ViewGene
Locus Name:
GeneID:
Location:

GeneID:1768
TrivialName: PA14_19920
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_19920
PAO1 Ortholog Locus:PA3415
Sequence Length:1113
Protein Length:370
Start: 1716776
Stop: 1717888
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06803.1|AE004762_9 (AE004762) probable dihydrolipoamide acetyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 369/370 (99%), Positives = 369/370 (99%)
Sequence:
ATGAAGCACTTCAAGCTGCCCGACCTGGGCGAGGGCCTGCAGGAAGCGGAAATCGTCGAATGGCACGTCAAG
GCCGGCGACAGCGTGCGCGCCGACCAGCGCCTGGTCTCGGTGGAAACCGCCAAGGCGCTGGTGGACATCCCT
GCGCCCTACGATGGGGTGGTGGGCAAGCTGTTCGGCGCCGAGGGCGACATCCTCCACGTCGGCGAACCGCTG
GTGGGCTTCGAGGGCGAGGAGGCGGACGCCGGTACCGTGGTGGGGCGCCTGGAGGGCGGCGGTAGCGCCCTG
GAAGACCGTTTCTTCATCGGCGCCGCACCGTCCACCCGCGAACACCTGGCACCGCGCGCGACCCCGGCGGTG
CGCCAGTTCGCCCGCCAGCAGGGCGTCGAACTGGCCGGCCTGAGCGGCTCCGGCCCGGATGGCCTGATCACC
CGCGCCGACGTCGAGGCCGCCGCCCAGGGCGCCCGCGAACGCTTCGGCGGCGAGCGCTTGCGCGGGGTACGG
CGGAGCATGGCGCTGAACATGGCGCGCTCGCATGCCGAGGTGGTGCCGGTGACGATCTACGGCGACGCCGAC
CTGCATCGCTGGAAGACCGCACGCGACCCGTTGATCCGCCTGGCCCAGGCGCTGGCCGAGGCCTGTCGCGCC
GAGCCGACCCTCAACGCCTGGTTCGATGGCGCCTCGTTGTCGCTGAAACTGCATGAGCGGCTCGACCTGGGG
ATCGCCGTGGACACCCCGGACGGCCTGTTCGTGCCGGTGCTGCGCGACGTCGGTGCGCGTTCGGCGGAGGAC
CTGCGCGCCGGTATGCGGCGCCTGCGCGAAGACGTCCAGGCACGCTCGATCCCGCCGGCGGAAATGCTCGGC
GCGACCCTGACCCTATCCAACTTCGGCACCCTGTTCGGCCGCTACGCCAACCCCGTGGTGGTGCCGCCGCAG
GTGGCCATCCTCGGCGCCGGCGGCATCCGCGACGAAGTAGTGGCCTGGCGCGGCGAGATGGCGATCCACCCG
ATCCTGCCGCTCTCGCTGAGCTTCGATCACCGCGCCGCCACCGGTGGCGAGGCGGCGCGTTTCCTCAAGGTA
CTGGTGAACGCCCTGGAGCAGCCCGACGGCTGA
Translation:
MKHFKLPDLGEGLQEAEIVEWHVKAGDSVRADQRLVSVETAKALVDIPAPYDGVVGKLFGAEGDILHVGEPL
VGFEGEEADAGTVVGRLEGGGSALEDRFFIGAAPSTREHLAPRATPAVRQFARQQGVELAGLSGSGPDGLIT
RADVEAAAQGARERFGGERLRGVRRSMALNMARSHAEVVPVTIYGDADLHRWKTARDPLIRLAQALAEACRA
EPTLNAWFDGASLSLKLHERLDLGIAVDTPDGLFVPVLRDVGARSAEDLRAGMRRLREDVQARSIPPAEMLG
ATLTLSNFGTLFGRYANPVVVPPQVAILGAGGIRDEVVAWRGEMAIHPILPLSLSFDHRAATGGEAARFLKV
LVNALEQPDG*
AnnotationID:1777GeneID:1768
AnnotatorUID: diggins
Modification Date/Time: 2005-04-18 12:12:23
Gene Name:
Confidence Code:3
GeneProduct:putative dihydrolipoamide acetyltransferase
Cell Localization:()
Synonyms:PA3415
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:putative yruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase component
Functional Category Confidence Code:5
COGs:pfam00198,COG0508
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD15501.1| (AL646066) PUTATIVE DIHYDROLIPOAMIDE ACETYLTRANSFERASE (COMPONENT
           E2 OF PYRUVATE DEHYDROGENASE COMPLEX) PROTEIN [Ralstonia
           solanacearum]
          Length = 372

 Score =  402 bits (1034), Expect = e-111
 Identities = 214/366 (58%), Positives = 262/366 (71%), Gaps = 3/366 (0%)

>gb|AAO90182.1| (AE016961) dehydrogenase, E2 component, acyltransferase [Coxiella
           burnetii RSA 493]
          Length = 378

 Score =  338 bits (867), Expect = 3e-92
 Identities = 183/378 (48%), Positives = 248/378 (65%), Gaps = 13/378 (3%)








 
Structural Features:
pfam00198, 2-oxoacid_dh, 2-oxoacid dehydrogenases acyltransferase 
(catalytic domain).
CD-Length = 232 residues,  95.7% aligned
Score =  214 bits (548), Expect = 1e-56
Identities = 76/222 (34%), Positives = 120/222 (54%), Gaps = 14/222 (6%)

COG0508, AceF, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dihydrolipoamide acyltransferase (E2) component, and related enzymes 
[Energy production and conversion]
CD-Length = 404 residues,  98.3% aligned
Score =  265 bits (678), Expect = 7e-72
Identities = 135/399 (33%), Positives = 196/399 (49%), Gaps = 33/399 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3539




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20686




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32151




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1768_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1768
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3132
                           PA3415_tr
                           translation of PA3415
                           370
                           99.72
                           99.72
                           0
                           1879.0
     

     
     
97046
                           gb|AAG06803.1|AE004762_9
                           (AE004762) probable dihydrolipoamide acetyltransferase [Pseudomonas aeruginosa PAO1]
                           370
                           99.72
                           99.72
                           0
                           1879.0
     

     
     
97047
                           emb|CAD15501.1|
                           (AL646066) PUTATIVE DIHYDROLIPOAMIDE ACETYLTRANSFERASE (COMPONENT E2 OF PYRUVATE DEHYDROGENASE COMPLEX) PROTEIN [Ralstonia solanacearum]
                           374
                           58.02
                           70.58
                           6
                           1034.0
     

     
     
97048
                           gb|AAO90182.1|
                           (AE016961) dehydrogenase, E2 component, acyltransferase [Coxiella burnetii RSA 493]
                           384
                           48.17
                           65.10
                           20
                           880.0
     

     
     
97049
                           dbj|BAC61988.1|
                           (AP005086) putative dihydrolipoamide acetyltransferase [Vibrio parahaemolyticus]
                           390
                           44.35
                           59.23
                           24
                           775.0
     

     






      

          
GC ORFID: 44770How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3415Glimmer Score: 

          
Start: 3237156Stop: 3236044

          
Length: 1113


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3415 translation of PA3415Homolog Bit Score 728.0

          
Other Homologs:  t_PA3415 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCACTTCAAGCTGCCCGACCTGGGCGAGGGCCTGCAGGAAGCGGAAATCGTCGAATGGCACGTCAAG 
GCCGGCGACAGCGTGCGCGCCGACCAGCGCCTGGTCTCGGTGGAAACCGCCAAGGCGCTGGTGGACATCCCT 
GCGCCCTACGATGGGGTGGTGGGCAAGCTGTTCGGCGCCGAGGGCGACATCCTCCACGTCGGCGAACCGCTG 
GTGGGCTTCGAGGGCGAGGAGGCGGACGCCGGTACCGTGGTGGGGCGCCTGGAGGGCGGCGGTAGCGCCCTG 
GAAGACCGTTTCTTCATCGGCGCCGCACCGTCCACCCGCGAACACCTGGCACCGCGCGCGACCCCGGCGGTG 
CGCCAGTTCGCCCGCCAGCAGGGCGTCGAACTGGCCGGCCTGAGCGGCTCCGGCCCGGATGGCCTGATCACC 
CGCGCCGACGTCGAGGCCGCCGCCCAGGGCGCCCGCGAACGCTTCGGCGGCGAGCGCTTGCGCGGGGTACGG 
CGGAGCATGGCGCTGAACATGGCGCGCTCGCATGCCGAGGTGGTGCCGGTGACGATCTACGGCGACGCCGAC 
CTGCATCGCTGGAAGACCGCACGCGACCCGTTGATCCGCCTGGCCCAGGCGCTGGCCGAGGCCTGTCGCGCC 
GAGCCGACCCTCAACGCCTGGTTCGATGGCGCCTCGTTGTCGCTGAAACTGCATGAGCGGCTCGACCTGGGG 
ATCGCCGTGGACACCCCGGACGGCCTGTTCGTGCCGGTGCTGCGCGACGTCGGTGCGCGTTCGGCGGAGGAC 
CTGCGCGCCGGTATGCGGCGCCTGCGCGAAGACGTCCAGGCACGCTCGATCCCGCCGGCGGAAATGCTCGGC 
GCGACCCTGACCCTATCCAACTTCGGCACCCTGTTCGGCCGCTACGCCAACCCCGTGGTGGTGCCGCCGCAG 
GTGGCCATCCTCGGCGCCGGCGGCATCCGCGACGAAGTAGTGGCCTGGCGCGGCGAGATGGCGATCCACCCG 
ATCCTGCCGCTCTCGCTGAGCTTCGATCACCGCGCCGCCACCGGTGGCGAGGCGGCGCGTTTCCTCAAGGTA 
CTGGTGAACGCCCTGGAGCAGCCCGACGGCTGA