ViewGene
Locus Name:
GeneID:
Location:

GeneID:1786
TrivialName: PA14_67990
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67990
PAO1 Ortholog Locus:PA5147
Sequence Length:1068
Protein Length:355
Start: 6068121
Stop: 6069188
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08532.1|AE004927_10 (AE004927) A / G specific adenine glycosylase [Pseudomonas aeruginosa PAO1]
Identities = 354/355 (99%), Positives = 354/355 (99%)
Sequence:
ATGACACCTGAAGGCTTCAACGGCGCGGTCCTCGACTGGTACGACCGTCACGGTCGCAAGGACCTGCCCTGG
CAGCAGGGCATCACCCCCTACCGGGTGTGGGTCTCGGAAATCATGCTGCAACAGACCCAGGTCAGCACCGTG
CTCGGTTACTTCGACCGTTTCATGGCAGCGTTGCCCGACGTCGAGGCACTGGCCGCGGCGGCCGAGGACGAA
GTCCTGCACCTGTGGACCGGGCTCGGCTACTACAGCCGTGCGCGCAACCTGCACAAGACCGCGCAGATCGTG
GTCGAGCGGCATGCCGGGGAGTTCCCCCGCGACGTCGAGCAACTCGCCGAACTGCCCGGCATCGGCCGCTCC
ACCGCTGGAGCCATCGCCAGCCTGTCGATGGGCCTGCGCGCACCGATCCTCGACGGCAACGTCAAGCGCGTA
CTGGCGCGCTACCTGGCGCAGGACGGCTATCCCGGCGAACCGAAGGTGGCCAGGGCGCTGTGGGAAGCCGCC
GAGCGCTTCACCCCGCACGCACGGGTCAACCACTACACCCAGGCGATGATGGACCTCGGCGCCACCCTCTGC
ACGCGCAGCAAGCCCAGTTGCCTGCTTTGCCCGCTGGTCTCCGGCTGCCGCGCGCACCTGCTCGGCCGCGAA
GCCGATTATCCGCAGCCCAAGCCGCGCAAGGCGTTGCCGCAGAAGCGCACGCTGATGCCGATCCTGGCCAAC
CGCGACGGCGCCATACTGCTCTACCGGCGGCCTTCCAGCGGACTCTGGGGCGGGCTCTGGAGCCTCCCCGAA
CTGGACGACCTCGACGGCCTCGAACCGCTCGCCGCGCGCCATTCCCTGGCCCTCGGCGAGCGCCGCGAACTG
AGCGGCCTGACCCATACCTTCAGTCATTTCCAGCTCGCCATCGAGCCCTGGCTGGTGGCGGTGGAAGGCGCT
CCGCGCGCCGTGGCCGAGGGCGACTGGCTCTGGTATAACCTCGCCACCCCGCCGCGCCTGGGCCTCGCCGCT
CCGGTGAAGAAGCTGCTCAAGCGCGCAGAACAGGAACTCGGACGCGGCACGACCGCGTGA
Translation:
MTPEGFNGAVLDWYDRHGRKDLPWQQGITPYRVWVSEIMLQQTQVSTVLGYFDRFMAALPDVEALAAAAEDE
VLHLWTGLGYYSRARNLHKTAQIVVERHAGEFPRDVEQLAELPGIGRSTAGAIASLSMGLRAPILDGNVKRV
LARYLAQDGYPGEPKVARALWEAAERFTPHARVNHYTQAMMDLGATLCTRSKPSCLLCPLVSGCRAHLLGRE
ADYPQPKPRKALPQKRTLMPILANRDGAILLYRRPSSGLWGGLWSLPELDDLDGLEPLAARHSLALGERREL
SGLTHTFSHFQLAIEPWLVAVEGAPRAVAEGDWLWYNLATPPRLGLAAPVKKLLKRAEQELGRGTTA*
AnnotationID:1795GeneID:1786
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 13:01:26
Gene Name:mutY
Confidence Code:1
GeneProduct:A/G-specific adenine glycosylase
Cell Localization:()
Synonyms:micA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1194,cd00056,smart00478,pfam00730
Secondary Functional Category(ies):
EC Number:3.2.2.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58768.1| (AE016875) A/G-specific adenine glycosylase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 355

 Score =  581 bits (1498), Expect = e-165
 Identities = 283/355 (79%), Positives = 304/355 (85%)

>gb|AAN65917.1|AE016219_10 (AE016775) A/G specific adenine glycosylase [Pseudomonas putida
           KT2440]
          Length = 355

 Score =  577 bits (1487), Expect = e-164
 Identities = 278/352 (78%), Positives = 299/352 (84%)

>gb|AAC75998.1| (AE000378) adenine glycosylase; G.C --> T.A transversions
           [Escherichia coli K12]
          Length = 350

 Score =  370 bits (951), Expect = e-102
 Identities = 187/344 (54%), Positives = 232/344 (67%), Gaps = 1/344 (0%)






 
Structural Features:
COG1194, MutY, A/G-specific DNA glycosylase 
[DNA replication, recombination, and repair]
CD-Length = 342 residues,  98.2% aligned
Score =  395 bits (1016), Expect = 4e-111
Identities = 182/347 (52%), Positives = 223/347 (64%), Gaps = 12/347 (3%)

cd00056, ENDO3c, endonuclease III
CD-Length = 158 residues, 100.0% aligned
Score =  145 bits (367), Expect = 7e-36
Identities = 59/162 (36%), Positives = 80/162 (49%), Gaps = 8/162 (4%)

smart00478, ENDO3c, endonuclease III
CD-Length = 149 residues, 100.0% aligned
Score =  133 bits (335), Expect = 4e-32
Identities = 46/153 (30%), Positives = 71/153 (46%), Gaps = 5/153 (3%)

pfam00730, HhH-GPD, HhH-GPD superfamily base excision DNA repair protein.
CD-Length = 145 residues, 100.0% aligned
Score =  103 bits (258), Expect = 3e-23
Identities = 50/145 (34%), Positives = 74/145 (51%), Gaps = 9/145 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3575




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20722




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34915




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1786_cdd.html


    		




 
URL:

















    ReferenceID:35779




  
Author/Investigator(s):
        Oliver A, Sanchez JM, Blazquez J.





  
Title:
        Characterization of the GO system of Pseudomonas aeruginosa.




  
PubMed:
        12445642


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2002 Nov 19;217(1):31-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12445642

















    ReferenceID:35780




  
Author/Investigator(s):
        Michaels ML, Pham L, Nghiem Y, Cruz C, Miller JH.





  
Title:
        MutY, an adenine glycosylase active on G-A mispairs, has homology to endonuclease III.




  
PubMed:
        2197596


MedLine:
        




    
Source:
Nucleic Acids Res. 1990 Jul 11;18(13):3841-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2197596

















    ReferenceID:35781




  
Author/Investigator(s):
        Tsai-Wu JJ, Radicella JP, Lu AL.





  
Title:
        Nucleotide sequence of the Escherichia coli micA gene required for A/G-specific mismatch repair: identity of micA and mutY.




  
PubMed:
        2001994


MedLine:
        




    
Source:
J Bacteriol. 1991 Mar;173(6):1902-10.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2001994









           
Homologs By Global Alignment 

           

       



     
Gene ID:1786
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3150
                           PA5147_tr
                           translation of PA5147
                           355
                           99.71
                           99.71
                           0
                           1871.0
     

     
     
97868
                           gb|AAG08532.1|AE004927_10
                           (AE004927) A / G specific adenine glycosylase [Pseudomonas aeruginosa PAO1]
                           355
                           99.71
                           99.71
                           0
                           1871.0
     

     
     
97869
                           gb|AAO58768.1|
                           (AE016875) A/G-specific adenine glycosylase [Pseudomonas syringae pv. tomato str. DC3000]
                           355
                           79.71
                           85.63
                           0
                           1498.0
     

     
     
97870
                           gb|AAN65917.1|AE016219_10
                           (AE016775) A/G specific adenine glycosylase [Pseudomonas putida KT2440]
                           355
                           78.30
                           84.22
                           0
                           1485.0
     

     
     
97881
                           gb|AAO70575.1|
                           (AE016844) A/G-specific adenine glycosylase [Salmonella enterica subsp. enterica serovar Typhi Ty2]
                           356
                           53.93
                           65.44
                           7
                           931.0
     

     






      

          
GC ORFID: 44788How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5147Glimmer Score: 

          
Start: 5331807Stop: 5332874

          
Length: 1068


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5147 translation of PA5147Homolog Bit Score 725.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6943 (ORF: BLASTX 578.0), t_PA5146 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACACCTGAAGGCTTCAACGGCGCGGTCCTCGACTGGTACGACCGTCACGGTCGCAAGGACCTGCCCTGG 
CAGCAGGGCATCACCCCCTACCGGGTGTGGGTCTCGGAAATCATGCTGCAACAGACCCAGGTCAGCACCGTG 
CTCGGTTACTTCGACCGTTTCATGGCAGCGTTGCCCGACGTCGAGGCACTGGCCGCGGCGGCCGAGGACGAA 
GTCCTGCACCTGTGGACCGGGCTCGGCTACTACAGCCGTGCGCGCAACCTGCACAAGACCGCGCAGATCGTG 
GTCGAGCGGCATGCCGGGGAGTTCCCCCGCGACGTCGAGCAACTCGCCGAACTGCCCGGCATCGGCCGCTCC 
ACCGCTGGAGCCATCGCCAGCCTGTCGATGGGCCTGCGCGCACCGATCCTCGACGGCAACGTCAAGCGCGTA 
CTGGCGCGCTACCTGGCGCAGGACGGCTATCCCGGCGAACCGAAGGTGGCCAGGGCGCTGTGGGAAGCCGCC 
GAGCGCTTCACCCCGCACGCACGGGTCAACCACTACACCCAGGCGATGATGGACCTCGGCGCCACCCTCTGC 
ACGCGCAGCAAGCCCAGTTGCCTGCTTTGCCCGCTGGTCTCCGGCTGCCGCGCGCACCTGCTCGGCCGCGAA 
GCCGATTATCCGCAGCCCAAGCCGCGCAAGGCGTTGCCGCAGAAGCGCACGCTGATGCCGATCCTGGCCAAC 
CGCGACGGCGCCATACTGCTCTACCGGCGGCCTTCCAGCGGACTCTGGGGCGGGCTCTGGAGCCTCCCCGAA 
CTGGACGACCTCGACGGCCTCGAACCGCTCGCCGCGCGCCATTCCCTGGCCCTCGGCGAGCGCCGCGAACTG 
AGCGGCCTGACCCATACCTTCAGTCATTTCCAGCTCGCCATCGAGCCCTGGCTGGTGGCGGTGGAAGGCGCT 
CCGCGCGCCGTGGCCGAGGGCGACTGGCTCTGGTATAACCTCGCCACCCCGCCGCGCCTGGGCCTCGCCGCT 
CCGGTGAAGAAGCTGCTCAAGCGCGCAGAACAGGAACTCGGACGCGGCACGACCGCGTGA