ViewGene
Locus Name:
GeneID:
Location:

GeneID:1593
TrivialName: PA14_73040
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_73040
PAO1 Ortholog Locus:PA5538
Sequence Length:1194
Protein Length:397
Start: 6504807
Stop: 6503614
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08923.1|AE004966_5 (AE004966) N-acetylmuramoyl-L-alanine amidase [Pseudomonas aeruginosa PAO1]
Identities = 396/397 (99%), Positives = 397/397 (100%)
Sequence:
ATGAAGCGCCGTCGCCTCCTGCAAAGCCTGCTCGCCGGCCTGGCCCTCCAGCCGTTCCTGGCCGCCAGCGCC
AATGTACGAATCCGCCAGGCCCGCGCCTGGAATTCCTCGGAAAGCTGGCGCCTGGTGCTGGAACTGGACGGT
CCGCCGCGTTATCGAACCTTTTCCCTGCAGGCCCCCGAGCGGTTGATCCTCGACCTGCCCGACGCGCAATTG
CTCGCAACGCTCTCCGAGCTGCCGCTGGACGGCCCCGTGCGCGCCATCCGCAGCGGCCAACTGGGTGGCGGC
GGGACGCGCATCGTCCTCGACCTGCGCCAGGCGGTCAGGCCGCGCAGCTTCGCCCTGGAACCGCAGGCAGGA
AAGGGCCATCGCCTGGTCCTGGACCTTTATCCACAGGCGCTGGCGGCTCCCCCCGAGCCGATGCTGGCGCTG
GCCCGCCCGCTACCCTCGACGGGCCGCGACATCCTCGTGGTGGTCGATGCCGGCCATGGCGGCAAGGACCCC
GGCGCGGTCGGTAGCAAGGGCGAGCGCGAAAAGGACGTGGCGCTGGCCATCGCCCGCCTGCTGGCGCGGCGG
ATCGACCGCGAGAAAGGCTTCAAGGCGCGCCTGGTGCGCAACGGCGACGTGTTCGTCCCGCTGCGCAAGCGG
CCGGAAGTGGCTCGGCGCTACAACGCCGACATGTTCGTCTCGGTGCACGCCGACGCGGCGCCGCGACTGACC
GCCTCCGGCGCATCGGTATATGCCCTCTCCGAGCATGGCGCCAGCTCGACCATGGCGCGCTGGATGGCCGAC
CGCGAGAACGGCGCCGACCTGATCGGCAACAGCGGCAACCTGCCGCTACAGGGCCGCGACAAGGTACTCGCC
GGGGTGATCCTCGACATGGCCCTGAGTTCGACCATCGCCAGCAGCCTGGACCTCGGCCACAGCGTTCTCGGC
CAGTTGTCGGACGTCACCGCGCTGCACCAGAAGCGTGTCGAGCAGGCCGGCTTCGCCGTGCTCAAGTCGCCG
GACATCCCTTCGATCCTGGTGGAGACCGGCTTCATCTCGAATCGCGGCGATTGTCGCCGGTTGATGGACGAA
CGCCATCGCCAGCGCCTGGCCGAGGCGATCTTCGCTGGCGTGCAGGGCTACTTCCGGCAACGCCCTCCCGCC
GGCAGCTACCTGGCGGCGCTCAGCGCGCAGCCTCCCGGCTAG
Translation:
MKRRRLLQSLLAGLALQPFLAASANVRIRQARAWNSSESWRLVLELDGPPRYRTFSLQAPERLILDLPDAQL
LATLSELPLDGPVRAIRSGQLGGGGTRIVLDLRQAVRPRSFALEPQAGKGHRLVLDLYPQALAAPPEPMLAL
ARPLPSTGRDILVVVDAGHGGKDPGAVGSKGEREKDVALAIARLLARRIDREKGFKARLVRNGDVFVPLRKR
PEVARRYNADMFVSVHADAAPRLTASGASVYALSEHGASSTMARWMADRENGADLIGNSGNLPLQGRDKVLA
GVILDMALSSTIASSLDLGHSVLGQLSDVTALHQKRVEQAGFAVLKSPDIPSILVETGFISNRGDCRRLMDE
RHRQRLAEAIFAGVQGYFRQRPPAGSYLAALSAQPPG*
AnnotationID:1602GeneID:1593
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 07:07:31
Gene Name:amiA
Confidence Code:2
GeneProduct:N-acetylmuramoyl-L-alanine amidase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0860
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:3.5.1.28
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58947.1| (AE016876) N-acetylmuramoyl-L-alanine amidase, family 3
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 407

 Score =  434 bits (1116), Expect = e-121
 Identities = 239/403 (59%), Positives = 299/403 (74%), Gaps = 14/403 (3%)

>gb|AAN70464.1|AE016688_3 (AE016792) N-acetylmuramoyl-L-alanine amidase [Pseudomonas putida
           KT2440]
          Length = 476

 Score =  380 bits (976), Expect = e-105
 Identities = 198/414 (47%), Positives = 280/414 (67%), Gaps = 26/414 (6%)






 
Structural Features:
COG0860, AmiC, N-acetylmuramoyl-L-alanine amidase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 231 residues,  99.6% aligned
Score =  189 bits (482), Expect = 4e-49






   
Genomic Context:






   
Comment:


















    ReferenceID:3189




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20336




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33319




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1593_cdd.html


    		




 
URL:

















    ReferenceID:35592




  
Author/Investigator(s):
        Alloing G, Trombe MC, Claverys JP.





  
Title:
        The ami locus of the gram-positive bacterium Streptococcus pneumoniae is similar to binding protein-dependent transport operons of gram-negative bacteria.




  
PubMed:
        2352474


MedLine:
        




    
Source:
Mol Microbiol. 1990 Apr;4(4):633-44.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2352474









           
Homologs By Global Alignment 

           

       



     
Gene ID:1593
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2818
                           PA5538_tr
                           translation of PA5538
                           397
                           99.74
                           99.99
                           0
                           1989.0
     

     
     
88041
                           gb|AAG08923.1|AE004966_5
                           (AE004966) N-acetylmuramoyl-L-alanine amidase [Pseudomonas aeruginosa PAO1]
                           397
                           99.74
                           99.99
                           0
                           1989.0
     

     
     
88042
                           gb|AAO58947.1|
                           (AE016876) N-acetylmuramoyl-L-alanine amidase, family 3 [Pseudomonas syringae pv. tomato str. DC3000]
                           411
                           58.39
                           72.99
                           18
                           1128.0
     

     
     
88048
                           gb|AAM41054.1|
                           (AE012278) N-acetylmuramoyl-L-alanine amidase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           406
                           43.59
                           57.38
                           29
                           739.0
     

     
     
88052
                           gb|AAM36644.1|
                           (AE011810) N-acetylmuramoyl-L-alanine amidase [Xanthomonas axonopodis pv. citri str. 306]
                           411
                           43.06
                           56.69
                           41
                           737.0
     

     






      

          
GC ORFID: 44595How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5538Glimmer Score: 

          
Start: 5768496Stop: 5767303

          
Length: 1194


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5538 translation of PA5538Homolog Bit Score 770.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4167 (ORF: BLASTX 440.0), t_PA5538 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGCGCCGTCGCCTCCTGCAAAGCCTGCTCGCCGGCCTGGCCCTCCAGCCGTTCCTGGCCGCCAGCGCC 
AATGTACGAATCCGCCAGGCCCGCGCCTGGAATTCCTCGGAAAGCTGGCGCCTGGTGCTGGAACTGGACGGT 
CCGCCGCGTTATCGAACCTTTTCCCTGCAGGCCCCCGAGCGGTTGATCCTCGACCTGCCCGACGCGCAATTG 
CTCGCAACGCTCTCCGAGCTGCCGCTGGACGGCCCCGTGCGCGCCATCCGCAGCGGCCAACTGGGTGGCGGC 
GGGACGCGCATCGTCCTCGACCTGCGCCAGGCGGTCAGGCCGCGCAGCTTCGCCCTGGAACCGCAGGCAGGA 
AAGGGCCATCGCCTGGTCCTGGACCTTTATCCACAGGCGCTGGCGGCTCCCCCCGAGCCGATGCTGGCGCTG 
GCCCGCCCGCTACCCTCGACGGGCCGCGACATCCTCGTGGTGGTCGATGCCGGCCATGGCGGCAAGGACCCC 
GGCGCGGTCGGTAGCAAGGGCGAGCGCGAAAAGGACGTGGCGCTGGCCATCGCCCGCCTGCTGGCGCGGCGG 
ATCGACCGCGAGAAAGGCTTCAAGGCGCGCCTGGTGCGCAACGGCGACGTGTTCGTCCCGCTGCGCAAGCGG 
CCGGAAGTGGCTCGGCGCTACAACGCCGACATGTTCGTCTCGGTGCACGCCGACGCGGCGCCGCGACTGACC 
GCCTCCGGCGCATCGGTATATGCCCTCTCCGAGCATGGCGCCAGCTCGACCATGGCGCGCTGGATGGCCGAC 
CGCGAGAACGGCGCCGACCTGATCGGCAACAGCGGCAACCTGCCGCTACAGGGCCGCGACAAGGTACTCGCC 
GGGGTGATCCTCGACATGGCCCTGAGTTCGACCATCGCCAGCAGCCTGGACCTCGGCCACAGCGTTCTCGGC 
CAGTTGTCGGACGTCACCGCGCTGCACCAGAAGCGTGTCGAGCAGGCCGGCTTCGCCGTGCTCAAGTCGCCG 
GACATCCCTTCGATCCTGGTGGAGACCGGCTTCATCTCGAATCGCGGCGATTGTCGCCGGTTGATGGACGAA 
CGCCATCGCCAGCGCCTGGCCGAGGCGATCTTCGCTGGCGTGCAGGGCTACTTCCGGCAACGCCCTCCCGCC 
GGCAGCTACCTGGCGGCGCTCAGCGCGCAGCCTCCCGGCTAG