ViewGene
Locus Name:
GeneID:
Location:

GeneID:173
TrivialName: PA14_62760
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62760
PAO1 Ortholog Locus:PA4744
Sequence Length:2523
Protein Length:840
Start: 5601878
Stop: 5599356
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08130.1|AE004888_5 (AE004888) translation initiation factor IF-2 [Pseudomonas aeruginosa PAO1]
Identities = 838/840 (99%), Positives = 838/840 (99%)
Sequence:
ATGACGCAAGTCACGGTGAAAGAACTGGCCCAGGTGGTCGACACACCGGTGGAGCGCCTGCTGCTGCAGATG
CGTGACGCAGGTCTGCCGCACACCAGTGCCGAACAAGTTGTAACGGATAGCGAGAAGCAAGCCCTGCTGACC
CACCTGAAAGGCAGCCATGGCGATCGCGCCAGCGAGCCGCGCAAGATCACCCTGCAACGCAAGACCACTACC
ACGCTGAAAGTGGGTGGCAGCAAGACGGTGAGCGTCGAAGTTCGCAAGAAGAAAACCTATGTCAAGCGCAGC
CCCGACGAGATCGAGGCCGAGCGCCAGCGCGAACTCGAGGAGCAGCGCGCGGCGGAAGAGGCGGAGCGTCTG
AAGGCCGAAGAGGCCGCTGCCCGCCAGCGTGCCGAAGAGGAAGCGCGCAAGGCCGAGGAAGCTGCGCGTGCC
AAGGCTGCCCAGGAAGCAGCGGCTACTGCCGGTGCCGAGCCTGCCGTGGTGGCGGATGTCGCGGCTGCCGAA
CCGGTGGCCAAGCCTGCCGCCGTGGAAGAACGCAAGAAGGAAGAGCCGCGCCGCGCGCCCAAGCGTGACGAG
GACGATGACCGCCGCGACCGCAAGCACACCCAGCACCGTCCCTCGGTCAAGGAGAAGGAAAAGGTTCCTGCT
CCGCGCGTGGCTCCACGCAGCACCGACGAGGAAAGCGATGGTTACCGTCGCGGCGGTCGTGGTGGCAAGTCG
AAGCTGAAGAAGCGCAACCAGCACGGGTTCCAGAACCCGACAGGACCGATCGTGCGTGAAGTGAACATCGGT
GAGACCATCACCGTAGCCGAGTTGGCCGCCCAGATGTCGGTCAAGGGTGCCGAAGTCGTCAAGTTCATGTTC
AAGATGGGCTCCCCGGTGACCATCAACCAGGTCCTGGACCAGGAGACCGCCCAACTGGTCGCCGAAGAGCTG
GGCCACAAGGTCAAGCTGGTCAGCGAGAACGCGCTGGAAGAACAACTGGCCGAGTCCCTGAAGTTCGAAGGC
GAGGCGGTCACCCGCGCGCCGGTCGTCACCGTCATGGGCCACGTCGACCACGGCAAGACCTCGCTGCTCGAC
TACATCCGTCGCGCCAAGGTCGCCGCGGGCGAGGCCGGTGGCATCACCCAGCATATCGGTGCCTACCACGTC
GAAACCGAGCGTGGCATGGTGACCTTCCTGGATACCCCCGGCCACGCCGCGTTCACCGCGATGCGTGCCCGT
GGCGCCCAGGCGACCGACATCGTGATCCTGGTGGTCGCGGCCGACGACGGCGTCATGCCGCAGACCCAGGAA
GCCGTGCAGCACGCGAAAGCGGCGGGCGTGCCGATCGTGGTCGCGGTGAACAAGATCGACAAGCCCGAGGCC
AACCCGGACAACATCAAGAACGGCCTGGCCGCTCTCGACGTGATCCCGGAAGAGTGGGGCGGTGACGCTCCC
TTCGTCCCGGTTTCGGCGAAGCTCGGTACTGGCGTCGATGAACTGCTCGAAGCGGTCCTGCTGCAGGCAGAA
GTGCTCGAACTGAAAGCCACTCCGTCGGCCCCGGGCCGTGGCGTGGTGGTCGAGTCGCGCCTGGACAAGGGA
CGCGGCCCGGTGGCCACCGTGCTGGTCCAGGACGGTACCCTGCGCCAGGGCGACATGGTGCTGGTCGGCATC
AACTACGGTCGTGTCCGCGCCATGCTCGACGAGAACGGCAAGCCGATCAAGGAAGCCGGCCCGTCGATCCCG
GTCGAGATCCTCGGCCTGGACGGTACGCCGGATGCCGGTGACGAGATGACCGTGGTCGCCGACGAGAAGAAG
GCCCGCGAAGTCGCCCTGTTCCGTCAAGGCAAGTTCCGTGAGGTGAAACTGGCGCGTGCCCATGCCGGCAAG
CTGGAAAACATCTTCGAGAACATGGGCCAGGAAGAGAAGAAGACCCTGAACATCGTCCTCAAGGCCGATGTG
CGCGGTTCGCTCGAGGCTCTGCAAGGCTCGCTCAGCGGCCTGGGCAACGACGAAGTGCAGGTCCGCGTGGTC
GGTGGCGGCGTCGGTGGCATCACCGAGTCCGACGCCAACCTGGCGCTGGCTTCCAACGCGGTGCTGTTCGGC
TTCAACGTGCGCGCCGACGCCGGCGCGCGCAAGATCGTCGAAGCCGAAGGTCTGGATATGCGTTACTACAAC
GTGATCTACGACATCATCGAGGACGTCAAGAAGGCCCTGACCGGCATGCTCGGCAGCGATCTGCGGGAGAAC
ATCCTGGGCATCGCCGAGGTGCGCGACGTGTTCCGCTCGCCGAAGTTCGGCGCGATCGCCGGCTGCATGGTC
ACCGAGGGCATGGTGCACCGTAACCGTCCGATCCGCGTGCTGCGGGACGATGTGGTCATCTTCGAAGGCGAG
CTGGAGTCTCTCCGTCGCTTCAAGGACGACGTCGCCGAAGTGCGTGCCGGCATGGAGTGCGGCATCGGCGTG
AAGAGCTACAACGACGTCAAGGTCGGCGACAAGATCGAAGTGTTCGAGAAGGTCGAAGTCGCACGCAGCCTT
TGA
Translation:
MTQVTVKELAQVVDTPVERLLLQMRDAGLPHTSAEQVVTDSEKQALLTHLKGSHGDRASEPRKITLQRKTTT
TLKVGGSKTVSVEVRKKKTYVKRSPDEIEAERQRELEEQRAAEEAERLKAEEAAARQRAEEEARKAEEAARA
KAAQEAAATAGAEPAVVADVAAAEPVAKPAAVEERKKEEPRRAPKRDEDDDRRDRKHTQHRPSVKEKEKVPA
PRVAPRSTDEESDGYRRGGRGGKSKLKKRNQHGFQNPTGPIVREVNIGETITVAELAAQMSVKGAEVVKFMF
KMGSPVTINQVLDQETAQLVAEELGHKVKLVSENALEEQLAESLKFEGEAVTRAPVVTVMGHVDHGKTSLLD
YIRRAKVAAGEAGGITQHIGAYHVETERGMVTFLDTPGHAAFTAMRARGAQATDIVILVVAADDGVMPQTQE
AVQHAKAAGVPIVVAVNKIDKPEANPDNIKNGLAALDVIPEEWGGDAPFVPVSAKLGTGVDELLEAVLLQAE
VLELKATPSAPGRGVVVESRLDKGRGPVATVLVQDGTLRQGDMVLVGINYGRVRAMLDENGKPIKEAGPSIP
VEILGLDGTPDAGDEMTVVADEKKAREVALFRQGKFREVKLARAHAGKLENIFENMGQEEKKTLNIVLKADV
RGSLEALQGSLSGLGNDEVQVRVVGGGVGGITESDANLALASNAVLFGFNVRADAGARKIVEAEGLDMRYYN
VIYDIIEDVKKALTGMLGSDLRENILGIAEVRDVFRSPKFGAIAGCMVTEGMVHRNRPIRVLRDDVVIFEGE
LESLRRFKDDVAEVRAGMECGIGVKSYNDVKVGDKIEVFEKVEVARSL*
AnnotationID:183GeneID:173
AnnotatorUID: diggins
Modification Date/Time: 2005-03-09 08:08:09
Gene Name:infB
Confidence Code:2
GeneProduct:translation initiation factor IF-2
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0532,pfam00009
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57938.1| (AE016872) translation initiation factor IF-2 [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 841

 Score = 1323 bits (3423), Expect = 0.0
 Identities = 693/846 (81%), Positives = 754/846 (89%), Gaps = 11/846 (1%)

>gb|AAN70284.1|AE016669_2 (AE016791) translation initiation factor IF-2 [Pseudomonas putida
           KT2440]
          Length = 846

 Score = 1318 bits (3411), Expect = 0.0
 Identities = 690/848 (81%), Positives = 751/848 (88%), Gaps = 10/848 (1%)

>gb|AAO10112.1|AE016802_155 (AE016802) Translation initiation factor 2 [Vibrio vulnificus
           CMCP6]
          Length = 907

 Score =  964 bits (2492), Expect = 0.0
 Identities = 538/910 (59%), Positives = 650/910 (71%), Gaps = 74/910 (8%)






 
Structural Features:
COG0532, InfB, Translation initiation factor 2 (IF-2; GTPase) 
[Translation, ribosomal structure and biogenesis]
CD-Length = 509 residues,  99.4% aligned
Score =  673 bits (1737), Expect = 0.0
Identities = 326/506 (64%), Positives = 391/506 (77%), Gaps = 4/506 (0%)

pfam00009, GTP_EFTU, Elongation factor Tu GTP binding domain. 
CD-Length = 211 residues,  92.9% aligned
Score =  144 bits (365), Expect = 3e-35
Identities = 67/196 (34%), Positives = 94/196 (47%), Gaps = 29/196 (14%)






   
Genomic Context:






   
Comment:


















    ReferenceID:349




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17491




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36557




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:173_cdd.html


    		




 
URL:

















    ReferenceID:36608




  
Author/Investigator(s):
        Hedegaard J, Hauge M, Fage-Larsen J, Mortensen KK, Kilian M, Sperling-Petersen HU, Poulsen K.





  
Title:
        Investigation of the translation-initiation factor IF2 gene, infB, as a tool to study the population structure of Streptococcus agalactiae.




  
PubMed:
        10878130


MedLine:
        




    
Source:
Microbiology. 2000 Jul;146 ( Pt 7):1661-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10878130

















    ReferenceID:36609




  
Author/Investigator(s):
        Sacerdot C, Dessen P, Hershey JW, Plumbridge JA, Grunberg-Manago M.





  
Title:
        Sequence of the initiation factor IF2 gene: unusual protein features and homologies with elongation factors.




  
PubMed:
        6096856


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1984 Dec;81(24):7787-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=6096856

















    ReferenceID:36610




  
Author/Investigator(s):
        Friedrich K, Brombach M, Pon CL.





  
Title:
        Identification, cloning and sequence of the Streptococcus faecium infB (translational initiation factor IF2) gene.




  
PubMed:
        3063954


MedLine:
        




    
Source:
Mol Gen Genet. 1988 Nov;214(3):595-600.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3063954

















    ReferenceID:36611




  
Author/Investigator(s):
        Shazand K, Tucker J, Chiang R, Stansmore K, Sperling-Petersen HU, Grunberg-Manago M, Rabinowitz JC, Leighton T.





  
Title:
        Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2.




  
PubMed:
        2110148


MedLine:
        




    
Source:
J Bacteriol. 1990 May;172(5):2675-87.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2110148









           
Homologs By Global Alignment 

           

       



     
Gene ID:173
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
308
                           PA4744_tr
                           translation of PA4744
                           840
                           99.76
                           99.76
                           0
                           4176.0
     

     
     
19424
                           gb|AAG08130.1|AE004888_5
                           (AE004888) translation initiation factor IF-2 [Pseudomonas aeruginosa PAO1]
                           840
                           99.76
                           99.76
                           0
                           4176.0
     

     
     
19425
                           gb|AAO57938.1|
                           (AE016872) translation initiation factor IF-2 [Pseudomonas syringae pv. tomato str. DC3000]
                           847
                           82.05
                           89.37
                           13
                           3437.0
     

     
     
19426
                           gb|AAN70284.1|AE016669_2
                           (AE016791) translation initiation factor IF-2 [Pseudomonas putida KT2440]
                           850
                           81.52
                           88.58
                           14
                           3427.0
     

     
     
19427
                           gb|AAO10112.1|AE016802_155
                           (AE016802) Translation initiation factor 2 [Vibrio vulnificus CMCP6]
                           913
                           59.47
                           71.41
                           79
                           2548.0
     

     






      

          
GC ORFID: 43175How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4744Glimmer Score: 

          
Start: 4865558Stop: 4863036

          
Length: 2523


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4744 translation of PA4744Homolog Bit Score 1613.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5493 (ORF: BLASTX 1318.0), t_PA4744 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACGCAAGTCACGGTGAAAGAACTGGCCCAGGTGGTCGACACACCGGTGGAGCGCCTGCTGCTGCAGATG 
CGTGACGCAGGTCTGCCGCACACCAGTGCCGAACAAGTTGTAACGGATAGCGAGAAGCAAGCCCTGCTGACC 
CACCTGAAAGGCAGCCATGGCGATCGCGCCAGCGAGCCGCGCAAGATCACCCTGCAACGCAAGACCACTACC 
ACGCTGAAAGTGGGTGGCAGCAAGACGGTGAGCGTCGAAGTTCGCAAGAAGAAAACCTATGTCAAGCGCAGC 
CCCGACGAGATCGAGGCCGAGCGCCAGCGCGAACTCGAGGAGCAGCGCGCGGCGGAAGAGGCGGAGCGTCTG 
AAGGCCGAAGAGGCCGCTGCCCGCCAGCGTGCCGAAGAGGAAGCGCGCAAGGCCGAGGAAGCTGCGCGTGCC 
AAGGCTGCCCAGGAAGCAGCGGCTACTGCCGGTGCCGAGCCTGCCGTGGTGGCGGATGTCGCGGCTGCCGAA 
CCGGTGGCCAAGCCTGCCGCCGTGGAAGAACGCAAGAAGGAAGAGCCGCGCCGCGCGCCCAAGCGTGACGAG 
GACGATGACCGCCGCGACCGCAAGCACACCCAGCACCGTCCCTCGGTCAAGGAGAAGGAAAAGGTTCCTGCT 
CCGCGCGTGGCTCCACGCAGCACCGACGAGGAAAGCGATGGTTACCGTCGCGGCGGTCGTGGTGGCAAGTCG 
AAGCTGAAGAAGCGCAACCAGCACGGGTTCCAGAACCCGACAGGACCGATCGTGCGTGAAGTGAACATCGGT 
GAGACCATCACCGTAGCCGAGTTGGCCGCCCAGATGTCGGTCAAGGGTGCCGAAGTCGTCAAGTTCATGTTC 
AAGATGGGCTCCCCGGTGACCATCAACCAGGTCCTGGACCAGGAGACCGCCCAACTGGTCGCCGAAGAGCTG 
GGCCACAAGGTCAAGCTGGTCAGCGAGAACGCGCTGGAAGAACAACTGGCCGAGTCCCTGAAGTTCGAAGGC 
GAGGCGGTCACCCGCGCGCCGGTCGTCACCGTCATGGGCCACGTCGACCACGGCAAGACCTCGCTGCTCGAC 
TACATCCGTCGCGCCAAGGTCGCCGCGGGCGAGGCCGGTGGCATCACCCAGCATATCGGTGCCTACCACGTC 
GAAACCGAGCGTGGCATGGTGACCTTCCTGGATACCCCCGGCCACGCCGCGTTCACCGCGATGCGTGCCCGT 
GGCGCCCAGGCGACCGACATCGTGATCCTGGTGGTCGCGGCCGACGACGGCGTCATGCCGCAGACCCAGGAA 
GCCGTGCAGCACGCGAAAGCGGCGGGCGTGCCGATCGTGGTCGCGGTGAACAAGATCGACAAGCCCGAGGCC 
AACCCGGACAACATCAAGAACGGCCTGGCCGCTCTCGACGTGATCCCGGAAGAGTGGGGCGGTGACGCTCCC 
TTCGTCCCGGTTTCGGCGAAGCTCGGTACTGGCGTCGATGAACTGCTCGAAGCGGTCCTGCTGCAGGCAGAA 
GTGCTCGAACTGAAAGCCACTCCGTCGGCCCCGGGCCGTGGCGTGGTGGTCGAGTCGCGCCTGGACAAGGGA 
CGCGGCCCGGTGGCCACCGTGCTGGTCCAGGACGGTACCCTGCGCCAGGGCGACATGGTGCTGGTCGGCATC 
AACTACGGTCGTGTCCGCGCCATGCTCGACGAGAACGGCAAGCCGATCAAGGAAGCCGGCCCGTCGATCCCG 
GTCGAGATCCTCGGCCTGGACGGTACGCCGGATGCCGGTGACGAGATGACCGTGGTCGCCGACGAGAAGAAG 
GCCCGCGAAGTCGCCCTGTTCCGTCAAGGCAAGTTCCGTGAGGTGAAACTGGCGCGTGCCCATGCCGGCAAG 
CTGGAAAACATCTTCGAGAACATGGGCCAGGAAGAGAAGAAGACCCTGAACATCGTCCTCAAGGCCGATGTG 
CGCGGTTCGCTCGAGGCTCTGCAAGGCTCGCTCAGCGGCCTGGGCAACGACGAAGTGCAGGTCCGCGTGGTC 
GGTGGCGGCGTCGGTGGCATCACCGAGTCCGACGCCAACCTGGCGCTGGCTTCCAACGCGGTGCTGTTCGGC 
TTCAACGTGCGCGCCGACGCCGGCGCGCGCAAGATCGTCGAAGCCGAAGGTCTGGATATGCGTTACTACAAC 
GTGATCTACGACATCATCGAGGACGTCAAGAAGGCCCTGACCGGCATGCTCGGCAGCGATCTGCGGGAGAAC 
ATCCTGGGCATCGCCGAGGTGCGCGACGTGTTCCGCTCGCCGAAGTTCGGCGCGATCGCCGGCTGCATGGTC 
ACCGAGGGCATGGTGCACCGTAACCGTCCGATCCGCGTGCTGCGGGACGATGTGGTCATCTTCGAAGGCGAG 
CTGGAGTCTCTCCGTCGCTTCAAGGACGACGTCGCCGAAGTGCGTGCCGGCATGGAGTGCGGCATCGGCGTG 
AAGAGCTACAACGACGTCAAGGTCGGCGACAAGATCGAAGTGTTCGAGAAGGTCGAAGTCGCACGCAGCCTT 
TGA