ViewGene
Locus Name:
GeneID:
Location:

GeneID:1766
TrivialName: PA14_66240
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66240
PAO1 Ortholog Locus:PA5011
Sequence Length:1065
Protein Length:354
Start: 5902250
Stop: 5901186
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08396.1|AE004913_11 (AE004913) heptosyltransferase I [Pseudomonas aeruginosa PAO1]
Identities = 354/354 (100%), Positives = 354/354 (100%)
Sequence:
ATGAGGGTACTGCTGGTCAAGACCTCGTCCCTCGGCGACGTGATCCACACCCTGCCGGCGCTTACCGACGCC
GCCCGGGCGATTCCCGGCATCCAGTTCGACTGGGTGGTGGAGGAAGGTTTCGCCGAGATTCCCGCCTGGCAT
CCGGCGGTGGCGCGGGTGATCCCGGTGGCGATCCGGCGCTGGCGCAAGAATCTCTGGCAGACCCTGCGCAAC
GGCGAATGGCGGCGCTTCAAACAGCGCCTGAAGGAAGTCGACTATGACCTGGTGATCGACGCCCAGGGGCTG
CTGAAGAGTGCCTGGCTGACCCGCTACGTGGGCAAGACGCCGGTCGCCGGTCTCGATCGCGATTCGGCGCGC
GAGCCGCTCGCCAGCCGCTTCTATCGCCGTGCCTATCCGGTCGCCTGGGGACAGCATGCGGTGGAGCGTACG
CGCCAGTTGTTCGCCCAGGCGCTGGACTACCCGTTGCCCGAGTCGGTCGGTGACTATGGCCTGGACCGCGAG
CAGTTGGCCGACGCCGACCCTGGAGCGCCGTACCTGGTGTTCCTGCACGGTACTACCTGGGTCACCAAGCAT
TGGCCGGAAGCCTACTGGCGCGAACTGGCCGAGCGCATGTGCGAGCGCGGCTGGTCGGTGCGCCTGCCCTGG
GGCAGCGCCGCCGAGCGGGAGCGGGCCGGGCGCCTGGCGGCGGGGTTGGAAAATGCCGCGGTACTCCCCAGA
TTATCCCTGGCCGGCATGGCCAAGGTGCTTGCCGGCGCGCGCGCCTGCGTGGCGGTGGATACCGGCCTCGGT
CACCTGGCGGCGGCACTGGACGTGCCGACGCTGTCGCTGTTCGGCCCGACCAATCCTGGCTTCACCGGCGCC
TACGGGCGCTCCCAGGTCCACCTGGGCAGCGACTTCCCCTGTGCGCCGTGCCTGAAGAAGACCTGTACCTAC
CAGCCGACCGAAGAGGACCGCAAACTGTTCGATCTCAAGCGTGAGCAGCCGCTGTGCTTCACCCGGCTGAAT
CCCCAGCGCGTGGCCACCCAGCTGGAGGCCATGCTGCTGGCCCCGGAGACCCTCTGA
Translation:
MRVLLVKTSSLGDVIHTLPALTDAARAIPGIQFDWVVEEGFAEIPAWHPAVARVIPVAIRRWRKNLWQTLRN
GEWRRFKQRLKEVDYDLVIDAQGLLKSAWLTRYVGKTPVAGLDRDSAREPLASRFYRRAYPVAWGQHAVERT
RQLFAQALDYPLPESVGDYGLDREQLADADPGAPYLVFLHGTTWVTKHWPEAYWRELAERMCERGWSVRLPW
GSAAERERAGRLAAGLENAAVLPRLSLAGMAKVLAGARACVAVDTGLGHLAAALDVPTLSLFGPTNPGFTGA
YGRSQVHLGSDFPCAPCLKKTCTYQPTEEDRKLFDLKREQPLCFTRLNPQRVATQLEAMLLAPETL*
AnnotationID:1775GeneID:1766
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 09:09:49
Gene Name:waaC
Confidence Code:1
GeneProduct:lipopolysaccharide heptosyltransferase I
Cell Localization:()
Synonyms:rfaC
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0859,pfam01075
Secondary Functional Category(ies):
EC Number:2.-.-.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58430.1| (AE016874) lipopolysaccharide heptosyltransferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 354

 Score =  546 bits (1406), Expect = e-155
 Identities = 266/353 (75%), Positives = 301/353 (85%), Gaps = 2/353 (0%)

>gb|AAN65973.1|AE016226_2 (AE016775) heptosyltransferase I [Pseudomonas putida KT2440]
          Length = 352

 Score =  541 bits (1395), Expect = e-153
 Identities = 263/352 (74%), Positives = 300/352 (85%), Gaps = 2/352 (0%)

>emb|CAB63262.1| (AJ132396) heptosyltransferase I [Klebsiella pneumoniae]
          Length = 323

 Score =  337 bits (863), Expect = 8e-92
 Identities = 170/294 (57%), Positives = 214/294 (72%), Gaps = 1/294 (0%)








 
Structural Features:
COG0859, RfaF, ADP-heptose:LPS heptosyltransferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 334 residues,  94.3% aligned
Score =  258 bits (661), Expect = 6e-70
Identities = 115/323 (35%), Positives = 165/323 (51%), Gaps = 14/323 (4%)

pfam01075, Glyco_transf_9, Glycosyltransferase family 9 (heptosyltransferase). 
CD-Length = 248 residues, 100.0% aligned
Score =  254 bits (649), Expect = 2e-68
Identities = 109/250 (43%), Positives = 131/250 (52%), Gaps = 10/250 (4%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3535




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20682




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35055




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1766_cdd.html


    		




 
URL:

















    ReferenceID:35871




  
Author/Investigator(s):
        de Kievit TR, Lam JS.





  
Title:
        Isolation and characterization of two genes, waaC (rfaC) and waaF (rfaF), involved in Pseudomonas aeruginosa serotype O5 inner-core biosynthesis.




  
PubMed:
        9171387


MedLine:
        




    
Source:
J Bacteriol. 1997 Jun;179(11):3451-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9171387









           
Homologs By Global Alignment 

           

       



     
Gene ID:1766
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3130
                           PA5011_tr
                           translation of PA5011
                           355
                           99.71
                           99.71
                           1
                           1881.0
     

     
     
96978
                           gb|AAG08396.1|AE004913_11
                           (AE004913) heptosyltransferase I [Pseudomonas aeruginosa PAO1]
                           355
                           99.71
                           99.71
                           1
                           1881.0
     

     
     
96979
                           gb|AAC45365.1|
                           (U70982) heptosyl transferase I [Pseudomonas aeruginosa]
                           355
                           99.43
                           99.71
                           1
                           1877.0
     

     
     
96980
                           gb|AAC33167.1|
                           (U63816) lipopolysaccharide heptosyltransferase I homolog [Pseudomonas aeruginosa]
                           355
                           99.43
                           99.43
                           1
                           1874.0
     

     
     
96981
                           gb|AAO58430.1|
                           (AE016874) lipopolysaccharide heptosyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           355
                           74.92
                           84.78
                           2
                           1405.0
     

     






      

          
GC ORFID: 44768How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5011Glimmer Score: 

          
Start: 5165933Stop: 5164869

          
Length: 1065


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5011 translation of PA5011Homolog Bit Score 729.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3379 (ORF: BLASTX 546.0), t_PA5011 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGGGTACTGCTGGTCAAGACCTCGTCCCTCGGCGACGTGATCCACACCCTGCCGGCGCTTACCGACGCC 
GCCCGGGCGATTCCCGGCATCCAGTTCGACTGGGTGGTGGAGGAAGGTTTCGCCGAGATTCCCGCCTGGCAT 
CCGGCGGTGGCGCGGGTGATCCCGGTGGCGATCCGGCGCTGGCGCAAGAATCTCTGGCAGACCCTGCGCAAC 
GGCGAATGGCGGCGCTTCAAACAGCGCCTGAAGGAAGTCGACTATGACCTGGTGATCGACGCCCAGGGGCTG 
CTGAAGAGTGCCTGGCTGACCCGCTACGTGGGCAAGACGCCGGTCGCCGGTCTCGATCGCGATTCGGCGCGC 
GAGCCGCTCGCCAGCCGCTTCTATCGCCGTGCCTATCCGGTCGCCTGGGGACAGCATGCGGTGGAGCGTACG 
CGCCAGTTGTTCGCCCAGGCGCTGGACTACCCGTTGCCCGAGTCGGTCGGTGACTATGGCCTGGACCGCGAG 
CAGTTGGCCGACGCCGACCCTGGAGCGCCGTACCTGGTGTTCCTGCACGGTACTACCTGGGTCACCAAGCAT 
TGGCCGGAAGCCTACTGGCGCGAACTGGCCGAGCGCATGTGCGAGCGCGGCTGGTCGGTGCGCCTGCCCTGG 
GGCAGCGCCGCCGAGCGGGAGCGGGCCGGGCGCCTGGCGGCGGGGTTGGAAAATGCCGCGGTACTCCCCAGA 
TTATCCCTGGCCGGCATGGCCAAGGTGCTTGCCGGCGCGCGCGCCTGCGTGGCGGTGGATACCGGCCTCGGT 
CACCTGGCGGCGGCACTGGACGTGCCGACGCTGTCGCTGTTCGGCCCGACCAATCCTGGCTTCACCGGCGCC 
TACGGGCGCTCCCAGGTCCACCTGGGCAGCGACTTCCCCTGTGCGCCGTGCCTGAAGAAGACCTGTACCTAC 
CAGCCGACCGAAGAGGACCGCAAACTGTTCGATCTCAAGCGTGAGCAGCCGCTGTGCTTCACCCGGCTGAAT 
CCCCAGCGCGTGGCCACCCAGCTGGAGGCCATGCTGCTGGCCCCGGAGACCCTCTGA