCCCTTCTCGGGCGTGCTGCGGCGCAAGGTGGCGCGCGAGGACGAGACCCTGGCGGTCGGC
GCGCTGCTCGGCGTGGTGGTCGAGGGCGAGGCGAGCGACGAGGAGATCGACGCACTGGTGCAACGCTTCCAG
GCCGAATTCGTACCCGCCGACGAGGACGCTGGGGATAGCGGACCGGCACCGCGGAAAGTCGAGCTGGACGGG
CGCCTCCTGCGCTATTTCGACCGCGGCGAGGGCGATCCGGCGCTACTGCTGATCCACGGTTTCGGTGGCGAC
CTGAACAACTGGTTGTTCAACCACGAGGCGCTGGCCGCCGAGCGGCGGGTGATCGCTCTAGACCTGCCGGGC
CACGGCGAGTCCGGCAAGGCCCTGGTGCGCGGCGACCTCGATGAACTCAGCGGCAGCGTGCTGGCCCTGCTC
GACCACCTCGACCTGGAACAGGTACACCTGGCCGGTCACTCCATGGGGGGAGCGGTGGCGCTGAACTGCGCG
CGCCTGGCGCCGCAGCGGGTGCTGTCGCTGAGCCTGATCGGCAGCGCCGGCCTCGGCGAGGAGATCAACGGC
GACTACCTGCGCGGCTTCGTCGAGGCGGCCAACCGCAACGCGTTGAAGCCGCAACTGGTGCAGTTGTTCTCC
GACCCCGAGCTGGTCACCCGGCAGATGCTCGAGGACATGCTCCGCTACAAGCGCCTGGAAGGCGTCGATGCG
GCCCTGCGCCAGTTGCTGGACAACCTGTTCGCCGACGGTCGCCAGCGCAACGACCTGCGTGCCGTGGCGAGC
GAGGGCCGCCAGCCGGTCCTGGCGATCTGGGGCAGCGATGACGCGATCATCCCGGCGCGGCATGCCGAGGGC
CTGCCAGCCCAGGTGGAGATCATTCCCGGACAGGCGCACATGGTGCAGATGGAGGCCGCCGAGCAGGTCAAC
CGCCTGCTGCTCGACTTCCTCCGCCAGCATTGA
Translation:
MSRIHTLTMPKWGLSMTEGRVDAWLKEEGAHIEKGDEVLDVETDKISSSVEAPFSGVLRRKVAREDETLAVG
ALLGVVVEGEASDEEIDALVQRFQAEFVPADEDAGDSGPAPRKVELDGRLLRYFDRGEGDPALLLIHGFGGD
LNNWLFNHEALAAERRVIALDLPGHGESGKALVRGDLDELSGSVLALLDHLDLEQVHLAGHSMGGAVALNCA
RLAPQRVLSLSLIGSAGLGEEINGDYLRGFVEAANRNALKPQLVQLFSDPELVTRQMLEDMLRYKRLEGVDA
ALRQLLDNLFADGRQRNDLRAVASEGRQPVLAIWGSDDAIIPARHAEGLPAQVEIIPGQAHMVQMEAAEQVN
RLLLDFLRQH*
AnnotationID:1809GeneID:1800
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 16:04:14
Gene Name:
Confidence Code:3
GeneProduct:putative hydrolase
Cell Localization:()
Synonyms:acoC
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:putative dihydrolipoamide acetyltransferase
Functional Category Confidence Code:5
COGs:COG0596,pfam00561
Secondary Functional Category(ies):energy production and conversion
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66180.1|AE016245_9 (AE016776) acetoin dehydrogenase, dihydrolipoamide
           acetyltransferase component [Pseudomonas putida KT2440]
          Length = 368

 Score =  540 bits (1391), Expect = e-153
 Identities = 277/370 (74%), Positives = 308/370 (83%), Gaps = 2/370 (0%)








 
Structural Features:
COG0596, MhpC, Predicted hydrolases or acyltransferases 
(alpha/beta hydrolase superfamily)
CD-Length = 282 residues,  98.6% aligned
Score =  111 bits (276), Expect = 1e-25
Identities = 89/284 (31%), Positives = 112/284 (39%), Gaps = 27/284 (9%)

pfam00561, Abhydrolase_1, alpha/beta hydrolase fold.
CD-Length = 224 residues, 100.0% aligned
Score = 99.5 bits (247), Expect = 6e-22
Identities = 71/224 (31%), Positives = 100/224 (44%), Gaps = 17/224 (7%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3603




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20750




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36889




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1800_cdd.html


    		




 
URL:

















    ReferenceID:36983




  
Author/Investigator(s):
        Priefert H, Hein S, Kruger N, Zeh K, Schmidt B, Steinbuchel A.





  
Title:
        Identification and molecular characterization of the Alcaligenes eutrophus H16 aco operon genes involved in acetoin catabolism.




  
PubMed:
        2061286


MedLine:
        




    
Source:
J Bacteriol. 1991 Jul;173(13):4056-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2061286

















    ReferenceID:36984




  
Author/Investigator(s):
        Kruger N, Oppermann FB, Lorenzl H, Steinbuchel A.





  
Title:
        Biochemical and molecular characterization of the Clostridium magnum acetoin dehydrogenase enzyme system.




  
PubMed:
        8206840


MedLine:
        




    
Source:
J Bacteriol. 1994 Jun;176(12):3614-30.

Erratum in:
    * J Bacteriol 1994 Aug;176(16):5193.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8206840

















    ReferenceID:36991




  
Author/Investigator(s):
        Oppermann FB, Steinbuchel A.





  
Title:
        Identification and molecular characterization of the aco genes encoding the Pelobacter carbinolicus acetoin dehydrogenase enzyme system.




  
PubMed:
        8110297


MedLine:
        




    
Source:
J Bacteriol. 1994 Jan;176(2):469-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8110297

















    ReferenceID:36993




  
Author/Investigator(s):
        Huang M, Oppermann FB, Steinbuchel A.





  
Title:
        Molecular characterization of the Pseudomonas putida 2,3-butanediol catabolic pathway.




  
PubMed:
        7813883


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 Dec 1;124(2):141-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95113288&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:1800
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3169
                           PA4152_tr
                           translation of PA4152
                           370
                           99.45
                           99.72
                           0
                           1866.0
     

     
     
98499
                           gb|AAG07539.1|AE004831_11
                           (AE004831) probable hydrolase [Pseudomonas aeruginosa PAO1]
                           370
                           99.45
                           99.72
                           0
                           1866.0
     

     
     
98500
                           gb|AAN66180.1|AE016245_9
                           (AE016776) acetoin dehydrogenase, dihydrolipoamide acetyltransferase component [Pseudomonas putida KT2440]
                           372
                           76.07
                           84.67
                           6
                           1396.0
     

     
     
98501
                           gb|AAB58981.1|
                           (L35343) dihydrolipoamide acetyltransferase [Pseudomonas putida]
                           375
                           74.66
                           83.20
                           10
                           1356.0
     

     
     
98502
                           gb|AAA21950.1|
                           (M66060) FMP [Ralstonia eutropha]
                           383
                           46.99
                           60.57
                           22
                           818.0
     

     






      

          
GC ORFID: 44802How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4152Glimmer Score: 

          
Start: 4071675Stop: 4072787

          
Length: 1113


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4152 translation of PA4152Homolog Bit Score 723.0

          
Other Homologs:  t_PA4152 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCGGATCCATACACTGACCATGCCCAAGTGGGGCCTGTCGATGACCGAGGGTCGGGTGGACGCCTGG 
CTCAAGGAAGAGGGCGCGCATATCGAGAAAGGCGACGAGGTGCTCGACGTGGAGACCGACAAGATCTCCAGC 
AGCGTCGAGGCGCCCTTCTCGGGCGTGCTGCGGCGCAAGGTGGCGCGCGAGGACGAGACCCTGGCGGTCGGC 
GCGCTGCTCGGCGTGGTGGTCGAGGGCGAGGCGAGCGACGAGGAGATCGACGCACTGGTGCAACGCTTCCAG 
GCCGAATTCGTACCCGCCGACGAGGACGCTGGGGATAGCGGACCGGCACCGCGGAAAGTCGAGCTGGACGGG 
CGCCTCCTGCGCTATTTCGACCGCGGCGAGGGCGATCCGGCGCTACTGCTGATCCACGGTTTCGGTGGCGAC 
CTGAACAACTGGTTGTTCAACCACGAGGCGCTGGCCGCCGAGCGGCGGGTGATCGCTCTAGACCTGCCGGGC 
CACGGCGAGTCCGGCAAGGCCCTGGTGCGCGGCGACCTCGATGAACTCAGCGGCAGCGTGCTGGCCCTGCTC 
GACCACCTCGACCTGGAACAGGTACACCTGGCCGGTCACTCCATGGGGGGAGCGGTGGCGCTGAACTGCGCG 
CGCCTGGCGCCGCAGCGGGTGCTGTCGCTGAGCCTGATCGGCAGCGCCGGCCTCGGCGAGGAGATCAACGGC 
GACTACCTGCGCGGCTTCGTCGAGGCGGCCAACCGCAACGCGTTGAAGCCGCAACTGGTGCAGTTGTTCTCC 
GACCCCGAGCTGGTCACCCGGCAGATGCTCGAGGACATGCTCCGCTACAAGCGCCTGGAAGGCGTCGATGCG 
GCCCTGCGCCAGTTGCTGGACAACCTGTTCGCCGACGGTCGCCAGCGCAACGACCTGCGTGCCGTGGCGAGC 
GAGGGCCGCCAGCCGGTCCTGGCGATCTGGGGCAGCGATGACGCGATCATCCCGGCGCGGCATGCCGAGGGC 
CTGCCAGCCCAGGTGGAGATCATTCCCGGACAGGCGCACATGGTGCAGATGGAGGCCGCCGAGCAGGTCAAC 
CGCCTGCTGCTCGACTTCCTCCGCCAGCATTGA