ViewGene
Locus Name:
GeneID:
Location:

GeneID:1765
TrivialName: PA14_17220
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17220
PAO1 Ortholog Locus:PA3643
Sequence Length:1137
Protein Length:378
Start: 1475829
Stop: 1476965
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07031.1|AE004784_4 (AE004784) lipid A-disaccharide synthase [Pseudomonas aeruginosa PAO1]
Identities = 377/378 (99%), Positives = 378/378 (100%)
Sequence:
ATGGCTGACGGATTGCGCGTAGCCCTGGTCGCCGGCGAGGCCTCCGGCGATATCCTCGGGTCCGGACTGATG
CAGGCGCTGCGGGCGCGGCACCCGGATATCGAGTTCATCGGCGTCGGTGGGCCGCGCATGGAGGCCGAAGGG
CTGTCCAGCTATTTCCCCATGGAGCGCCTGTCGGTGATGGGTCTGGTCGAAGTACTGGGCCGGCTGCCGGAG
TTGTTGCGACGGCGCAAGCGCCTGATCCGCACGCTCATCGAGGCCCGGCCGGATGTGATGATCGGCATCGAC
GCTCCCGACTTCACCCTCGGCGTCGAACACAAGCTGCGCCAGGCTGGCCTGCGCACCGTGCATTACGTCAGC
CCTTCGGTGTGGGCCTGGCGGCAGAAGCGGGTGCTGAAGATTCGCGAGGCCTGCGACCTGATGCTGGCATTG
TTCCCCTTCGAAGCACGTTTCTACGAAGAGCATGGGGTGCCCGTGCGTTTCGTCGGCCACCCCCTGGCCAAC
ACCATTCCCCTGCAGGCGGATCGCGCTGCGGCCCGCGCGCGGCTCGGATTGCCGGCGGACGGACAGGTGGTG
GCGCTGATGCCGGGCAGTCGCGGCGGCGAGGTCGGCAAGCTCGGCGCCTTGTTCCTCGACACCGCCCAGCGC
CTGCTCGTCGAACGGCCGGGCCTGCGTTTCGTGCTGCCCTGCGCCAGCGCTGCACGGCGCGAACAGATCGAG
CAGATGCTCCAGGGCCGCGAGCCTCTGCCGCTGACGCTGCTCGACGGCGCTTCCCATGAAGCGTTGGCCGCC
TGCGATGCGGTGCTGATCGCCTCCGGTACCGCGACCCTCGAGGCGCTGCTCTACAAGCGGCCCATGGTGGTG
GCTTATCGGGTCGCCGGGCTGACGTACCGGATTCTCAAGCGCCTGGTGAAAAGCCCTTACATCTCCCTGCCG
AACCTGTTGGCCGGACGCCTGTTGGTGCCGGAACTGATCCAGGACGCCGCCACCCCGCAAGCGCTGGCCGCA
ACCCTCTCGCCGCTGCTGGACGACGGCTCGCAGCAGGTGGAATTCTTCGATGCCATCCATCGGGCCTTGCGC
CAGGATGCTTCGGCACAGGCGGCGGAAGCCGTCCTGCAACTGGTGGAGCGCCGCTGA
Translation:
MADGLRVALVAGEASGDILGSGLMQALRARHPDIEFIGVGGPRMEAEGLSSYFPMERLSVMGLVEVLGRLPE
LLRRRKRLIRTLIEARPDVMIGIDAPDFTLGVEHKLRQAGLRTVHYVSPSVWAWRQKRVLKIREACDLMLAL
FPFEARFYEEHGVPVRFVGHPLANTIPLQADRAAARARLGLPADGQVVALMPGSRGGEVGKLGALFLDTAQR
LLVERPGLRFVLPCASAARREQIEQMLQGREPLPLTLLDGASHEALAACDAVLIASGTATLEALLYKRPMVV
AYRVAGLTYRILKRLVKSPYISLPNLLAGRLLVPELIQDAATPQALAATLSPLLDDGSQQVEFFDAIHRALR
QDASAQAAEAVLQLVERR*
AnnotationID:1774GeneID:1765
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 21:09:00
Gene Name:lpxB
Confidence Code:2
GeneProduct: lipid A-disaccharide synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:tetraacyldisaccharide-1-P
Functional Category Confidence Code:5
COGs:COG0763,pfam02684
Secondary Functional Category(ies):
EC Number:2.4.1.182
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67225.1|AE016349_6 (AE016779) lipid A disaccharide synthase [Pseudomonas putida
           KT2440]
          Length = 375

 Score =  576 bits (1484), Expect = e-164
 Identities = 301/374 (80%), Positives = 332/374 (88%), Gaps = 2/374 (0%)

>gb|AAP15715.1| (AE016978) tetraacyldisaccharide-1-P [Shigella flexneri 2a str.
           2457T]
          Length = 382

 Score =  377 bits (968), Expect = e-104
 Identities = 200/376 (53%), Positives = 258/376 (68%), Gaps = 4/376 (1%)

>gb|AAC73293.1| (AE000127) tetraacyldisaccharide-1-P; lipid A biosynthesis,
           penultimate step [Escherichia coli K12]
          Length = 382

 Score =  377 bits (967), Expect = e-104
 Identities = 200/376 (53%), Positives = 258/376 (68%), Gaps = 4/376 (1%)







 
Structural Features:
COG0763, LpxB, Lipid A disaccharide synthetase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 381 residues,  99.7% aligned
Score =  464 bits (1195), Expect = 9e-132
Identities = 209/380 (55%), Positives = 269/380 (70%), Gaps = 7/380 (1%)

pfam02684, LpxB, Lipid-A-disaccharide synthetase
CD-Length = 373 residues, 100.0% aligned
Score =  370 bits (950), Expect = 2e-103
Identities = 167/374 (44%), Positives = 230/374 (61%), Gaps = 10/374 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3533




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20680




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31900




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1765_cdd.html


    		




 
URL:

















    ReferenceID:34206




  
Author/Investigator(s):
        Foster SJ.





  
Title:
        Purification and characterization of an 'actomyosin' complex from Escherichia coli W3110.




  
PubMed:
        8354462


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1993 Jul 1;110(3):295-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8354462

















    ReferenceID:34207




  
Author/Investigator(s):
        Crowell DN, Reznikoff WS, Raetz CR.





  
Title:
        Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase.




  
PubMed:
        2824445


MedLine:
        




    
Source:
J Bacteriol. 1987 Dec;169(12):5727-34.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2824445

















    ReferenceID:34208




  
Author/Investigator(s):
        Crowell DN, Anderson MS, Raetz CR.





  
Title:
        Molecular cloning of the genes for lipid A disaccharide synthase and UDP-N-acetylglucosamine acyltransferase in Escherichia coli.




  
PubMed:
        3531165


MedLine:
        




    
Source:
J Bacteriol. 1986 Oct;168(1):152-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3531165

















    ReferenceID:34209




  
Author/Investigator(s):
        Tomasiewicz HG, McHenry CS.





  
Title:
        Sequence analysis of the Escherichia coli dnaE gene.




  
PubMed:
        3316192


MedLine:
        




    
Source:
J Bacteriol. 1987 Dec;169(12):5735-44.

Erratum in:
    * J Bacteriol 1991 Jul;173(14):4549.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3316192









           
Homologs By Global Alignment 

           

       



     
Gene ID:1765
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3129
                           PA3643_tr
                           translation of PA3643
                           378
                           99.73
                           99.99
                           0
                           1883.0
     

     
     
96938
                           gb|AAG07031.1|AE004784_4
                           (AE004784) lipid A-disaccharide synthase [Pseudomonas aeruginosa PAO1]
                           378
                           99.73
                           99.99
                           0
                           1883.0
     

     
     
96940
                           gb|AAN67225.1|AE016349_6
                           (AE016779) lipid A disaccharide synthase [Pseudomonas putida KT2440]
                           378
                           80.15
                           88.35
                           3
                           1487.0
     

     
     
96939
                           gb|AAO55067.1|
                           (AE016861) lipid A disaccharide synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           381
                           77.95
                           86.87
                           4
                           1501.0
     

     
     
96945
                           gb|AAP15715.1|
                           (AE016978) tetraacyldisaccharide-1-P [Shigella flexneri 2a str. 2457T]
                           384
                           52.60
                           67.70
                           8
                           971.0
     

     






      

          
GC ORFID: 44767How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3643Glimmer Score: 

          
Start: 3478108Stop: 3476972

          
Length: 1137


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3643 translation of PA3643Homolog Bit Score 729.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4632 (ORF: BLASTX 563.0), t_PA3643 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTGACGGATTGCGCGTAGCCCTGGTCGCCGGCGAGGCCTCCGGCGATATCCTCGGGTCCGGACTGATG 
CAGGCGCTGCGGGCGCGGCACCCGGATATCGAGTTCATCGGCGTCGGTGGGCCGCGCATGGAGGCCGAAGGG 
CTGTCCAGCTATTTCCCCATGGAGCGCCTGTCGGTGATGGGTCTGGTCGAAGTACTGGGCCGGCTGCCGGAG 
TTGTTGCGACGGCGCAAGCGCCTGATCCGCACGCTCATCGAGGCCCGGCCGGATGTGATGATCGGCATCGAC 
GCTCCCGACTTCACCCTCGGCGTCGAACACAAGCTGCGCCAGGCTGGCCTGCGCACCGTGCATTACGTCAGC 
CCTTCGGTGTGGGCCTGGCGGCAGAAGCGGGTGCTGAAGATTCGCGAGGCCTGCGACCTGATGCTGGCATTG 
TTCCCCTTCGAAGCACGTTTCTACGAAGAGCATGGGGTGCCCGTGCGTTTCGTCGGCCACCCCCTGGCCAAC 
ACCATTCCCCTGCAGGCGGATCGCGCTGCGGCCCGCGCGCGGCTCGGATTGCCGGCGGACGGACAGGTGGTG 
GCGCTGATGCCGGGCAGTCGCGGCGGCGAGGTCGGCAAGCTCGGCGCCTTGTTCCTCGACACCGCCCAGCGC 
CTGCTCGTCGAACGGCCGGGCCTGCGTTTCGTGCTGCCCTGCGCCAGCGCTGCACGGCGCGAACAGATCGAG 
CAGATGCTCCAGGGCCGCGAGCCTCTGCCGCTGACGCTGCTCGACGGCGCTTCCCATGAAGCGTTGGCCGCC 
TGCGATGCGGTGCTGATCGCCTCCGGTACCGCGACCCTCGAGGCGCTGCTCTACAAGCGGCCCATGGTGGTG 
GCTTATCGGGTCGCCGGGCTGACGTACCGGATTCTCAAGCGCCTGGTGAAAAGCCCTTACATCTCCCTGCCG 
AACCTGTTGGCCGGACGCCTGTTGGTGCCGGAACTGATCCAGGACGCCGCCACCCCGCAAGCGCTGGCCGCA 
ACCCTCTCGCCGCTGCTGGACGACGGCTCGCAGCAGGTGGAATTCTTCGATGCCATCCATCGGGCCTTGCGC 
CAGGATGCTTCGGCACAGGCGGCGGAAGCCGTCCTGCAACTGGTGGAGCGCCGCTGA