ViewGene
Locus Name:
GeneID:
Location:

GeneID:1777
TrivialName: PA14_23280
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_23280
PAO1 Ortholog Locus:PA3166
Sequence Length:1098
Protein Length:365
Start: 2018692
Stop: 2019789
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06554.1|AE004740_7 (AE004740) chorismate mutase [Pseudomonas aeruginosa PAO1]
Identities = 365/365 (100%), Positives = 365/365 (100%)
Sequence:
ATGGCGGACCAGGACCAGCTCAAGGCCTTGCGCTTGCGCATCGACAGCCTCGACGAGAAGTTGCTCGAGCTG
ATCAGCGAGCGTGCCCGTTGCGCCCAGGACGTGGCGCGAGTGAAGACCCAGACCCTGGGCGAGGGCGAGGCG
CCGGTGTTCTACCGTCCCGAGCGCGAAGCCTGGGTGCTCAAGCACATCATGCAATTGAACAAGGGGCCGCTG
GACAACGAAGAGGTCGCGCGGCTGTTCCGCGAGATCATGTCGTCCTGCCTGGCGCTCGAGCAGCCGCTGAAA
GTGGCCTATCTCGGACCCGAGGGCACCTTTACCCAGGCTGCGGCACTCAAGCATTTCGGCAACGCGGTGATC
AGCACGCCGATGGCGGCGATCGACGAGGTGTTCCGCGAGGTCGCCGCCGGCGCGGTGAACTTCGGCGTGGTG
CCTGTGGAAAACTCCACCGAGGGGGCGGTCAACCATACCCTCGACAGCTTCCTCGAGCACGACATGGTGATC
TGCGGCGAAGTCGAGCTGCGTATCCACCATCACCTGCTGGTCGGCGAAACCACCAAGACCGACAACATCACC
CGGATCTATTCCCATGCCCAATCCCTCGCGCAATGTCGCAAGTGGCTGGACTCGCACTATCCGAGCGTCGAG
CGGGTGGCGGTGTCGAGCAACGCCGACGCGGCCAAGCGGGTGAAGAGCGAGTGGAATTCGGCAGCGATCGCC
GGTGACATGGCGGCCAGCCTGTACGACCTGAGCAAGCTGCACGAGAAGATCGAGGACCGCCCGGACAACTCC
ACGCGCTTCCTGATCATCGGCAACCAGGAGGTTCCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCC
ATGCGCAACAAGCCGGGAGCCCTGCACGAGCTGCTGGTACCGTTCCACAACAACGGTATCGACCTGACCCGC
ATCGAAACCCGTCCGTCGCGCAGCGGCAAATGGACCTACGTGTTCTTCATCGATTTCGTCGGCCACCATAAA
GAACCGCTGATCAAGGACGTCCTGGAGAAGATCGGCCAGGAAGCCGTCGCCCTGAAGGTCCTGGGCTCCTAT
CCGAAAGCGGTGCTCTGA
Translation:
MADQDQLKALRLRIDSLDEKLLELISERARCAQDVARVKTQTLGEGEAPVFYRPEREAWVLKHIMQLNKGPL
DNEEVARLFREIMSSCLALEQPLKVAYLGPEGTFTQAAALKHFGNAVISTPMAAIDEVFREVAAGAVNFGVV
PVENSTEGAVNHTLDSFLEHDMVICGEVELRIHHHLLVGETTKTDNITRIYSHAQSLAQCRKWLDSHYPSVE
RVAVSSNADAAKRVKSEWNSAAIAGDMAASLYDLSKLHEKIEDRPDNSTRFLIIGNQEVPPTGDDKTSIIVS
MRNKPGALHELLVPFHNNGIDLTRIETRPSRSGKWTYVFFIDFVGHHKEPLIKDVLEKIGQEAVALKVLGSY
PKAVL*
AnnotationID:1786GeneID:1777
AnnotatorUID: diggins
Modification Date/Time: 2005-04-13 12:12:54
Gene Name:pheA
Confidence Code:2
GeneProduct: prephenate dehydratase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name: chorismate mutase
Functional Category Confidence Code:5
COGs:pfam00800,COG0077
Secondary Functional Category(ies):
EC Number:5.4.99.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAD47360.1|AF038578_3 (AF038578) chorismate mutase/prephenate dehydratase [Pseudomonas
           stutzeri]
          Length = 365

 Score =  652 bits (1681), Expect = 0.0
 Identities = 324/365 (88%), Positives = 349/365 (95%)

>gb|AAN67389.1|AE016365_4 (AE016780) chorismate mutase/prephenate dehydratase [Pseudomonas
           putida KT2440]
          Length = 367

 Score =  640 bits (1652), Expect = 0.0
 Identities = 320/367 (87%), Positives = 348/367 (94%), Gaps = 2/367 (0%)

>gb|AAO29204.1| (AE012558) P-protein; chorismate mutase; prephenate dehydratase
           [Xylella fastidiosa Temecula1]
          Length = 374

 Score =  346 bits (888), Expect = 1e-94
 Identities = 178/360 (49%), Positives = 253/360 (70%), Gaps = 5/360 (1%)






 
Structural Features:
pfam00800, PDT, Prephenate dehydratase. 
CD-Length = 182 residues, 100.0% aligned
Score =  234 bits (599), Expect = 1e-62
Identities = 93/182 (51%), Positives = 117/182 (64%), Gaps = 3/182 (1%)

COG0077, PheA, Prephenate dehydratase [Amino acid transport and metabolism]
CD-Length = 279 residues,  99.3% aligned
Score =  357 bits (917), Expect = 2e-99







   
Genomic Context:






   
Comment:


















    ReferenceID:3557




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20704




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32376




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1777_cdd.html


    		




 
URL:

















    ReferenceID:33930




  
Author/Investigator(s):
        Fischer RS, Zhao G, Jensen RA.





  
Title:
        Cloning, sequencing, and expression of the P-protein gene (pheA) of Pseudomonas stutzeri in Escherichia coli: implications for evolutionary relationships in phenylalanine biosynthesis.




  
PubMed:
        1919506


MedLine:
        




    
Source:
J Gen Microbiol. 1991 Jun;137 ( Pt 6):1293-301.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1919506

















    ReferenceID:33931




  
Author/Investigator(s):
        Nurk A, Kasak L, Kivisaar M.





  
Title:
        Sequence of the gene (pheA) encoding phenol monooxygenase from Pseudomonas sp. EST1001: expression in Escherichia coli and Pseudomonas putida.




  
PubMed:
        1650730


MedLine:
        




    
Source:
Gene. 1991 Jun 15;102(1):13-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1650730

















    ReferenceID:33932




  
Author/Investigator(s):
        Gavini N, Davidson BE.





  
Title:
        Regulation of pheA expression by the pheR product in Escherichia coli is mediated through attenuation of transcription.




  
PubMed:
        2019599


MedLine:
        




    
Source:
J Biol Chem. 1991 Apr 25;266(12):7750-3.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2019599

















    ReferenceID:33933




  
Author/Investigator(s):
        Follettie MT, Sinskey AJ.





  
Title:
        Molecular cloning and nucleotide sequence of the Corynebacterium glutamicum pheA gene.




  
PubMed:
        3525519


MedLine:
        




    
Source:
J Bacteriol. 1986 Aug;167(2):695-702.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3525519









           
Homologs By Global Alignment 

           

       



     
Gene ID:1777
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3141
                           PA3166_tr
                           translation of PA3166
                           365
                           99.99
                           99.99
                           0
                           1874.0
     

     
     
97691
                           gb|AAG06554.1|AE004740_7
                           (AE004740) chorismate mutase [Pseudomonas aeruginosa PAO1]
                           365
                           99.99
                           99.99
                           0
                           1874.0
     

     
     
97692
                           gb|AAD47360.1|AF038578_3
                           (AF038578) chorismate mutase/prephenate dehydratase [Pseudomonas stutzeri]
                           365
                           88.76
                           95.61
                           0
                           1681.0
     

     
     
97693
                           gb|AAN67389.1|AE016365_4
                           (AE016780) chorismate mutase/prephenate dehydratase [Pseudomonas putida KT2440]
                           367
                           87.19
                           94.82
                           2
                           1654.0
     

     
     
97694
                           gb|AAO55267.1|
                           (AE016862) chorismate mutase/prephenate dehydratase [Pseudomonas syringae pv. tomato str. DC3000]
                           365
                           86.84
                           93.97
                           1
                           1643.0
     

     






      

          
GC ORFID: 44779How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3166Glimmer Score: 

          
Start: 2935239Stop: 2934142

          
Length: 1098


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3166 translation of PA3166Homolog Bit Score 726.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7731 (ORF: BLASTX 640.0), t_PA3166 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGGACCAGGACCAGCTCAAGGCCTTGCGCTTGCGCATCGACAGCCTCGACGAGAAGTTGCTCGAGCTG 
ATCAGCGAGCGTGCCCGTTGCGCCCAGGACGTGGCGCGAGTGAAGACCCAGACCCTGGGCGAGGGCGAGGCG 
CCGGTGTTCTACCGTCCCGAGCGCGAAGCCTGGGTGCTCAAGCACATCATGCAATTGAACAAGGGGCCGCTG 
GACAACGAAGAGGTCGCGCGGCTGTTCCGCGAGATCATGTCGTCCTGCCTGGCGCTCGAGCAGCCGCTGAAA 
GTGGCCTATCTCGGACCCGAGGGCACCTTTACCCAGGCTGCGGCACTCAAGCATTTCGGCAACGCGGTGATC 
AGCACGCCGATGGCGGCGATCGACGAGGTGTTCCGCGAGGTCGCCGCCGGCGCGGTGAACTTCGGCGTGGTG 
CCTGTGGAAAACTCCACCGAGGGGGCGGTCAACCATACCCTCGACAGCTTCCTCGAGCACGACATGGTGATC 
TGCGGCGAAGTCGAGCTGCGTATCCACCATCACCTGCTGGTCGGCGAAACCACCAAGACCGACAACATCACC 
CGGATCTATTCCCATGCCCAATCCCTCGCGCAATGTCGCAAGTGGCTGGACTCGCACTATCCGAGCGTCGAG 
CGGGTGGCGGTGTCGAGCAACGCCGACGCGGCCAAGCGGGTGAAGAGCGAGTGGAATTCGGCAGCGATCGCC 
GGTGACATGGCGGCCAGCCTGTACGACCTGAGCAAGCTGCACGAGAAGATCGAGGACCGCCCGGACAACTCC 
ACGCGCTTCCTGATCATCGGCAACCAGGAGGTTCCGCCGACCGGCGACGACAAGACCTCGATCATCGTTTCC 
ATGCGCAACAAGCCGGGAGCCCTGCACGAGCTGCTGGTACCGTTCCACAACAACGGTATCGACCTGACCCGC 
ATCGAAACCCGTCCGTCGCGCAGCGGCAAATGGACCTACGTGTTCTTCATCGATTTCGTCGGCCACCATAAA 
GAACCGCTGATCAAGGACGTCCTGGAGAAGATCGGCCAGGAAGCCGTCGCCCTGAAGGTCCTGGGCTCCTAT 
CCGAAAGCGGTGCTCTGA