ViewGene
Locus Name:
GeneID:
Location:

GeneID:1673
TrivialName: PA14_57730
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57730
PAO1 Ortholog Locus:PA4444
Sequence Length:1104
Protein Length:367
Start: 5141460
Stop: 5142563
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07832.1|AE004859_1 (AE004859) soluble and membrane-bound lytic transglycosylases [Pseudomonas aeruginosa PAO1]
Identities = 364/367 (99%), Positives = 366/367 (99%)
Sequence:
ATGCGCCGTACCGCCCTCGCCCTGCCCCTGTTCCTTCTGGTTTCAGCATGCAGCAGCGAACCGACGCCACCA
CCGAAACCCGCCGCCAAACCCCAGGCCCGCACCGTCATTACCCCCCGCCCCGTACGCCAGTCGGTGCAACCG
ATACTGCCGCTGCGCGGCGATTACGCGAACAATCCGGCGGCACAGCGCTTCATCGACAGGATGGTCAGCCAG
CACGGCTTCAACCGCCAGCAACTGCACGACCTGTTCGCCCAGACCCAGCGCCTGGACTGGGTGATCCGCCTG
ATGGACCGGCAAGCTCCGACCTATACCCCACCCAGCGGACCGAACGGCGCCTGGCTGCGCTACCGGAAGAAG
TTCGTCACGCCAGGCAACGTACAGAACGGCGTGCTGTTCTGGGACCAATACGAAACCGACCTGCAACGGGCA
TCGCGCGTCTACGGCGTGCCGCCGGAGATCATCGTCGGCATCATCGGCGTGGAAACCCGCTGGGGGCGGGTG
ATGGGCAAGACGCGGATCATCGATGCGCTGTCCACCCTGTCCTTCTCCTACCCTCGCCGCGCGGAGTTCTTC
AGCGGCGAGCTGGAACAATTCCTCCTCCAGGCGCGCAAGGAAGGCACCGACCCGCTGGCCCTGCGCGGTTCC
TATGCCGGCGCCATGGGCTACGGCCAGTTCATGCCGTCTTCATTCACCAAGTACGCAGTGGACTTCGACGGC
GATGGGCATATCGACCTGTGGAATCCGCGTGACGCGATCGGCAGCGTCGCCAACTATTTCAAGCAGCACGGC
TGGGTCAACGGCGATCGCGTGGCGGTTCCCGCCAGCGGCCGGGCGCCCTCGCTGGAGGATGGCTTCAAGACG
CTGTACCCGCTGGACGTGCTCGCTTCCGCCGGACTACGCCCGCAGGGTCCGCTCGGCGGGCACCGGCAAGCC
AGCCTGCTGCGCCTGGACATGGGCAGGAACTACCAGTACTGGTACGGCTTGCCGAACTTCTACGTGATCACC
CGCTACAACCACAGCACCCACTACGCGATGGCTGTCTGGGAACTGGGCAAGGAGGTCGACCGGGTACGTCAC
CGCTCCGTCGTCAGGCAGGATTAG
Translation:
MRRTALALPLFLLVSACSSEPTPPPKPAAKPQARTVITPRPVRQSVQPILPLRGDYANNPAAQRFIDRMVSQ
HGFNRQQLHDLFAQTQRLDWVIRLMDRQAPTYTPPSGPNGAWLRYRKKFVTPGNVQNGVLFWDQYETDLQRA
SRVYGVPPEIIVGIIGVETRWGRVMGKTRIIDALSTLSFSYPRRAEFFSGELEQFLLQARKEGTDPLALRGS
YAGAMGYGQFMPSSFTKYAVDFDGDGHIDLWNPRDAIGSVANYFKQHGWVNGDRVAVPASGRAPSLEDGFKT
LYPLDVLASAGLRPQGPLGGHRQASLLRLDMGRNYQYWYGLPNFYVITRYNHSTHYAMAVWELGKEVDRVRH
RSVVRQD*
AnnotationID:1682GeneID:1673
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 21:09:40
Gene Name:mltB1
Confidence Code:1
GeneProduct:soluble and membrane-bound lytic transglycosylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2951
Secondary Functional Category(ies):
EC Number:3.2.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM85098.1|AE013755_7 (AE013755) membrane-bound lytic murein transglycosylase B [Yersinia
           pestis KIM]
          Length = 370

 Score =  521 bits (1341), Expect = e-147
 Identities = 254/362 (70%), Positives = 292/362 (80%), Gaps = 9/362 (2%)

>emb|CAC92202.1| (AJ414154) putative membrane-bound lytic murein transglycosylase B
           [Yersinia pestis CO92]
          Length = 370

 Score =  521 bits (1341), Expect = e-147
 Identities = 254/362 (70%), Positives = 292/362 (80%), Gaps = 9/362 (2%)







 
Structural Features:
COG2951, MltB, Membrane-bound lytic murein transglycosylase B 
[Cell envelope biogenesis, outer membrane]
CD-Length = 343 residues,  98.8% aligned
Score =  319 bits (818), Expect = 8e-88
Identities = 140/367 (38%), Positives = 176/367 (47%), Gaps = 34/367 (9%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3349




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20496




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31483




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1673_cdd.html


    		




 
URL:

















    ReferenceID:32832




  
Author/Investigator(s):
        Blackburn NT, Clarke AJ.





  
Title:
        Characterization of soluble and membrane-bound family 3 lytic transglycosylases from Pseudomonas aeruginosa.




  
PubMed:
        11790124


MedLine:
        




    
Source:
Biochemistry. 2002 Jan 22;41(3):1001-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11790124









           
Homologs By Global Alignment 

           

       



     
Gene ID:1673
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2950
                           PA4444_tr
                           translation of PA4444
                           367
                           99.18
                           99.72
                           0
                           1945.0
     

     
     
92295
                           gb|AAG07832.1|AE004859_1
                           (AE004859) soluble and membrane-bound lytic transglycosylases [Pseudomonas aeruginosa PAO1]
                           367
                           99.18
                           99.72
                           0
                           1945.0
     

     
     
92296
                           gb|AAM85098.1|AE013755_7
                           (AE013755) membrane-bound lytic murein transglycosylase B [Yersinia pestis KIM]
                           377
                           68.16
                           79.04
                           17
                           1351.0
     

     
     
92297
                           emb|CAC92202.1|
                           (AJ414154) putative membrane-bound lytic murein transglycosylase B [Yersinia pestis CO92]
                           377
                           68.16
                           79.04
                           17
                           1351.0
     

     
     
92302
                           dbj|BAB36981.1|
                           (AP002562) membrane-bound lytic murein transglycosylase B [Escherichia coli O157:H7]
                           373
                           65.95
                           78.28
                           18
                           1307.0
     

     






      

          
GC ORFID: 44675How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4444Glimmer Score: 

          
Start: 4405139Stop: 4406242

          
Length: 1104


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4444 translation of PA4444Homolog Bit Score 753.0

          
Other Homologs:  t_PA4001 (ORF: BLASTX 275.0), psyr_15may02_Scaffold4_revised_gene5328 (ORF: BLASTX 270.0), t_PA4444 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCCGTACCGCCCTCGCCCTGCCCCTGTTCCTTCTGGTTTCAGCATGCAGCAGCGAACCGACGCCACCA 
CCGAAACCCGCCGCCAAACCCCAGGCCCGCACCGTCATTACCCCCCGCCCCGTACGCCAGTCGGTGCAACCG 
ATACTGCCGCTGCGCGGCGATTACGCGAACAATCCGGCGGCACAGCGCTTCATCGACAGGATGGTCAGCCAG 
CACGGCTTCAACCGCCAGCAACTGCACGACCTGTTCGCCCAGACCCAGCGCCTGGACTGGGTGATCCGCCTG 
ATGGACCGGCAAGCTCCGACCTATACCCCACCCAGCGGACCGAACGGCGCCTGGCTGCGCTACCGGAAGAAG 
TTCGTCACGCCAGGCAACGTACAGAACGGCGTGCTGTTCTGGGACCAATACGAAACCGACCTGCAACGGGCA 
TCGCGCGTCTACGGCGTGCCGCCGGAGATCATCGTCGGCATCATCGGCGTGGAAACCCGCTGGGGGCGGGTG 
ATGGGCAAGACGCGGATCATCGATGCGCTGTCCACCCTGTCCTTCTCCTACCCTCGCCGCGCGGAGTTCTTC 
AGCGGCGAGCTGGAACAATTCCTCCTCCAGGCGCGCAAGGAAGGCACCGACCCGCTGGCCCTGCGCGGTTCC 
TATGCCGGCGCCATGGGCTACGGCCAGTTCATGCCGTCTTCATTCACCAAGTACGCAGTGGACTTCGACGGC 
GATGGGCATATCGACCTGTGGAATCCGCGTGACGCGATCGGCAGCGTCGCCAACTATTTCAAGCAGCACGGC 
TGGGTCAACGGCGATCGCGTGGCGGTTCCCGCCAGCGGCCGGGCGCCCTCGCTGGAGGATGGCTTCAAGACG 
CTGTACCCGCTGGACGTGCTCGCTTCCGCCGGACTACGCCCGCAGGGTCCGCTCGGCGGGCACCGGCAAGCC 
AGCCTGCTGCGCCTGGACATGGGCAGGAACTACCAGTACTGGTACGGCTTGCCGAACTTCTACGTGATCACC 
CGCTACAACCACAGCACCCACTACGCGATGGCTGTCTGGGAACTGGGCAAGGAGGTCGACCGGGTACGTCAC 
CGCTCCGTCGTCAGGCAGGATTAG