ViewGene
Locus Name:
GeneID:
Location:

GeneID:1757
TrivialName: PA14_10230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_10230
PAO1 Ortholog Locus:PA4153
Sequence Length:1092
Protein Length:363
Start: 881136
Stop: 880045
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07540.1|AE004831_12 (AE004831) 2,3-butanediol dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 361/363 (99%), Positives = 362/363 (99%)
Sequence:
ATGAACAGTTCCCCGCAAGCCAACGCGGCGATGCGCGCCGCCGTCTGGCACGGCCGCCACGATATCCGCGTG
GAAGACGTGCCGCTCCCGGCCGAGCCGCCGCCCGGCTGGGTGCAGATCCGCGTGCACTGGTGCGGCATCTGC
GGCTCCGACCTGCACGAATACCTGGCCGGCCCGGTATTCATCCCCGTGGAGGCGCCGCACCCGCTCACCGGC
CTCAAGGATCAATGCATCCTCGGCCACGAGTTCAGCGGCGAAATCGTCCGCCTCGGCAACGGCGTGACCGGC
TTCGCCCTCGGCCAGGCGGTCGCCGCAGACGCCTGCCAGCATTGCGGCACCTGCTACTACTGCCGGCATGGG
CTGTACAACATCTGCGAGAACCTGGCCTTCACCGGGCTGATGAACAACGGCGCCTTCGCCGAATACGTCAAC
GTACCGGCCAACCTGCTCTACGCCCTGCCGGCCGGATTTCCCAGCGAGGCCGGCGCGCTGATCGAGCCGCTG
GCGGTGGGCATGCACGCGGTGAAGAAGGCCGGCAGCCTGCTCGGGCAGAACGTGGTGGTGGTCGGCGCCGGC
ACCATCGGCCTGTGCACCATCATGTGCGCGCGCGCCGCCGGCGCGGCACAGGTGATCGCCCTGGAGATGTCC
TCGGCGCGCAAGGCCAAGGCCCTGGAGGTCGGCGCCAGCCAGGTGCTCGACCCCTCGCGGTGCGACGCCCTA
GGCGAAATCCGCGCCCTCACCGGCGGGCTTGGCGCCGACGTCAGCTTCGAATGCATCGGCAACAAGCACACC
GCCAAGCTGGCCATCGATGCCATTCGCAAGGCCGGCAAATGCGTGCTTGTGGGAATCTTCGAGGAGCCCAGT
GAATTCAATTTCTTCGAGCTGGTTTCCACCGAGAAGCAGTTGCTCGGGGCCCTCGCCTACAACGGCGAGTTC
GCCGACGTGATCGCCTTCATAGCCGACGGCCGCCTCGACATCGCACCGCTGGTGACCGGTCGCATCGGCCTG
GAAGAAATCGTCGAGCGCGGTTTCGAAGAGTTGGTGAACAACAAGGAGCACAACGTGAAGATCATCGTCTCG
CCGGGCGGCTGA
Translation:
MNSSPQANAAMRAAVWHGRHDIRVEDVPLPAEPPPGWVQIRVHWCGICGSDLHEYLAGPVFIPVEAPHPLTG
LKDQCILGHEFSGEIVRLGNGVTGFALGQAVAADACQHCGTCYYCRHGLYNICENLAFTGLMNNGAFAEYVN
VPANLLYALPAGFPSEAGALIEPLAVGMHAVKKAGSLLGQNVVVVGAGTIGLCTIMCARAAGAAQVIALEMS
SARKAKALEVGASQVLDPSRCDALGEIRALTGGLGADVSFECIGNKHTAKLAIDAIRKAGKCVLVGIFEEPS
EFNFFELVSTEKQLLGALAYNGEFADVIAFIADGRLDIAPLVTGRIGLEEIVERGFEELVNNKEHNVKIIVS
PGG*
AnnotationID:1766GeneID:1757
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 16:04:18
Gene Name:adh
Confidence Code:2
GeneProduct:2,3-butanediol dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1063,pfam00107,COG1064
Secondary Functional Category(ies):
EC Number:1.1.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAB58982.1| (L35343) 2,3-butanediol dehydrogenase [Pseudomonas putida]
          Length = 362

 Score =  636 bits (1641), Expect = 0.0
 Identities = 312/352 (88%), Positives = 327/352 (92%)

>gb|AAP07682.1| (AE017000) (R,R)-butanediol dehydrogenase [Bacillus cereus ATCC
           14579]
          Length = 350

 Score =  308 bits (790), Expect = 3e-83
 Identities = 163/352 (46%), Positives = 223/352 (63%), Gaps = 4/352 (1%)








 
Structural Features:
COG1063, Tdh, Threonine dehydrogenase and related Zn-dependent dehydrogenases 
[Amino acid transport and metabolism]
CD-Length = 350 residues,  99.7% aligned
Score =  270 bits (691), Expect = 2e-73
Identities = 142/362 (39%), Positives = 188/362 (51%), Gaps = 24/362 (6%)

pfam00107, ADH_zinc_N, Zinc-binding dehydrogenase.
CD-Length = 341 residues,  99.7% aligned
Score =  236 bits (604), Expect = 2e-63
Identities = 118/352 (33%), Positives = 179/352 (50%), Gaps = 19/352 (5%)

COG1064, AdhP, Zn-dependent alcohol dehydrogenases
CD-Length = 339 residues,  95.9% aligned
Score =  187 bits (476), Expect = 2e-48
Identities = 116/347 (33%), Positives = 169/347 (48%), Gaps = 28/347 (8%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3517




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20664




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36882




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1757_cdd.html


    		




 
URL:

















    ReferenceID:36992




  
Author/Investigator(s):
        Huang M, Oppermann FB, Steinbuchel A.





  
Title:
        Molecular characterization of the Pseudomonas putida 2,3-butanediol catabolic pathway.




  
PubMed:
        7813883


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 Dec 1;124(2):141-50.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=95113288&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:1757
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3111
                           PA4153_tr
                           translation of PA4153
                           363
                           99.44
                           99.72
                           0
                           1887.0
     

     
     
96136
                           gb|AAG07540.1|AE004831_12
                           (AE004831) 2,3-butanediol dehydrogenase [Pseudomonas aeruginosa PAO1]
                           363
                           99.44
                           99.72
                           0
                           1887.0
     

     
     
96137
                           gb|AAN66179.1|AE016245_8
                           (AE016776) 2,3-butanediol dehydrogenase [Pseudomonas putida KT2440]
                           364
                           86.26
                           90.65
                           3
                           1642.0
     

     
     
96138
                           gb|AAB58982.1|
                           (L35343) 2,3-butanediol dehydrogenase [Pseudomonas putida]
                           364
                           86.26
                           90.93
                           3
                           1636.0
     

     
     
96139
                           gb|AAP07682.1|
                           (AE017000) (R,R)-butanediol dehydrogenase [Bacillus cereus ATCC 14579]
                           364
                           44.78
                           61.26
                           15
                           796.0
     

     






      

          
GC ORFID: 44759How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4153Glimmer Score: 

          
Start: 4072801Stop: 4073892

          
Length: 1092


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4153 translation of PA4153Homolog Bit Score 731.0

          
Other Homologs:  t_PA4097 (ORF: BLASTX 271.0), t_PA4153 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACAGTTCCCCGCAAGCCAACGCGGCGATGCGCGCCGCCGTCTGGCACGGCCGCCACGATATCCGCGTG 
GAAGACGTGCCGCTCCCGGCCGAGCCGCCGCCCGGCTGGGTGCAGATCCGCGTGCACTGGTGCGGCATCTGC 
GGCTCCGACCTGCACGAATACCTGGCCGGCCCGGTATTCATCCCCGTGGAGGCGCCGCACCCGCTCACCGGC 
CTCAAGGATCAATGCATCCTCGGCCACGAGTTCAGCGGCGAAATCGTCCGCCTCGGCAACGGCGTGACCGGC 
TTCGCCCTCGGCCAGGCGGTCGCCGCAGACGCCTGCCAGCATTGCGGCACCTGCTACTACTGCCGGCATGGG 
CTGTACAACATCTGCGAGAACCTGGCCTTCACCGGGCTGATGAACAACGGCGCCTTCGCCGAATACGTCAAC 
GTACCGGCCAACCTGCTCTACGCCCTGCCGGCCGGATTTCCCAGCGAGGCCGGCGCGCTGATCGAGCCGCTG 
GCGGTGGGCATGCACGCGGTGAAGAAGGCCGGCAGCCTGCTCGGGCAGAACGTGGTGGTGGTCGGCGCCGGC 
ACCATCGGCCTGTGCACCATCATGTGCGCGCGCGCCGCCGGCGCGGCACAGGTGATCGCCCTGGAGATGTCC 
TCGGCGCGCAAGGCCAAGGCCCTGGAGGTCGGCGCCAGCCAGGTGCTCGACCCCTCGCGGTGCGACGCCCTA 
GGCGAAATCCGCGCCCTCACCGGCGGGCTTGGCGCCGACGTCAGCTTCGAATGCATCGGCAACAAGCACACC 
GCCAAGCTGGCCATCGATGCCATTCGCAAGGCCGGCAAATGCGTGCTTGTGGGAATCTTCGAGGAGCCCAGT 
GAATTCAATTTCTTCGAGCTGGTTTCCACCGAGAAGCAGTTGCTCGGGGCCCTCGCCTACAACGGCGAGTTC 
GCCGACGTGATCGCCTTCATAGCCGACGGCCGCCTCGACATCGCACCGCTGGTGACCGGTCGCATCGGCCTG 
GAAGAAATCGTCGAGCGCGGTTTCGAAGAGTTGGTGAACAACAAGGAGCACAACGTGAAGATCATCGTCTCG 
CCGGGCGGCTGA