ViewGene
Locus Name:
GeneID:
Location:

GeneID:1643
TrivialName: PA14_62930
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62930
PAO1 Ortholog Locus:PA4758
Sequence Length:1137
Protein Length:378
Start: 5616832
Stop: 5615696
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08144.1|AE004889_7 (AE004889) carbamoyl-phosphate synthase small chain [Pseudomonas aeruginosa PAO1]
Identities = 377/378 (99%), Positives = 378/378 (100%)
Sequence:
TTGACTAAGCCAGCCATACTTGCACTTGCCGACGGCAGCATTTTTCGCGGTGAAGCCATCGGTGCCGACGGC
CAGACCGTTGGCGAGGTGGTGTTCAACACCGCCATGACCGGCTACCAGGAAATCCTTACCGATCCTTCCTAC
GCCCAGCAGATCGTCACCCTGACCTACCCGCACATCGGCAATACCGGTACCACCCCGGAAGACGCCGAGGCC
AACCGCGTCTGGGCCGCCGGCCTGATCATCCGCGACCTGCCGCTGATCGCCAGCAACTGGCGCAGCAAGCAG
TCGCTGCCTGACTACCTGAAAGCCAACGGCACCGTCGCCATCGCCGGCATCGATACCCGTCGCCTGACCCGC
ATCCTCCGCGAGAAGGGTTCGCAGAACGGCTGCATCCTGGCTGGTGCCGACGCTACCGAGGAACGCGCGCTG
GAGCTGGCTCGCGCCTTCCCCGGCCTGAAGGGCATGGACCTGGCCAAGGAGGTCACCACCGCCGAGCGCTAC
GAGTGGCGCTCCAGCGTCTGGAGCCTGGAAAGCGACAGTCATCCGGAGATCCCGGCCGGTGAACTGCCGTAT
CACGTGGTGGCCTACGACTACGGCGTGAAGCTGAACATCCTACGCATGCTGGTGGCGCGCGGCTGCCGCCTG
ACCGTGGTGCCGGCGCAGACACCGGCCAGCGAAGTGCTGGCGCTGAACCCTGACGGCATCTTCCTTTCCAAC
GGCCCCGGCGACCCCGAACCGTGCGACTACGCGATCCAGGCGATCCGCGAATTCCTCGATACCGAGATCCCG
GTGTTCGGCATCTGCCTCGGCCATCAGTTGCTGGCCCTGGCCTCTGGCGCCAAGACCCTGAAGATGGGCCAC
GGGCACCACGGCGCCAACCACCCGGTGCAGGACCTGGACAGCGGCGTGGTGATGATCACCAGCCAGAACCAC
GGTTTCGCGGTCGACGAGAGCACCCTGCCGGACAACCTGCGAGCCACTCACAAGTCGCTGTTCGACGGTACC
CTGCAGGGTATCGAGCGCACCGACAAGGTCGCTTTCAGCTTCCAGGGCCACCCCGAGGCGAGCCCCGGTCCG
CATGACGTCGCCCCGCTGTTCGACCGCTTCATCTCGGCGATGGCCGAGCGTCGCTGA
Translation:
MTKPAILALADGSIFRGEAIGADGQTVGEVVFNTAMTGYQEILTDPSYAQQIVTLTYPHIGNTGTTPEDAEA
NRVWAAGLIIRDLPLIASNWRSKQSLPDYLKANGTVAIAGIDTRRLTRILREKGSQNGCILAGADATEERAL
ELARAFPGLKGMDLAKEVTTAERYEWRSSVWSLESDSHPEIPAGELPYHVVAYDYGVKLNILRMLVARGCRL
TVVPAQTPASEVLALNPDGIFLSNGPGDPEPCDYAIQAIREFLDTEIPVFGICLGHQLLALASGAKTLKMGH
GHHGANHPVQDLDSGVVMITSQNHGFAVDESTLPDNLRATHKSLFDGTLQGIERTDKVAFSFQGHPEASPGP
HDVAPLFDRFISAMAERR*
AnnotationID:1652GeneID:1643
AnnotatorUID: diggins
Modification Date/Time: 2005-04-27 07:07:28
Gene Name:carA
Confidence Code:1
GeneProduct:carbamoyl-phosphate synthase small chain
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0505,pfam00988
Secondary Functional Category(ies):amino acid biosynthesis and metabolism
EC Number:6.3.5.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70296.1|AE016670_6 (AE016791) carbamoyl-phosphate synthase, small subunit [Pseudomonas
           putida KT2440]
          Length = 378

 Score =  681 bits (1757), Expect = 0.0
 Identities = 331/378 (87%), Positives = 356/378 (94%)

>gb|AAA19049.1| (U04993) carbamoyl phosphate synthetase light subunit [Pseudomonas
           stutzeri]
          Length = 384

 Score =  673 bits (1737), Expect = 0.0
 Identities = 326/374 (87%), Positives = 355/374 (94%)

>gb|AAN78538.1|AE016755_38 (AE016755) Carbamoyl-phosphate synthase small chain [Escherichia
           coli CFT073]
          Length = 391

 Score =  548 bits (1411), Expect = e-155
 Identities = 269/378 (71%), Positives = 306/378 (80%)






 
Structural Features:
COG0505, CarA, Carbamoylphosphate synthase small subunit 
[Amino acid transport and metabolism / Nucleotide transport and metabolism]
CD-Length = 368 residues,  99.2% aligned
Score =  588 bits (1518), Expect = 3e-169
Identities = 226/376 (60%), Positives = 263/376 (69%), Gaps = 11/376 (2%)

pfam00988, CPSase_sm_chain, Carbamoyl-phosphate synthase small chain, 
CPSase domain. 
CD-Length = 148 residues, 100.0% aligned
Score =  220 bits (561), Expect = 3e-58
Identities = 82/149 (55%), Positives = 100/149 (67%), Gaps = 1/149 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14403




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20436




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37661




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1643_cdd.html


    		




 
URL:

















    ReferenceID:37718




  
Author/Investigator(s):
        Kwon DH, Lu CD, Walthall DA, Brown TM, Houghton JE, Abdelal AT.





  
Title:
        Structure and regulation of the carAB operon in Pseudomonas aeruginosa and Pseudomonas stutzeri: no untranslated region exists.




  
PubMed:
        8169201


MedLine:
        




    
Source:
J Bacteriol. 1994 May;176(9):2532-42.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8169201









           
Homologs By Global Alignment 

           

       



     
Gene ID:1643
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2905
                           PA4758_tr
                           translation of PA4758
                           378
                           99.73
                           99.99
                           0
                           1962.0
     

     
     
90600
                           gb|AAG08144.1|AE004889_7
                           (AE004889) carbamoyl-phosphate synthase small chain [Pseudomonas aeruginosa PAO1]
                           378
                           99.47
                           99.99
                           0
                           1960.0
     

     
     
90601
                           gb|AAB39250.1|
                           (U81259) carbamoylphosphate synthetase small subunit [Pseudomonas aeruginosa]
                           378
                           99.47
                           99.99
                           0
                           1960.0
     

     
     
90602
                           gb|AAA19046.1|
                           (U04992) carbamoyl phosphate synthetase light subunit [Pseudomonas aeruginosa]
                           378
                           99.47
                           99.99
                           0
                           1960.0
     

     
     
90603
                           gb|AAN70296.1|AE016670_6
                           (AE016791) carbamoyl-phosphate synthase, small subunit [Pseudomonas putida KT2440]
                           378
                           87.30
                           94.17
                           0
                           1754.0
     

     






      

          
GC ORFID: 44645How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4758Glimmer Score: 

          
Start: 4880512Stop: 4879376

          
Length: 1137


          
Start Codon: TTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4758 translation of PA4758Homolog Bit Score 760.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5480 (ORF: BLASTX 674.0), t_PA4758 (ORF: GLIMMER)

          
GC ORF Sequence

           
TTGACTAAGCCAGCCATACTTGCACTTGCCGACGGCAGCATTTTTCGCGGTGAAGCCATCGGTGCCGACGGC 
CAGACCGTTGGCGAGGTGGTGTTCAACACCGCCATGACCGGCTACCAGGAAATCCTTACCGATCCTTCCTAC 
GCCCAGCAGATCGTCACCCTGACCTACCCGCACATCGGCAATACCGGTACCACCCCGGAAGACGCCGAGGCC 
AACCGCGTCTGGGCCGCCGGCCTGATCATCCGCGACCTGCCGCTGATCGCCAGCAACTGGCGCAGCAAGCAG 
TCGCTGCCTGACTACCTGAAAGCCAACGGCACCGTCGCCATCGCCGGCATCGATACCCGTCGCCTGACCCGC 
ATCCTCCGCGAGAAGGGTTCGCAGAACGGCTGCATCCTGGCTGGTGCCGACGCTACCGAGGAACGCGCGCTG 
GAGCTGGCTCGCGCCTTCCCCGGCCTGAAGGGCATGGACCTGGCCAAGGAGGTCACCACCGCCGAGCGCTAC 
GAGTGGCGCTCCAGCGTCTGGAGCCTGGAAAGCGACAGTCATCCGGAGATCCCGGCCGGTGAACTGCCGTAT 
CACGTGGTGGCCTACGACTACGGCGTGAAGCTGAACATCCTACGCATGCTGGTGGCGCGCGGCTGCCGCCTG 
ACCGTGGTGCCGGCGCAGACACCGGCCAGCGAAGTGCTGGCGCTGAACCCTGACGGCATCTTCCTTTCCAAC 
GGCCCCGGCGACCCCGAACCGTGCGACTACGCGATCCAGGCGATCCGCGAATTCCTCGATACCGAGATCCCG 
GTGTTCGGCATCTGCCTCGGCCATCAGTTGCTGGCCCTGGCCTCTGGCGCCAAGACCCTGAAGATGGGCCAC 
GGGCACCACGGCGCCAACCACCCGGTGCAGGACCTGGACAGCGGCGTGGTGATGATCACCAGCCAGAACCAC 
GGTTTCGCGGTCGACGAGAGCACCCTGCCGGACAACCTGCGAGCCACTCACAAGTCGCTGTTCGACGGTACC 
CTGCAGGGTATCGAGCGCACCGACAAGGTCGCTTTCAGCTTCCAGGGCCACCCCGAGGCGAGCCCCGGTCCG 
CATGACGTCGCCCCGCTGTTCGACCGCTTCATCTCGGCGATGGCCGAGCGTCGCTGA