ViewGene
Locus Name:
GeneID:
Location:

GeneID:1728
TrivialName: PA14_56410
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56410
PAO1 Ortholog Locus:PA4339
Sequence Length:1080
Protein Length:359
Start: 5032396
Stop: 5031317
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07727.1|AE004850_5 (AE004850) probable phospholipase [Pseudomonas aeruginosa PAO1]
Identities = 358/359 (99%), Positives = 358/359 (99%)
Sequence:
ATGCTGCTGGCCATCGAGGCGGCGCAGCGGAGCATCGAGCTGGAGCTGTACCTGGTGGAAGACGGGCATTGC
GCCGAACTGTTTCTCGTCGCCCTGCTGGATGCCCGCCGCCGGGGCGTCGCCGTGCGCTGCCTGTTCGACGGC
TTCGGCTGCCTCGGCCTGGGCAGCGCCTGGATCCAGCGCCTGCGCGAGGCCGGCGGCGAACTGCGGCTGTAC
AACCCGCTGCGCTGGAAGCTGACTGGCGGCAATCTCTACCGCGACCATCGCAAGCTGCTGCTGGTGGACGGG
CGCCTCGGCTATGTCGGCGGCGCCGGGATCACCGACGAATTCTGGGAACCGGTCAGCGACGTCAGCGCCTGG
CGCGAGGTGATGGTCGAGATGGACGGCCCGGTGGTGGCGGACTGGGCCGCGCTGTTCGAGCGGCAATGGCTG
GCCTGTCTCGAAGAGAAAGCCTGGAAGCCCCGTGAGAGCATGACCCTTACCCGCCTTCCGCCGCAGCCGGGG
GCGGCCCGTGGGTTGGGCCGGGTGGCCTACGCCGACGCCCGCCAGCACCGCGACATCCTGCAATCGCTGGTC
CGCGCGCTGAACGGCTCGCGCCGACGGATCTGGCTGGCGACGCCGTACTTCCTGCCGACCTGGAAGGTTCGC
CGGGCCCTGCGCAAGGCCGCCCAGCGCGGCGTGGAGGTGCGCCTGCTGCTGGCCGGGCGGCTCACCGACCAC
GCCCCGGTGCGCTATGCCGGGCAGCGCTACTACCCGCGCCTGCTGCGCGCCGGCGTGCGCATCCACGAATAC
CAGCCGCGCTTCCTGCACCTGAAGATGGTCATGGTCGACGATTGGGTGAGCGTCGGCTCCTGCAACTTCGAT
CATTGGAATCTACGCTTCAACCTCGACGCCAATCTCGAGGCCCTCGACCCGGACTTCACCAATGAGGCGGCG
GCGAGCCTGCTGGCGGACTTCGCCGACAGCCGCGAGGTGACCCTGGCGATGTGGCGCGCGCGACCCCTGTGG
ATGCGCCTGCACCAGCGCCTGTGGGGTTGGCTGGACCGGGTAGTGGTGAATTTCCTCGACCGCCGGCGCTAG
Translation:
MLLAIEAAQRSIELELYLVEDGHCAELFLVALLDARRRGVAVRCLFDGFGCLGLGSAWIQRLREAGGELRLY
NPLRWKLTGGNLYRDHRKLLLVDGRLGYVGGAGITDEFWEPVSDVSAWREVMVEMDGPVVADWAALFERQWL
ACLEEKAWKPRESMTLTRLPPQPGAARGLGRVAYADARQHRDILQSLVRALNGSRRRIWLATPYFLPTWKVR
RALRKAAQRGVEVRLLLAGRLTDHAPVRYAGQRYYPRLLRAGVRIHEYQPRFLHLKMVMVDDWVSVGSCNFD
HWNLRFNLDANLEALDPDFTNEAAASLLADFADSREVTLAMWRARPLWMRLHQRLWGWLDRVVVNFLDRRR*
AnnotationID:1737GeneID:1728
AnnotatorUID: diggins
Modification Date/Time: 2005-04-04 17:05:05
Gene Name:
Confidence Code:3
GeneProduct:putative phospholipase
Cell Localization:()
Synonyms:PA4339
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1502,cd00138
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66517.1|AE016280_9 (AE016777) phospholipase family protein [Pseudomonas putida KT2440]
          Length = 385

 Score =  484 bits (1245), Expect = e-136
 Identities = 238/358 (66%), Positives = 267/358 (74%)

>gb|AAO57799.1| (AE016871) phospholipase D family protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 385

 Score =  476 bits (1224), Expect = e-133
 Identities = 227/358 (63%), Positives = 272/358 (75%)








 
Structural Features:
COG1502, Cls, Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases 
and related enzymes [Lipid metabolism]
CD-Length = 438 residues,  81.3% aligned
Score =  235 bits (599), Expect = 9e-63
Identities = 125/356 (35%), Positives = 193/356 (54%), Gaps = 13/356 (3%)

cd00138, PLDc, Phospholipase D. Active site motifs
CD-Length = 176 residues,  97.2% aligned
Score = 72.9 bits (178), Expect = 6e-14
Identities = 46/174 (26%), Positives = 70/174 (40%), Gaps = 14/174 (8%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3459




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20606




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36692




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1728_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1728
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3035
                           PA4339_tr
                           translation of PA4339
                           359
                           99.72
                           99.72
                           0
                           1904.0
     

     
     
93926
                           gb|AAG07727.1|AE004850_5
                           (AE004850) probable phospholipase [Pseudomonas aeruginosa PAO1]
                           359
                           99.72
                           99.72
                           0
                           1904.0
     

     
     
93927
                           gb|AAN66517.1|AE016280_9
                           (AE016777) phospholipase family protein [Pseudomonas putida KT2440]
                           385
                           61.81
                           69.35
                           26
                           1243.0
     

     
     
93928
                           gb|AAO57799.1|
                           (AE016871) phospholipase D family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           385
                           58.96
                           70.64
                           26
                           1224.0
     

     






      

          
GC ORFID: 44730How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4339Glimmer Score: 

          
Start: 4296072Stop: 4294993

          
Length: 1080


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4339 translation of PA4339Homolog Bit Score 738.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2077 (ORF: BLASTX 519.0), t_PA4339 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTGCTGGCCATCGAGGCGGCGCAGCGGAGCATCGAGCTGGAGCTGTACCTGGTGGAAGACGGGCATTGC 
GCCGAACTGTTTCTCGTCGCCCTGCTGGATGCCCGCCGCCGGGGCGTCGCCGTGCGCTGCCTGTTCGACGGC 
TTCGGCTGCCTCGGCCTGGGCAGCGCCTGGATCCAGCGCCTGCGCGAGGCCGGCGGCGAACTGCGGCTGTAC 
AACCCGCTGCGCTGGAAGCTGACTGGCGGCAATCTCTACCGCGACCATCGCAAGCTGCTGCTGGTGGACGGG 
CGCCTCGGCTATGTCGGCGGCGCCGGGATCACCGACGAATTCTGGGAACCGGTCAGCGACGTCAGCGCCTGG 
CGCGAGGTGATGGTCGAGATGGACGGCCCGGTGGTGGCGGACTGGGCCGCGCTGTTCGAGCGGCAATGGCTG 
GCCTGTCTCGAAGAGAAAGCCTGGAAGCCCCGTGAGAGCATGACCCTTACCCGCCTTCCGCCGCAGCCGGGG 
GCGGCCCGTGGGTTGGGCCGGGTGGCCTACGCCGACGCCCGCCAGCACCGCGACATCCTGCAATCGCTGGTC 
CGCGCGCTGAACGGCTCGCGCCGACGGATCTGGCTGGCGACGCCGTACTTCCTGCCGACCTGGAAGGTTCGC 
CGGGCCCTGCGCAAGGCCGCCCAGCGCGGCGTGGAGGTGCGCCTGCTGCTGGCCGGGCGGCTCACCGACCAC 
GCCCCGGTGCGCTATGCCGGGCAGCGCTACTACCCGCGCCTGCTGCGCGCCGGCGTGCGCATCCACGAATAC 
CAGCCGCGCTTCCTGCACCTGAAGATGGTCATGGTCGACGATTGGGTGAGCGTCGGCTCCTGCAACTTCGAT 
CATTGGAATCTACGCTTCAACCTCGACGCCAATCTCGAGGCCCTCGACCCGGACTTCACCAATGAGGCGGCG 
GCGAGCCTGCTGGCGGACTTCGCCGACAGCCGCGAGGTGACCCTGGCGATGTGGCGCGCGCGACCCCTGTGG 
ATGCGCCTGCACCAGCGCCTGTGGGGTTGGCTGGACCGGGTAGTGGTGAATTTCCTCGACCGCCGGCGCTAG