ViewGene
Locus Name:
GeneID:
Location:

GeneID:1764
TrivialName: PA14_26130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_26130
PAO1 Ortholog Locus:PA2932
Sequence Length:1110
Protein Length:369
Start: 2282021
Stop: 2280912
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06320.1|AE004719_6 (AE004719) morphinone reductase [Pseudomonas aeruginosa PAO1]
Identities = 368/369 (99%), Positives = 368/369 (99%)
Sequence:
ATGTCCAACCTGCTCCTCTCCCCGCTCGCCGTAGGCAACCTGGCCTTGCGCAACCGCATCGTCATGGCGCCG
ATGACCCGCTCCCGGGCGCAACAGCCGGGCGACGTGCCGACCGCCCTGAACGCTCTCTACTATGCGCAGCGC
GCCGGCGCCGGGCTGATCGTCAGCGAGGGCACGCAGATTTCCCGCCTCGGCCAGGGCTACGCCTACACCCCC
GGTATCTACAGCGAGGCGCAACTGGCCGGCTGGCGCCAGGTCACCGAGGCGGTGCACGCCGCCGGCGGACTG
ATCGCCGCGCAGCTCTGGCATGTCGGGCGCATGTCGCATCGCTCGCTGCAGGCCGGCGGCGAGGCGCCGATC
GCGCCCTCGCCGATCCAGGCCAAGGCCCAGGTGTTCATCGCCGATGGCCAGGGCGGCGGATCCATGGCGCCG
GCCGACGCGCCGCGGGAAATGACCCTGGAAGACATCCGCCGGGTCCGCGACGAATTCGTCCGCGCCGCGCGC
AACGCGCTCGACGCCGGCTTCGACCTGGTCGAGCTGCATGGCGCCAATGGCTACCTGATCGACCAGTTCCTC
GCCAGCGCCAGCAACCGGCGCAGCGATGCCTATGGCGGTAGCCTGGAGAACCGCGCGCGGTTCCTGCTGGAG
ATCGTCGACGCGCTGGTCGCCGCGGTCGGTGCCGAGCGCGTCGGCCTGCGCCTGTCGCCCTGGGGCACGATC
AACGACATGCACGACGACGAGCCGGAAGCGATGACCCTTTACCTCGCCGAAGCCCTGCAGCGACGCGGCATC
GCCTACCTGCACCTGGCCGAGTGGGAATGGAGCGGCGGCCCGGCCTACCCGCAAGGCTTCCGCGAGCGCCTG
CGCGAACGCTTCCGCGCGCCGCTGATCGTCTGCGGCAACTACGACGCCGAGCGCGCCGAAGCGATACTCCAG
GCCGGACTGGCCGACGCGGTGGCGATCGGCCGGCCGTTCATCGCCAACCCCGACCTGGTCGAGCGGATCCGT
CTCGGCGCGCCGCTGGCCGAGGCCAACCAGGCGCGGTTCTACGGTGGCGACGCCGCCGGCTACACCGATTAC
CCGACCCTCGGCCAGTCCGCTACGGCCTGA
Translation:
MSNLLLSPLAVGNLALRNRIVMAPMTRSRAQQPGDVPTALNALYYAQRAGAGLIVSEGTQISRLGQGYAYTP
GIYSEAQLAGWRQVTEAVHAAGGLIAAQLWHVGRMSHRSLQAGGEAPIAPSPIQAKAQVFIADGQGGGSMAP
ADAPREMTLEDIRRVRDEFVRAARNALDAGFDLVELHGANGYLIDQFLASASNRRSDAYGGSLENRARFLLE
IVDALVAAVGAERVGLRLSPWGTINDMHDDEPEAMTLYLAEALQRRGIAYLHLAEWEWSGGPAYPQGFRERL
RERFRAPLIVCGNYDAERAEAILQAGLADAVAIGRPFIANPDLVERIRLGAPLAEANQARFYGGDAAGYTDY
PTLGQSATA*
AnnotationID:1773GeneID:1764
AnnotatorUID: diggins
Modification Date/Time: 2005-04-08 10:10:31
Gene Name:morB
Confidence Code:2
GeneProduct:morphinone reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:NADH:flavin oxidoreductase
Functional Category Confidence Code:5
COGs:COG1902,pfam00724
Secondary Functional Category(ies):energy metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD14904.1| (AL646063) PROBABLE FLAVOPROTEIN NADH-DEPENDENT OXIDOREDUCTASE
           [Ralstonia solanacearum]
          Length = 378

 Score =  433 bits (1114), Expect = e-121
 Identities = 215/368 (58%), Positives = 269/368 (73%), Gaps = 1/368 (0%)

>gb|AAC43569.1| (U37350) morphinone reductase [Pseudomonas putida]
          Length = 377

 Score =  394 bits (1012), Expect = e-109
 Identities = 199/361 (55%), Positives = 256/361 (70%), Gaps = 4/361 (1%)

>dbj|BAC61728.1| (AP005085) N-ethylmaleimide reductase [Vibrio parahaemolyticus]
          Length = 374

 Score =  390 bits (1002), Expect = e-108
 Identities = 198/364 (54%), Positives = 255/364 (70%), Gaps = 2/364 (0%)






 
Structural Features:
COG1902, NemA, NADH:flavin oxidoreductases, Old Yellow Enzyme family 
[Energy production and conversion]
CD-Length = 363 residues,  99.4% aligned
Score =  346 bits (890), Expect = 2e-96
Identities = 171/380 (45%), Positives = 225/380 (59%), Gaps = 32/380 (8%)

pfam00724, Oxidored_FMN, NADH:flavin oxidoreductase / NADH oxidase family.
CD-Length = 335 residues,  99.4% aligned
Score =  282 bits (723), Expect = 5e-77
Identities = 143/350 (40%), Positives = 200/350 (57%), Gaps = 30/350 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3531




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20678




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36116




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1764_cdd.html


    		




 
URL:

















    ReferenceID:36420




  
Author/Investigator(s):
        Trigui M, Pulvin S, Truffaut N, Thomas D, Poupin P.





  
Title:
        Molecular cloning, nucleotide sequencing and expression of genes encoding a cytochrome P450 system involved in secondary amine utilization in Mycobacterium sp. strain RP1.




  
PubMed:
        14759702


MedLine:
        




    
Source:
Res Microbiol. 2004 Jan-Feb;155(1):1-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14759702

















    ReferenceID:36421




  
Author/Investigator(s):
        French CE, Bruce NC.





  
Title:
        Bacterial morphinone reductase is related to Old Yellow Enzyme.




  
PubMed:
        8554504


MedLine:
        




    
Source:
Biochem J. 1995 Dec 15;312 ( Pt 3):671-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8554504









           
Homologs By Global Alignment 

           

       



     
Gene ID:1764
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3126
                           PA2932_tr
                           translation of PA2932
                           369
                           99.72
                           99.72
                           0
                           1881.0
     

     
     
96855
                           gb|AAG06320.1|AE004719_6
                           (AE004719) morphinone reductase [Pseudomonas aeruginosa PAO1]
                           369
                           99.72
                           99.72
                           0
                           1881.0
     

     
     
96856
                           emb|CAD14904.1|
                           (AL646063) PROBABLE FLAVOPROTEIN NADH-DEPENDENT OXIDOREDUCTASE [Ralstonia solanacearum]
                           380
                           56.57
                           70.78
                           13
                           1116.0
     

     
     
3127
                           PA1334_tr
                           translation of PA1334
                           375
                           56.00
                           68.80
                           11
                           1036.0
     

     
     
96857
                           gb|AAG04723.1|AE004562_10
                           (AE004562) probable oxidoreductase [Pseudomonas aeruginosa PAO1]
                           375
                           56.00
                           68.80
                           11
                           1036.0
     

     






      

          
GC ORFID: 44766How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2932Glimmer Score: 

          
Start: 2671904Stop: 2673013

          
Length: 1110


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2932 translation of PA2932Homolog Bit Score 729.0

          
Other Homologs:  t_PA1334 (ORF: BLASTX 402.0), psyr_15may02_Scaffold10_revised_gene1546 (ORF: BLASTX 324.0), psyr_15may02_Scaffold7_revised_gene7163 (ORF: BLASTX 315.0), t_PA4356 (ORF: BLASTX 303.0), psyr_15may02_Scaffold12_revised_gene1974 (ORF: BLASTX 293.0), t_PA2932 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCAACCTGCTCCTCTCCCCGCTCGCCGTAGGCAACCTGGCCTTGCGCAACCGCATCGTCATGGCGCCG 
ATGACCCGCTCCCGGGCGCAACAGCCGGGCGACGTGCCGACCGCCCTGAACGCTCTCTACTATGCGCAGCGC 
GCCGGCGCCGGGCTGATCGTCAGCGAGGGCACGCAGATTTCCCGCCTCGGCCAGGGCTACGCCTACACCCCC 
GGTATCTACAGCGAGGCGCAACTGGCCGGCTGGCGCCAGGTCACCGAGGCGGTGCACGCCGCCGGCGGACTG 
ATCGCCGCGCAGCTCTGGCATGTCGGGCGCATGTCGCATCGCTCGCTGCAGGCCGGCGGCGAGGCGCCGATC 
GCGCCCTCGCCGATCCAGGCCAAGGCCCAGGTGTTCATCGCCGATGGCCAGGGCGGCGGATCCATGGCGCCG 
GCCGACGCGCCGCGGGAAATGACCCTGGAAGACATCCGCCGGGTCCGCGACGAATTCGTCCGCGCCGCGCGC 
AACGCGCTCGACGCCGGCTTCGACCTGGTCGAGCTGCATGGCGCCAATGGCTACCTGATCGACCAGTTCCTC 
GCCAGCGCCAGCAACCGGCGCAGCGATGCCTATGGCGGTAGCCTGGAGAACCGCGCGCGGTTCCTGCTGGAG 
ATCGTCGACGCGCTGGTCGCCGCGGTCGGTGCCGAGCGCGTCGGCCTGCGCCTGTCGCCCTGGGGCACGATC 
AACGACATGCACGACGACGAGCCGGAAGCGATGACCCTTTACCTCGCCGAAGCCCTGCAGCGACGCGGCATC 
GCCTACCTGCACCTGGCCGAGTGGGAATGGAGCGGCGGCCCGGCCTACCCGCAAGGCTTCCGCGAGCGCCTG 
CGCGAACGCTTCCGCGCGCCGCTGATCGTCTGCGGCAACTACGACGCCGAGCGCGCCGAAGCGATACTCCAG 
GCCGGACTGGCCGACGCGGTGGCGATCGGCCGGCCGTTCATCGCCAACCCCGACCTGGTCGAGCGGATCCGT 
CTCGGCGCGCCGCTGGCCGAGGCCAACCAGGCGCGGTTCTACGGTGGCGACGCCGCCGGCTACACCGATTAC 
CCGACCCTCGGCCAGTCCGCTACGGCCTGA