ViewGene
Locus Name:
GeneID:
Location:

GeneID:1578
TrivialName: PA14_62960
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_62960
PAO1 Ortholog Locus:PA4760
Sequence Length:1134
Protein Length:377
Start: 5619011
Stop: 5617878
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08146.1|AE004889_9 (AE004889) DnaJ protein [Pseudomonas aeruginosa PAO1]
Identities = 377/377 (100%), Positives = 377/377 (100%)
Sequence:
ATGGCGAAACGAGACTTTTACGAGGTTCTGGGGGTTGAGCGTGGCGCCAGCGAGGCGGACCTGAAGAAGGCC
TACCGTCGCCTGGCGATGAAATACCACCCGGACCGCAATCCTGGCGACAAGGAAGCCGAGGACAAGTTCAAG
GAGGCCAACGAGGCCTACGAGGTCCTCTCCGATGCCAGCAAGCGGGCGGCCTACGACCAGTACGGCCATGCC
GGCGTCGATCCGAACATGGGTGGGGGCGCTGGCGCCGGTTTCGGCGGGGCGAGCTTCTCCGACATCTTCGGC
GATGTGTTCAGCGATTTCTTCGGTGGCGGCGGCGCCCGCGGTGGCTCGCGCGGCGGCGCGCAGCGCGGCGCG
GACCTGCGCTACACCCTGGATCTCGACCTGGAAGAGGCGGTACGCGGCACCACCGTCACCATCCGCGTGCCG
ACCCTGGTCGGTTGCAAGACTTGCAACGGCAGCGGCGCCAAGCCCGGCACCACACCTGTCACCTGCACCACT
TGCGGCGGTATCGGCCAGGTGCGCATGCAGCAGGGCTTCTTCTCGGTCCAGCAGACCTGCCCGCGCTGCCAC
GGCACCGGCAAGATGATTTCCGATCCCTGTGGCTCCTGCCATGGCCAGGGGCGCGTGGAAGAGCAGAAGACC
CTGTCGGTGAAGGTGCCGGCCGGCGTCGACACCGGCGACCGCATCCGCCTCACCGGCGAAGGCGAGGCCGGC
AGCATGGGTGGTCCGGCGGGCGACCTGTACGTGGTGGTCAATGTGCGCGAGCACCCGATCTTCCAGCGTGAC
GGCAAGCATCTGTACTGCGAAGTGCCGATCAGTTTCGCCGACGCTGCGCTGGGCGGCGAGCTGGAAGTGCCG
ACCCTGGATGGCCGGGTCAAGCTGAAGATTCCGGAAAGCACCCAGACCGGCAAGCTGTTCCGCCTGCGCGGC
AAGGGCGTGGCCCCGGTGCGCGGCGGCGGCGCCGGCGACCTGATGTGCAAGGTGGTGGTGGAAACCCCGGTG
AACCTGGACAAGCGCCAGCGCGAGCTGCTCGAGGAGTTCCGCAAGTCGTTGCAGAGCGACACCTCGCATTCG
CCCAAGGCCAGTGGCTGGTTCGAGGGCATGAAGCGTTTCTTCGACGACCTATAA
Translation:
MAKRDFYEVLGVERGASEADLKKAYRRLAMKYHPDRNPGDKEAEDKFKEANEAYEVLSDASKRAAYDQYGHA
GVDPNMGGGAGAGFGGASFSDIFGDVFSDFFGGGGARGGSRGGAQRGADLRYTLDLDLEEAVRGTTVTIRVP
TLVGCKTCNGSGAKPGTTPVTCTTCGGIGQVRMQQGFFSVQQTCPRCHGTGKMISDPCGSCHGQGRVEEQKT
LSVKVPAGVDTGDRIRLTGEGEAGSMGGPAGDLYVVVNVREHPIFQRDGKHLYCEVPISFADAALGGELEVP
TLDGRVKLKIPESTQTGKLFRLRGKGVAPVRGGGAGDLMCKVVVETPVNLDKRQRELLEEFRKSLQSDTSHS
PKASGWFEGMKRFFDDL*
AnnotationID:1587GeneID:1578
AnnotatorUID: diggins
Modification Date/Time: 2005-03-09 09:09:37
Gene Name:dnaJ
Confidence Code:2
GeneProduct:chaperone protein DnaJ
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(9) Chaperones & heat shock proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0484,pfam01556
Secondary Functional Category(ies):DNA replication, recombination and repair
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAQ22347.1| (AY344804) heat shock protein [Pseudomonas stutzeri A15]
          Length = 376

 Score =  695 bits (1794), Expect = 0.0
 Identities = 340/378 (89%), Positives = 357/378 (94%), Gaps = 3/378 (0%)

>gb|AAN70298.1|AE016670_8 (AE016791) dnaJ protein [Pseudomonas putida KT2440]
          Length = 375

 Score =  676 bits (1743), Expect = 0.0
 Identities = 325/377 (86%), Positives = 354/377 (93%), Gaps = 2/377 (0%)

>gb|AAN54197.1|AE015557_3 (AE015557) chaperone protein DnaJ [Shewanella oneidensis MR-1]
          Length = 378

 Score =  544 bits (1402), Expect = e-154
 Identities = 261/378 (69%), Positives = 305/378 (80%), Gaps = 3/378 (0%)






 
Structural Features:
COG0484, DnaJ, DnaJ-class molecular chaperone with C-terminal Zn finger domain 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 371 residues, 100.0% aligned
Score =  520 bits (1342), Expect = 9e-149
Identities = 246/378 (65%), Positives = 283/378 (74%), Gaps = 9/378 (2%)

pfam01556, DnaJ_C, DnaJ C terminal region.
CD-Length = 122 residues, 100.0% aligned
Score =  184 bits (470), Expect = 1e-47
Identities = 82/122 (67%), Positives = 94/122 (77%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3159




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20306




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37670




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1578_cdd.html


    		




 
URL:

















    ReferenceID:37717




  
Author/Investigator(s):
        Bardwell JC, Tilly K, Craig E, King J, Zylicz M, Georgopoulos C.





  
Title:
        The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein.




  
PubMed:
        3003085


MedLine:
        




    
Source:
J Biol Chem. 1986 Feb 5;261(4):1782-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=86111850&dopt=Abstract









           
Homologs By Global Alignment 

           

       



     
Gene ID:1578
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2802
                           PA4760_tr
                           translation of PA4760
                           377
                           99.99
                           99.99
                           0
                           1999.0
     

     
     
87704
                           gb|AAG08146.1|AE004889_9
                           (AE004889) DnaJ protein [Pseudomonas aeruginosa PAO1]
                           377
                           99.99
                           99.99
                           0
                           1999.0
     

     
     
87705
                           gb|AAQ22347.1|
                           (AY344804) heat shock protein [Pseudomonas stutzeri A15]
                           378
                           89.94
                           94.44
                           3
                           1798.0
     

     
     
87706
                           gb|AAN70298.1|AE016670_8
                           (AE016791) dnaJ protein [Pseudomonas putida KT2440]
                           377
                           86.20
                           93.89
                           2
                           1747.0
     

     
     
87707
                           gb|AAO57952.1|
                           (AE016872) dnaJ protein [Pseudomonas syringae pv. tomato str. DC3000]
                           380
                           85.52
                           91.57
                           3
                           1730.0
     

     






      

          
GC ORFID: 44580How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4760Glimmer Score: 

          
Start: 4882691Stop: 4881558

          
Length: 1134


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4760 translation of PA4760Homolog Bit Score 774.0

          
Other Homologs:  psyr_15may02_Scaffold4_revised_gene5478 (ORF: BLASTX 665.0), t_PA4760 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGAAACGAGACTTTTACGAGGTTCTGGGGGTTGAGCGTGGCGCCAGCGAGGCGGACCTGAAGAAGGCC 
TACCGTCGCCTGGCGATGAAATACCACCCGGACCGCAATCCTGGCGACAAGGAAGCCGAGGACAAGTTCAAG 
GAGGCCAACGAGGCCTACGAGGTCCTCTCCGATGCCAGCAAGCGGGCGGCCTACGACCAGTACGGCCATGCC 
GGCGTCGATCCGAACATGGGTGGGGGCGCTGGCGCCGGTTTCGGCGGGGCGAGCTTCTCCGACATCTTCGGC 
GATGTGTTCAGCGATTTCTTCGGTGGCGGCGGCGCCCGCGGTGGCTCGCGCGGCGGCGCGCAGCGCGGCGCG 
GACCTGCGCTACACCCTGGATCTCGACCTGGAAGAGGCGGTACGCGGCACCACCGTCACCATCCGCGTGCCG 
ACCCTGGTCGGTTGCAAGACTTGCAACGGCAGCGGCGCCAAGCCCGGCACCACACCTGTCACCTGCACCACT 
TGCGGCGGTATCGGCCAGGTGCGCATGCAGCAGGGCTTCTTCTCGGTCCAGCAGACCTGCCCGCGCTGCCAC 
GGCACCGGCAAGATGATTTCCGATCCCTGTGGCTCCTGCCATGGCCAGGGGCGCGTGGAAGAGCAGAAGACC 
CTGTCGGTGAAGGTGCCGGCCGGCGTCGACACCGGCGACCGCATCCGCCTCACCGGCGAAGGCGAGGCCGGC 
AGCATGGGTGGTCCGGCGGGCGACCTGTACGTGGTGGTCAATGTGCGCGAGCACCCGATCTTCCAGCGTGAC 
GGCAAGCATCTGTACTGCGAAGTGCCGATCAGTTTCGCCGACGCTGCGCTGGGCGGCGAGCTGGAAGTGCCG 
ACCCTGGATGGCCGGGTCAAGCTGAAGATTCCGGAAAGCACCCAGACCGGCAAGCTGTTCCGCCTGCGCGGC 
AAGGGCGTGGCCCCGGTGCGCGGCGGCGGCGCCGGCGACCTGATGTGCAAGGTGGTGGTGGAAACCCCGGTG 
AACCTGGACAAGCGCCAGCGCGAGCTGCTCGAGGAGTTCCGCAAGTCGTTGCAGAGCGACACCTCGCATTCG 
CCCAAGGCCAGTGGCTGGTTCGAGGGCATGAAGCGTTTCTTCGACGACCTATAA