ViewGene
Locus Name:
GeneID:
Location:

GeneID:1610
TrivialName: PA14_30150
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30150
PAO1 Ortholog Locus:PA2626
Sequence Length:1128
Protein Length:375
Start: 2611923
Stop: 2610796
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06014.1|AE004691_5 (AE004691) tRNA methyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 375/375 (100%), Positives = 375/375 (100%)
Sequence:
ATGTCTGAATCCGCCCCCACCCCACGCCGCGAGCGCGTGATCGTCGGCATGTCCGGCGGCGTCGACTCGTCC
GTCTCCGCCCTGCTCCTGTTGCAGCAGGGCTATCAGGTCGAAGGCCTGTTCATGAAGAACTGGGACGAGGAC
GACGGCACCGAATACTGTACCGCCCGCGAGGACCTGGCCGACGCCCAGGCCGTCTGCGACCGGATCGGCATC
AAGCTGCACACGGCGAACTTCGCCGCGGAATACTGGGATAACGTGTTCGAGCACTTCCTTGCCGAGTACAAG
GCCGGCCGTACGCCGAACCCGGACATCCTCTGCAACCGCGAAATCAAGTTCAAGGCGTTCCTCGACTACGCG
CTGATGCTCGGCGCGGACCTGATCGCCACCGGCCACTATGTGCGGCGCCGCGACCGCGACGGGCGCACCGAG
CTGCTCAAGGGCCTGGACCCGAACAAGGATCAGAGCTACTTCCTGCACGCGGTGGGCGGCGAGCAGATCGCC
CGCAGCCTGTTCCCCGTCGGCGAACTGGAGAAGCCGGAAGTCCGCGCCATCGCCGAGAAGCATGGCCTCGCC
ACGGCGAAGAAGAAGGACTCCACGGGCATCTGCTTCATTGGCGAGCGACGCTTCACCGATTTCCTCAAGCAG
TACCTGCCGGCCCAGCCGGGCGACATCGAGACCACCGAAGGCAAGGTCATCGGTCGCCACAGCGGCCTGATG
TACCACACCATCGGCCAGCGCCAGGGCCTCGGCATCGGCGGTCTCAAGGAAGCCGGCGACGATCCCTGGTAC
GTGCTCGGCAAGGACCTGCAACGCAACGTGCTGCTGGTCGGCCAGGGCAACGACCACCCGCTGCTGTTCTCC
CGCGCCCTGCTGGCCTCGCGAATCTACTGGGTCAACCCGGTCGAACTGGAGCGGCCGCGCCGCCTGCGGGCC
AAGGTGCGCTACCGGCAGAGCGACCAGGACTGCGTCCTGGAGAAAACCGCCGAAGGCTACCGCGCGGTGTTC
GACGAACCGCAGCGCGCAGTGACCCCGGGGCAATCGGTGGTGTTCTACGACGGCGACGTCTGCCTCGGCGGC
GGCGTGATCGAAACGGCGGAAGCCTGGGATTTCGGAGGACGCCCGTGA
Translation:
MSESAPTPRRERVIVGMSGGVDSSVSALLLLQQGYQVEGLFMKNWDEDDGTEYCTAREDLADAQAVCDRIGI
KLHTANFAAEYWDNVFEHFLAEYKAGRTPNPDILCNREIKFKAFLDYALMLGADLIATGHYVRRRDRDGRTE
LLKGLDPNKDQSYFLHAVGGEQIARSLFPVGELEKPEVRAIAEKHGLATAKKKDSTGICFIGERRFTDFLKQ
YLPAQPGDIETTEGKVIGRHSGLMYHTIGQRQGLGIGGLKEAGDDPWYVLGKDLQRNVLLVGQGNDHPLLFS
RALLASRIYWVNPVELERPRRLRAKVRYRQSDQDCVLEKTAEGYRAVFDEPQRAVTPGQSVVFYDGDVCLGG
GVIETAEAWDFGGRP*
AnnotationID:1619GeneID:1610
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:17
Gene Name:trmU
Confidence Code:2
GeneProduct:tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
Cell Localization:()
Synonyms:mnmA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(24) Transcription, RNA processing and degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0482,pfam03054
Secondary Functional Category(ies):
EC Number:2.1.1.61
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56836.1| (AE016868) tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 376

 Score =  672 bits (1733), Expect = 0.0
 Identities = 324/370 (87%), Positives = 346/370 (93%), Gaps = 1/370 (0%)

>gb|AAN69608.1|AE016594_5 (AE016788) tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Pseudomonas putida KT2440]
          Length = 374

 Score =  667 bits (1720), Expect = 0.0
 Identities = 323/368 (87%), Positives = 342/368 (92%)

>gb|AAF94287.1| (AE004193) tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Vibrio cholerae]
          Length = 372

 Score =  511 bits (1315), Expect = e-144
 Identities = 251/368 (68%), Positives = 296/368 (80%), Gaps = 4/368 (1%)






 
Structural Features:
COG0482, TrmU, Predicted tRNA(5-methylaminomethyl-2-thiouridylate) 
methyltransferase, contains the PP-loop ATPase domain 
[Translation, ribosomal structure and biogenesis]
CD-Length = 356 residues, 100.0% aligned
Score =  509 bits (1311), Expect = 3e-145
Identities = 218/363 (60%), Positives = 273/363 (75%), Gaps = 10/363 (2%)

pfam03054, tRNA_Me_trans, tRNA methyl transferase. 
CD-Length = 355 residues, 100.0% aligned
Score =  480 bits (1238), Expect = 1e-136
Identities = 206/359 (57%), Positives = 252/359 (70%), Gaps = 10/359 (2%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3223




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20370




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33193




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1610_cdd.html


    		




 
URL:

















    ReferenceID:34570




  
Author/Investigator(s):
        Kambampati R, Lauhon CT.





  
Title:
        MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli.




  
PubMed:
        12549933


MedLine:
        




    
Source:
Biochemistry. 2003 Feb 4;42(4):1109-17.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12549933









           
Homologs By Global Alignment 

           

       



     
Gene ID:1610
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2851
                           PA2626_tr
                           translation of PA2626
                           375
                           99.99
                           99.99
                           0
                           1981.0
     

     
     
88829
                           gb|AAG06014.1|AE004691_5
                           (AE004691) tRNA methyltransferase [Pseudomonas aeruginosa PAO1]
                           375
                           99.99
                           99.99
                           0
                           1981.0
     

     
     
88830
                           gb|AAO56836.1|
                           (AE016868) tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           376
                           86.17
                           92.28
                           1
                           1729.0
     

     
     
88831
                           gb|AAN69608.1|AE016594_5
                           (AE016788) tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Pseudomonas putida KT2440]
                           378
                           85.71
                           90.74
                           7
                           1714.0
     

     
     
88834
                           gb|AAF94287.1|
                           (AE004193) tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [Vibrio cholerae O1 biovar eltor str. N16961]
                           380
                           66.31
                           78.68
                           13
                           1317.0
     

     






      

          
GC ORFID: 44612How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2626Glimmer Score: 

          
Start: 2342003Stop: 2343130

          
Length: 1128


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2626 translation of PA2626Homolog Bit Score 767.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2756 (ORF: BLASTX 672.0), t_PA2626 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTGAATCCGCCCCCACCCCACGCCGCGAGCGCGTGATCGTCGGCATGTCCGGCGGCGTCGACTCGTCC 
GTCTCCGCCCTGCTCCTGTTGCAGCAGGGCTATCAGGTCGAAGGCCTGTTCATGAAGAACTGGGACGAGGAC 
GACGGCACCGAATACTGTACCGCCCGCGAGGACCTGGCCGACGCCCAGGCCGTCTGCGACCGGATCGGCATC 
AAGCTGCACACGGCGAACTTCGCCGCGGAATACTGGGATAACGTGTTCGAGCACTTCCTTGCCGAGTACAAG 
GCCGGCCGTACGCCGAACCCGGACATCCTCTGCAACCGCGAAATCAAGTTCAAGGCGTTCCTCGACTACGCG 
CTGATGCTCGGCGCGGACCTGATCGCCACCGGCCACTATGTGCGGCGCCGCGACCGCGACGGGCGCACCGAG 
CTGCTCAAGGGCCTGGACCCGAACAAGGATCAGAGCTACTTCCTGCACGCGGTGGGCGGCGAGCAGATCGCC 
CGCAGCCTGTTCCCCGTCGGCGAACTGGAGAAGCCGGAAGTCCGCGCCATCGCCGAGAAGCATGGCCTCGCC 
ACGGCGAAGAAGAAGGACTCCACGGGCATCTGCTTCATTGGCGAGCGACGCTTCACCGATTTCCTCAAGCAG 
TACCTGCCGGCCCAGCCGGGCGACATCGAGACCACCGAAGGCAAGGTCATCGGTCGCCACAGCGGCCTGATG 
TACCACACCATCGGCCAGCGCCAGGGCCTCGGCATCGGCGGTCTCAAGGAAGCCGGCGACGATCCCTGGTAC 
GTGCTCGGCAAGGACCTGCAACGCAACGTGCTGCTGGTCGGCCAGGGCAACGACCACCCGCTGCTGTTCTCC 
CGCGCCCTGCTGGCCTCGCGAATCTACTGGGTCAACCCGGTCGAACTGGAGCGGCCGCGCCGCCTGCGGGCC 
AAGGTGCGCTACCGGCAGAGCGACCAGGACTGCGTCCTGGAGAAAACCGCCGAAGGCTACCGCGCGGTGTTC 
GACGAACCGCAGCGCGCAGTGACCCCGGGGCAATCGGTGGTGTTCTACGACGGCGACGTCTGCCTCGGCGGC 
GGCGTGATCGAAACGGCGGAAGCCTGGGATTTCGGAGGACGCCCGTGA