ViewGene
Locus Name:
GeneID:
Location:

GeneID:1707
TrivialName: PA14_18470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18470
PAO1 Ortholog Locus:PA3547
Sequence Length:1104
Protein Length:367
Start: 1585119
Stop: 1584016
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06935.1|AE004775_6 (AE004775) poly(beta-d-mannuronate) lyase precursor AlgL [Pseudomonas aeruginosa PAO1]
Identities = 367/367 (100%), Positives = 367/367 (100%)
Sequence:
ATGAAAACGTCCCACCTGATCCGTATCGCCCTGCCCGGCGCCCTCGCCGCGGCATTGCTCGCCAGCCAGGTC
AGCCAGGCCGCCGACCTGGTACCCCCGCCCGGCTACTACGCGGCGGTCGGCGAGCGCAAGGGCAGCGCCGGC
AGTTGCCCCGCGGTGCCGCCGCCGTATACCGGCAGCCTGGTCTTCACCAGCAAGTACGAAGGCTCCGATTCG
GCGCGGGCGACCCTCAACGTCAAGGCGGAGAAGACCTTCCGCTCGCAGATCAAGGACATCACCGACATGGAG
CGCGGCGCCACCAAGCTGGTCACCCAGTACATGCGCAGCGGCCGCGACGGCGACCTGGCCTGCGCACTGAAC
TGGATGAGCGCCTGGGCCCGCGCCGGCGCCCTGCAGAGCGACGACTTCAACCACACCGGCAAGTCCATGCGC
AAATGGGCGCTGGGCAGCCTCTCCGGCGCCTACATGCGCCTGAAGTTCTCCAGCTCGCGGCCGCTCGCGGCC
CACGCCGAGCAGAGCCGGGAAATCGAGGACTGGTTCGCCCGGCTCGGCACCCAGGTAGTCCGCGACTGGAGC
GGCCTGCCGCTGAAGAAGATCAACAACCATTCCTACTGGGCGGCCTGGTCGGTGATGTCCACCGCGGTGGTG
ACCAACCGCCGCGACCTCTTCGACTGGGCGGTGAGCGAGTTCAAGGTCGCCGCCAACCAGGTCGACGAGCAG
GGCTTCCTGCCCAACGAACTCAAGCGCCGCCAGCGCGCCCTCGCCTACCACAACTATGCGCTGCCGCCGCTG
GCGATGATCGCCGCGTTCGCCCAGGTCAACGGCGTCGACCTGCGCCAGGAGAACCACGGCGCCCTGCAGCGC
CTGGCCGAGCGGGTGATGAAGGGAGTCGACGACGAGGAAACCTTCGAGGAGAAGACCGGCGAGGACCAGGAC
ATGACCGACCTCAAGGTCGACAACAAGTACGCCTGGCTGGAGCCCTACTGCGCCCTCTACCGCTGCGAGCCG
AAGATGCTCGAGGCGAAGAAGGACCGCGAGCCGTTCAACAGTTTCCGCCTCGGCGGCGAAGTGACGCGGGTG
TTCAGCCGCGAAGGGGGAAGTTGA
Translation:
MKTSHLIRIALPGALAAALLASQVSQAADLVPPPGYYAAVGERKGSAGSCPAVPPPYTGSLVFTSKYEGSDS
ARATLNVKAEKTFRSQIKDITDMERGATKLVTQYMRSGRDGDLACALNWMSAWARAGALQSDDFNHTGKSMR
KWALGSLSGAYMRLKFSSSRPLAAHAEQSREIEDWFARLGTQVVRDWSGLPLKKINNHSYWAAWSVMSTAVV
TNRRDLFDWAVSEFKVAANQVDEQGFLPNELKRRQRALAYHNYALPPLAMIAAFAQVNGVDLRQENHGALQR
LAERVMKGVDDEETFEEKTGEDQDMTDLKVDNKYAWLEPYCALYRCEPKMLEAKKDREPFNSFRLGGEVTRV
FSREGGS*
AnnotationID:1716GeneID:1707
AnnotatorUID: danlee
Modification Date/Time: 2006-03-11 16:04:44
Gene Name:algL
Confidence Code:1
GeneProduct:poly(beta-d-mannuronate) lyase precursor AlgL
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:alginate lyase
Functional Category Confidence Code:5
COGs:cd00244,pfam05426
Secondary Functional Category(ies):Adaptation, protection; Cell wall / LPS / capsule
EC Number:4.2.2.3
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA33966.1| (AB018795) alginate lyase [Halomonas marina] [Cobetia marina]
          Length = 374

 Score =  479 bits (1232), Expect = e-134
 Identities = 230/371 (61%), Positives = 280/371 (75%), Gaps = 4/371 (1%)

>gb|AAC32313.1| (AF037600) alginate lyase [Azotobacter vinelandii]
          Length = 374

 Score =  478 bits (1229), Expect = e-134
 Identities = 228/358 (63%), Positives = 275/358 (76%), Gaps = 1/358 (0%)






 
Structural Features:
cd00244, AlgLyase, Alginate Lyase A1-III
CD-Length = 339 residues, 100.0% aligned
Score =  546 bits (1407), Expect = 2e-156
Identities = 246/339 (72%), Positives = 263/339 (77%), Gaps = 1/339 (0%)

pfam05426, Alginate_lyase, Alginate lyase.
CD-Length = 366 residues, 100.0% aligned
Score =  588 bits (1516), Expect = 5e-169
Identities = 273/366 (74%), Positives = 297/366 (81%), Gaps = 2/366 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3417




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20564




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32020




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1707_cdd.html


    		




 
URL:

















    ReferenceID:34134




  
Author/Investigator(s):
        Boyd A, Ghosh M, May TB, Shinabarger D, Keogh R, Chakrabarty AM.





  
Title:
        Sequence of the algL gene of Pseudomonas aeruginosa and purification of its alginate lyase product.




  
PubMed:
        8370530


MedLine:
        




    
Source:
Gene. 1993 Sep 6;131(1):1-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8370530

















    ReferenceID:34135




  
Author/Investigator(s):
        Monday SR, Schiller NL.





  
Title:
        Alginate synthesis in Pseudomonas aeruginosa: the role of AlgL (alginate lyase) and AlgX.




  
PubMed:
        8550492


MedLine:
        




    
Source:
J Bacteriol. 1996 Feb;178(3):625-32.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8550492

















    ReferenceID:34136




  
Author/Investigator(s):
        Schiller NL, Monday SR, Boyd CM, Keen NT, Ohman DE.





  
Title:
        Characterization of the Pseudomonas aeruginosa alginate lyase gene (algL): cloning, sequencing, and expression in Escherichia coli.




  
PubMed:
        8335634


MedLine:
        




    
Source:
J Bacteriol. 1993 Aug;175(15):4780-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8335634









           
Homologs By Global Alignment 

           

       



     
Gene ID:1707
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3001
                           PA3547_tr
                           translation of PA3547
                           367
                           99.99
                           99.99
                           0
                           1915.0
     

     
     
93315
                           gb|AAG06935.1|AE004775_6
                           (AE004775) poly(beta-d-mannuronate) lyase precursor AlgL [Pseudomonas aeruginosa PAO1]
                           367
                           99.99
                           99.99
                           0
                           1915.0
     

     
     
93316
                           gb|AAA91127.1|
                           (U27829) alginate lyase [Pseudomonas aeruginosa]
                           367
                           99.99
                           99.99
                           0
                           1915.0
     

     
     
93317
                           gb|AAA71990.1|
                           (L14597) alginate lyase [Pseudomonas aeruginosa]
                           368
                           98.09
                           98.09
                           1
                           1874.0
     

     
     
93321
                           gb|AAN66905.1|AE016319_1
                           (AE016778) alginate lyase [Pseudomonas putida KT2440]
                           373
                           62.46
                           73.45
                           8
                           1214.0
     

     






      

          
GC ORFID: 44709How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3547Glimmer Score: 

          
Start: 3368816Stop: 3369919

          
Length: 1104


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3547 translation of PA3547Homolog Bit Score 742.0

          
Other Homologs:  t_PA3547 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAACGTCCCACCTGATCCGTATCGCCCTGCCCGGCGCCCTCGCCGCGGCATTGCTCGCCAGCCAGGTC 
AGCCAGGCCGCCGACCTGGTACCCCCGCCCGGCTACTACGCGGCGGTCGGCGAGCGCAAGGGCAGCGCCGGC 
AGTTGCCCCGCGGTGCCGCCGCCGTATACCGGCAGCCTGGTCTTCACCAGCAAGTACGAAGGCTCCGATTCG 
GCGCGGGCGACCCTCAACGTCAAGGCGGAGAAGACCTTCCGCTCGCAGATCAAGGACATCACCGACATGGAG 
CGCGGCGCCACCAAGCTGGTCACCCAGTACATGCGCAGCGGCCGCGACGGCGACCTGGCCTGCGCACTGAAC 
TGGATGAGCGCCTGGGCCCGCGCCGGCGCCCTGCAGAGCGACGACTTCAACCACACCGGCAAGTCCATGCGC 
AAATGGGCGCTGGGCAGCCTCTCCGGCGCCTACATGCGCCTGAAGTTCTCCAGCTCGCGGCCGCTCGCGGCC 
CACGCCGAGCAGAGCCGGGAAATCGAGGACTGGTTCGCCCGGCTCGGCACCCAGGTAGTCCGCGACTGGAGC 
GGCCTGCCGCTGAAGAAGATCAACAACCATTCCTACTGGGCGGCCTGGTCGGTGATGTCCACCGCGGTGGTG 
ACCAACCGCCGCGACCTCTTCGACTGGGCGGTGAGCGAGTTCAAGGTCGCCGCCAACCAGGTCGACGAGCAG 
GGCTTCCTGCCCAACGAACTCAAGCGCCGCCAGCGCGCCCTCGCCTACCACAACTATGCGCTGCCGCCGCTG 
GCGATGATCGCCGCGTTCGCCCAGGTCAACGGCGTCGACCTGCGCCAGGAGAACCACGGCGCCCTGCAGCGC 
CTGGCCGAGCGGGTGATGAAGGGAGTCGACGACGAGGAAACCTTCGAGGAGAAGACCGGCGAGGACCAGGAC 
ATGACCGACCTCAAGGTCGACAACAAGTACGCCTGGCTGGAGCCCTACTGCGCCCTCTACCGCTGCGAGCCG 
AAGATGCTCGAGGCGAAGAAGGACCGCGAGCCGTTCAACAGTTTCCGCCTCGGCGGCGAAGTGACGCGGGTG 
TTCAGCCGCGAAGGGGGAAGTTGA