ViewGene
Locus Name:
GeneID:
Location:

GeneID:1652
TrivialName: PA14_20430
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20430
PAO1 Ortholog Locus:PA3374
Sequence Length:1164
Protein Length:387
Start: 1756434
Stop: 1757597
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06762.1|AE004759_9 (AE004759) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 387/387 (100%), Positives = 387/387 (100%)
Sequence:
ATGTCGTCTGAACGCGTTCTCAGCAATGCCCGGGTAGTCAGCGCCGAGCGGGTCTTCGTCGGCAGCGTGCTG
CTGCGCGACGGCCTGATCGCCGCGGTCGACGAAGGCCGCAGTCACCTGGCCGGGGCCGAGGACCTGGGGGGC
GACTACCTGCTGCCCGGCCTGGTCGAGCTGCACACCGACAACCTGGAGAAGCACATGACCCCGCGTCCCGGC
GTCGACTGGCCGTCGGCCTCGGCGGTGCTGGCGCACGACGCGCAGATCGTCTCCGCGGGGATCACCACGGTG
TTCGACGCACTGTCCATCGGCGACGTCAACCCCAAGGGCAAGCGCATGCAGCAGTTGCCGAAGATGCTCGAG
GCCATCGGCGAGGCCGCCGCCAGCGGCGACGCGCGCGCCGACCATCGCCTGCACCTGCGTTGCGAGGTCAGC
CATCCGCAGACGCTGGAGGTGTTCCGCGAACTGGTCGAGCATCCGCTGGTGCAACTGGTCTCGGTGATGGAC
CACGCCCCCGGCCAGCGCCAGTTCGCCAGGCTGGAGAAGTACCGCGAGTACTACCAGGGCAAGTACCACCTG
AGCGATGCCGAGATGGACGCCTTCATCGTCGAGCAGGTAGCCAACTCCGAGCGCTACAGCGACGCCGCCCGT
GCGGCCATCGTCGATGTCTGCCAGGGACGCGGCCTGTCGGTGGCCAGCCATGACGACGCGACCCTCGCGCAT
GTCCGCGAGTCGGCCGGCTACGGCATGGCCATCGCCGAGTTCCCGACCACCGTGGAGGCCGCCAGGGCCAGC
CACGAGCACGGCCTGCAGGTGCTGATGGGTGCGCCCAACGTGGTCCGCGGCGGTTCCCATTCGGGCAACGTG
GCGGCGGCGCAACTGGCGGGAATCGGCGTGCTGGATATCCTTTCCAGCGACTACTATCCGGCCAGCCTGCTG
CATGCGGCCTTGCTGCTCGCCGGCGAGCACAGCGAGTATGATGCGATCCGCTACGACCTGCCGCGCGCGGTG
GCGATGGTCAGCCGTACCCCGGCGCTGGCCGCCGGGCTGACCGATCGTGGCGAAATCCGCGTCGGCCTGCGC
GCCGATCTGCTGCAGGCGCGGGCGCTCGGCAACAACCAGCCGGTGGTGCGCCAGGTCTGGCGCGGCGGGCAG
AGAGTGTTCTGA
Translation:
MSSERVLSNARVVSAERVFVGSVLLRDGLIAAVDEGRSHLAGAEDLGGDYLLPGLVELHTDNLEKHMTPRPG
VDWPSASAVLAHDAQIVSAGITTVFDALSIGDVNPKGKRMQQLPKMLEAIGEAAASGDARADHRLHLRCEVS
HPQTLEVFRELVEHPLVQLVSVMDHAPGQRQFARLEKYREYYQGKYHLSDAEMDAFIVEQVANSERYSDAAR
AAIVDVCQGRGLSVASHDDATLAHVRESAGYGMAIAEFPTTVEAARASHEHGLQVLMGAPNVVRGGSHSGNV
AAAQLAGIGVLDILSSDYYPASLLHAALLLAGEHSEYDAIRYDLPRAVAMVSRTPALAAGLTDRGEIRVGLR
ADLLQARALGNNQPVVRQVWRGGQRVF*
AnnotationID:1661GeneID:1652
AnnotatorUID: danlee
Modification Date/Time: 2005-07-29 14:02:04
Gene Name:phnM
Confidence Code:3
GeneProduct:phosphonate metabolism protein
Cell Localization:()
Synonyms:PA3374
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:HisM-like integral membrane protein
Functional Category Confidence Code:5
COGs:COG3454
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56070.1| (AE016865) phosphonate metabolism protein PhnM [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 381

 Score =  541 bits (1394), Expect = e-153
 Identities = 275/387 (71%), Positives = 322/387 (83%), Gaps = 6/387 (1%)

>gb|AAM84310.1|AE013674_6 (AE013674) phosphonate metabolism protein [Yersinia pestis KIM]
          Length = 378

 Score =  407 bits (1046), Expect = e-113
 Identities = 214/382 (56%), Positives = 271/382 (70%), Gaps = 5/382 (1%)

>gb|AAA96994.1| (U14003) HisM-like integral membrane protein [Escherichia coli]
          Length = 378

 Score =  397 bits (1019), Expect = e-110
 Identities = 210/382 (54%), Positives = 271/382 (70%), Gaps = 5/382 (1%)






 
Structural Features:
COG3454, Metal-dependent hydrolase involved in phosphonate metabolism 
[Inorganic ion transport and metabolism]
CD-Length = 377 residues, 100.0% aligned
Score =  537 bits (1385), Expect = 9e-154
Identities = 247/383 (64%), Positives = 294/383 (76%), Gaps = 6/383 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3307




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20454




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32188




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1652_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1652
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2921
                           PA3374_tr
                           translation of PA3374
                           387
                           99.99
                           99.99
                           0
                           1957.0
     

     
     
91228
                           gb|AAG06762.1|AE004759_9
                           (AE004759) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           387
                           99.99
                           99.99
                           0
                           1957.0
     

     
     
91229
                           gb|AAO56070.1|
                           (AE016865) phosphonate metabolism protein PhnM [Pseudomonas syringae pv. tomato str. DC3000]
                           389
                           70.95
                           83.29
                           10
                           1401.0
     

     
     
91230
                           gb|AAM84310.1|AE013674_6
                           (AE013674) phosphonate metabolism protein [Yersinia pestis KIM]
                           387
                           55.29
                           70.02
                           9
                           1050.0
     

     
     
91231
                           emb|CAC92692.1|
                           (AJ414157) PhnM protein [Yersinia pestis CO92]
                           387
                           55.29
                           70.02
                           9
                           1050.0
     

     






      

          
GC ORFID: 44654How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3374Glimmer Score: 

          
Start: 3197498Stop: 3196335

          
Length: 1164


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3374 translation of PA3374Homolog Bit Score 758.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1226 (ORF: BLASTX 544.0), t_PA3374 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGTCTGAACGCGTTCTCAGCAATGCCCGGGTAGTCAGCGCCGAGCGGGTCTTCGTCGGCAGCGTGCTG 
CTGCGCGACGGCCTGATCGCCGCGGTCGACGAAGGCCGCAGTCACCTGGCCGGGGCCGAGGACCTGGGGGGC 
GACTACCTGCTGCCCGGCCTGGTCGAGCTGCACACCGACAACCTGGAGAAGCACATGACCCCGCGTCCCGGC 
GTCGACTGGCCGTCGGCCTCGGCGGTGCTGGCGCACGACGCGCAGATCGTCTCCGCGGGGATCACCACGGTG 
TTCGACGCACTGTCCATCGGCGACGTCAACCCCAAGGGCAAGCGCATGCAGCAGTTGCCGAAGATGCTCGAG 
GCCATCGGCGAGGCCGCCGCCAGCGGCGACGCGCGCGCCGACCATCGCCTGCACCTGCGTTGCGAGGTCAGC 
CATCCGCAGACGCTGGAGGTGTTCCGCGAACTGGTCGAGCATCCGCTGGTGCAACTGGTCTCGGTGATGGAC 
CACGCCCCCGGCCAGCGCCAGTTCGCCAGGCTGGAGAAGTACCGCGAGTACTACCAGGGCAAGTACCACCTG 
AGCGATGCCGAGATGGACGCCTTCATCGTCGAGCAGGTAGCCAACTCCGAGCGCTACAGCGACGCCGCCCGT 
GCGGCCATCGTCGATGTCTGCCAGGGACGCGGCCTGTCGGTGGCCAGCCATGACGACGCGACCCTCGCGCAT 
GTCCGCGAGTCGGCCGGCTACGGCATGGCCATCGCCGAGTTCCCGACCACCGTGGAGGCCGCCAGGGCCAGC 
CACGAGCACGGCCTGCAGGTGCTGATGGGTGCGCCCAACGTGGTCCGCGGCGGTTCCCATTCGGGCAACGTG 
GCGGCGGCGCAACTGGCGGGAATCGGCGTGCTGGATATCCTTTCCAGCGACTACTATCCGGCCAGCCTGCTG 
CATGCGGCCTTGCTGCTCGCCGGCGAGCACAGCGAGTATGATGCGATCCGCTACGACCTGCCGCGCGCGGTG 
GCGATGGTCAGCCGTACCCCGGCGCTGGCCGCCGGGCTGACCGATCGTGGCGAAATCCGCGTCGGCCTGCGC 
GCCGATCTGCTGCAGGCGCGGGCGCTCGGCAACAACCAGCCGGTGGTGCGCCAGGTCTGGCGCGGCGGGCAG 
AGAGTGTTCTGA