ViewGene
Locus Name:
GeneID:
Location:

GeneID:1622
TrivialName: PA14_14600
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_14600
PAO1 Ortholog Locus:PA3823
Sequence Length:1119
Protein Length:372
Start: 1245985
Stop: 1247103
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07210.1|AE004799_16 (AE004799) queuine tRNA-ribosyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 372/372 (100%), Positives = 372/372 (100%)
Sequence:
ATGAACTTCGAGCTGCTGGCGACCGACGGCAAGGCCCGTCGCGGCCGCCTGACCTTCCCCCGCGGCGTGGTT
GAGACCCCGGCCTTCATGCCGGTGGGCACCTACGGCACGGTGAAGGGCATGTTGCCGCGCGACATCGAGGAC
ATCGGCGCGCAGATCATCCTCGGCAACACCTTCCATCTCTGGTTGCGCCCGGGCACCGAGGTGATCCAGCGC
CACGGCGACCTGCACGACTTCATGCAGTGGAAAGGCCCGATCCTCACCGATTCCGGCGGTTTCCAGGTGTTC
AGCCTGGGAGCCATGCGCAAGATCAAGGAGGAGGGCGTGACCTTCGCCTCGCCGGTGGACGGCGCCAAGGTC
TTCATGGGGCCGGAGGAGTCCATGGCGGTGCAACGCGCCCTGGGCTCGGACATCGTGATGATCTTCGACGAA
TGCACGCCGTACCCGGCCGACCATGACGTCGCCAAGCGCTCCATGGAGCTGTCGCTGCGCTGGGCCAAGCGC
TCGAAGATCGCCCATGGCGATAGCCCGTCGGCGCTGTTCGGCATCGTCCAGGGCGGTATGCACGAGGACCTG
CGCCTGCGCTCGCTGGATGGCTTGCAGGAGATCGGCTTCGACGGCCTGGCCATCGGTGGTTTGTCGGTGGGC
GAGCCGAAGGAAGAGATGATCCGCGTGCTCGATTTCCTGCCGCCGCAGATGCCGGCCGACAAGCCGCGCTAC
CTGATGGGCGTGGGCAAGCCGGAAGACCTGGTGGAAGGCGTGCGCCGCGGCGTGGACATGTTCGACTGTGTG
ATGCCGACGCGCAATGCGCGCAACGGCCACCTGTTCGTCGACAGCGGGGTGATCAAGATCCGCAACTCGGTA
CACAAGCACGACGACTCCACGCTCGACCCGACCTGCGACTGTTACACCTGCAAACATTTTTCCCGCGCCTAT
CTGCACCACTTGGACAAGTGCGGGGAAATGCTCGGTAGCATGCTCAATACCATCCACAACTTGCGGCATTAT
CAGCGGGTTATGGCTGGTTTGCGCGAGGCAATCCAACAGGGTACATTGGCCGCCTTTGTCGATGCCTTCTAT
GCCAAGCGCGGCCTTCCCACGCCGCCGCTGGATGCCTGA
Translation:
MNFELLATDGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPRDIEDIGAQIILGNTFHLWLRPGTEVIQR
HGDLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGAKVFMGPEESMAVQRALGSDIVMIFDE
CTPYPADHDVAKRSMELSLRWAKRSKIAHGDSPSALFGIVQGGMHEDLRLRSLDGLQEIGFDGLAIGGLSVG
EPKEEMIRVLDFLPPQMPADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFVDSGVIKIRNSV
HKHDDSTLDPTCDCYTCKHFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQRVMAGLREAIQQGTLAAFVDAFY
AKRGLPTPPLDA*
AnnotationID:1631GeneID:1622
AnnotatorUID: diggins
Modification Date/Time: 2005-04-24 15:03:44
Gene Name:tgt
Confidence Code:2
GeneProduct:queuine tRNA-ribosyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0343,KOG3908,pfam01702
Secondary Functional Category(ies):
EC Number:2.4.2.29
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54934.1| (AE016860) queuine tRNA-ribosyltransferase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 371

 Score =  692 bits (1787), Expect = 0.0
 Identities = 332/370 (89%), Positives = 354/370 (95%)

>gb|AAN78994.1|AE016756_177 (AE016756) Queuine tRNA-ribosyltransferase [Escherichia coli
           CFT073]
          Length = 375

 Score =  556 bits (1432), Expect = e-158
 Identities = 263/373 (70%), Positives = 313/373 (83%), Gaps = 2/373 (0%)

>gb|AAN56119.1|AE015748_7 (AE015748) queuine tRNA-ribosyltransferase [Shewanella oneidensis
           MR-1]
          Length = 374

 Score =  545 bits (1405), Expect = e-154
 Identities = 258/372 (69%), Positives = 307/372 (82%), Gaps = 2/372 (0%)






 
Structural Features:
COG0343, Tgt, Queuine/archaeosine tRNA-ribosyltransferase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 372 residues,  99.2% aligned
Score =  557 bits (1436), Expect = 2e-159
Identities = 224/369 (60%), Positives = 270/369 (73%), Gaps = 1/369 (0%)

KOG3908, Queuine-tRNA ribosyltransferase 
[RNA processing and modification]
CD-Length = 396 residues,  92.2% aligned
Score =  359 bits (922), Expect = 8e-100
Identities = 167/366 (45%), Positives = 227/366 (62%), Gaps = 4/366 (1%)

pfam01702, TGT, Queuine tRNA-ribosyltransferase.
CD-Length = 238 residues,  99.6% aligned
Score =  326 bits (836), Expect = 7e-90
Identities = 129/237 (54%), Positives = 164/237 (69%), Gaps = 1/237 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3247




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20394




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31734




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1622_cdd.html


    		




 
URL:

















    ReferenceID:34311




  
Author/Investigator(s):
        Bereswill S, Fassbinder F, Volzing C, Haas R, Reuter K, Ficner R, Kist M.





  
Title:
        Cloning and functional characterization of the genes encoding 3-dehydroquinate synthase (aroB) and tRNA-guanine transglycosylase (tgt) from Helicobacter pylori.




  
PubMed:
        9403840


MedLine:
        




    
Source:
Med Microbiol Immunol (Berl). 1997 Oct;186(2-3):125-34.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9403840

















    ReferenceID:34312




  
Author/Investigator(s):
        Reuter K, Ficner R.





  
Title:
        Sequence analysis and overexpression of the Zymomonas mobilis tgt gene encoding tRNA-guanine transglycosylase: purification and biochemical characterization of the enzyme.




  
PubMed:
        7665516


MedLine:
        




    
Source:
J Bacteriol. 1995 Sep;177(18):5284-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7665516

















    ReferenceID:34313




  
Author/Investigator(s):
        Durand JM, Okada N, Tobe T, Watarai M, Fukuda I, Suzuki T, Nakata N, Komatsu K, Yoshikawa M, Sasakawa C.





  
Title:
        vacC, a virulence-associated chromosomal locus of Shigella flexneri, is homologous to tgt, a gene encoding tRNA-guanine transglycosylase (Tgt) of Escherichia coli K-12.




  
PubMed:
        8045893


MedLine:
        




    
Source:
J Bacteriol. 1994 Aug;176(15):4627-34.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8045893

















    ReferenceID:34314




  
Author/Investigator(s):
        Reuter K, Slany R, Ullrich F, Kersten H.





  
Title:
        Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes.




  
PubMed:
        1706703


MedLine:
        




    
Source:
J Bacteriol. 1991 Apr;173(7):2256-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1706703









           
Homologs By Global Alignment 

           

       



     
Gene ID:1622
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2865
                           PA3823_tr
                           translation of PA3823
                           372
                           99.99
                           99.99
                           0
                           1974.0
     

     
     
89398
                           gb|AAG07210.1|AE004799_16
                           (AE004799) queuine tRNA-ribosyltransferase [Pseudomonas aeruginosa PAO1]
                           372
                           99.99
                           99.99
                           0
                           1974.0
     

     
     
89399
                           gb|AAO54934.1|
                           (AE016860) queuine tRNA-ribosyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           372
                           89.24
                           95.16
                           1
                           1786.0
     

     
     
89400
                           gb|AAN66458.1|AE016275_3
                           (AE016777) queuine tRNA-ribosyltransferase [Pseudomonas putida KT2440]
                           372
                           87.63
                           94.62
                           1
                           1767.0
     

     
     
89401
                           gb|AAM84572.1|AE013703_2
                           (AE013703) tRNA-guanine transglycosylase [Yersinia pestis KIM]
                           374
                           71.65
                           85.02
                           2
                           1470.0
     

     






      

          
GC ORFID: 44624How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3823Glimmer Score: 

          
Start: 3707953Stop: 3706835

          
Length: 1119


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3823 translation of PA3823Homolog Bit Score 764.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4508 (ORF: BLASTX 691.0), t_PA3823 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACTTCGAGCTGCTGGCGACCGACGGCAAGGCCCGTCGCGGCCGCCTGACCTTCCCCCGCGGCGTGGTT 
GAGACCCCGGCCTTCATGCCGGTGGGCACCTACGGCACGGTGAAGGGCATGTTGCCGCGCGACATCGAGGAC 
ATCGGCGCGCAGATCATCCTCGGCAACACCTTCCATCTCTGGTTGCGCCCGGGCACCGAGGTGATCCAGCGC 
CACGGCGACCTGCACGACTTCATGCAGTGGAAAGGCCCGATCCTCACCGATTCCGGCGGTTTCCAGGTGTTC 
AGCCTGGGAGCCATGCGCAAGATCAAGGAGGAGGGCGTGACCTTCGCCTCGCCGGTGGACGGCGCCAAGGTC 
TTCATGGGGCCGGAGGAGTCCATGGCGGTGCAACGCGCCCTGGGCTCGGACATCGTGATGATCTTCGACGAA 
TGCACGCCGTACCCGGCCGACCATGACGTCGCCAAGCGCTCCATGGAGCTGTCGCTGCGCTGGGCCAAGCGC 
TCGAAGATCGCCCATGGCGATAGCCCGTCGGCGCTGTTCGGCATCGTCCAGGGCGGTATGCACGAGGACCTG 
CGCCTGCGCTCGCTGGATGGCTTGCAGGAGATCGGCTTCGACGGCCTGGCCATCGGTGGTTTGTCGGTGGGC 
GAGCCGAAGGAAGAGATGATCCGCGTGCTCGATTTCCTGCCGCCGCAGATGCCGGCCGACAAGCCGCGCTAC 
CTGATGGGCGTGGGCAAGCCGGAAGACCTGGTGGAAGGCGTGCGCCGCGGCGTGGACATGTTCGACTGTGTG 
ATGCCGACGCGCAATGCGCGCAACGGCCACCTGTTCGTCGACAGCGGGGTGATCAAGATCCGCAACTCGGTA 
CACAAGCACGACGACTCCACGCTCGACCCGACCTGCGACTGTTACACCTGCAAACATTTTTCCCGCGCCTAT 
CTGCACCACTTGGACAAGTGCGGGGAAATGCTCGGTAGCATGCTCAATACCATCCACAACTTGCGGCATTAT 
CAGCGGGTTATGGCTGGTTTGCGCGAGGCAATCCAACAGGGTACATTGGCCGCCTTTGTCGATGCCTTCTAT 
GCCAAGCGCGGCCTTCCCACGCCGCCGCTGGATGCCTGA