ViewGene
Locus Name:
GeneID:
Location:

GeneID:1632
TrivialName: PA14_03940
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03940
PAO1 Ortholog Locus:PA0302
Sequence Length:1155
Protein Length:384
Start: 355236
Stop: 356390
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03691.1|AE004468_3 (AE004468) polyamine transport protein PotG [Pseudomonas aeruginosa PAO1]
Identities = 384/384 (100%), Positives = 384/384 (100%)
Sequence:
ATGGCGATAGCCTCCGGTGCCTACAAGAAAGCCCTAGAGGGCAACCAGCAGCCGAAAGAGGTGCTGGTAAAG
ATCGACCGGGTAACCAAGCAGTTCGACGAGACGCTGGCGGTGGACACCGTGTCCCTGACCATCAACAAGGGC
GAGATCTTCGCCCTGCTCGGCGGCTCCGGCTCGGGTAAATCCACCCTGTTGCGCATGCTCGCCGGTTTCGAG
CGGCCCACCGAGGGGCGGATCTTCCTCGATGGCCAGGACATCACCGACCTGCCGCCCTACGAGCGACCGATC
AACATGATGTTCCAGTCCTATGCGCTGTTCCCGCACATGAGCGTGGCGCAGAACATCGCCTTCGGCCTCAAG
CAGGACGGCCTGCCCAAGGCCGAGATCGACGAGCGCGTGGCCGAGATGCTCAAGCTGGTGCACATGACCCAG
TACGCCAAGCGCAAGCCGCACCAGCTCTCCGGCGGCCAGCGCCAGCGGGTGGCCCTGGCCCGTTCGCTGGCC
AAGCGGCCGAAGCTGCTGCTGCTCGACGAGCCGATGGGCGCGCTGGACAAGAAGCTGCGCTCGCAGATGCAA
CTGGAACTGGTGGAGATCATCGAGCGCGTCGGCGTGACCTGCGTGATGGTGACCCACGACCAGGAAGAGGCC
ATGACCATGGCCCAGCGCATCGCCATCATGCACCTGGGCTGCATCGAGCAGATCGGCAGCCCGATGGACATC
TACGAGACCCCGGCGAGCCGCCTGGTCTGCGAGTTCATCGGCAACGTCAACCTGTTCGACGGCGTGCTGGTG
GAGGACGTGCAGGACCATGCGGTGATCGCCTGCCCGCAGCTGGAGAACCCGATCTACATCGGCCACGGCATC
AGCACCCGCGCCGAGGACAAGCGCATCACCTACGCGCTGCGGCCGGAGAAAGTGATGATCAGCGCGCAGAAG
CCGGACAACCTGGAACACGCCGGGCACAACTGGGCCCAGGGCACCGTCTACGACATCGCCTACCTTGGCGGC
CACTCGGTGTACTACATCAAGCTGGCCTCCGGGATGATCGTCCAGGCCTTCATGGCCAACGCCGAGCGCCAC
GTCGCCCGGCCGACCTGGGACCAGTCGGTGTACATCAGCTGGTACGACGACAGCGGCGTGGTACTGCAGTCA
TGA
Translation:
MAIASGAYKKALEGNQQPKEVLVKIDRVTKQFDETLAVDTVSLTINKGEIFALLGGSGSGKSTLLRMLAGFE
RPTEGRIFLDGQDITDLPPYERPINMMFQSYALFPHMSVAQNIAFGLKQDGLPKAEIDERVAEMLKLVHMTQ
YAKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMGALDKKLRSQMQLELVEIIERVGVTCVMVTHDQEEA
MTMAQRIAIMHLGCIEQIGSPMDIYETPASRLVCEFIGNVNLFDGVLVEDVQDHAVIACPQLENPIYIGHGI
STRAEDKRITYALRPEKVMISAQKPDNLEHAGHNWAQGTVYDIAYLGGHSVYYIKLASGMIVQAFMANAERH
VARPTWDQSVYISWYDDSGVVLQS*
AnnotationID:1641GeneID:1632
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 07:07:35
Gene Name:spuF
Confidence Code:1
GeneProduct:polyamine transport protein PotG
Cell Localization:()
Synonyms:potG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:putrescine ABC transporter, ATP-binding protein
Functional Category Confidence Code:5
COGs:COG3842
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70744.1|AE016718_3 (AE016793) putrescine ABC transporter, ATP-binding protein
           [Pseudomonas putida KT2440]
          Length = 380

 Score =  625 bits (1612), Expect = e-178
 Identities = 310/384 (80%), Positives = 346/384 (90%), Gaps = 4/384 (1%)

>gb|AAO58729.1| (AE016875) putrescine ABC transporter, ATP-binding protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 380

 Score =  615 bits (1585), Expect = e-175
 Identities = 306/384 (79%), Positives = 345/384 (89%), Gaps = 4/384 (1%)

>gb|AAN54339.1|AE015571_2 (AE015571) polyamine ABC transporter, ATP-binding protein
           [Shewanella oneidensis MR-1]
          Length = 378

 Score =  530 bits (1365), Expect = e-150
 Identities = 273/384 (71%), Positives = 311/384 (80%), Gaps = 6/384 (1%)








 
Structural Features:
COG3842, PotA, ABC-type spermidine/putrescine transport systems, 
ATPase components 
[Amino acid transport and metabolism]
CD-Length = 352 residues,  99.1% aligned
Score =  403 bits (1038), Expect = 1e-113
Identities = 172/365 (47%), Positives = 230/365 (63%), Gaps = 17/365 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3267




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20414




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33655




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1632_cdd.html


    		




 
URL:

















    ReferenceID:35429




  
Author/Investigator(s):
        Lu CD, Itoh Y, Nakada Y, Jiang Y.





  
Title:
        Functional analysis and regulation of the divergent spuABCDEFGH-spuI operons for polyamine uptake and utilization in Pseudomonas aeruginosa PAO1.




  
PubMed:
        12081945


MedLine:
        




    
Source:
J Bacteriol. 2002 Jul;184(14):3765-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12081945









           
Homologs By Global Alignment 

           

       



     
Gene ID:1632
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2877
                           PA0302_tr
                           translation of PA0302
                           384
                           99.99
                           99.99
                           0
                           1970.0
     

     
     
89715
                           gb|AAG03691.1|AE004468_3
                           (AE004468) polyamine transport protein PotG [Pseudomonas aeruginosa PAO1]
                           384
                           99.99
                           99.99
                           0
                           1970.0
     

     
     
89716
                           gb|AAN70744.1|AE016718_3
                           (AE016793) putrescine ABC transporter, ATP-binding protein [Pseudomonas putida KT2440]
                           384
                           80.72
                           90.10
                           4
                           1615.0
     

     
     
89717
                           gb|AAO58729.1|
                           (AE016875) putrescine ABC transporter, ATP-binding protein [Pseudomonas syringae pv. tomato str. DC3000]
                           384
                           79.94
                           90.10
                           4
                           1589.0
     

     
     
89718
                           gb|AAK15489.1|AF323694_4
                           (AF323694) PotG [Pseudomonas fluorescens]
                           384
                           77.86
                           88.80
                           4
                           1566.0
     

     






      

          
GC ORFID: 44634How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0302Glimmer Score: 

          
Start: 6156578Stop: 6157732

          
Length: 1155


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0302 translation of PA0302Homolog Bit Score 763.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6513 (ORF: BLASTX 611.0), t_PA3607 (ORF: BLASTX 285.0), t_PA0326 (ORF: BLASTX 272.0), t_PA0603 (ORF: BLASTX 268.0), t_PA0206 (ORF: BLASTX 265.0), psyr_15may02_Scaffold7_revised_gene6963 (ORF: BLASTX 264.0), psyr_15may02_Scaffold13_revised_gene2134 (ORF: BLASTX 263.0), psyr_15may02_Scaffold7_revised_gene6993 (ORF: BLASTX 236.0), psyr_15may02_Scaffold3_revised_gene4421 (ORF: BLASTX 221.0), t_PA3187 (ORF: BLASTX 217.0), psyr_15may02_Scaffold4_revised_gene5887 (ORF: BLASTX 213.0), psyr_15may02_Scaffold2_revised_gene3068 (ORF: BLASTX 207.0), t_PA0302 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGATAGCCTCCGGTGCCTACAAGAAAGCCCTAGAGGGCAACCAGCAGCCGAAAGAGGTGCTGGTAAAG 
ATCGACCGGGTAACCAAGCAGTTCGACGAGACGCTGGCGGTGGACACCGTGTCCCTGACCATCAACAAGGGC 
GAGATCTTCGCCCTGCTCGGCGGCTCCGGCTCGGGTAAATCCACCCTGTTGCGCATGCTCGCCGGTTTCGAG 
CGGCCCACCGAGGGGCGGATCTTCCTCGATGGCCAGGACATCACCGACCTGCCGCCCTACGAGCGACCGATC 
AACATGATGTTCCAGTCCTATGCGCTGTTCCCGCACATGAGCGTGGCGCAGAACATCGCCTTCGGCCTCAAG 
CAGGACGGCCTGCCCAAGGCCGAGATCGACGAGCGCGTGGCCGAGATGCTCAAGCTGGTGCACATGACCCAG 
TACGCCAAGCGCAAGCCGCACCAGCTCTCCGGCGGCCAGCGCCAGCGGGTGGCCCTGGCCCGTTCGCTGGCC 
AAGCGGCCGAAGCTGCTGCTGCTCGACGAGCCGATGGGCGCGCTGGACAAGAAGCTGCGCTCGCAGATGCAA 
CTGGAACTGGTGGAGATCATCGAGCGCGTCGGCGTGACCTGCGTGATGGTGACCCACGACCAGGAAGAGGCC 
ATGACCATGGCCCAGCGCATCGCCATCATGCACCTGGGCTGCATCGAGCAGATCGGCAGCCCGATGGACATC 
TACGAGACCCCGGCGAGCCGCCTGGTCTGCGAGTTCATCGGCAACGTCAACCTGTTCGACGGCGTGCTGGTG 
GAGGACGTGCAGGACCATGCGGTGATCGCCTGCCCGCAGCTGGAGAACCCGATCTACATCGGCCACGGCATC 
AGCACCCGCGCCGAGGACAAGCGCATCACCTACGCGCTGCGGCCGGAGAAAGTGATGATCAGCGCGCAGAAG 
CCGGACAACCTGGAACACGCCGGGCACAACTGGGCCCAGGGCACCGTCTACGACATCGCCTACCTTGGCGGC 
CACTCGGTGTACTACATCAAGCTGGCCTCCGGGATGATCGTCCAGGCCTTCATGGCCAACGCCGAGCGCCAC 
GTCGCCCGGCCGACCTGGGACCAGTCGGTGTACATCAGCTGGTACGACGACAGCGGCGTGGTACTGCAGTCA 
TGA