ViewGene
Locus Name:
GeneID:
Location:

GeneID:1761
TrivialName: PA14_45720
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45720
PAO1 Ortholog Locus:PA1449
Sequence Length:1137
Protein Length:378
Start: 4067894
Stop: 4066758
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04838.1|AE004574_9 (AE004574) flagellar biosynthetic protein FlhB [Pseudomonas aeruginosa PAO1]
Identities = 378/378 (100%), Positives = 378/378 (100%)
Sequence:
ATGGCCGAGAGCGAGAGCGGGCAGGACAAGACAGAGGAACCCACGGAGAAACGCCGCCGCGAGGCGCGCGAG
AAAGGGCAGTTGCCGCGTTCGCGGGAGCTGAACACCCTGGCCATCCTCATGGCCGGCGCCGGCGGCCTGCTG
ATCTACGGCGCCGACCTGGCGGGCGCCCTGTTGCGCCTGATGCGCAGCAATTTCGAGCTGTCCCGGGAAACC
GCGATGAATACCGAGAGCATGCTGCAATTGCTCGGCGCTTCGGCCTACCTGGCTGCCCAGGGGCTCTGGCCG
ATCCTGCTGATGCTGCTGGTGGCGGCGATCGTCGGGCCGATCGCCCTCGGCGGCTGGCTGTTCTCGATGGAC
GCCCTGCAACCCAAGTTCAGCCGGCTCAACCCGCTGTCGGGGCTGAAGCGGATGTTTTCCGCCAAGTCGCTG
CTCGAACTGAGCAAGGCGCTGATCAAGTTCCTGGTGGTGCTGGCGGTGGCCCTGCTGGTGCTCTCCGCCGAC
CGCGACGCCCTCCTGGCCCTGGCGCACCAGCCGCTCGAGCAGGCGATCCTGCACAGCGTGCGGGTGGTCGGC
TGGAGCGCCTTCTGGATGGCCTGCAGCCTGCTGCTGATCGCCGCGGTGGACGTGCCCTACCAGATCTGGGAC
AACCGCCAGAAGCTGCTGATGACCAAGCAGGAAGTCCGCGACGAATACAAGGACTCCGAGGGCAAGCCCGAG
GTCAAGTCGAAGATCCGCCAGATGCAGCGCGAGATGGCGCAGAGACGGATGATGGCGGCGGTGCCGGAGGCC
GACGTGGTGATCACCAACCCGACCCACTTCGCCGTAGCCCTGAAGTACGACCCGGCGGGCGGCGGGGCGCCG
CTGCTGCTGGCCAAGGGCAACGATTTCCTGGCCCTGAAGATCCGCGAGGTGGCCCAGGAGCACAAGGTGATG
GTCATGGAGTCGCCGGCGCTGGCGCGAGCGGTGTACTACTCCACCGAGCTGGACCAGGAGATTCCCGCCGGG
CTCTATCTCGCCGTGGCCCAGGTGCTGGCCTATGTCTACCAGCTCAAGCAGTACCAGGCCGGCAAGGGCAAG
CGGCCGAGCCCGTTGAAGGACCTGCCGATCCCGCCGGACCTGCGTCGCGACGAGTGA
Translation:
MAESESGQDKTEEPTEKRRREAREKGQLPRSRELNTLAILMAGAGGLLIYGADLAGALLRLMRSNFELSRET
AMNTESMLQLLGASAYLAAQGLWPILLMLLVAAIVGPIALGGWLFSMDALQPKFSRLNPLSGLKRMFSAKSL
LELSKALIKFLVVLAVALLVLSADRDALLALAHQPLEQAILHSVRVVGWSAFWMACSLLLIAAVDVPYQIWD
NRQKLLMTKQEVRDEYKDSEGKPEVKSKIRQMQREMAQRRMMAAVPEADVVITNPTHFAVALKYDPAGGGAP
LLLAKGNDFLALKIREVAQEHKVMVMESPALARAVYYSTELDQEIPAGLYLAVAQVLAYVYQLKQYQAGKGK
RPSPLKDLPIPPDLRRDE*
AnnotationID:1770GeneID:1761
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 10:10:32
Gene Name:flhB
Confidence Code:2
GeneProduct:flagellar biosynthetic protein FlhB
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1377,pfam01312,COG4792
Secondary Functional Category(ies):intracellular trafficking and secretion
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69930.1|AE016632_1 (AE016790) flagellar biosynthetic protein FlhB [Pseudomonas putida
           KT2440]
          Length = 380

 Score =  520 bits (1339), Expect = e-147
 Identities = 258/379 (68%), Positives = 321/379 (84%), Gaps = 2/379 (0%)

>gb|AAO55493.1| (AE016862) flagellar biosynthetic protein FlhB [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 378

 Score =  496 bits (1277), Expect = e-140
 Identities = 239/377 (63%), Positives = 314/377 (83%)

>gb|AAF95265.1| (AE004285) flagellar biosynthetic protein FlhB [Vibrio cholerae]
          Length = 376

 Score =  357 bits (915), Expect = 9e-98
 Identities = 183/376 (48%), Positives = 271/376 (72%), Gaps = 2/376 (0%)








 
Structural Features:
COG1377, FlhB, Flagellar biosynthesis pathway, component FlhB 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 363 residues, 100.0% aligned
Score =  445 bits (1147), Expect = 3e-126
Identities = 179/363 (49%), Positives = 248/363 (68%)

pfam01312, Bac_export_2, FlhB HrpN YscU SpaS Family. 
CD-Length = 343 residues,  99.7% aligned
Score =  308 bits (791), Expect = 6e-85
Identities = 138/343 (40%), Positives = 211/343 (61%), Gaps = 1/343 (0%)

COG4792, EscU, Type III secretory pathway, component EscU 
[Intracellular trafficking and secretion]
CD-Length = 349 residues,  96.0% aligned
Score =  214 bits (547), Expect = 1e-56
Identities = 109/337 (32%), Positives = 191/337 (56%), Gaps = 2/337 (0%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3525




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20672




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37364




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1761_cdd.html


    		




 
URL:

















    ReferenceID:37970




  
Author/Investigator(s):
        Minamino T, Iino T, Kutuskake K.





  
Title:
        Molecular characterization of the Salmonella typhimurium flhB operon and its protein products.




  
PubMed:
        8002587


MedLine:
        




    
Source:
J Bacteriol. 1994 Dec;176(24):7630-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8002587

















    ReferenceID:37972




  
Author/Investigator(s):
        Hardham JM, Frye JG, Stamm LV.





  
Title:
        Identification and sequences of the Treponema pallidum fliM', fliY, fliP, fliQ, fliR and flhB' genes.




  
PubMed:
        8529894


MedLine:
        




    
Source:
Gene. 1995 Dec 1;166(1):57-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8529894









           
Homologs By Global Alignment 

           

       



     
Gene ID:1761
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3116
                           PA1449_tr
                           translation of PA1449
                           378
                           99.99
                           99.99
                           0
                           1882.0
     

     
     
96202
                           gb|AAG04838.1|AE004574_9
                           (AE004574) flagellar biosynthetic protein FlhB [Pseudomonas aeruginosa PAO1]
                           378
                           99.99
                           99.99
                           0
                           1882.0
     

     
     
96203
                           gb|AAD56298.2|
                           (AF031418) FlhB [Pseudomonas putida]
                           380
                           68.68
                           84.21
                           2
                           1352.0
     

     
     
96204
                           gb|AAN69930.1|AE016632_1
                           (AE016790) flagellar biosynthetic protein FlhB [Pseudomonas putida KT2440]
                           380
                           67.89
                           84.47
                           2
                           1343.0
     

     
     
96205
                           gb|AAO55493.1|
                           (AE016862) flagellar biosynthetic protein FlhB [Pseudomonas syringae pv. tomato str. DC3000]
                           378
                           63.22
                           83.06
                           0
                           1276.0
     

     






      

          
GC ORFID: 44763How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1449Glimmer Score: 

          
Start: 886015Stop: 887151

          
Length: 1137


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1449 translation of PA1449Homolog Bit Score 729.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7934 (ORF: BLASTX 495.0), t_PA1449 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCGAGAGCGAGAGCGGGCAGGACAAGACAGAGGAACCCACGGAGAAACGCCGCCGCGAGGCGCGCGAG 
AAAGGGCAGTTGCCGCGTTCGCGGGAGCTGAACACCCTGGCCATCCTCATGGCCGGCGCCGGCGGCCTGCTG 
ATCTACGGCGCCGACCTGGCGGGCGCCCTGTTGCGCCTGATGCGCAGCAATTTCGAGCTGTCCCGGGAAACC 
GCGATGAATACCGAGAGCATGCTGCAATTGCTCGGCGCTTCGGCCTACCTGGCTGCCCAGGGGCTCTGGCCG 
ATCCTGCTGATGCTGCTGGTGGCGGCGATCGTCGGGCCGATCGCCCTCGGCGGCTGGCTGTTCTCGATGGAC 
GCCCTGCAACCCAAGTTCAGCCGGCTCAACCCGCTGTCGGGGCTGAAGCGGATGTTTTCCGCCAAGTCGCTG 
CTCGAACTGAGCAAGGCGCTGATCAAGTTCCTGGTGGTGCTGGCGGTGGCCCTGCTGGTGCTCTCCGCCGAC 
CGCGACGCCCTCCTGGCCCTGGCGCACCAGCCGCTCGAGCAGGCGATCCTGCACAGCGTGCGGGTGGTCGGC 
TGGAGCGCCTTCTGGATGGCCTGCAGCCTGCTGCTGATCGCCGCGGTGGACGTGCCCTACCAGATCTGGGAC 
AACCGCCAGAAGCTGCTGATGACCAAGCAGGAAGTCCGCGACGAATACAAGGACTCCGAGGGCAAGCCCGAG 
GTCAAGTCGAAGATCCGCCAGATGCAGCGCGAGATGGCGCAGAGACGGATGATGGCGGCGGTGCCGGAGGCC 
GACGTGGTGATCACCAACCCGACCCACTTCGCCGTAGCCCTGAAGTACGACCCGGCGGGCGGCGGGGCGCCG 
CTGCTGCTGGCCAAGGGCAACGATTTCCTGGCCCTGAAGATCCGCGAGGTGGCCCAGGAGCACAAGGTGATG 
GTCATGGAGTCGCCGGCGCTGGCGCGAGCGGTGTACTACTCCACCGAGCTGGACCAGGAGATTCCCGCCGGG 
CTCTATCTCGCCGTGGCCCAGGTGCTGGCCTATGTCTACCAGCTCAAGCAGTACCAGGCCGGCAAGGGCAAG 
CGGCCGAGCCCGTTGAAGGACCTGCCGATCCCGCCGGACCTGCGTCGCGACGAGTGA