ViewGene
Locus Name:
GeneID:
Location:

GeneID:1620
TrivialName: PA14_17130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17130
PAO1 Ortholog Locus:PA3650
Sequence Length:1191
Protein Length:396
Start: 1467925
Stop: 1469115
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07038.1|AE004785_2 (AE004785) 1-deoxy-d-xylulose 5-phosphate reductoisomerase [Pseudomonas aeruginosa PAO1]
Identities = 395/396 (99%), Positives = 395/396 (99%)
Sequence:
ATGAGTCGACCGCAGCGGATCAGCGTGCTCGGCGCGACCGGCTCGATCGGCCTGAGCACCCTGGACGTCGTC
CAGCGTCATCCCGATCGTTACGAAGCCTTCGCCCTGACCGGCTTCAGCCGCCTGGCCGAACTCGAggCGCTG
TGCCTCAgGCACCGCCCCGTCTATGCGGTGGTGCCGGAGCaggCCGCGGCGATCGCCTTGCAGGGCTCGCTC
GCCGCGGCGGGTATCCGCACCCGGGTGCTGTTCGGCGAGCAGGCGTTGTGCGAAGTGGCCAGCGCGCCCGAA
GTGGACATGGTAATGGCGGCCATCGTCGGCGCCGCCGGGCTGCCGTCGACCCTGGCGGCCGTCGAGGCCGGC
AAGCGCGTACTGCTGGCCAACAAGGAAGCGCTGGTGATGTCCGGCGCGCTGTTCATGCAGGCGGTCAAGCGC
AGCGGCGCGGTGCTCCTGCCGATCGACAGCGAGCACAACGCGATCTTCCAGTCGCTGCCGCGCAATTATGCC
GATGGCCTGGAGCGGGTCGGCGTGCGCCGGATCCTCTTGACCGCCTCCGGCGGCCCGTTCCGCGAGACGCCG
CTGGAGCAACTCGCTTCGGTGACGCCGGAGCAGGCTTGTGCGCACCCGAACTGGTCGATGGGGCGGAAGATT
TCCGTCGATTCCGCCAGCATGATGAACAAGGGGCTCGAACTGATCGAGGCGTGCTGGCTGTTCGACGCCCAG
CCGAGCCAGGTCGAGGTGGTGATCCACCCGCAGAGCGTGATCCACTCGATGGTGGACTACGTCGACGGCTCG
GTGATCGCCCAGCTCGGCAATCCGGACATGCGCACGCCGATTTCCTATGCCATGGCCTGGCCGGAGCGAATC
GATTCCGGCGTTTCGCCGCTGGACATGTTCGCCGTCGGTCGCCTGGATTTCCAGCGCCCCGACGAGCAGCGC
TTCCCCTGCCTGCGCCTGGCGAGCCAGGCCGCGGAAACCGGCGGCAGCGCCCCGGCCATGCTGAATGCCGCG
AACGAGGTGGCCGTGGCCGCATTTCTCGAGCGGCGCATCCGCTTCAGCGACATCGCGGTTATCATCGAGGAC
GTGCTGAACCGCGAGGCGGTGACCGCAGTCGAATCGCTCGATCAGGTCCTGGCCGCCGATCGCCGCGCCCGT
TCGGTCGCCGGGCAATGGTTGACCCGACACGCCGGCTAG
Translation:
MSRPQRISVLGATGSIGLSTLDVVQRHPDRYEAFALTGFSRLAELEALCLRHRPVYAVVPEQAAAIALQGSL
AAAGIRTRVLFGEQALCEVASAPEVDMVMAAIVGAAGLPSTLAAVEAGKRVLLANKEALVMSGALFMQAVKR
SGAVLLPIDSEHNAIFQSLPRNYADGLERVGVRRILLTASGGPFRETPLEQLASVTPEQACAHPNWSMGRKI
SVDSASMMNKGLELIEACWLFDAQPSQVEVVIHPQSVIHSMVDYVDGSVIAQLGNPDMRTPISYAMAWPERI
DSGVSPLDMFAVGRLDFQRPDEQRFPCLRLASQAAETGGSAPAMLNAANEVAVAAFLERRIRFSDIAVIIED
VLNREAVTAVESLDQVLAADRRARSVAGQWLTRHAG*
AnnotationID:1629GeneID:1620
AnnotatorUID: diggins
Modification Date/Time: 2005-06-19 19:07:46
Gene Name:dxr
Confidence Code:2
GeneProduct:1-deoxy-d-xylulose 5-phosphate reductoisomerase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0743,pfam02670
Secondary Functional Category(ies):
EC Number:1.1.1.267
Status:ACTIVE
Pathway:
Isoprenoid biosynthesis; 
Isopentenyl-PP biosynthesis via DXP pathway; 
Isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 1.
Homology:
>gb|AAN67218.1|AE016348_10 (AE016779) 1-deoxy-D-xylulose 5-phosphate reductoisomerase
           [Pseudomonas putida KT2440]
          Length = 404

 Score =  610 bits (1574), Expect = e-174
 Identities = 309/394 (78%), Positives = 346/394 (87%)

>dbj|BAA32426.1| (AB013300) 1-deoxy-D-xylulose 5-phosphate reductoisomerase
           [Escherichia coli]
          Length = 398

 Score =  435 bits (1118), Expect = e-121
 Identities = 230/397 (57%), Positives = 288/397 (72%), Gaps = 7/397 (1%)
Structural Features:
COG0743, Dxr, 1-deoxy-D-xylulose 5-phosphate reductoisomerase 
[Lipid metabolism]
CD-Length = 385 residues, 100.0% aligned
Score =  550 bits (1419), Expect = 2e-157
Identities = 225/394 (57%), Positives = 278/394 (70%), Gaps = 10/394 (2%)

pfam02670, DXP_reductoisom, 1-deoxy-D-xylulose 5-phosphate reductoisomerase.
CD-Length = 372 residues, 100.0% aligned
Score =  455 bits (1171), Expect = 1e-128
Identities = 195/381 (51%), Positives = 249/381 (65%), Gaps = 9/381 (2%)
Genomic Context:






   
Comment:


















    ReferenceID:3243




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20390




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31893




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1620_cdd.html


    		




 
URL:

















    ReferenceID:34216




  
Author/Investigator(s):
        Carretero-Paulet L, Ahumada I, Cunillera N, Rodriguez-Concepcion M, Ferrer A, Boronat A, Campos N.





  
Title:
        Expression and molecular analysis of the Arabidopsis DXR gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, the first committed enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway.




  
PubMed:
        12177470


MedLine:
        




    
Source:
Plant Physiol. 2002 Aug;129(4):1581-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12177470

















    ReferenceID:34217




  
Author/Investigator(s):
        Miller B, Heuser T, Zimmer W.





  
Title:
        Functional involvement of a deoxy-D-xylulose 5-phosphate reductoisomerase gene harboring locus of Synechococcus leopoliensis in isoprenoid biosynthesis.




  
PubMed:
        11007968


MedLine:
        




    
Source:
FEBS Lett. 2000 Sep 22;481(3):221-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11007968









           
Homologs By Global Alignment 

           

       



     
Gene ID:1620
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2863
                           PA3650_tr
                           translation of PA3650
                           396
                           99.74
                           99.74
                           0
                           1972.0
     

     
     
89305
                           gb|AAG07038.1|AE004785_2
                           (AE004785) 1-deoxy-d-xylulose 5-phosphate reductoisomerase [Pseudomonas aeruginosa PAO1]
                           396
                           99.74
                           99.74
                           0
                           1972.0
     

     
     
89306
                           gb|AAF97241.1|AF282879_1
                           (AF282879) 1-deoxy-D-xylulose 5-phosphate reductoisomerase; Dxp reductoisomerase; Dxr [Pseudomonas aeruginosa]
                           396
                           99.74
                           99.74
                           0
                           1972.0
     

     
     
89308
                           gb|AAO55060.1|
                           (AE016861) 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Pseudomonas syringae pv. tomato str. DC3000]
                           396
                           78.78
                           86.86
                           0
                           1565.0
     

     
     
89307
                           gb|AAN67218.1|AE016348_10
                           (AE016779) 1-deoxy-D-xylulose 5-phosphate reductoisomerase [Pseudomonas putida KT2440]
                           404
                           76.48
                           85.64
                           8
                           1572.0
     

     






      

          
GC ORFID: 44622How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3650Glimmer Score: 

          
Start: 3486012Stop: 3484822

          
Length: 1191


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA3650 translation of PA3650Homolog Bit Score 764.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4625 (ORF: BLASTX 604.0), t_PA3650 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTCGACCGCAGCGGATCAGCGTGCTCGGCGCGACCGGCTCGATCGGCCTGAGCACCCTGGACGTCGTC 
CAGCGTCATCCCGATCGTTACGAAGCCTTCGCCCTGACCGGCTTCAGCCGCCTGGCCGAACTCGAggCGCTG 
TGCCTCAgGCACCGCCCCGTCTATGCGGTGGTGCCGGAGCaggCCGCGGCGATCGCCTTGCAGGGCTCGCTC 
GCCGCGGCGGGTATCCGCACCCGGGTGCTGTTCGGCGAGCAGGCGTTGTGCGAAGTGGCCAGCGCGCCCGAA 
GTGGACATGGTAATGGCGGCCATCGTCGGCGCCGCCGGGCTGCCGTCGACCCTGGCGGCCGTCGAGGCCGGC 
AAGCGCGTACTGCTGGCCAACAAGGAAGCGCTGGTGATGTCCGGCGCGCTGTTCATGCAGGCGGTCAAGCGC 
AGCGGCGCGGTGCTCCTGCCGATCGACAGCGAGCACAACGCGATCTTCCAGTCGCTGCCGCGCAATTATGCC 
GATGGCCTGGAGCGGGTCGGCGTGCGCCGGATCCTCTTGACCGCCTCCGGCGGCCCGTTCCGCGAGACGCCG 
CTGGAGCAACTCGCTTCGGTGACGCCGGAGCAGGCTTGTGCGCACCCGAACTGGTCGATGGGGCGGAAGATT 
TCCGTCGATTCCGCCAGCATGATGAACAAGGGGCTCGAACTGATCGAGGCGTGCTGGCTGTTCGACGCCCAG 
CCGAGCCAGGTCGAGGTGGTGATCCACCCGCAGAGCGTGATCCACTCGATGGTGGACTACGTCGACGGCTCG 
GTGATCGCCCAGCTCGGCAATCCGGACATGCGCACGCCGATTTCCTATGCCATGGCCTGGCCGGAGCGAATC 
GATTCCGGCGTTTCGCCGCTGGACATGTTCGCCGTCGGTCGCCTGGATTTCCAGCGCCCCGACGAGCAGCGC 
TTCCCCTGCCTGCGCCTGGCGAGCCAGGCCGCGGAAACCGGCGGCAGCGCCCCGGCCATGCTGAATGCCGCG 
AACGAGGTGGCCGTGGCCGCATTTCTCGAGCGGCGCATCCGCTTCAGCGACATCGCGGTTATCATCGAGGAC 
GTGCTGAACCGCGAGGCGGTGACCGCAGTCGAATCGCTCGATCAGGTCCTGGCCGCCGATCGCCGCGCCCGT 
TCGGTCGCCGGGCAATGGTTGACCCGACACGCCGGCTAG