ViewGene
Locus Name:
GeneID:
Location:

GeneID:1700
TrivialName: PA14_66230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66230
PAO1 Ortholog Locus:PA5010
Sequence Length:1122
Protein Length:373
Start: 5901186
Stop: 5900065
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08395.1|AE004913_10 (AE004913) UDP-glucose:(heptosyl) LPS alpha 1,3-glucosyltransferase WaaG [Pseudomonas aeruginosa PAO1]
Identities = 371/373 (99%), Positives = 372/373 (99%)
Sequence:
ATGACCCTGGCGTTCATCCTCTACAAATACTTCCCCTTCGGCGGCTTGCAGCGTGACTTCATGCGCATCGCC
CTGGAATGCCAGCGGCGCGGGCACGACATCCGCGTCTATACGCTGATCTGGGAGGGCGACGTGCCGGACGGC
TTCGAAGTGCTGGTGGCCCCGGTGCGCTCGATCTTCAACCACCGGCGCAACGAGAAGTTCACCGCGTGGGTC
CGCGCCGACCTGGCCAGGCGCCCGGTGCAGCGGGTGATCGGCTTCAACAAGATGCCCGGACTGGATGTCTAC
TACGCCGCCGACGCCTGTTTCGAGGAAAAGGCCCAGACCTTGCGCAACCCGCTGTACCGCCAGTGGGGCCGC
TACCGCCACTTCGCCGGCTACGAACGGGCAGTGTTCGACCCGGCCTCGAAGACCGAGATCCTGATGATCTCC
GAGGTGCAGCAGCCGCTCTTCGTCAAGCACTACGGCACCCAGGCCGAGCGTTTCCATCTGCTGCCGCCGGGG
ATCAGCCAGGATCGCCGGGCGCCGGCCAACGCCGCGGACGTGCGTGCGGAATTCCGCCGCGAGTTCGGCCTG
GAGGAGGACGACCTGCTGCTGGTGCAGATTGGCTCCGGCTTCAAGACCAAGGGCCTGGATCGCAGCCTGAAG
GCGCTGGCCGCGCTGCCCAAGGCGTTGCGCAGGCGTACCCGGCTGATCGCCATCGGCCAGGACGATCCCAAG
CCGTTCCTGCTACAGATCGCCGCCCTCGGTCTCAACGACCAGGTACAGATCCTCAAGGGTCGCAGCGATATC
CCGCGCTTCCTGCTCGGCGCCGACCTGCTGATCCACCCGGCCTACAACGAGAACACCGGTACGGTGCTGCTG
GAGGCGCTGGTCTCCGGCCTGCCGGTGTTGGTGACCGATGTCTGCGGCTATGCCCACTACATCGCCGAGGCC
GACGCCGGGCGGGTGCTGCCGAGTCCGTTCGAGCAGGACAGTCTCAACCGCCTGCTCGCGGAAATGCTGGAG
GACGCTCCGGCGCGCGCCGCCTGGTCGCGCAATGGCCTGGCCTACGCCGATCACGCCGACCTCTACAGCATG
CCGCAGCGCGCCGCCGACCTGATCCTCGGGGAGGCCTCATGA
Translation:
MTLAFILYKYFPFGGLQRDFMRIALECQRRGHDIRVYTLIWEGDVPDGFEVLVAPVRSIFNHRRNEKFTAWV
RADLARRPVQRVIGFNKMPGLDVYYAADACFEEKAQTLRNPLYRQWGRYRHFAGYERAVFDPASKTEILMIS
EVQQPLFVKHYGTQAERFHLLPPGISQDRRAPANAADVRAEFRREFGLEEDDLLLVQIGSGFKTKGLDRSLK
ALAALPKALRRRTRLIAIGQDDPKPFLLQIAALGLNDQVQILKGRSDIPRFLLGADLLIHPAYNENTGTVLL
EALVSGLPVLVTDVCGYAHYIAEADAGRVLPSPFEQDSLNRLLAEMLEDAPARAAWSRNGLAYADHADLYSM
PQRAADLILGEAS*
AnnotationID:1709GeneID:1700
AnnotatorUID: diggins
Modification Date/Time: 2005-03-14 10:10:42
Gene Name:waaG
Confidence Code:2
GeneProduct:lipopolysaccharide core biosynthesis protein WaaG
Cell Localization:()
Synonyms:rfaG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:UDP-glucose:(heptosyl) LPS alpha 1,3-glucosyltransferase WaaG
Functional Category Confidence Code:5
COGs:pfam00534,COG0438
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65974.1|AE016226_3 (AE016775) lipopolysaccharide core biosynthesis protein WaaG
           [Pseudomonas putida KT2440]
          Length = 374

 Score =  622 bits (1603), Expect = e-177
 Identities = 303/371 (81%), Positives = 332/371 (89%)

>gb|AAO58429.1| (AE016874) lipopolysaccharide core biosynthesis protein WaaG
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 373

 Score =  604 bits (1557), Expect = e-172
 Identities = 295/371 (79%), Positives = 324/371 (87%)

>gb|AAN82891.1|AE016769_6 (AE016769) Lipopolysaccharide core biosynthesis protein rfaG
           [Escherichia coli CFT073]
          Length = 374

 Score =  397 bits (1020), Expect = e-110
 Identities = 191/370 (51%), Positives = 247/370 (66%)








 
Structural Features:
pfam00534, Glycos_transf_1, Glycosyl transferases group 1.
CD-Length = 172 residues, 100.0% aligned
Score =  113 bits (285), Expect = 3e-26
Identities = 50/173 (28%), Positives = 85/173 (49%), Gaps = 4/173 (2%)

COG0438, RfaG, Glycosyltransferase [Cell envelope biogenesis, outer membrane]
CD-Length = 381 residues,  93.7% aligned
Score = 83.6 bits (204), Expect = 4e-17







   
Genomic Context:






   
Comment:


















    ReferenceID:3403




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20550




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35050




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1700_cdd.html


    		




 
URL:

















    ReferenceID:35872




  
Author/Investigator(s):
        Parker CT, Pradel E, Schnaitman CA.





  
Title:
        Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12.




  
PubMed:
        1732225


MedLine:
        




    
Source:
J Bacteriol. 1992 Feb;174(3):930-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1732225









           
Homologs By Global Alignment 

           

       



     
Gene ID:1700
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2989
                           PA5010_tr
                           translation of PA5010
                           373
                           99.46
                           99.73
                           0
                           1922.0
     

     
     
93104
                           gb|AAG08395.1|AE004913_10
                           (AE004913) UDP-glucose:(heptosyl) LPS alpha 1,3-glucosyltransferase WaaG [Pseudomonas aeruginosa PAO1]
                           373
                           99.46
                           99.73
                           0
                           1922.0
     

     
     
93105
                           gb|AAC33168.1|
                           (U63816) glucosyltransferase I homolog [Pseudomonas aeruginosa]
                           373
                           99.46
                           99.73
                           0
                           1919.0
     

     
     
93106
                           gb|AAD33103.1|
                           (AF090724) glucosyltransferase I [Pseudomonas aeruginosa]
                           373
                           99.19
                           99.46
                           0
                           1916.0
     

     
     
93107
                           gb|AAN65974.1|AE016226_3
                           (AE016775) lipopolysaccharide core biosynthesis protein WaaG [Pseudomonas putida KT2440]
                           374
                           81.01
                           89.03
                           1
                           1603.0
     

     






      

          
GC ORFID: 44702How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5010Glimmer Score: 

          
Start: 5164869Stop: 5163748

          
Length: 1122


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5010 translation of PA5010Homolog Bit Score 744.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3380 (ORF: BLASTX 607.0), t_PA5010 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCTGGCGTTCATCCTCTACAAATACTTCCCCTTCGGCGGCTTGCAGCGTGACTTCATGCGCATCGCC 
CTGGAATGCCAGCGGCGCGGGCACGACATCCGCGTCTATACGCTGATCTGGGAGGGCGACGTGCCGGACGGC 
TTCGAAGTGCTGGTGGCCCCGGTGCGCTCGATCTTCAACCACCGGCGCAACGAGAAGTTCACCGCGTGGGTC 
CGCGCCGACCTGGCCAGGCGCCCGGTGCAGCGGGTGATCGGCTTCAACAAGATGCCCGGACTGGATGTCTAC 
TACGCCGCCGACGCCTGTTTCGAGGAAAAGGCCCAGACCTTGCGCAACCCGCTGTACCGCCAGTGGGGCCGC 
TACCGCCACTTCGCCGGCTACGAACGGGCAGTGTTCGACCCGGCCTCGAAGACCGAGATCCTGATGATCTCC 
GAGGTGCAGCAGCCGCTCTTCGTCAAGCACTACGGCACCCAGGCCGAGCGTTTCCATCTGCTGCCGCCGGGG 
ATCAGCCAGGATCGCCGGGCGCCGGCCAACGCCGCGGACGTGCGTGCGGAATTCCGCCGCGAGTTCGGCCTG 
GAGGAGGACGACCTGCTGCTGGTGCAGATTGGCTCCGGCTTCAAGACCAAGGGCCTGGATCGCAGCCTGAAG 
GCGCTGGCCGCGCTGCCCAAGGCGTTGCGCAGGCGTACCCGGCTGATCGCCATCGGCCAGGACGATCCCAAG 
CCGTTCCTGCTACAGATCGCCGCCCTCGGTCTCAACGACCAGGTACAGATCCTCAAGGGTCGCAGCGATATC 
CCGCGCTTCCTGCTCGGCGCCGACCTGCTGATCCACCCGGCCTACAACGAGAACACCGGTACGGTGCTGCTG 
GAGGCGCTGGTCTCCGGCCTGCCGGTGTTGGTGACCGATGTCTGCGGCTATGCCCACTACATCGCCGAGGCC 
GACGCCGGGCGGGTGCTGCCGAGTCCGTTCGAGCAGGACAGTCTCAACCGCCTGCTCGCGGAAATGCTGGAG 
GACGCTCCGGCGCGCGCCGCCTGGTCGCGCAATGGCCTGGCCTACGCCGATCACGCCGACCTCTACAGCATG 
CCGCAGCGCGCCGCCGACCTGATCCTCGGGGAGGCCTCATGA