ViewGene
Locus Name:
GeneID:
Location:

GeneID:1731
TrivialName: PA14_03930
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03930
PAO1 Ortholog Locus:PA0301
Sequence Length:1098
Protein Length:365
Start: 354084
Stop: 355181
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03690.1|AE004468_2 (AE004468) polyamine transport protein [Pseudomonas aeruginosa PAO1]
Identities = 365/365 (100%), Positives = 365/365 (100%)
Sequence:
ATGCAACATTCCATTGGCAAGACACTGCTGGTAGCCGCTCTGGCTACGGCGATCGCGGGCCCCGTGCAGGCG
GAGAAGAAGAGCCTGCACATCTATAACTGGACCGATTACATCGCTCCCACCACCCTCAAGGACTTCACCAAG
GAGAGCGGGATCGACGTCAGCTACGACGTCTTCGACTCCAACGAGACCCTCGAGGGCAAGCTGGTCTCCGGC
CACTCCGGCTATGACATCGTGGTGCCGTCGAACAACTTCCTCGGCAAGCAGATCCAGGCCGGTGCCTTCCAG
AAGCTGGACAAGAGCAAGCTGCCGAACTGGAAGAACCTCGATCCGGCGCTGCTCAAGCAACTGGAGGTCAGC
GACCCGGGCAACCAGTACGCGGTTCCCTACCTGTGGGGCACCAACGGCATCGGTTACAACGTGGCCAAGGTC
AAGGAAGTCCTCGGCGACCAGCCGATCGACTCCTGGGCCATCCTCTTCGAGCCGGAGAACATGAAGAAGCTC
GCCAAGTGCGGCGTAGCCTTCATGGACAGCGGCGACGAGATGCTGCCCGCCGCCCTCAACTACCTGGGGCTG
GACCCCAACACCCACGACCCGAAGGACTACAAGAAGGCCGAGGAAGTCCTGACCAAGGTTCGCCCCTACGTC
AGCTACTTCCACTCCTCCAAGTACATCTCCGACCTGGCCAACGGCAACATCTGCGTCGCCTTCGGCTATTCC
GGGGACGTCTTCCAGGCCGCTGCCCGCGCCGAAGAGGCCGGCAAGGGCATCGACATCCAGTACGTGATTCCC
AAGGAAGGCGCCAACCTGTGGTTCGACCTGATGGCGATTCCCGCCGATGCCAAGGCCGCCGATAACGCCTAT
GCGTTCATCGACTACCTGCTGCGTCCGGAGGTGATCGCCAAGGTCAGCGACTACGTCGGCTACGCCAACGCG
ATACCGGGGGCCCGCCCGCTGATGGACAAGAGCGTCAGCGACAGCGAGGAGGTGTATCCGCCGCAGGCCGTG
CTCGACAAGCTCTACGTTTCCGCCGTGTTGCCGGCCAAGGTGCTGCGCCTGCAGACCCGTACCTGGACCCGG
ATCAAGACCGGCAAATAA
Translation:
MQHSIGKTLLVAALATAIAGPVQAEKKSLHIYNWTDYIAPTTLKDFTKESGIDVSYDVFDSNETLEGKLVSG
HSGYDIVVPSNNFLGKQIQAGAFQKLDKSKLPNWKNLDPALLKQLEVSDPGNQYAVPYLWGTNGIGYNVAKV
KEVLGDQPIDSWAILFEPENMKKLAKCGVAFMDSGDEMLPAALNYLGLDPNTHDPKDYKKAEEVLTKVRPYV
SYFHSSKYISDLANGNICVAFGYSGDVFQAAARAEEAGKGIDIQYVIPKEGANLWFDLMAIPADAKAADNAY
AFIDYLLRPEVIAKVSDYVGYANAIPGARPLMDKSVSDSEEVYPPQAVLDKLYVSAVLPAKVLRLQTRTWTR
IKTGK*
AnnotationID:1740GeneID:1731
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 07:07:58
Gene Name:spuE
Confidence Code:1
GeneProduct: polyamine transport protein
Cell Localization:()
Synonyms:potF2
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:polyamine ABC transporter, periplasmic polyamine-binding protein
Functional Category Confidence Code:5
COGs:COG0687
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70745.1|AE016718_4 (AE016793) putrescine ABC transporter, periplasmic
           putrescine-binding protein [Pseudomonas putida KT2440]
          Length = 364

 Score =  514 bits (1324), Expect = e-145
 Identities = 249/365 (68%), Positives = 299/365 (81%), Gaps = 1/365 (0%)

>gb|AAO58732.1| (AE016875) putrescine ABC transporter, periplasmic
           putrescine-binding protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 374

 Score =  484 bits (1245), Expect = e-136
 Identities = 238/361 (65%), Positives = 284/361 (78%), Gaps = 1/361 (0%)

>gb|AAN54338.1|AE015571_1 (AE015571) polyamine ABC transporter, periplasmic polyamine-binding
           protein [Shewanella oneidensis MR-1]
          Length = 367

 Score =  449 bits (1156), Expect = e-126
 Identities = 216/360 (60%), Positives = 283/360 (78%), Gaps = 3/360 (0%)








 
Structural Features:
COG0687, PotD, Spermidine/putrescine-binding periplasmic protein 
[Amino acid transport and metabolism]
CD-Length = 363 residues,  97.8% aligned
Score =  351 bits (901), Expect = 8e-98
Identities = 175/363 (48%), Positives = 219/363 (60%), Gaps = 9/363 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3465




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20612




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33650




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1731_cdd.html


    		




 
URL:

















    ReferenceID:35430




  
Author/Investigator(s):
        Lu CD, Itoh Y, Nakada Y, Jiang Y.





  
Title:
        Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1.




  
PubMed:
        12081945


MedLine:
        




    
Source:
J Bacteriol. 2002 Jul;184(14):3765-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12081945









           
Homologs By Global Alignment 

           

       



     
Gene ID:1731
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3038
                           PA0301_tr
                           translation of PA0301
                           365
                           99.99
                           99.99
                           0
                           1902.0
     

     
     
93974
                           gb|AAG03690.1|AE004468_2
                           (AE004468) polyamine transport protein [Pseudomonas aeruginosa PAO1]
                           365
                           99.99
                           99.99
                           0
                           1902.0
     

     
     
93976
                           gb|AAN70745.1|AE016718_4
                           (AE016793) putrescine ABC transporter, periplasmic putrescine-binding protein [Pseudomonas putida KT2440]
                           365
                           68.21
                           81.91
                           1
                           1326.0
     

     
     
93975
                           gb|AAK15488.1|AF323694_3
                           (AF323694) PotF2 [Pseudomonas fluorescens]
                           371
                           67.11
                           82.47
                           12
                           1330.0
     

     
     
93978
                           gb|AAN68755.1|AE016507_6
                           (AE016785) periplasmic polyamine-binding protein, putative [Pseudomonas putida KT2440]
                           365
                           63.83
                           78.08
                           3
                           1242.0
     

     






      

          
GC ORFID: 44733How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0301Glimmer Score: 

          
Start: 6155426Stop: 6156523

          
Length: 1098


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0301 translation of PA0301Homolog Bit Score 737.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6512 (ORF: BLASTX 498.0), t_PA0300 (ORF: BLASTX 455.0), t_PA1410 (ORF: BLASTX 443.0), t_PA2711 (ORF: BLASTX 438.0), psyr_15may02_Scaffold6_revised_gene6511 (ORF: BLASTX 426.0), psyr_15may02_Scaffold7_revised_gene7441 (ORF: BLASTX 379.0), t_PA2592 (ORF: BLASTX 364.0), t_PA0301 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAACATTCCATTGGCAAGACACTGCTGGTAGCCGCTCTGGCTACGGCGATCGCGGGCCCCGTGCAGGCG 
GAGAAGAAGAGCCTGCACATCTATAACTGGACCGATTACATCGCTCCCACCACCCTCAAGGACTTCACCAAG 
GAGAGCGGGATCGACGTCAGCTACGACGTCTTCGACTCCAACGAGACCCTCGAGGGCAAGCTGGTCTCCGGC 
CACTCCGGCTATGACATCGTGGTGCCGTCGAACAACTTCCTCGGCAAGCAGATCCAGGCCGGTGCCTTCCAG 
AAGCTGGACAAGAGCAAGCTGCCGAACTGGAAGAACCTCGATCCGGCGCTGCTCAAGCAACTGGAGGTCAGC 
GACCCGGGCAACCAGTACGCGGTTCCCTACCTGTGGGGCACCAACGGCATCGGTTACAACGTGGCCAAGGTC 
AAGGAAGTCCTCGGCGACCAGCCGATCGACTCCTGGGCCATCCTCTTCGAGCCGGAGAACATGAAGAAGCTC 
GCCAAGTGCGGCGTAGCCTTCATGGACAGCGGCGACGAGATGCTGCCCGCCGCCCTCAACTACCTGGGGCTG 
GACCCCAACACCCACGACCCGAAGGACTACAAGAAGGCCGAGGAAGTCCTGACCAAGGTTCGCCCCTACGTC 
AGCTACTTCCACTCCTCCAAGTACATCTCCGACCTGGCCAACGGCAACATCTGCGTCGCCTTCGGCTATTCC 
GGGGACGTCTTCCAGGCCGCTGCCCGCGCCGAAGAGGCCGGCAAGGGCATCGACATCCAGTACGTGATTCCC 
AAGGAAGGCGCCAACCTGTGGTTCGACCTGATGGCGATTCCCGCCGATGCCAAGGCCGCCGATAACGCCTAT 
GCGTTCATCGACTACCTGCTGCGTCCGGAGGTGATCGCCAAGGTCAGCGACTACGTCGGCTACGCCAACGCG 
ATACCGGGGGCCCGCCCGCTGATGGACAAGAGCGTCAGCGACAGCGAGGAGGTGTATCCGCCGCAGGCCGTG 
CTCGACAAGCTCTACGTTTCCGCCGTGTTGCCGGCCAAGGTGCTGCGCCTGCAGACCCGTACCTGGACCCGG 
ATCAAGACCGGCAAATAA