ViewGene
Locus Name:
GeneID:
Location:

GeneID:1730
TrivialName: PA14_47300
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47300
PAO1 Ortholog Locus:PA1310
Sequence Length:1116
Protein Length:371
Start: 4214307
Stop: 4213192
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04699.1|AE004560_7 (AE004560) 2-aminoethylphosphonate:pyruvate aminotransferase [Pseudomonas aeruginosa PAO1]
Identities = 370/371 (99%), Positives = 370/371 (99%)
Sequence:
ATGAGCACTGCCGAACGCGCACCTATCCTGCTGACCCCCGGTCCGCTGACCACCTCCTACCGTACCCGTCGG
GCGATGATGGTCGACTGGGGCTCCTGGGACAGCGATTTCAACGAACTCACCGCCAGCGTCTGCCAGCGCCTG
CTGAAGATCGTCGGCGGCGAAGGCAGCCACACCTGCGTACCGTTGCAGGGCAGCGGCACCTTCGCCGTGGAA
GCGGCGATCGGCACCCTGGTGCCGCGCGACGGCAAGGTCCTGGTGTTGATCAACGGTGCCTACGGCAAGCGC
CTGGCGAAGATCTGCGAGGTCCTGCAACGGCCCTTCAGCACCTTCGAGACCGAAGAGAACGTGCCGACCACC
GCCGCTGACGTCGAGCGCCTGCTGGCCGCCGATCCGGCGATCAGCCATGTGGCGCTGATCCACTGCGAGACC
AGCACCGGCATCCTCAATCCGCTCGAGGCCATCGCCAAGGTCGTCGAGCGTCACGGCAAGCGCCTGATCGTC
GATGCCATGAGCTCCTTCGGGGCGATCGGGATCGATGCCCGGAAAGTTCCGTTCGATGCCTTGATCGCCGCC
TCCGGCAAGTGCCTGGAGGGCGTCCCCGGGATGGGCTTCGTCTTCGCCCGCAGCGCCGCCCTGGAAGCCAGC
GCCGGCAACTGCCACTCCCTGGCCATGGACCTGCAGGACCAGCACGCCTATATGCGCAAGACCGGGCAGTGG
CGCTTCACCCCGCCGACCCATGTGGTCGCCGCCCTGCACGAAGCGCTCAGCCAGTACGAAGAGGAGGGCGGC
CTGCCGGCGCGCCAGCGGCGCTACGCGAGCAATTGCGAAACGCTGCTCGGCGAGATGGCCCGGCTCGGTTTC
CGCAGCTTCCTGCCGGCGGAAATCCAGGCGCCGATCATCGTTACCTTCCACGCGCCGCGCGATCCGCGCTAC
CGCTTCGCCGACTTCTACCAACGCGTGCGGGAGAAGGGCTTCATCCTCTATCCGGGCAAGTTGACCCAGGTG
GAAACCTTCCGCGTCGGCTGCATCGGCCACGTCGACGCCGCGGAGATGCGGCAGGCGGTCGCCGCCATCGGC
GAGGCGCTGCGCGAACTGGAAGTCCTCGAGATCTGA
Translation:
MSTAERAPILLTPGPLTTSYRTRRAMMVDWGSWDSDFNELTASVCQRLLKIVGGEGSHTCVPLQGSGTFAVE
AAIGTLVPRDGKVLVLINGAYGKRLAKICEVLQRPFSTFETEENVPTTAADVERLLAADPAISHVALIHCET
STGILNPLEAIAKVVERHGKRLIVDAMSSFGAIGIDARKVPFDALIAASGKCLEGVPGMGFVFARSAALEAS
AGNCHSLAMDLQDQHAYMRKTGQWRFTPPTHVVAALHEALSQYEEEGGLPARQRRYASNCETLLGEMARLGF
RSFLPAEIQAPIIVTFHAPRDPRYRFADFYQRVREKGFILYPGKLTQVETFRVGCIGHVDAAEMRQAVAAIG
EALRELEVLEI*
AnnotationID:1739GeneID:1730
AnnotatorUID: diggins
Modification Date/Time: 2005-05-26 09:09:40
Gene Name:phnW
Confidence Code:1
GeneProduct:2-aminoethylphosphonate:pyruvate aminotransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0075
Secondary Functional Category(ies):amino acid transport and metabolism
EC Number:2.6.1.37
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67822.1|AE016413_4 (AE016782) 2-aminoethylphosphonate:pyruvate aminotransferase
           [Pseudomonas putida KT2440]
          Length = 368

 Score =  592 bits (1525), Expect = e-168
 Identities = 291/365 (79%), Positives = 323/365 (88%)

>emb|CAC49377.1| (AL603645) putative 2-aminoethylphosphonate: pyruvate
           aminotransferase, similar to rtxA AAA03070 protein
           [Sinorhizobium meliloti]
          Length = 383

 Score =  384 bits (985), Expect = e-106
 Identities = 182/362 (50%), Positives = 261/362 (72%), Gaps = 1/362 (0%)

>dbj|BAC61578.1| (AP005084) 2-aminoethylphosphonate:pyruvate aminotransferase
           [Vibrio parahaemolyticus]
          Length = 367

 Score =  322 bits (824), Expect = 3e-87
 Identities = 158/357 (44%), Positives = 234/357 (65%), Gaps = 2/357 (0%)






 
Structural Features:
COG0075, COG0075, Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase 
[Amino acid transport and metabolism]
CD-Length = 383 residues,  95.0% aligned
Score =  351 bits (902), Expect = 8e-98
Identities = 134/367 (36%), Positives = 181/367 (49%), Gaps = 6/367 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3463




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20610




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37488




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1730_cdd.html


    		




 
URL:

















    ReferenceID:38019




  
Author/Investigator(s):
        Kim AD, Baker AS, Dunaway-Mariano D, Metcalf WW, Wanner BL, Martin BM.





  
Title:
        The 2-aminoethylphosphonate-specific transaminase of the 2-aminoethylphosphonate degradation pathway.




  
PubMed:
        12107130


MedLine:
        




    
Source:
J Bacteriol. 2002 Aug;184(15):4134-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12107130

















    ReferenceID:38173




  
Author/Investigator(s):
        Dumora C, Lacoste AM, Cassaigne A.





  
Title:
        Purification and properties of 2-aminoethylphosphonate:pyruvate aminotransferase from Pseudomonas aeruginosa.




  
PubMed:
        6406228


MedLine:
        




    
Source:
Eur J Biochem. 1983 Jun 1;133(1):119-25.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=83209625&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:1730
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3037
                           PA1310_tr
                           translation of PA1310
                           371
                           99.73
                           99.73
                           0
                           1902.0
     

     
     
93957
                           gb|AAG04699.1|AE004560_7
                           (AE004560) 2-aminoethylphosphonate:pyruvate aminotransferase [Pseudomonas aeruginosa PAO1]
                           371
                           99.73
                           99.73
                           0
                           1902.0
     

     
     
93959
                           emb|CAD24488.1|
                           (AJ437001) putative 2AEP:pyruvate aminotransferase [Pseudomonas putida]
                           371
                           79.24
                           87.06
                           2
                           1510.0
     

     
     
93958
                           gb|AAN67822.1|AE016413_4
                           (AE016782) 2-aminoethylphosphonate:pyruvate aminotransferase [Pseudomonas putida KT2440]
                           371
                           78.43
                           87.06
                           3
                           1524.0
     

     
     
93960
                           emb|CAC49377.1|
                           (AL603645) putative 2-aminoethylphosphonate: pyruvate aminotransferase, similar to rtxA AAA03070 protein [Sinorhizobium meliloti]
                           387
                           47.02
                           67.44
                           20
                           976.0
     

     






      

          
GC ORFID: 44732How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1310Glimmer Score: 

          
Start: 739602Stop: 740717

          
Length: 1116


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1310 translation of PA1310Homolog Bit Score 737.0

          
Other Homologs:  t_PA1310 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCACTGCCGAACGCGCACCTATCCTGCTGACCCCCGGTCCGCTGACCACCTCCTACCGTACCCGTCGG 
GCGATGATGGTCGACTGGGGCTCCTGGGACAGCGATTTCAACGAACTCACCGCCAGCGTCTGCCAGCGCCTG 
CTGAAGATCGTCGGCGGCGAAGGCAGCCACACCTGCGTACCGTTGCAGGGCAGCGGCACCTTCGCCGTGGAA 
GCGGCGATCGGCACCCTGGTGCCGCGCGACGGCAAGGTCCTGGTGTTGATCAACGGTGCCTACGGCAAGCGC 
CTGGCGAAGATCTGCGAGGTCCTGCAACGGCCCTTCAGCACCTTCGAGACCGAAGAGAACGTGCCGACCACC 
GCCGCTGACGTCGAGCGCCTGCTGGCCGCCGATCCGGCGATCAGCCATGTGGCGCTGATCCACTGCGAGACC 
AGCACCGGCATCCTCAATCCGCTCGAGGCCATCGCCAAGGTCGTCGAGCGTCACGGCAAGCGCCTGATCGTC 
GATGCCATGAGCTCCTTCGGGGCGATCGGGATCGATGCCCGGAAAGTTCCGTTCGATGCCTTGATCGCCGCC 
TCCGGCAAGTGCCTGGAGGGCGTCCCCGGGATGGGCTTCGTCTTCGCCCGCAGCGCCGCCCTGGAAGCCAGC 
GCCGGCAACTGCCACTCCCTGGCCATGGACCTGCAGGACCAGCACGCCTATATGCGCAAGACCGGGCAGTGG 
CGCTTCACCCCGCCGACCCATGTGGTCGCCGCCCTGCACGAAGCGCTCAGCCAGTACGAAGAGGAGGGCGGC 
CTGCCGGCGCGCCAGCGGCGCTACGCGAGCAATTGCGAAACGCTGCTCGGCGAGATGGCCCGGCTCGGTTTC 
CGCAGCTTCCTGCCGGCGGAAATCCAGGCGCCGATCATCGTTACCTTCCACGCGCCGCGCGATCCGCGCTAC 
CGCTTCGCCGACTTCTACCAACGCGTGCGGGAGAAGGGCTTCATCCTCTATCCGGGCAAGTTGACCCAGGTG 
GAAACCTTCCGCGTCGGCTGCATCGGCCACGTCGACGCCGCGGAGATGCGGCAGGCGGTCGCCGCCATCGGC 
GAGGCGCTGCGCGAACTGGAAGTCCTCGAGATCTGA