ViewGene
Locus Name:
GeneID:
Location:

GeneID:1602
TrivialName: PA14_31530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_31530
PAO1 Ortholog Locus:PA2553
Sequence Length:1191
Protein Length:396
Start: 2740838
Stop: 2742028
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05941.1|AE004683_5 (AE004683) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
Identities = 395/396 (99%), Positives = 395/396 (99%)
Sequence:
ATGACCCGCGAAGATCCGATCGTCATCGTCAGCGCCGTCCGCACCCCGATGGGCGGCTTCCTCGGCGACTTC
AAGGACGTCAACGCCGCTACCCTCGGCGCCGCGGCGGTGCGCGCCGCGGTGGAGCGGGCGCGCCTGCAAGCC
GACGAAGTGGACGAGGCCGTGCTCGGCTGCGTGCTCGCCGCCGgCcAGGGCCagGCGCCGGCGCGCCAGGCG
GTGCTTGGCGCCGGGCTGGCGCGCGGCACGCCGTGCTCGACGCTGAACAAGATGTGTGGCTCGGGCATGAAG
GCGCTGATGCTCGCCCACGACACGTTGCTCGCCGGCAGCGCCGGGGTCGCGCTGGCCGGGGGCATGGAAAGC
ATGTCCAACGCGCCCTACCTGCTGGAGCGCGCCCGTAGCGGCTACCGCATGGGCCACGGCAAGGTGCTCGAC
CACATGTTCCTCGACGGCCTCGAAGACGCCTACGACAAGGGCCGCCTGATGGGCACCTTCGCCGAGGACTGC
GCCGAGGCCTACGGCTTCACCCGCGAAGCACAGGACGAGTTCGCCGTGGCCTCGCTGACCCGCGCGCAGCAA
GCCATGCGCGACGGGCGCTTCCAGGCCGAGATCGTGCCGCTGACGGTCACCGCCGGCAAGACCGAGCGCCTG
GTGGATAGCGACGAGCAGCCGCCGAAGGCCCGGCTGGACAAGATCCCGACCCTGAAGCCGGCGTTCCGCGAA
GGCGGCACGGTAACCGCGGCCAACTCCAGTTCGATTTCCGACGGCGCCGCCGCGCTGGTCCTGATGCGCCTT
TCCGAGGCCGAGCGACGCGGGCTCGCGCCGTTGGCGGCGATTCGTGGGCATGCTTCGTTCGCCGACGCGCCG
AACCTGTTCCCCACCGCGCCGGTCGGTGCGGTGAAGCGCCTGATGCAGCGTACCGGCTGGTCGCTGGGCGAG
GTCGACCTGTTCGAGGTCAACGAAGCCTTCGCCGTGGTCGGCATGGCGGCAATGCGCGACCTCGACCTGTCC
CACGAGCACCTCAACGTCCACGGCGGCGCCTGTGCGCTGGGCCATCCGATCGGCGCGTCCGGCGCGCGGATC
GTCGTGACCCTGCTCAACGCCCTGCAGCAGTACGACCTGGAACGCGGCGTGGCGGCGGTCTGCATCGGCGGC
GGCGAGGCGACCGCGATCGCCGTCGAGCGCTTGCGCTGA
Translation:
MTREDPIVIVSAVRTPMGGFLGDFKDVNAATLGAAAVRAAVERARLQADEVDEAVLGCVLAAGQGQAPARQA
VLGAGLARGTPCSTLNKMCGSGMKALMLAHDTLLAGSAGVALAGGMESMSNAPYLLERARSGYRMGHGKVLD
HMFLDGLEDAYDKGRLMGTFAEDCAEAYGFTREAQDEFAVASLTRAQQAMRDGRFQAEIVPLTVTAGKTERL
VDSDEQPPKARLDKIPTLKPAFREGGTVTAANSSSISDGAAALVLMRLSEAERRGLAPLAAIRGHASFADAP
NLFPTAPVGAVKRLMQRTGWSLGEVDLFEVNEAFAVVGMAAMRDLDLSHEHLNVHGGACALGHPIGASGARI
VVTLLNALQQYDLERGVAAVCIGGGEATAIAVERLR*
AnnotationID:1611GeneID:1602
AnnotatorUID: diggins
Modification Date/Time: 2005-03-06 22:10:34
Gene Name:
Confidence Code:3
GeneProduct:putative acyl-CoA thiolase
Cell Localization:()
Synonyms:PA2553
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:putative acetyl-CoA acetyltransferase
Functional Category Confidence Code:5
COGs:COG0183,pfam00108,pfam02803
Secondary Functional Category(ies):putative enzyme
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67828.1|AE016414_3 (AE016782) 3-ketoacyl-CoA thiolase [Pseudomonas putida KT2440]
          Length = 397

 Score =  590 bits (1521), Expect = e-168
 Identities = 290/391 (74%), Positives = 341/391 (87%)

>gb|AAN33642.1|AE014543_6 (AE014543) acetyl-CoA acetyltransferase [Brucella suis 1330]
          Length = 396

 Score =  571 bits (1471), Expect = e-162
 Identities = 281/395 (71%), Positives = 332/395 (84%)

>gb|AAK65449.1| (AE007266) Probable thiolase [Sinorhizobium meliloti]
          Length = 396

 Score =  565 bits (1455), Expect = e-160
 Identities = 278/393 (70%), Positives = 330/393 (83%)






 
Structural Features:
COG0183, PaaJ, Acetyl-CoA acetyltransferase [Lipid metabolism]
CD-Length = 392 residues,  99.7% aligned
Score =  280 bits (718), Expect = 2e-76
Identities = 176/405 (43%), Positives = 221/405 (54%), Gaps = 28/405 (6%)

pfam00108, Thiolase_N, Thiolase, N-terminal domain.
CD-Length = 264 residues,  98.9% aligned
Score =  270 bits (693), Expect = 1e-73
Identities = 135/262 (51%), Positives = 174/262 (66%), Gaps = 4/262 (1%)

pfam02803, Thiolase_C, Thiolase, C-terminal domain.
CD-Length = 123 residues, 100.0% aligned
Score =  130 bits (329), Expect = 2e-31
Identities = 58/123 (47%), Positives = 85/123 (69%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3207




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20354




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33130




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1602_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1602
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2836
                           PA2553_tr
                           translation of PA2553
                           396
                           99.74
                           99.74
                           0
                           1984.0
     

     
     
88442
                           gb|AAG05941.1|AE004683_5
                           (AE004683) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
                           396
                           99.74
                           99.74
                           0
                           1984.0
     

     
     
88443
                           gb|AAN67828.1|AE016414_3
                           (AE016782) 3-ketoacyl-CoA thiolase [Pseudomonas putida KT2440]
                           397
                           73.55
                           86.39
                           1
                           1518.0
     

     
     
88445
                           emb|CAE27745.1|
                           (BX572600) putative acyl-CoA thiolase [Rhodopseudomonas palustris CGA009]
                           396
                           72.72
                           85.10
                           1
                           1495.0
     

     
     
88444
                           gb|AAK18171.1|AF290950_3
                           (AF290950) FadAx [Pseudomonas putida]
                           397
                           72.54
                           85.13
                           1
                           1496.0
     

     






      

          
GC ORFID: 44604How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2553Glimmer Score: 

          
Start: 2213088Stop: 2211898

          
Length: 1191


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2553 translation of PA2553Homolog Bit Score 768.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5256 (ORF: BLASTX 572.0), t_PA3925 (ORF: BLASTX 476.0), t_PA2001 (ORF: BLASTX 347.0), psyr_15may02_Scaffold15_revised_gene2524 (ORF: BLASTX 341.0), t_PA0228 (ORF: BLASTX 260.0), t_PA2553 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCGCGAAGATCCGATCGTCATCGTCAGCGCCGTCCGCACCCCGATGGGCGGCTTCCTCGGCGACTTC 
AAGGACGTCAACGCCGCTACCCTCGGCGCCGCGGCGGTGCGCGCCGCGGTGGAGCGGGCGCGCCTGCAAGCC 
GACGAAGTGGACGAGGCCGTGCTCGGCTGCGTGCTCGCCGCCGgCcAGGGCCagGCGCCGGCGCGCCAGGCG 
GTGCTTGGCGCCGGGCTGGCGCGCGGCACGCCGTGCTCGACGCTGAACAAGATGTGTGGCTCGGGCATGAAG 
GCGCTGATGCTCGCCCACGACACGTTGCTCGCCGGCAGCGCCGGGGTCGCGCTGGCCGGGGGCATGGAAAGC 
ATGTCCAACGCGCCCTACCTGCTGGAGCGCGCCCGTAGCGGCTACCGCATGGGCCACGGCAAGGTGCTCGAC 
CACATGTTCCTCGACGGCCTCGAAGACGCCTACGACAAGGGCCGCCTGATGGGCACCTTCGCCGAGGACTGC 
GCCGAGGCCTACGGCTTCACCCGCGAAGCACAGGACGAGTTCGCCGTGGCCTCGCTGACCCGCGCGCAGCAA 
GCCATGCGCGACGGGCGCTTCCAGGCCGAGATCGTGCCGCTGACGGTCACCGCCGGCAAGACCGAGCGCCTG 
GTGGATAGCGACGAGCAGCCGCCGAAGGCCCGGCTGGACAAGATCCCGACCCTGAAGCCGGCGTTCCGCGAA 
GGCGGCACGGTAACCGCGGCCAACTCCAGTTCGATTTCCGACGGCGCCGCCGCGCTGGTCCTGATGCGCCTT 
TCCGAGGCCGAGCGACGCGGGCTCGCGCCGTTGGCGGCGATTCGTGGGCATGCTTCGTTCGCCGACGCGCCG 
AACCTGTTCCCCACCGCGCCGGTCGGTGCGGTGAAGCGCCTGATGCAGCGTACCGGCTGGTCGCTGGGCGAG 
GTCGACCTGTTCGAGGTCAACGAAGCCTTCGCCGTGGTCGGCATGGCGGCAATGCGCGACCTCGACCTGTCC 
CACGAGCACCTCAACGTCCACGGCGGCGCCTGTGCGCTGGGCCATCCGATCGGCGCGTCCGGCGCGCGGATC 
GTCGTGACCCTGCTCAACGCCCTGCAGCAGTACGACCTGGAACGCGGCGTGGCGGCGGTCTGCATCGGCGGC 
GGCGAGGCGACCGCGATCGCCGTCGAGCGCTTGCGCTGA