ViewGene
Locus Name:
GeneID:
Location:

GeneID:1742
TrivialName: PA14_50520
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_50520
PAO1 Ortholog Locus:PA1074
Sequence Length:1122
Protein Length:373
Start: 4486950
Stop: 4488071
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04463.1|AE004539_5 (AE004539) branched-chain amino acid transport protein BraC [Pseudomonas aeruginosa PAO1]
Identities = 372/373 (99%), Positives = 372/373 (99%)
Sequence:
ATGAAGAAGGGTACTCAGCGTCTATCCCGCCTGTTCGCCGCGATGGCCATTGCCGGGTTCGCCAGCTACTCC
ATGGCCGCCGACACCATCAAGATCGCCCTGGCTGGCCCGGTCACCGGTCCGGTAGCCCAGTACGGCGACATG
CAGCGCGCCGGTGCGCTGATGGCAATCGAACAGATCAACAAGGCAGGTGGCGTGAACGGCGCGCAACTCGAA
GGCGTGATCTACGACGACGCCTGCGATCCCAAGCAGGCCGTGGCGGTCGCCAACAAGGTGGTCAACGACGGC
GTCAAGTTCGTGGTCGGCCATGTCTGCTCCAGCTCCACCCAACCCGCCACCGACATCTACGAAGACGAAGGC
GTGCTGATGATCACCCCGTCAGCCACCGCCCCGGAAATCACCTCGCGCGGCTACAAACTGATCTTCCGCACC
ATCGGCCTGGACAACATGCAGGGCCCGGTGGCCGGCAAGTTCATCGCCGAACGCTACAAGGACAAGACCATC
GCGGTACTGCACGACAAGCAGCAGTACGGCGAAGGCATCGCCACCGAGGTGAAGAAGACAGTGGAAGACGCC
GGCATCAAGGTTGCCGTCTTCGAAGGCCTGAACGCCGGCGACAAGGACTTCAACGCGCTGATCAGCAAGCTG
AAGAAAGCCGGCGTGCAGTTCGTCTACTTCGGCGGCTACCACCCGGAAATGGGCCTGCTGCTGCGCCAGGCC
AAGCAGGCCGGGCTGGACGCGCGCTTCATGGGCCCGGAAGGGGTCGGCAACAGCGAAATCACCGCGATCGCC
GGCGACGCCTCGGAAGGCATGCTGGCGACCCTGCCGCGCGCCTTCGAGCAGGATCCGAAGAACAAGGCCCTG
ATCGACGCCTTCAAGGCGAAGAACCAGGATCCGAGCGGCATCTTCGTCCTGCCCGCCTACTCCGCGGTCACC
GTGATCGCCAAGGGCATCGAGAAAGCCGGCGAGGCCGATCCGGAGAAGGTCGCCGCGGCCCTGCGCGCCAAC
ACCTTCGAGACTCCCACCGGGAACCTCGGGTTCGACGAGAAGGGCGACCTGAAGAACTTCGACTTCACCGTC
TACGAGTGGCACAAGGACGCCACCCGTACCGAGGTCAAGTAA
Translation:
MKKGTQRLSRLFAAMAIAGFASYSMAADTIKIALAGPVTGPVAQYGDMQRAGALMAIEQINKAGGVNGAQLE
GVIYDDACDPKQAVAVANKVVNDGVKFVVGHVCSSSTQPATDIYEDEGVLMITPSATAPEITSRGYKLIFRT
IGLDNMQGPVAGKFIAERYKDKTIAVLHDKQQYGEGIATEVKKTVEDAGIKVAVFEGLNAGDKDFNALISKL
KKAGVQFVYFGGYHPEMGLLLRQAKQAGLDARFMGPEGVGNSEITAIAGDASEGMLATLPRAFEQDPKNKAL
IDAFKAKNQDPSGIFVLPAYSAVTVIAKGIEKAGEADPEKVAAALRANTFETPTGNLGFDEKGDLKNFDFTV
YEWHKDATRTEVK*
AnnotationID:1751GeneID:1742
AnnotatorUID: danlee
Modification Date/Time: 2004-05-19 17:05:31
Gene Name:braC
Confidence Code:1
GeneProduct:branched-chain amino acid transport protein BraC
Cell Localization:(8) Periplasmic
Synonyms:PA1074
Cell Localization Confidence Code:1
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0683
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
COG0683, LivK, ABC-type branched-chain amino acid transport systems, periplasmic component
       CD-Length = 366 residues,  97.3% aligned
       Score =  268 bits (686), Expect = 7e-73
pfam01094, ANF_receptor, Receptor family ligand binding region. Sc






   
Genomic Context:
Adjacent to genes BraD, BraE, BraF, and BraG, reported to constitute a
transport system for branched-chain amino acids 






   
Comment:


















    ReferenceID:3487




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20634




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32222




  
Author/Investigator(s):
        Hoshino T, Kose K.





  
Title:
        Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.




  
PubMed:
        91008919


MedLine:
        




    
Source:
J Bacteriol. 1990 Oct;172(10):5531-9






    
Reference Type:Journal 







    
Data:


    		




 
URL:

















    ReferenceID:32223




  
Author/Investigator(s):
        Hoshino T, Kose K.





  
Title:
        Cloning and nucleotide sequence of braC, the structural gene for the leucine-, isoleucine-, and valine-binding protein of Pseudomonas aeruginosa PAO.





  
PubMed:
        90036723


MedLine:
        




    
Source:
J Bacteriol. 1989 Nov;171(11):6300-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1742
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3057
                           PA1074_tr
                           translation of PA1074
                           373
                           99.73
                           99.73
                           0
                           1892.0
     

     
     
94336
                           gb|AAG04463.1|AE004539_5
                           (AE004539) branched-chain amino acid transport protein BraC [Pseudomonas aeruginosa PAO1]
                           373
                           99.73
                           99.73
                           0
                           1892.0
     

     
     
94337
                           dbj|BAA14254.1|
                           (D90223) periplasmic protein [Pseudomonas aeruginosa]
                           373
                           99.73
                           99.73
                           0
                           1892.0
     

     
     
94338
                           gb|AAA88430.1|
                           (M31071) leucine-, isoleucine-, and valine-binding protein [Pseudomonas aeruginosa]
                           373
                           99.46
                           99.46
                           0
                           1883.0
     

     
     
94339
                           gb|AAR19309.1|
                           (AY426537) putative branched-chain amino acid-binding protein [symbiont bacterium of Paederus fuscipes]
                           373
                           86.86
                           92.22
                           1
                           1681.0
     

     






      

          
GC ORFID: 44744How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1074Glimmer Score: 

          
Start: 466958Stop: 465837

          
Length: 1122


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA1074 translation of PA1074Homolog Bit Score 733.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6073 (ORF: BLASTX 534.0), t_PA4913 (ORF: BLASTX 290.0), psyr_15may02_Scaffold8_revised_gene7641 (ORF: BLASTX 276.0), t_PA1074 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGAAGGGTACTCAGCGTCTATCCCGCCTGTTCGCCGCGATGGCCATTGCCGGGTTCGCCAGCTACTCC 
ATGGCCGCCGACACCATCAAGATCGCCCTGGCTGGCCCGGTCACCGGTCCGGTAGCCCAGTACGGCGACATG 
CAGCGCGCCGGTGCGCTGATGGCAATCGAACAGATCAACAAGGCAGGTGGCGTGAACGGCGCGCAACTCGAA 
GGCGTGATCTACGACGACGCCTGCGATCCCAAGCAGGCCGTGGCGGTCGCCAACAAGGTGGTCAACGACGGC 
GTCAAGTTCGTGGTCGGCCATGTCTGCTCCAGCTCCACCCAACCCGCCACCGACATCTACGAAGACGAAGGC 
GTGCTGATGATCACCCCGTCAGCCACCGCCCCGGAAATCACCTCGCGCGGCTACAAACTGATCTTCCGCACC 
ATCGGCCTGGACAACATGCAGGGCCCGGTGGCCGGCAAGTTCATCGCCGAACGCTACAAGGACAAGACCATC 
GCGGTACTGCACGACAAGCAGCAGTACGGCGAAGGCATCGCCACCGAGGTGAAGAAGACAGTGGAAGACGCC 
GGCATCAAGGTTGCCGTCTTCGAAGGCCTGAACGCCGGCGACAAGGACTTCAACGCGCTGATCAGCAAGCTG 
AAGAAAGCCGGCGTGCAGTTCGTCTACTTCGGCGGCTACCACCCGGAAATGGGCCTGCTGCTGCGCCAGGCC 
AAGCAGGCCGGGCTGGACGCGCGCTTCATGGGCCCGGAAGGGGTCGGCAACAGCGAAATCACCGCGATCGCC 
GGCGACGCCTCGGAAGGCATGCTGGCGACCCTGCCGCGCGCCTTCGAGCAGGATCCGAAGAACAAGGCCCTG 
ATCGACGCCTTCAAGGCGAAGAACCAGGATCCGAGCGGCATCTTCGTCCTGCCCGCCTACTCCGCGGTCACC 
GTGATCGCCAAGGGCATCGAGAAAGCCGGCGAGGCCGATCCGGAGAAGGTCGCCGCGGCCCTGCGCGCCAAC 
ACCTTCGAGACTCCCACCGGGAACCTCGGGTTCGACGAGAAGGGCGACCTGAAGAACTTCGACTTCACCGTC 
TACGAGTGGCACAAGGACGCCACCCGTACCGAGGTCAAGTAA