ViewGene
Locus Name:
GeneID:
Location:

GeneID:1574
TrivialName: PA14_05080
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05080
PAO1 Ortholog Locus:PA0390
Sequence Length:1140
Protein Length:379
Start: 446505
Stop: 445366
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03779.1|AE004476_9 (AE004476) homoserine O-acetyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 379/379 (100%), Positives = 379/379 (100%)
Sequence:
ATGCCCACAGTCTTCCCCGACGACTCCGTCGGTCTGGTCTCCCCCCAGACGCTGCACTTCAACGAACCGCTC
GAGCTGACCAGCGGCAAGTCCCTGGCCGAGTACGACCTGGTGATCGAAACCTACGGCGAGCTGAATGCCACG
CAGAGCAACGCGGTGCTGATCTGCCACGCCCTCTCCGGCCACCACCACGCCGCCGGCTACCACAGCGTCGAC
GAGCGCAAGCCGGGCTGGTGGGACAGCTGCATCGGTCCGGGCAAGCCGATCGACACCCGCAAGTTCTTCGTC
GTCGCCCTCAACAACCTCGGCGGTTGCAACGGATCCAGCGGCCCCGCCAGCATCAATCCGGCGACCGGCAAG
GTCTACGGCGCGGACTTCCCGATGGTTACGGTGGAAGACTGGGTGCATAGCCAGGCGCGCCTGGCAGACCGC
CTCGGCATCCGCCAGTGGGCCGCGGTGGTCGGCGGCAGCCTCGGCGGCATGCAGGCGCTGCAATGGACCATC
AGCTATCCCGAGCGCGTCCGTCACTGCCTGTGCATCGCCAGCGCGCCGAAGCTGTCGGCGCAGAACATCGCC
TTCAACGAAGTCGCCCGGCAGGCGATTCTTTCCGACCCTGAGTTCCTCGGCGGCTACTTCCAGGAACAGGGC
GTGATTCCCAAGCGCGGTCTCAAGCTGGCGCGGATGGTCGGCCATATCACCTACCTGTCCGACGACGCCATG
GGCGCCAAGTTCGGCCGCGTACTGAAGACCGAGAAGCTCAACTACGACCTGCACAGCGTCGAGTTCCAGGTC
GAGAGCTACCTGCGCTACCAGGGCGAGGAGTTCTCCACCCGCTTCGACGCCAATACCTACCTGCTGATGACC
AAGGCGCTGGACTACTTCGACCCCGCCGCCGCCCACGGCGACGACCTGGTGCGCACCCTGGAGGGCGTCGAG
GCGGACTTCTGCCTGATGTCCTTCACCACCGACTGGCGTTTCTCGCCGGCCCGCTCGCGGGAAATCGTCGAC
GCCCTGATCGCGGCGAAAAAGAACGTCAGCTACCTGGAGATCGACGCCCCGCAAGGCCACGACGCCTTCCTC
ATGCCGATCCCCCGGTACCTGCAAGCCTTCAGCGGTTACATGAACCGCATCAGCGTGTGA
Translation:
MPTVFPDDSVGLVSPQTLHFNEPLELTSGKSLAEYDLVIETYGELNATQSNAVLICHALSGHHHAAGYHSVD
ERKPGWWDSCIGPGKPIDTRKFFVVALNNLGGCNGSSGPASINPATGKVYGADFPMVTVEDWVHSQARLADR
LGIRQWAAVVGGSLGGMQALQWTISYPERVRHCLCIASAPKLSAQNIAFNEVARQAILSDPEFLGGYFQEQG
VIPKRGLKLARMVGHITYLSDDAMGAKFGRVLKTEKLNYDLHSVEFQVESYLRYQGEEFSTRFDANTYLLMT
KALDYFDPAAAHGDDLVRTLEGVEADFCLMSFTTDWRFSPARSREIVDALIAAKKNVSYLEIDAPQGHDAFL
MPIPRYLQAFSGYMNRISV*
AnnotationID:1583GeneID:1574
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 06:06:36
Gene Name:metX
Confidence Code:2
GeneProduct:homoserine O-acetyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2021
Secondary Functional Category(ies):
EC Number:2.3.1.31
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70662.1|AE016710_7 (AE016793) homoserine O-acetyltransferase [Pseudomonas putida
           KT2440]
          Length = 379

 Score =  654 bits (1686), Expect = 0.0
 Identities = 311/379 (82%), Positives = 342/379 (90%)

>gb|AAO58477.1| (AE016874) homoserine O-acetyltransferase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 379

 Score =  647 bits (1670), Expect = 0.0
 Identities = 311/379 (82%), Positives = 341/379 (89%)

>gb|AAF41346.1| (AE002445) homoserine O-acetyltransferase [Neisseria meningitidis
           MC58]
          Length = 379

 Score =  514 bits (1325), Expect = e-145
 Identities = 245/369 (66%), Positives = 298/369 (80%), Gaps = 1/369 (0%)






 
Structural Features:
COG2021, MET2, Homoserine acetyltransferase 
[Amino acid transport and metabolism]
CD-Length = 368 residues, 100.0% aligned
Score =  481 bits (1240), Expect = 5e-137
Identities = 191/378 (50%), Positives = 247/378 (65%), Gaps = 11/378 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3151




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20298




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33727




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1574_cdd.html


    		




 
URL:

















    ReferenceID:35385




  
Author/Investigator(s):
        Andersen GL, Beattie GA, Lindow SE.





  
Title:
        Molecular characterization and sequence of a methionine biosynthetic locus from Pseudomonas syringae.




  
PubMed:
        9721288


MedLine:
        




    
Source:
J Bacteriol. 1998 Sep;180(17):4497-507.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9721288

















    ReferenceID:35386




  
Author/Investigator(s):
        Bourhy P, Martel A, Margarita D, Saint Girons I, Belfaiza J.





  
Title:
        Homoserine O-acetyltransferase, involved in the Leptospira meyeri methionine biosynthetic pathway, is not feedback inhibited.




  
PubMed:
        9209059


MedLine:
        




    
Source:
J Bacteriol. 1997 Jul;179(13):4396-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9209059









           
Homologs By Global Alignment 

           

       



     
Gene ID:1574
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2798
                           PA0390_tr
                           translation of PA0390
                           379
                           99.99
                           99.99
                           0
                           2001.0
     

     
     
87620
                           gb|AAG03779.1|AE004476_9
                           (AE004476) homoserine O-acetyltransferase [Pseudomonas aeruginosa PAO1]
                           379
                           99.99
                           99.99
                           0
                           2001.0
     

     
     
87621
                           gb|AAN70662.1|AE016710_7
                           (AE016793) homoserine O-acetyltransferase [Pseudomonas putida KT2440]
                           379
                           82.05
                           90.23
                           0
                           1686.0
     

     
     
87622
                           gb|AAO58477.1|
                           (AE016874) homoserine O-acetyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           379
                           82.05
                           89.97
                           0
                           1670.0
     

     
     
87623
                           gb|AAQ58462.1|
                           (AE016912) probable homoserine O-acetyltransferase [Chromobacterium violaceum ATCC 12472]
                           385
                           64.41
                           78.44
                           11
                           1323.0
     

     






      

          
GC ORFID: 44576How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0390Glimmer Score: 

          
Start: 6247847Stop: 6246708

          
Length: 1140


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0390 translation of PA0390Homolog Bit Score 775.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3721 (ORF: BLASTX 650.0), t_PA0390 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCACAGTCTTCCCCGACGACTCCGTCGGTCTGGTCTCCCCCCAGACGCTGCACTTCAACGAACCGCTC 
GAGCTGACCAGCGGCAAGTCCCTGGCCGAGTACGACCTGGTGATCGAAACCTACGGCGAGCTGAATGCCACG 
CAGAGCAACGCGGTGCTGATCTGCCACGCCCTCTCCGGCCACCACCACGCCGCCGGCTACCACAGCGTCGAC 
GAGCGCAAGCCGGGCTGGTGGGACAGCTGCATCGGTCCGGGCAAGCCGATCGACACCCGCAAGTTCTTCGTC 
GTCGCCCTCAACAACCTCGGCGGTTGCAACGGATCCAGCGGCCCCGCCAGCATCAATCCGGCGACCGGCAAG 
GTCTACGGCGCGGACTTCCCGATGGTTACGGTGGAAGACTGGGTGCATAGCCAGGCGCGCCTGGCAGACCGC 
CTCGGCATCCGCCAGTGGGCCGCGGTGGTCGGCGGCAGCCTCGGCGGCATGCAGGCGCTGCAATGGACCATC 
AGCTATCCCGAGCGCGTCCGTCACTGCCTGTGCATCGCCAGCGCGCCGAAGCTGTCGGCGCAGAACATCGCC 
TTCAACGAAGTCGCCCGGCAGGCGATTCTTTCCGACCCTGAGTTCCTCGGCGGCTACTTCCAGGAACAGGGC 
GTGATTCCCAAGCGCGGTCTCAAGCTGGCGCGGATGGTCGGCCATATCACCTACCTGTCCGACGACGCCATG 
GGCGCCAAGTTCGGCCGCGTACTGAAGACCGAGAAGCTCAACTACGACCTGCACAGCGTCGAGTTCCAGGTC 
GAGAGCTACCTGCGCTACCAGGGCGAGGAGTTCTCCACCCGCTTCGACGCCAATACCTACCTGCTGATGACC 
AAGGCGCTGGACTACTTCGACCCCGCCGCCGCCCACGGCGACGACCTGGTGCGCACCCTGGAGGGCGTCGAG 
GCGGACTTCTGCCTGATGTCCTTCACCACCGACTGGCGTTTCTCGCCGGCCCGCTCGCGGGAAATCGTCGAC 
GCCCTGATCGCGGCGAAAAAGAACGTCAGCTACCTGGAGATCGACGCCCCGCAAGGCCACGACGCCTTCCTC 
ATGCCGATCCCCCGGTACCTGCAAGCCTTCAGCGGTTACATGAACCGCATCAGCGTGTGA