ViewGene
Locus Name:
GeneID:
Location:

GeneID:1651
TrivialName: PA14_38630
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38630
PAO1 Ortholog Locus:PA2001
Sequence Length:1182
Protein Length:393
Start: 3447122
Stop: 3445941
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05389.1|AE004626_8 (AE004626) acetyl-CoA acetyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 391/393 (99%), Positives = 392/393 (99%)
Sequence:
ATGCAAGACGTCGTCATCGTCGCCGCTACCCGTACCGCCGTCGGCAGCTTCCAGGGCTCGCTGGCCGGCATT
CCCGCCCCCGAGCTGGGCGCCGCGGTGATCCGCCGCCTGCTCGAACAGACCGGTCTCGATGCCGGCCAGGTC
GACGAAGTGATCCTCGGCCAGGTGCTCACCGCCGGGTCCGGGCAGAACCCGGCGCGCCAGGCGGCGATCAAG
GCCGGCCTGCCGGTCGGCGTGCCGGCCATGACCCTGAACAAGGTCTGCGGCTCCGGCCTCAAGGCCCTGCAC
CTGGGCGCCCAGGCGATCCGCTGCGGCGACGCCGAGGTGATCGTCGCCGGCGGCCAGGAGAACATGAGCCTG
GCGCCCTACGTCATGCCCGGCGCCCGCACCGGCCTGCGCATGGGCCATGCGAAGCTGGTCGACAGCATGATC
GAGGACGGCCTGTGGGACGCCTTCAACGACTACCACATGGGCATCACCGCCGAGAACCTGGCGGAGAAGTAC
GGCCTGACCCGCGAGGAGCAGGACGCCTTCGCCGCCGCTTCGCAGCAGAAGGCCATCGCCGCCATCGAGGGC
GGGCGCTTCCGCGACGAGATCACGCCGATCCAGGTCCCGCAGCGCAAGGGCGAGCCGCTGAGCTTCGATACC
GACGAGCAGCCGCGCGCCGGCACCACCGTCGAGGCCCTGGCCAAGCTCAAGCCGGCCTTCAGGAAGGACGGC
AGCGTCACCGCCGGCAACGCCTCCAGCCTCAACGACGGCGCCGCCGCGGTATTGCTGATGAGCGCAGCCAAG
GCCAAGGCCCTGGGCCTGCCGGTGCTGGCGCGGATCGCCAGCTACGCCAGCGCCGGGGTCGACCCGGCGATC
ATGGGCATCGGCCCGGTCTCCGCCACCCGTCGCGCGCTCGACAAGGCCGGCTGGAGCCTGGAGCAACTGGAC
CTGATCGAGGCCAACGAGGCCTTCTCCGCGCAGTCCCTGGCGGTGGGCCGGGAGCTGGGCTGGGATGCCGCC
AGGGTCAACGTCAACGGTGGCGCCATCGCCCTTGGCCACCCGATCGGCGCGTCCGGCTGCCGGGTGCTGGTG
ACCCTGCTGCACGAGATGATCCGTCGCGACGCGAAGAAGGGCCTCGCCACCCTGTGCATCGGCGGCGGGCAG
GGCGTCGCCCTGACCCTGGCCCGCGACTGA
Translation:
MQDVVIVAATRTAVGSFQGSLAGIPAPELGAAVIRRLLEQTGLDAGQVDEVILGQVLTAGSGQNPARQAAIK
AGLPVGVPAMTLNKVCGSGLKALHLGAQAIRCGDAEVIVAGGQENMSLAPYVMPGARTGLRMGHAKLVDSMI
EDGLWDAFNDYHMGITAENLAEKYGLTREEQDAFAAASQQKAIAAIEGGRFRDEITPIQVPQRKGEPLSFDT
DEQPRAGTTVEALAKLKPAFRKDGSVTAGNASSLNDGAAAVLLMSAAKAKALGLPVLARIASYASAGVDPAI
MGIGPVSATRRALDKAGWSLEQLDLIEANEAFSAQSLAVGRELGWDAARVNVNGGAIALGHPIGASGCRVLV
TLLHEMIRRDAKKGLATLCIGGGQGVALTLARD*
AnnotationID:1660GeneID:1651
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 16:04:06
Gene Name:atoB
Confidence Code:2
GeneProduct:acetyl-CoA acetyltransferase
Cell Localization:()
Synonyms:phbA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:polyhydroxybutyrate biosynthetic beta-ketothiolase
Functional Category Confidence Code:5
COGs:pfam00108,COG0183
Secondary Functional Category(ies):central intermediary metabolism
EC Number:2.3.1.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54491.1| (AE016859) acetyl-CoA acetyltransferase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 392

 Score =  652 bits (1682), Expect = 0.0
 Identities = 327/392 (83%), Positives = 359/392 (91%)

>gb|AAF28336.1|AF210312_1 (AF210312) beta-ketothiolase [Azotobacter vinelandii]
          Length = 395

 Score =  637 bits (1644), Expect = 0.0
 Identities = 318/392 (81%), Positives = 357/392 (91%)

>gb|AAN81886.1|AE016765_288 (AE016765) Probable acetyl-CoA acetyltransferase [Escherichia coli
           CFT073]
          Length = 394

 Score =  542 bits (1397), Expect = e-153
 Identities = 265/393 (67%), Positives = 333/393 (84%), Gaps = 1/393 (0%)








 
Structural Features:
pfam00108, Thiolase_N, Thiolase, N-terminal domain.
CD-Length = 264 residues, 100.0% aligned
Score =  351 bits (903), Expect = 6e-98
Identities = 171/264 (64%), Positives = 210/264 (79%), Gaps = 1/264 (0%)
 
COG0183, PaaJ, Acetyl-CoA acetyltransferase [Lipid metabolism]
CD-Length = 392 residues, 100.0% aligned
Score =  349 bits (896), Expect = 4e-97
Identities = 209/406 (51%), Positives = 253/406 (62%), Gaps = 27/406 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3305




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20452




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32725




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1651_cdd.html


    		




 
URL:

















    ReferenceID:34378




  
Author/Investigator(s):
        Jenkins LS, Nunn WD.





  
Title:
        Genetic and molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system.




  
PubMed:
        3025185


MedLine:
        




    
Source:
J Bacteriol. 1987 Jan;169(1):42-52.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3025185









           
Homologs By Global Alignment 

           

       



     
Gene ID:1651
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2913
                           PA2001_tr
                           translation of PA2001
                           393
                           99.49
                           99.74
                           0
                           1956.0
     

     
     
90874
                           gb|AAG05389.1|AE004626_8
                           (AE004626) acetyl-CoA acetyltransferase [Pseudomonas aeruginosa PAO1]
                           393
                           99.49
                           99.74
                           0
                           1956.0
     

     
     
90876
                           gb|AAO54491.1|
                           (AE016859) acetyl-CoA acetyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           393
                           83.20
                           91.34
                           1
                           1682.0
     

     
     
90878
                           gb|AAN70209.1|AE016660_6
                           (AE016791) beta-ketothiolase [Pseudomonas putida KT2440]
                           393
                           82.95
                           91.09
                           1
                           1671.0
     

     
     
90877
                           emb|CAC41637.1|
                           (AJ319748) beta-ketothiolase [Azotobacter sp. FA8]
                           393
                           82.69
                           92.11
                           1
                           1679.0
     

     






      

          
GC ORFID: 44653How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2001Glimmer Score: 

          
Start: 1506789Stop: 1507970

          
Length: 1182


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2001 translation of PA2001Homolog Bit Score 758.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2524 (ORF: BLASTX 644.0), t_PA3925 (ORF: BLASTX 362.0), t_PA2553 (ORF: BLASTX 348.0), psyr_15may02_Scaffold4_revised_gene5256 (ORF: BLASTX 327.0), t_PA0228 (ORF: BLASTX 318.0), psyr_15may02_Scaffold5_revised_gene6193 (ORF: BLASTX 310.0), t_PA3589 (ORF: BLASTX 278.0), psyr_15may02_Scaffold9_revised_gene8045 (ORF: BLASTX 268.0), t_PA3013 (ORF: BLASTX 267.0), t_PA3454 (ORF: BLASTX 258.0), t_PA2940 (ORF: BLASTX 253.0), t_PA2001 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAAGACGTCGTCATCGTCGCCGCTACCCGTACCGCCGTCGGCAGCTTCCAGGGCTCGCTGGCCGGCATT 
CCCGCCCCCGAGCTGGGCGCCGCGGTGATCCGCCGCCTGCTCGAACAGACCGGTCTCGATGCCGGCCAGGTC 
GACGAAGTGATCCTCGGCCAGGTGCTCACCGCCGGGTCCGGGCAGAACCCGGCGCGCCAGGCGGCGATCAAG 
GCCGGCCTGCCGGTCGGCGTGCCGGCCATGACCCTGAACAAGGTCTGCGGCTCCGGCCTCAAGGCCCTGCAC 
CTGGGCGCCCAGGCGATCCGCTGCGGCGACGCCGAGGTGATCGTCGCCGGCGGCCAGGAGAACATGAGCCTG 
GCGCCCTACGTCATGCCCGGCGCCCGCACCGGCCTGCGCATGGGCCATGCGAAGCTGGTCGACAGCATGATC 
GAGGACGGCCTGTGGGACGCCTTCAACGACTACCACATGGGCATCACCGCCGAGAACCTGGCGGAGAAGTAC 
GGCCTGACCCGCGAGGAGCAGGACGCCTTCGCCGCCGCTTCGCAGCAGAAGGCCATCGCCGCCATCGAGGGC 
GGGCGCTTCCGCGACGAGATCACGCCGATCCAGGTCCCGCAGCGCAAGGGCGAGCCGCTGAGCTTCGATACC 
GACGAGCAGCCGCGCGCCGGCACCACCGTCGAGGCCCTGGCCAAGCTCAAGCCGGCCTTCAGGAAGGACGGC 
AGCGTCACCGCCGGCAACGCCTCCAGCCTCAACGACGGCGCCGCCGCGGTATTGCTGATGAGCGCAGCCAAG 
GCCAAGGCCCTGGGCCTGCCGGTGCTGGCGCGGATCGCCAGCTACGCCAGCGCCGGGGTCGACCCGGCGATC 
ATGGGCATCGGCCCGGTCTCCGCCACCCGTCGCGCGCTCGACAAGGCCGGCTGGAGCCTGGAGCAACTGGAC 
CTGATCGAGGCCAACGAGGCCTTCTCCGCGCAGTCCCTGGCGGTGGGCCGGGAGCTGGGCTGGGATGCCGCC 
AGGGTCAACGTCAACGGTGGCGCCATCGCCCTTGGCCACCCGATCGGCGCGTCCGGCTGCCGGGTGCTGGTG 
ACCCTGCTGCACGAGATGATCCGTCGCGACGCGAAGAAGGGCCTCGCCACCCTGTGCATCGGCGGCGGGCAG 
GGCGTCGCCCTGACCCTGGCCCGCGACTGA