ViewGene
Locus Name:
GeneID:
Location:

GeneID:1682
TrivialName: PA14_05190
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05190
PAO1 Ortholog Locus:PA0396
Sequence Length:1149
Protein Length:382
Start: 452518
Stop: 453666
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03785.1|AE004477_2 (AE004477) twitching motility protein PilU [Pseudomonas aeruginosa PAO1]
Identities = 381/382 (99%), Positives = 382/382 (100%)
Sequence:
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTA
CCGCCGTCGATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGC
GAGACCGTGCTAGGCGTGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCC
ATCAGCGCCCGCGGCATCGGCCGCTTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTG
CGCCGGATCGAGACCAACATCCCGACCCTGGAAGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTG
ACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATC
GGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGACCCGATCGAGTATATCCACCAGCAC
CAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGTGGCGCTGAAGAACACC
CTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATGCCGTGGCC
TTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTC
GCCCAGCAACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCG
CTGGCCGCCGACCTGATCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAG
GGCATGCAGACCTTCGACCAGGCGCTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTG
GCCCACGCCGACTCGGCCAACGACCTGCGCCTGATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTG
TCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACGACGACCCTGCCGGCCGGCGCTTCCGCTGA
Translation:
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFA
ISARGIGRFRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMI
GYRNKNSTGHIISIEDPIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVA
FAETGHLCLATLHANNANQALERIIHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTP
LAADLIRKGEVHELKPLMKRSTEQGMQTFDQALYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHL
SNLTQGLSLEITDDDPAGRRFR*
AnnotationID:1691GeneID:1682
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 07:07:43
Gene Name:pilU
Confidence Code:1
GeneProduct:twitching motility protein PilU
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG5008
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAB56296.1| (AJ249385) putative secretion protein [Pseudomonas stutzeri]
          Length = 381

 Score =  678 bits (1750), Expect = 0.0
 Identities = 337/382 (88%), Positives = 366/382 (95%), Gaps = 1/382 (0%)

>gb|AAM42026.1| (AE012388) twitching motility protein [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 376

 Score =  519 bits (1336), Expect = e-146
 Identities = 247/369 (66%), Positives = 307/369 (83%)

>gb|AAN56348.1|AE015772_8 (AE015772) twitching motility protein PilU [Shewanella oneidensis
           MR-1]
          Length = 370

 Score =  452 bits (1163), Expect = e-126
 Identities = 221/371 (59%), Positives = 289/371 (77%), Gaps = 2/371 (0%)








 
Structural Features:
COG5008, PilU, Tfp pilus assembly protein, ATPase PilU 
[Cell motility and secretion / Intracellular trafficking and secretion]
CD-Length = 375 residues,  98.9% aligned
Score =  638 bits (1648), Expect = 0.0
Identities = 274/371 (73%), Positives = 319/371 (85%)








   
Genomic Context:






   
Comment:


















    ReferenceID:3367




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20514




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33733




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1682_cdd.html


    		




 
URL:

















    ReferenceID:35380




  
Author/Investigator(s):
        Whitchurch CB, Mattick JS.





  
Title:
        Characterization of a gene, pilU, required for twitching motility but not phage sensitivity in Pseudomonas aeruginosa.




  
PubMed:
        7854122


MedLine:
        




    
Source:
Mol Microbiol. 1994 Sep;13(6):1079-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7854122









           
Homologs By Global Alignment 

           

       



     
Gene ID:1682
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2964
                           PA0396_tr
                           translation of PA0396
                           382
                           99.73
                           99.99
                           0
                           1937.0
     

     
     
92534
                           gb|AAG03785.1|AE004477_2
                           (AE004477) twitching motility protein PilU [Pseudomonas aeruginosa PAO1]
                           382
                           99.73
                           99.99
                           0
                           1937.0
     

     
     
92535
                           gb|AAA25965.1|
                           (L27667) pilU [Pseudomonas aeruginosa]
                           382
                           99.73
                           99.99
                           0
                           1937.0
     

     
     
92536
                           emb|CAB56296.1|
                           (AJ249385) putative secretion protein [Pseudomonas stutzeri]
                           382
                           88.21
                           95.81
                           1
                           1752.0
     

     
     
92537
                           gb|AAM42026.1|
                           (AE012388) twitching motility protein [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           386
                           64.24
                           80.05
                           14
                           1333.0
     

     






      

          
GC ORFID: 44684How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0396Glimmer Score: 

          
Start: 6253860Stop: 6255008

          
Length: 1149


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0396 translation of PA0396Homolog Bit Score 750.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6337 (ORF: BLASTX 399.0), t_PA0396 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTA 
CCGCCGTCGATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGC 
GAGACCGTGCTAGGCGTGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCC 
ATCAGCGCCCGCGGCATCGGCCGCTTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTG 
CGCCGGATCGAGACCAACATCCCGACCCTGGAAGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTG 
ACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCACCGGCAAGTCCACCTCGCTGGCGGCGATGATC 
GGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGACCCGATCGAGTATATCCACCAGCAC 
CAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGTGGCGCTGAAGAACACC 
CTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATGCCGTGGCC 
TTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC 
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTC 
GCCCAGCAACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCG 
CTGGCCGCCGACCTGATCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAG 
GGCATGCAGACCTTCGACCAGGCGCTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTG 
GCCCACGCCGACTCGGCCAACGACCTGCGCCTGATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTG 
TCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACGACGACCCTGCCGGCCGGCGCTTCCGCTGA