ViewGene
Locus Name:
GeneID:
Location:

GeneID:1787
TrivialName: PA14_70680
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70680
PAO1 Ortholog Locus:PA5354
Sequence Length:1080
Protein Length:359
Start: 6295122
Stop: 6294043
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08739.1|AE004947_9 (AE004947) glycolate oxidase subunit GlcE [Pseudomonas aeruginosa PAO1]
Identities = 355/359 (98%), Positives = 356/359 (99%)
Sequence:
ATGAGCCAGCCGTATTTCGATCCGGACGCTGGCGCCGACCATGCCGGCGCGCTGCTCGAGCGGGTCCGCGAC
GCCCTGGCGCGGCGCGCGCCGCTGCGCATCGTCGGCGGCGATAGCAAGGCGTTCCTCGGCAGGCCGGTCGCG
GGCGAACCGCTGGCGCTCGCCGAACACCGCGGGATCGTCGCCTACGATCCCTGCGAGCTGGTGATCACCGCC
CGCGCCGGTACGCCGCTGGACGAGCTACAGGCCGTGCTGGCGCGGGGCGGGCAGATGCTTGCCTGCGAGGCG
CCGGGTTTCGGCGGCCGGGCGACCGTCGGCGGGGTGCTCGCCAGCGGCCTGTCCGGACCGCGCCGGCCCTGG
GCGGGAGCGGTACGCGATTTCGTCCTCGGCACGCGGCTGATCAGTGGCCTGGGCACCCACCTGCGCTTCGGC
GGCGAGGTGATGAAGAACGTCGCCGGCTACGACCTGTCGCGGTTGCTGGCGGGCAGCTTCGGTTGCCTCGGG
GTGGTCACCGAAGTGTCCTTCAAGGTGCTGCCCATGCCGCGCTGCCAGGCCGGCCTGAGCCTGGACCTGCCG
GTGGCGACGGCGTTGCGCAAGCTGGCCGAGTGGGGAGGCCAGCCATTGCCGATCAGTGCGGCCTGCCACGAC
GGCGAAGCCCTGCGCCTGCGCCTGGAGGGCGGCGAGGGCTCGGTACGCTCGGCGCGCGACCGCCTCGGTGGC
GAAGCGCTCGAGGACGGCTTCTGGGCCGACCTGCGCGAGCAGCGCCTGCCGTTCTTCGACGACCCGCGACCG
CTCTGGCGCCTGTCGCTGCCGGCGCTGGCGCCGGAGACGCCGTTGCCCGGTCGTCAGTTGCTGGACTGGGGC
GGGGCGCTGCGCTGGCTGAAGAGCGACGCGCCGGCCGCGGACATCCGCGCGTGGACCGCCGCCCATGGCGGC
CACGCCACCTGCTACCGCGCCGGCCATCTCGATGCGCCGTTCCAGCCGCTACCGCCTGGACTGCTCGACCTG
CACCGGGCACTCAAACGCCAGCTCGACCCGCAGGGCATCTTCAATCCCGGGCGGATGTACGCCGAACTCTGA
Translation:
MSQPYFDPDAGADHAGALLERVRDALARRAPLRIVGGDSKAFLGRPVAGEPLALAEHRGIVAYDPCELVITA
RAGTPLDELQAVLARGGQMLACEAPGFGGRATVGGVLASGLSGPRRPWAGAVRDFVLGTRLISGLGTHLRFG
GEVMKNVAGYDLSRLLAGSFGCLGVVTEVSFKVLPMPRCQAGLSLDLPVATALRKLAEWGGQPLPISAACHD
GEALRLRLEGGEGSVRSARDRLGGEALEDGFWADLREQRLPFFDDPRPLWRLSLPALAPETPLPGRQLLDWG
GALRWLKSDAPAADIRAWTAAHGGHATCYRAGHLDAPFQPLPPGLLDLHRALKRQLDPQGIFNPGRMYAEL*
AnnotationID:1796GeneID:1787
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 07:07:17
Gene Name:glcE
Confidence Code:2
GeneProduct:glycolate oxidase subunit GlcE
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0277,pfam01565
Secondary Functional Category(ies):energy metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57031.1| (AE016868) glycolate oxidase, subunit GlcE [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 352

 Score =  457 bits (1175), Expect = e-128
 Identities = 231/347 (66%), Positives = 263/347 (75%), Gaps = 1/347 (0%)

>gb|AAN69341.1|AE016571_7 (AE016788) glycolate oxidase, subunit GlcE [Pseudomonas putida
           KT2440]
          Length = 350

 Score =  447 bits (1151), Expect = e-125
 Identities = 227/347 (65%), Positives = 264/347 (76%), Gaps = 2/347 (0%)

>gb|AAB02531.1| (L43490) glycolate oxidase subunit [Escherichia coli]
          Length = 350

 Score =  414 bits (1064), Expect = e-115
 Identities = 209/347 (60%), Positives = 252/347 (72%), Gaps = 2/347 (0%)








 
Structural Features:
COG0277, GlcD, FAD/FMN-containing dehydrogenases [Energy production and conversion]
CD-Length = 459 residues,  90.6% aligned
Score =  143 bits (360), Expect = 3e-35
Identities = 103/417 (24%), Positives = 144/417 (34%), Gaps = 76/417 (18%)

pfam01565, FAD_binding_4, FAD binding domain. 
CD-Length = 158 residues,  94.9% aligned
Score = 81.5 bits (201), Expect = 1e-16
Identities = 52/154 (33%), Positives = 65/154 (42%), Gaps = 5/154 (3%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3577




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20724




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34751




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1787_cdd.html


    		




 
URL:

















    ReferenceID:35683




  
Author/Investigator(s):
        Pellicer MT, Badia J, Aguilar J, Baldoma L.





  
Title:
        glc locus of Escherichia coli: characterization of genes encoding the subunits of glycolate oxidase and the glc regulator protein.




  
PubMed:
        8606183


MedLine:
        




    
Source:
J Bacteriol. 1996 Apr;178(7):2051-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8606183









           
Homologs By Global Alignment 

           

       



     
Gene ID:1787
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3151
                           PA5354_tr
                           translation of PA5354
                           359
                           98.88
                           99.16
                           0
                           1871.0
     

     
     
97961
                           gb|AAG08739.1|AE004947_9
                           (AE004947) glycolate oxidase subunit GlcE [Pseudomonas aeruginosa PAO1]
                           359
                           98.88
                           99.16
                           0
                           1871.0
     

     
     
97962
                           gb|AAO57031.1|
                           (AE016868) glycolate oxidase, subunit GlcE [Pseudomonas syringae pv. tomato str. DC3000]
                           359
                           64.90
                           73.53
                           7
                           1173.0
     

     
     
97963
                           gb|AAN69341.1|AE016571_7
                           (AE016788) glycolate oxidase, subunit GlcE [Pseudomonas putida KT2440]
                           359
                           63.23
                           73.53
                           9
                           1148.0
     

     
     
97964
                           gb|AAB02531.1|
                           (L43490) glycolate oxidase subunit [Escherichia coli]
                           359
                           58.21
                           70.19
                           9
                           1057.0
     

     






      

          
GC ORFID: 44789How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5354Glimmer Score: 

          
Start: 5558811Stop: 5557732

          
Length: 1080


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5354 translation of PA5354Homolog Bit Score 725.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8166 (ORF: BLASTX 467.0), t_PA5354 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAGCCGTATTTCGATCCGGACGCTGGCGCCGACCATGCCGGCGCGCTGCTCGAGCGGGTCCGCGAC 
GCCCTGGCGCGGCGCGCGCCGCTGCGCATCGTCGGCGGCGATAGCAAGGCGTTCCTCGGCAGGCCGGTCGCG 
GGCGAACCGCTGGCGCTCGCCGAACACCGCGGGATCGTCGCCTACGATCCCTGCGAGCTGGTGATCACCGCC 
CGCGCCGGTACGCCGCTGGACGAGCTACAGGCCGTGCTGGCGCGGGGCGGGCAGATGCTTGCCTGCGAGGCG 
CCGGGTTTCGGCGGCCGGGCGACCGTCGGCGGGGTGCTCGCCAGCGGCCTGTCCGGACCGCGCCGGCCCTGG 
GCGGGAGCGGTACGCGATTTCGTCCTCGGCACGCGGCTGATCAGTGGCCTGGGCACCCACCTGCGCTTCGGC 
GGCGAGGTGATGAAGAACGTCGCCGGCTACGACCTGTCGCGGTTGCTGGCGGGCAGCTTCGGTTGCCTCGGG 
GTGGTCACCGAAGTGTCCTTCAAGGTGCTGCCCATGCCGCGCTGCCAGGCCGGCCTGAGCCTGGACCTGCCG 
GTGGCGACGGCGTTGCGCAAGCTGGCCGAGTGGGGAGGCCAGCCATTGCCGATCAGTGCGGCCTGCCACGAC 
GGCGAAGCCCTGCGCCTGCGCCTGGAGGGCGGCGAGGGCTCGGTACGCTCGGCGCGCGACCGCCTCGGTGGC 
GAAGCGCTCGAGGACGGCTTCTGGGCCGACCTGCGCGAGCAGCGCCTGCCGTTCTTCGACGACCCGCGACCG 
CTCTGGCGCCTGTCGCTGCCGGCGCTGGCGCCGGAGACGCCGTTGCCCGGTCGTCAGTTGCTGGACTGGGGC 
GGGGCGCTGCGCTGGCTGAAGAGCGACGCGCCGGCCGCGGACATCCGCGCGTGGACCGCCGCCCATGGCGGC 
CACGCCACCTGCTACCGCGCCGGCCATCTCGATGCGCCGTTCCAGCCGCTACCGCCTGGACTGCTCGACCTG 
CACCGGGCACTCAAACGCCAGCTCGACCCGCAGGGCATCTTCAATCCCGGGCGGATGTACGCCGAACTCTGA