ViewGene
Locus Name:
GeneID:
Location:

GeneID:1713
TrivialName: PA14_07190
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_07190
PAO1 Ortholog Locus:PA0552
Sequence Length:1164
Protein Length:387
Start: 621256
Stop: 622419
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03941.1|AE004491_8 (AE004491) phosphoglycerate kinase [Pseudomonas aeruginosa PAO1]
Identities = 387/387 (100%), Positives = 387/387 (100%)
Sequence:
ATGACCGTGTTGAAGATGACTGACCTCGACCTCAAAGGTAAGCGCGTGCTGATCCGCGAGGACCTCAACGTG
CCCGTGAAGGACGGCCAGGTGCAGAGCGATGCACGGATCAAGGCCGCCCTGCCGACCCTCAAGCTGGCCCTG
GAGAAGGGCGCGGCGGTGATGGTCTGCTCGCACCTGGGCCGTCCGACCGAGGGCGAGTTCTCCGCCGAGAAC
AGCCTGAAGCCGGTGGCCGAGTACCTGTCCAAGGCCCTGGGCCGCGAAGTGCCGCTGCTGGCCGACTACCTC
GACGGCGTCGAGGTCAAGGCGGGCGACCTGGTGCTGTTCGAGAACGTGCGCTTCAACAAGGGCGAGAAGAAG
AACGCTGACGAGCTGGCGCAGAAGTACGCCGCGCTGTGCGACGTGTTCGTGATGGATGCCTTCGGTACCGCC
CACCGCGCCGAGGGGTCGACCCACGGCGTGGCCAGGTTCGCCAAGGTCGCCGCCGCCGGCCCGCTGCTGGCC
GCCGAACTGGACGCGCTGGGCAAGGCGCTGGGCAACCCGGCGCGGCCGATGGCGGCGATCGTCGCCGGCTCC
AAGGTTTCCACCAAGCTCGACGTGCTGAACAGCCTGGCCGGCATCTGCGACCAGCTGATCGTCGGCGGCGGC
ATCGCCAACACCTTCCTCGCCGCGGCCGGACACAAGGTCGGCAAGTCGCTGTACGAGGCCGACCTGGTCGAG
ACCGCCAAGGCCATCGCCGCCAAGGTCAAGGTGCCGCTGCCGGTGGACGTGGTGGTGGCCAAGGAATTCGCC
GAGAGCGCCGTGGCGACCGTCAAGGCCATCGCCGAGGTCGCCGACGACGACATGATCCTCGACATCGGCCCG
CAGACCGCCGCGCAGTTCGCCGAGCTGCTGAAGACCTCGAAGACCATCCTGTGGAACGGTCCGGTCGGGGTG
TTCGAGTTCGACCAGTTCGGCGAGGGCACCCGTACCCTGGCCAATGCCATCGCCGATAGCGCGGCGTTCTCC
ATCGCCGGCGGCGGCGATACCCTGGCGGCGATCGACAAGTACGGCATCGCCGAGCGGATCTCCTATATTTCC
ACCGGTGGCGGCGCGTTCCTCGAGTTCGTCGAGGGCAAGGTCCTGCCGGCCGTGGAAATCCTGGAGCAGCGC
GCCAAGGGCTGA
Translation:
MTVLKMTDLDLKGKRVLIREDLNVPVKDGQVQSDARIKAALPTLKLALEKGAAVMVCSHLGRPTEGEFSAEN
SLKPVAEYLSKALGREVPLLADYLDGVEVKAGDLVLFENVRFNKGEKKNADELAQKYAALCDVFVMDAFGTA
HRAEGSTHGVARFAKVAAAGPLLAAELDALGKALGNPARPMAAIVAGSKVSTKLDVLNSLAGICDQLIVGGG
IANTFLAAAGHKVGKSLYEADLVETAKAIAAKVKVPLPVDVVVAKEFAESAVATVKAIAEVADDDMILDIGP
QTAAQFAELLKTSKTILWNGPVGVFEFDQFGEGTRTLANAIADSAAFSIAGGGDTLAAIDKYGIAERISYIS
TGGGAFLEFVEGKVLPAVEILEQRAKG*
AnnotationID:1722GeneID:1713
AnnotatorUID: diggins
Modification Date/Time: 2005-03-29 07:07:38
Gene Name:pgk
Confidence Code:2
GeneProduct:phosphoglycerate kinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00318
Secondary Functional Category(ies):central intermediary metabolism
EC Number:2.7.2.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53931.1| (AE016857) phosphoglycerate kinase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 387

 Score =  678 bits (1749), Expect = 0.0
 Identities = 346/386 (89%), Positives = 372/386 (96%)

>gb|AAN70530.1|AE016695_4 (AE016792) phosphoglycerate kinase [Pseudomonas putida KT2440]
          Length = 387

 Score =  674 bits (1739), Expect = 0.0
 Identities = 349/386 (90%), Positives = 368/386 (95%)

>gb|AAC22183.1| (U32734) phosphoglycerate kinase (pgk) [Haemophilus influenzae Rd]
          Length = 386

 Score =  545 bits (1404), Expect = e-154
 Identities = 280/386 (72%), Positives = 324/386 (83%), Gaps = 1/386 (0%)








 
Structural Features:
cd00318, Phosphoglycerate kinase (PGK) 
CD-Length = 397 residues, 100.0% aligned
Score =  437 bits (1125), Expect = 1e-123
Identities = 203/400 (50%), Positives = 264/400 (66%), Gaps = 25/400 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3429




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20576




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33861




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1713_cdd.html


    		




 
URL:

















    ReferenceID:35287




  
Author/Investigator(s):
        Schlapfer BS, Zuber H.





  
Title:
        Cloning and sequencing of the genes encoding glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase (gap operon) from mesophilic Bacillus megaterium: comparison with corresponding sequences from thermophilic Bacill




  
PubMed:
        1452037


MedLine:
        




    
Source:
Gene. 1992 Dec 1;122(1):53-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1452037

















    ReferenceID:35288




  
Author/Investigator(s):
        Eikmanns BJ.





  
Title:
        Identification, sequence analysis, and expression of a Corynebacterium glutamicum gene cluster encoding the three glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomerase.




  
PubMed:
        1400158


MedLine:
        




    
Source:
J Bacteriol. 1992 Oct;174(19):6076-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1400158

















    ReferenceID:35289




  
Author/Investigator(s):
        Goldman GH, Geremia RA, Caplan AB, Vila SB, Villarroel R, Van Montagu M, Herrera-Estrella A.





  
Title:
        Molecular characterization and regulation of the phosphoglycerate kinase gene from Trichoderma viride.




  
PubMed:
        1588821


MedLine:
        




    
Source:
Mol Microbiol. 1992 May;6(9):1231-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1588821

















    ReferenceID:35290




  
Author/Investigator(s):
        Alefounder PR, Perham RN.





  
Title:
        Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli.




  
PubMed:
        2546007


MedLine:
        




    
Source:
Mol Microbiol. 1989 Jun;3(6):723-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2546007

















    ReferenceID:35291




  
Author/Investigator(s):
        Conway T, Ingram LO.





  
Title:
        Phosphoglycerate kinase gene from Zymomonas mobilis: cloning, sequencing, and localization within the gap operon.




  
PubMed:
        2832389


MedLine:
        




    
Source:
J Bacteriol. 1988 Apr;170(4):1926-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2832389









           
Homologs By Global Alignment 

           

       



     
Gene ID:1713
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3011
                           PA0552_tr
                           translation of PA0552
                           387
                           99.99
                           99.99
                           0
                           1912.0
     

     
     
93521
                           gb|AAG03941.1|AE004491_8
                           (AE004491) phosphoglycerate kinase [Pseudomonas aeruginosa PAO1]
                           387
                           99.99
                           99.99
                           0
                           1912.0
     

     
     
93523
                           gb|AAN70530.1|AE016695_4
                           (AE016792) phosphoglycerate kinase [Pseudomonas putida KT2440]
                           387
                           90.18
                           95.09
                           0
                           1739.0
     

     
     
93522
                           gb|AAO53931.1|
                           (AE016857) phosphoglycerate kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           387
                           89.40
                           96.12
                           0
                           1749.0
     

     
     
93524
                           emb|CAB61570.1|
                           (AJ251129) phosphoglycerate kinase [Pseudomonas sp. 'TAC II 18']
                           387
                           88.11
                           94.57
                           0
                           1711.0
     

     






      

          
GC ORFID: 44715How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0552Glimmer Score: 

          
Start: 6422598Stop: 6423761

          
Length: 1164


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0552 translation of PA0552Homolog Bit Score 741.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6573 (ORF: BLASTX 676.0), t_PA0552 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCGTGTTGAAGATGACTGACCTCGACCTCAAAGGTAAGCGCGTGCTGATCCGCGAGGACCTCAACGTG 
CCCGTGAAGGACGGCCAGGTGCAGAGCGATGCACGGATCAAGGCCGCCCTGCCGACCCTCAAGCTGGCCCTG 
GAGAAGGGCGCGGCGGTGATGGTCTGCTCGCACCTGGGCCGTCCGACCGAGGGCGAGTTCTCCGCCGAGAAC 
AGCCTGAAGCCGGTGGCCGAGTACCTGTCCAAGGCCCTGGGCCGCGAAGTGCCGCTGCTGGCCGACTACCTC 
GACGGCGTCGAGGTCAAGGCGGGCGACCTGGTGCTGTTCGAGAACGTGCGCTTCAACAAGGGCGAGAAGAAG 
AACGCTGACGAGCTGGCGCAGAAGTACGCCGCGCTGTGCGACGTGTTCGTGATGGATGCCTTCGGTACCGCC 
CACCGCGCCGAGGGGTCGACCCACGGCGTGGCCAGGTTCGCCAAGGTCGCCGCCGCCGGCCCGCTGCTGGCC 
GCCGAACTGGACGCGCTGGGCAAGGCGCTGGGCAACCCGGCGCGGCCGATGGCGGCGATCGTCGCCGGCTCC 
AAGGTTTCCACCAAGCTCGACGTGCTGAACAGCCTGGCCGGCATCTGCGACCAGCTGATCGTCGGCGGCGGC 
ATCGCCAACACCTTCCTCGCCGCGGCCGGACACAAGGTCGGCAAGTCGCTGTACGAGGCCGACCTGGTCGAG 
ACCGCCAAGGCCATCGCCGCCAAGGTCAAGGTGCCGCTGCCGGTGGACGTGGTGGTGGCCAAGGAATTCGCC 
GAGAGCGCCGTGGCGACCGTCAAGGCCATCGCCGAGGTCGCCGACGACGACATGATCCTCGACATCGGCCCG 
CAGACCGCCGCGCAGTTCGCCGAGCTGCTGAAGACCTCGAAGACCATCCTGTGGAACGGTCCGGTCGGGGTG 
TTCGAGTTCGACCAGTTCGGCGAGGGCACCCGTACCCTGGCCAATGCCATCGCCGATAGCGCGGCGTTCTCC 
ATCGCCGGCGGCGGCGATACCCTGGCGGCGATCGACAAGTACGGCATCGCCGAGCGGATCTCCTATATTTCC 
ACCGGTGGCGGCGCGTTCCTCGAGTTCGTCGAGGGCAAGGTCCTGCCGGCCGTGGAAATCCTGGAGCAGCGC 
GCCAAGGGCTGA