ViewGene
Locus Name:
GeneID:
Location:

GeneID:1750
TrivialName: PA14_05530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05530
PAO1 Ortholog Locus:PA0425
Sequence Length:1152
Protein Length:383
Start: 486775
Stop: 487926
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03814.1|AE004479_9 (AE004479) RND multidrug efflux membrane fusion protein MexA precursor [Pseudomonas aeruginosa PAO1]
Identities = 383/383 (100%), Positives = 383/383 (100%)
Sequence:
ATGCAACGAACGCCAGCCATGCGTGTACTGGTTCCGGCCCTGCTGGTCGCGATTTCGGCCCTTTCCGGGTGC
GGAAAAAGCGAGGCGCCGCCGCCGGCGCAAACGCCGGAGGTCGGAATCGTGACCCTGGAAGCGCAGACGGTG
ACCCTGAATACCGAGCTGCCGGGCCGGACCAATGCGTTCCGCATCGCCGAGGTGCGTCCCCAGGTGAACGGC
ATCATCCTCAAGCGCCTGTTCAAGGAAGGCAGCGACGTCAAGGCCGGGCAGCAGCTCTACCAGATCGACCCC
GCCACCTACGAGGCCGACTACCAGAGCGCCCAGGCCAACCTGGCCTCGACCCAGGAGCAGGCCCAGCGCTAC
AAGCTGCTGGTCGCCGACCAGGCCGTGAGCAAGCAGCAGTACGCCGACGCCAATGCCGCCTACCTGCAGTCC
AAGGCGGCGGTGGAACAGGCGCGGATCAACCTGCGCTACACCAAGGTGCTGTCGCCGATCTCCGGCCGCATC
GGCCGTTCCGCGGTGACCGAAGGCGCCCTGGTGACCAACGGCCAGGCCAACGCGATGGCCACCGTGCAACAG
CTCGACCCGATCTACGTCGACGTCACCCAGCCGTCCACCGCCCTGCTGCGCCTGCGCCGCGAACTGGCCAGC
GGCCAGTTGGAGCGCGCCGGCGACAACGCTGCGAAGGTCTCCCTGAAGCTGGAGGACGGTAGCCAATACCCG
CTGGAAGGCCGCCTCGAATTCTCCGAGGTTTCCGTCGACGAAGGCACCGGCTCGGTCACCATCCGCGCCGTG
TTCCCCAACCCGAACAACGAGCTGCTGCCCGGCATGTTCGTTCACGCGCAGTTGCAGGAAGGCGTCAAGCAG
AAGGCCATCCTCGCTCCGCAGCAAGGCGTGACCCGCGACCTCAAGGGCCAGGCTACCGCGCTGGTGGTGAAC
GCGCAGAACAAGGTCGAGCTGCGGGTGATCAAGGCCGACCGGGTGATCGGCGACAAGTGGCTGGTCACCGAA
GGCCTGAACGCCGGCGACAAGATCATTACCGAAGGCCTGCAGTTCGTGCAGCCGGGTGTCGAGGTGAAGACC
GTGCCGGCGAAGAATGTCGCGTCCGCGCAGAAGGCCGACGCCGCTCCGGCGAAAACCGACAGCAAGGGCTGA
Translation:
MQRTPAMRVLVPALLVAISALSGCGKSEAPPPAQTPEVGIVTLEAQTVTLNTELPGRTNAFRIAEVRPQVNG
IILKRLFKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQEQAQRYKLLVADQAVSKQQYADANAAYLQS
KAAVEQARINLRYTKVLSPISGRIGRSAVTEGALVTNGQANAMATVQQLDPIYVDVTQPSTALLRLRRELAS
GQLERAGDNAAKVSLKLEDGSQYPLEGRLEFSEVSVDEGTGSVTIRAVFPNPNNELLPGMFVHAQLQEGVKQ
KAILAPQQGVTRDLKGQATALVVNAQNKVELRVIKADRVIGDKWLVTEGLNAGDKIITEGLQFVQPGVEVKT
VPAKNVASAQKADAAPAKTDSKG*
AnnotationID:1759GeneID:1750
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 09:09:12
Gene Name:mexA
Confidence Code:1
GeneProduct:RND multidrug efflux membrane fusion protein MexA precursor
Cell Localization:()
Synonyms:arpA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:acriflavin resistance protein A precursor
Functional Category Confidence Code:5
COGs:pfam00529,COG0845
Secondary Functional Category(ies):antibiotic resistance and susceptibility
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67009.1|AE016329_4 (AE016779) multidrug/solvent RND membrane fusion protein
           [Pseudomonas putida KT2440]
          Length = 384

 Score =  492 bits (1266), Expect = e-138
 Identities = 255/383 (66%), Positives = 310/383 (80%), Gaps = 2/383 (0%)

>gb|AAO57754.1| (AE016871) efflux transporter, RND family, MFP subunit [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 386

 Score =  479 bits (1234), Expect = e-135
 Identities = 252/384 (65%), Positives = 304/384 (79%), Gaps = 3/384 (0%)

>emb|CAE33021.1| (BX640444) acriflavine resistance protein a precursor [Bordetella
           bronchiseptica]
          Length = 396

 Score =  400 bits (1029), Expect = e-111
 Identities = 222/374 (59%), Positives = 272/374 (72%), Gaps = 16/374 (4%)






 
Structural Features:
pfam00529, HlyD, HlyD family secretion protein.
CD-Length = 309 residues, 100.0% aligned
Score =  154 bits (390), Expect = 1e-38
Identities = 104/310 (33%), Positives = 143/310 (46%), Gaps = 26/310 (8%)

COG0845, AcrA, Membrane-fusion protein 
[Cell envelope biogenesis, outer membrane]
CD-Length = 372 residues,  98.7% aligned
Score =  116 bits (290), Expect = 4e-27
Identities = 126/381 (33%), Positives = 181/381 (47%), Gaps = 32/381 (8%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3503




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20650




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33750




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1750_cdd.html


    		




 
URL:

















    ReferenceID:35363




  
Author/Investigator(s):
        Poole K, Krebes K, McNally C, Neshat S.





  
Title:
        Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon.




  
PubMed:
        8226684


MedLine:
        




    
Source:
J Bacteriol. 1993 Nov;175(22):7363-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8226684









           
Homologs By Global Alignment 

           

       



     
Gene ID:1750
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3071
                           PA0425_tr
                           translation of PA0425
                           383
                           99.99
                           99.99
                           0
                           1890.0
     

     
     
94557
                           gb|AAG03814.1|AE004479_9
                           (AE004479) RND multidrug efflux membrane fusion protein MexA precursor [Pseudomonas aeruginosa PAO1]
                           383
                           99.99
                           99.99
                           0
                           1890.0
     

     
     
94558
                           gb|AAA74436.1|
                           (L11616) envC homologue; ORFA [Pseudomonas aeruginosa]
                           383
                           99.99
                           99.99
                           0
                           1890.0
     

     
     
94559
                           gb|AAQ92180.1|
                           (AY349612) membrane fusion protein [Pseudomonas fluorescens]
                           386
                           66.83
                           78.75
                           4
                           1271.0
     

     
     
94560
                           gb|AAN67009.1|AE016329_4
                           (AE016779) multidrug/solvent RND membrane fusion protein [Pseudomonas putida KT2440]
                           385
                           66.23
                           80.51
                           3
                           1270.0
     

     






      

          
GC ORFID: 44752How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0425Glimmer Score: 

          
Start: 6288117Stop: 6289268

          
Length: 1152


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0425 translation of PA0425Homolog Bit Score 732.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7334 (ORF: BLASTX 341.0), t_PA4599 (ORF: BLASTX 283.0), psyr_15may02_Scaffold1_revised_gene220 (ORF: BLASTX 265.0), t_PA0425 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAACGAACGCCAGCCATGCGTGTACTGGTTCCGGCCCTGCTGGTCGCGATTTCGGCCCTTTCCGGGTGC 
GGAAAAAGCGAGGCGCCGCCGCCGGCGCAAACGCCGGAGGTCGGAATCGTGACCCTGGAAGCGCAGACGGTG 
ACCCTGAATACCGAGCTGCCGGGCCGGACCAATGCGTTCCGCATCGCCGAGGTGCGTCCCCAGGTGAACGGC 
ATCATCCTCAAGCGCCTGTTCAAGGAAGGCAGCGACGTCAAGGCCGGGCAGCAGCTCTACCAGATCGACCCC 
GCCACCTACGAGGCCGACTACCAGAGCGCCCAGGCCAACCTGGCCTCGACCCAGGAGCAGGCCCAGCGCTAC 
AAGCTGCTGGTCGCCGACCAGGCCGTGAGCAAGCAGCAGTACGCCGACGCCAATGCCGCCTACCTGCAGTCC 
AAGGCGGCGGTGGAACAGGCGCGGATCAACCTGCGCTACACCAAGGTGCTGTCGCCGATCTCCGGCCGCATC 
GGCCGTTCCGCGGTGACCGAAGGCGCCCTGGTGACCAACGGCCAGGCCAACGCGATGGCCACCGTGCAACAG 
CTCGACCCGATCTACGTCGACGTCACCCAGCCGTCCACCGCCCTGCTGCGCCTGCGCCGCGAACTGGCCAGC 
GGCCAGTTGGAGCGCGCCGGCGACAACGCTGCGAAGGTCTCCCTGAAGCTGGAGGACGGTAGCCAATACCCG 
CTGGAAGGCCGCCTCGAATTCTCCGAGGTTTCCGTCGACGAAGGCACCGGCTCGGTCACCATCCGCGCCGTG 
TTCCCCAACCCGAACAACGAGCTGCTGCCCGGCATGTTCGTTCACGCGCAGTTGCAGGAAGGCGTCAAGCAG 
AAGGCCATCCTCGCTCCGCAGCAAGGCGTGACCCGCGACCTCAAGGGCCAGGCTACCGCGCTGGTGGTGAAC 
GCGCAGAACAAGGTCGAGCTGCGGGTGATCAAGGCCGACCGGGTGATCGGCGACAAGTGGCTGGTCACCGAA 
GGCCTGAACGCCGGCGACAAGATCATTACCGAAGGCCTGCAGTTCGTGCAGCCGGGTGTCGAGGTGAAGACC 
GTGCCGGCGAAGAATGTCGCGTCCGCGCAGAAGGCCGACGCCGCTCCGGCGAAAACCGACAGCAAGGGCTGA