ViewGene
Locus Name:
GeneID:
Location:

GeneID:1791
TrivialName: PA14_26010
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_26010
PAO1 Ortholog Locus:PA2940
Sequence Length:1140
Protein Length:379
Start: 2272242
Stop: 2273381
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06328.1|AE004720_4 (AE004720) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
Identities = 375/379 (98%), Positives = 376/379 (99%)
Sequence:
ATGTCGCCGATTTCAGTCGTTATCGCCGGTTACGCCCGTTCGCCGTTCCACTTCGCCAGGAAAGGCGCGCTG
GTCGATATCCGCCCGGATGACCTGGCCGCCGCGGTGCTCAAGGGCCTGGTGGAGAAACTCGATCTCGACCCT
GTTCAGCTGGAGGACGTGGTCATGGGCTGCGCCTATCCGGAGGCGGAACAGGGCATGAACATCGCGCGCATC
GCCAGTTTCCGCGCCGGCTTCCCGCAGAGCCTCGGCGGCGCGACCCTCAACCGTTTCTGCGGTTCGTCCATG
AGCGCTGTCCACTACGCCGCCGGGCAAGTCCTGCTGGGTGCCGGAGAAGCCTTCATCGCCGCCGGGGTGGAG
TCGATGACCCGGGTGCCGATGGGCGGCTTCAACCTGTCGCCCAATCCCGCGTTGCTGCAGGACTATCCGGCG
GTATACATGAGCATGGGGCAGACCGCCGAGAATGTCGCCGAGCGCTACGCCGTCAGCCGCGTCGAGCAGGAA
GAGATGGCCGTACGCTCCCACGCCAAGGCAGTCGCCGCGCGCGAGGCCGGCTTGCTGCGCGAGGAGATCGTC
GCCATCGACACCCCTGCCGGCCGGGTCGCCGAGGATGGCTGCATCCGCCCGGGTACCAACCTGGAGAGCCTA
GCCCAACTGAAGCCGGCGTTCGGCGGCAGCGTCACCGCCGCCACCTCGTCGCCGCTCACCGATGGCAGCGCC
GCGCTGCTGGTCTGTAGCGAGGAATTCGCGCGCCGCCACGGGCTGGCGATCCTGGCGCGGATCAAGGCGGTG
GCGGTGGCCGGTTGTGCGCCGGAGATCATGGGCATGGGTCCGGTGCAGGCGACGCGCAAGGTTCTGCAACGG
GCCGGGCTGGGCATCGCCGACATCGACCTGGTGGAGATCAACGAGGCCTTCGCCAGCCAATCCATCGCCTGC
ATCCGTGAACTGGGGCTGGACATGGACAGGATCAACCTGGATGGCGGCGCCCTGGCCATCGGCCATCCGCTG
GGCGCCACCGGCGCGCGGATCACCGGCAAGGCCGCCGCGTTGCTGCGGCGCACCGGCGGGCGCTATGCCATC
GCCACCCAGTGCATCGCCGGCGGCCAGGGCGTGGCGACCCTGCTGGAAGCGGTGGAGTGA
Translation:
MSPISVVIAGYARSPFHFARKGALVDIRPDDLAAAVLKGLVEKLDLDPVQLEDVVMGCAYPEAEQGMNIARI
ASFRAGFPQSLGGATLNRFCGSSMSAVHYAAGQVLLGAGEAFIAAGVESMTRVPMGGFNLSPNPALLQDYPA
VYMSMGQTAENVAERYAVSRVEQEEMAVRSHAKAVAAREAGLLREEIVAIDTPAGRVAEDGCIRPGTNLESL
AQLKPAFGGSVTAATSSPLTDGSAALLVCSEEFARRHGLAILARIKAVAVAGCAPEIMGMGPVQATRKVLQR
AGLGIADIDLVEINEAFASQSIACIRELGLDMDRINLDGGALAIGHPLGATGARITGKAAALLRRTGGRYAI
ATQCIAGGQGVATLLEAVE*
AnnotationID:1800GeneID:1791
AnnotatorUID: diggins
Modification Date/Time: 2005-04-08 13:01:42
Gene Name:
Confidence Code:3
GeneProduct:acyl-CoA thiolase
Cell Localization:()
Synonyms:phaA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:beta-ketothiolase
Functional Category Confidence Code:5
COGs:cd00751,COG0183,pfam00108
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM48101.1|AF353206_1 (AF353206) beta-ketothiolase [Azospirillum brasilense]
          Length = 387

 Score =  486 bits (1251), Expect = e-137
 Identities = 242/375 (64%), Positives = 298/375 (79%), Gaps = 2/375 (0%)

>gb|AAF12018.1|AE002077_1 (AE002077) acetyl-CoA acetyltransferase [Deinococcus radiodurans]
          Length = 399

 Score =  353 bits (906), Expect = 1e-96
 Identities = 185/384 (48%), Positives = 252/384 (65%), Gaps = 14/384 (3%)








 
Structural Features:
cd00751, thiolase
CD-Length = 386 residues, 100.0% aligned
Score =  421 bits (1085), Expect = 5e-119

COG0183, PaaJ, Acetyl-CoA acetyltransferase [Lipid metabolism]
CD-Length = 392 residues,  99.2% aligned
Score =  282 bits (722), Expect = 6e-77
Identities = 157/393 (39%), Positives = 213/393 (54%), Gaps = 24/393 (6%)

pfam00108, Thiolase, N-terminal domain.
CD-Length = 264 residues,  98.9% aligned
Score =  187 bits (476), Expect = 2e-48
Identities = 105/264 (39%), Positives = 146/264 (55%), Gaps = 24/264 (9%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3585




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20732




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36112




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1791_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1791
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3155
                           PA2940_tr
                           translation of PA2940
                           379
                           98.94
                           99.20
                           0
                           1869.0
     

     
     
97995
                           gb|AAG06328.1|AE004720_4
                           (AE004720) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
                           379
                           98.94
                           99.20
                           0
                           1869.0
     

     
     
97996
                           gb|AAM48101.1|AF353206_1
                           (AF353206) beta-ketothiolase [Azospirillum brasilense]
                           388
                           62.62
                           77.83
                           10
                           1251.0
     

     
     
97997
                           gb|AAF12018.1|AE002077_1
                           (AE002077) acetyl-CoA acetyltransferase [Deinococcus radiodurans] [Deinococcus radiodurans R1]
                           401
                           46.13
                           62.84
                           24
                           909.0
     

     
     
97998
                           dbj|BAB07206.1|
                           (AP001519) acetyl-CoA C-acyltransferase [Bacillus halodurans]
                           395
                           44.30
                           65.06
                           18
                           890.0
     

     






      

          
GC ORFID: 44793How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2940Glimmer Score: 

          
Start: 2681683Stop: 2680544

          
Length: 1140


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2940 translation of PA2940Homolog Bit Score 724.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8045 (ORF: BLASTX 281.0), t_PA3013 (ORF: BLASTX 276.0), t_PA0228 (ORF: BLASTX 276.0), t_PA3454 (ORF: BLASTX 268.0), psyr_15may02_Scaffold5_revised_gene6193 (ORF: BLASTX 263.0), t_PA2001 (ORF: BLASTX 256.0), t_PA3589 (ORF: BLASTX 253.0), psyr_15may02_Scaffold15_revised_gene2524 (ORF: BLASTX 249.0), t_PA2940 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGCCGATTTCAGTCGTTATCGCCGGTTACGCCCGTTCGCCGTTCCACTTCGCCAGGAAAGGCGCGCTG 
GTCGATATCCGCCCGGATGACCTGGCCGCCGCGGTGCTCAAGGGCCTGGTGGAGAAACTCGATCTCGACCCT 
GTTCAGCTGGAGGACGTGGTCATGGGCTGCGCCTATCCGGAGGCGGAACAGGGCATGAACATCGCGCGCATC 
GCCAGTTTCCGCGCCGGCTTCCCGCAGAGCCTCGGCGGCGCGACCCTCAACCGTTTCTGCGGTTCGTCCATG 
AGCGCTGTCCACTACGCCGCCGGGCAAGTCCTGCTGGGTGCCGGAGAAGCCTTCATCGCCGCCGGGGTGGAG 
TCGATGACCCGGGTGCCGATGGGCGGCTTCAACCTGTCGCCCAATCCCGCGTTGCTGCAGGACTATCCGGCG 
GTATACATGAGCATGGGGCAGACCGCCGAGAATGTCGCCGAGCGCTACGCCGTCAGCCGCGTCGAGCAGGAA 
GAGATGGCCGTACGCTCCCACGCCAAGGCAGTCGCCGCGCGCGAGGCCGGCTTGCTGCGCGAGGAGATCGTC 
GCCATCGACACCCCTGCCGGCCGGGTCGCCGAGGATGGCTGCATCCGCCCGGGTACCAACCTGGAGAGCCTA 
GCCCAACTGAAGCCGGCGTTCGGCGGCAGCGTCACCGCCGCCACCTCGTCGCCGCTCACCGATGGCAGCGCC 
GCGCTGCTGGTCTGTAGCGAGGAATTCGCGCGCCGCCACGGGCTGGCGATCCTGGCGCGGATCAAGGCGGTG 
GCGGTGGCCGGTTGTGCGCCGGAGATCATGGGCATGGGTCCGGTGCAGGCGACGCGCAAGGTTCTGCAACGG 
GCCGGGCTGGGCATCGCCGACATCGACCTGGTGGAGATCAACGAGGCCTTCGCCAGCCAATCCATCGCCTGC 
ATCCGTGAACTGGGGCTGGACATGGACAGGATCAACCTGGATGGCGGCGCCCTGGCCATCGGCCATCCGCTG 
GGCGCCACCGGCGCGCGGATCACCGGCAAGGCCGCCGCGTTGCTGCGGCGCACCGGCGGGCGCTATGCCATC 
GCCACCCAGTGCATCGCCGGCGGCCAGGGCGTGGCGACCCTGCTGGAAGCGGTGGAGTGA