ViewGene
Locus Name:
GeneID:
Location:

GeneID:1784
TrivialName: PA14_32220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_32220
PAO1 Ortholog Locus:PA2509
Sequence Length:1122
Protein Length:373
Start: 2800268
Stop: 2801389
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAK52296.1| (AY026914) putative muconate cycloisomerase I CatB [Pseudomonas putida]
Identities = 373/373 (100%), Positives = 373/373 (100%)
Sequence:
ATGAGCCAAGCCGTGATCCAAAGCCTCGAGTCGATCATCGTCGACCTGCCGACCATCCGCCCGCACAAGCTG
GCCATGCACACCATGAACCGGCAGACCCTGGTGATCCTCCGCCTGCGTTGCAGCGACGGCATCGAGGGCCTC
GGCGAAGCCACCACCATCGGTGGCCTGGCCTACGGCAGCGAGAGCCCGGAGAGCATCAAGAGCAATCTCGAC
GCGCATTTCGCCCCGTTGCTGCTGGGCCAGCCGGCGGACAACGTGAATGCCGCGATGCAGCGCCTGGACCGG
CACATCAGGGGCAACACCTTCGCCCGCTCGGCGGTCGAGACCGCCTTGCTCGACGCCCATGGCAAGCGCCTC
GGCCTGGCCGTCAGCGAACTGCTCGGCGGCCGCCTGCACGACAGCCTGGAGGTGGCCTGGACCCTGGCCAGC
GGCGACACCGGCCGCGACATCGAGGAAGCCGAGCGGATGCTCGACCTGCGCCGCCACCGGCACTTCAAGCTG
AAGATCGGCGCCGGTGAGGTGGATGCCGACGTGGCCCACGCGATCGCCATCAAGCGCGCCCTTGGCGAGCGC
GCCAGCGTGCGGGTGGACGTCAACCAGGCCTGGGACGAAGGCGTTGCCCAGCGTGCCTGCGCGACGCTCGGC
GACAACGGCATCGCCCTGATCGAACAGCCCATCGCCCGCCACAACCGCATCGGCCTGGCGCGCCTGAGCAGC
CGCGGCGGCGCGCCGATCATGGCCGACGAGGCCATCGAGAGCGTCGAGGATGCCTTCCACCTGGCTCGCGAG
GGCGCCGCCCCGGTGTTCGCCCTGAAGATCGCCAAGAACGGCGGACCGCGCGCGGTGCTGCGTAGCGCGGCG
ATCGCCGAGGCGGCCGGGATCGGCCTGTACGGCGGGACCATGCTCGAGGGCGGCATCGGCACCCTGGCCGCC
GCCCACGCCTTCGTCACCCTCGACCGCCTGGCCTGGCACAGCGAACTGTTCGGCCCGTTGTTGCTGACCGAG
GACATCCTCCTCGAGTCGCCGCGCTACCAGGATTTCCACTTGCACGTGCCGCGTGCGCCGGGTCTCGGCCTG
GCGCTCGACGAGGAGCGCCTGGCGCGCTTCCGCCGGCGCTGA
Translation:
MSQAVIQSLESIIVDLPTIRPHKLAMHTMNRQTLVILRLRCSDGIEGLGEATTIGGLAYGSESPESIKSNLD
AHFAPLLLGQPADNVNAAMQRLDRHIRGNTFARSAVETALLDAHGKRLGLAVSELLGGRLHDSLEVAWTLAS
GDTGRDIEEAERMLDLRRHRHFKLKIGAGEVDADVAHAIAIKRALGERASVRVDVNQAWDEGVAQRACATLG
DNGIALIEQPIARHNRIGLARLSSRGGAPIMADEAIESVEDAFHLAREGAAPVFALKIAKNGGPRAVLRSAA
IAEAAGIGLYGGTMLEGGIGTLAAAHAFVTLDRLAWHSELFGPLLLTEDILLESPRYQDFHLHVPRAPGLGL
ALDEERLARFRRR*
AnnotationID:1793GeneID:1784
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 08:08:50
Gene Name:catB
Confidence Code:1
GeneProduct:muconate cycloisomerase I
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:muconate lactonizing enzyme
Functional Category Confidence Code:5
COGs:pfam01188
Secondary Functional Category(ies):putative enzyme
EC Number:5.5.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69312.1|AE016568_7 (AE016788) muconate cycloisomerase [Pseudomonas putida KT2440]
          Length = 373

 Score =  545 bits (1404), Expect = e-154
 Identities = 272/373 (72%), Positives = 315/373 (84%)

>gb|AAG42035.1|AF042281_4 (AF042281) putative muconate cycloisomerase [Ralstonia eutropha]
          Length = 381

 Score =  464 bits (1194), Expect = e-130
 Identities = 243/371 (65%), Positives = 286/371 (77%)

>dbj|BAB21460.1| (AB024746) muconate cycloisomerase [Burkholderia sp. NK8]
          Length = 375

 Score =  432 bits (1111), Expect = e-120
 Identities = 227/369 (61%), Positives = 273/369 (73%)







 
Structural Features:
pfam01188, MR_MLE, Mandelate racemase / muconate lactonizing enzyme
CD-Length = 239 residues,  99.6% aligned
Score =  236 bits (603), Expect = 3e-63
Identities = 114/239 (47%), Positives = 151/239 (63%), Gaps = 1/239 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3571




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20718




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33058




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1784_cdd.html


    		




 
URL:

















    ReferenceID:34505




  
Author/Investigator(s):
        Kukor JJ, Olsen RH, Ballou DP.





  
Title:
        Cloning and expression of the catA and catBC gene clusters from Pseudomonas aeruginosa PAO.




  
PubMed:
        3139626


MedLine:
        




    
Source:
J Bacteriol. 1988 Oct;170(10):4458-65.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3139626









           
Homologs By Global Alignment 

           

       



     
Gene ID:1784
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
97813
                           gb|AAK52296.1|
                           (AY026914) putative muconate cycloisomerase I CatB [Pseudomonas putida]
                           373
                           99.99
                           99.99
                           0
                           1879.0
     

     
     
3148
                           PA2509_tr
                           translation of PA2509
                           373
                           99.46
                           99.73
                           0
                           1871.0
     

     
     
97814
                           gb|AAG05897.1|AE004678_3
                           (AE004678) muconate cycloisomerase I [Pseudomonas aeruginosa PAO1]
                           373
                           99.46
                           99.73
                           0
                           1871.0
     

     
     
97815
                           dbj|BAB32456.1|
                           (AB047272) cis,cis-muconate cycloisomerase CatB [Pseudomonas sp. CA10]
                           373
                           76.67
                           87.13
                           0
                           1467.0
     

     
     
97816
                           gb|AAN69312.1|AE016568_7
                           (AE016788) muconate cycloisomerase [Pseudomonas putida KT2440]
                           373
                           72.92
                           84.45
                           0
                           1404.0
     

     






      

          
GC ORFID: 44786How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2509Glimmer Score: 

          
Start: 2153656Stop: 2152535

          
Length: 1122


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2509 translation of PA2509Homolog Bit Score 725.0

          
Other Homologs:  t_PA2509 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAAGCCGTGATCCAAAGCCTCGAGTCGATCATCGTCGACCTGCCGACCATCCGCCCGCACAAGCTG 
GCCATGCACACCATGAACCGGCAGACCCTGGTGATCCTCCGCCTGCGTTGCAGCGACGGCATCGAGGGCCTC 
GGCGAAGCCACCACCATCGGTGGCCTGGCCTACGGCAGCGAGAGCCCGGAGAGCATCAAGAGCAATCTCGAC 
GCGCATTTCGCCCCGTTGCTGCTGGGCCAGCCGGCGGACAACGTGAATGCCGCGATGCAGCGCCTGGACCGG 
CACATCAGGGGCAACACCTTCGCCCGCTCGGCGGTCGAGACCGCCTTGCTCGACGCCCATGGCAAGCGCCTC 
GGCCTGGCCGTCAGCGAACTGCTCGGCGGCCGCCTGCACGACAGCCTGGAGGTGGCCTGGACCCTGGCCAGC 
GGCGACACCGGCCGCGACATCGAGGAAGCCGAGCGGATGCTCGACCTGCGCCGCCACCGGCACTTCAAGCTG 
AAGATCGGCGCCGGTGAGGTGGATGCCGACGTGGCCCACGCGATCGCCATCAAGCGCGCCCTTGGCGAGCGC 
GCCAGCGTGCGGGTGGACGTCAACCAGGCCTGGGACGAAGGCGTTGCCCAGCGTGCCTGCGCGACGCTCGGC 
GACAACGGCATCGCCCTGATCGAACAGCCCATCGCCCGCCACAACCGCATCGGCCTGGCGCGCCTGAGCAGC 
CGCGGCGGCGCGCCGATCATGGCCGACGAGGCCATCGAGAGCGTCGAGGATGCCTTCCACCTGGCTCGCGAG 
GGCGCCGCCCCGGTGTTCGCCCTGAAGATCGCCAAGAACGGCGGACCGCGCGCGGTGCTGCGTAGCGCGGCG 
ATCGCCGAGGCGGCCGGGATCGGCCTGTACGGCGGGACCATGCTCGAGGGCGGCATCGGCACCCTGGCCGCC 
GCCCACGCCTTCGTCACCCTCGACCGCCTGGCCTGGCACAGCGAACTGTTCGGCCCGTTGTTGCTGACCGAG 
GACATCCTCCTCGAGTCGCCGCGCTACCAGGATTTCCACTTGCACGTGCCGCGTGCGCCGGGTCTCGGCCTG 
GCGCTCGACGAGGAGCGCCTGGCGCGCTTCCGCCGGCGCTGA