ViewGene
Locus Name:
GeneID:
Location:

GeneID:1571
TrivialName: PA14_17880
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17880
PAO1 Ortholog Locus:PA3589
Sequence Length:1206
Protein Length:401
Start: 1534832
Stop: 1536037
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06977.1|AE004779_9 (AE004779) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
Identities = 401/401 (100%), Positives = 401/401 (100%)
Sequence:
ATGCTCGATGCCTATATCTACGCCGGCCTGCGTACGCCTTTCGGCCGGCATGCCGGTGCACTCTCGACGGTG
CGTCCGGACGACCTGGCCGGCCTGCTGCTGGCGCGTCTCGCGGAAACCTCCGGGTTCGCCGTCGACGACCTG
GAGGATGTGATCCTCGGTTGCACCAACCAGGCCGGCGAAGACAGCCGCAACCTGGCGCGCAACGCGCTGCTC
GCAGCCGGCCTGCCGGCGCGGCTGCCTGGGCAGACGGTCAACCGCTTGTGTGCCAGCGGACTGTCGGCGGTG
ATCGACGCGGCGCGCGCGATCAGTTGCGGCGAGGGCCGGCTGTACCTGGCCGGCGGCGCCGAAAGCATGTCC
CGGGCGCCGTTCGTCATGGGCAAGGCGGAGAGCGCCTTCAGCCGCACGCTGGAGGTCTTCGACAGCACCATC
GGCGCGCGCTTCGCCAACCCCAGGCTGGTCGAGCGCTATGGCAACGACAGCATGCCGGAGACCGGCGACAAC
GTGGCCCGCGCCTTCGGCATCGCCCGCGAGGATGCCGACCGTTTCGCCGCTTCTTCCCAGGCGCGCTACCAG
GCTGCGCTGGAGGAGGGCTTTTTCCTCGGCGAAATCCTTCCGGTGGAGGTGCGCGCCGGACGCAAGGGCGAG
ACGCGGCTGGTGGAGCGCGACGAGCATCCGCGGCCGCAGGCCGACCTGGCGGCCCTGGCGCGCTTGCCGGCG
TTGTTCGCCGGCGGGGTAGTGACCGCCGGCAACGCGTCGGGGATCAACGACGGGGCGGCGGTAGTGCTGCTG
GGCGACCGCGCGATCGGCGAGCGCGAGGGCATCCGGCCGCTGGCGCGGATCCTCGCTAGCGCCAGCGTCGGC
GTGGAGCCCCGGTTGATGGGCATCGGCCCGCAGCAGGCGATCCTCCGCGCGCTGCAACGCGCCGGCATCGAC
CTGGACGAGGTCGGCCTGATCGAGATCAACGAAGCCTTCGCGCCGCAGGTCCTGGCCTGCCTGAAGCTGCTC
GGCCTGGACTACGAGGACCCGCGGGTCAATCCCCATGGCGGCGCCATCGCCCTCGGCCATCCGCTCGGCGCC
TCCGGTGCGCGCCTGGTGCTCACCGCCGCCCGCGGGCTGCAACGCATCGAGCGGCGCTACGCGGTGGTCAGC
CTGTGCGTCGGGCTCGGCCAGGGCGTGGCGATGGTGATCGAGCGCTGCCGATGA
Translation:
MLDAYIYAGLRTPFGRHAGALSTVRPDDLAGLLLARLAETSGFAVDDLEDVILGCTNQAGEDSRNLARNALL
AAGLPARLPGQTVNRLCASGLSAVIDAARAISCGEGRLYLAGGAESMSRAPFVMGKAESAFSRTLEVFDSTI
GARFANPRLVERYGNDSMPETGDNVARAFGIAREDADRFAASSQARYQAALEEGFFLGEILPVEVRAGRKGE
TRLVERDEHPRPQADLAALARLPALFAGGVVTAGNASGINDGAAVVLLGDRAIGEREGIRPLARILASASVG
VEPRLMGIGPQQAILRALQRAGIDLDEVGLIEINEAFAPQVLACLKLLGLDYEDPRVNPHGGAIALGHPLGA
SGARLVLTAARGLQRIERRYAVVSLCVGLGQGVAMVIERCR*
AnnotationID:1580GeneID:1571
AnnotatorUID: diggins
Modification Date/Time: 2005-07-04 07:07:19
Gene Name:
Confidence Code:3
GeneProduct:acetyl-CoA acetyltransferase (thiolase)
Cell Localization:()
Synonyms:paaJ
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:Beta-ketoadipyl CoA thiolase
Functional Category Confidence Code:5
COGs:KOG1390,COG0183,KOG1389
Secondary Functional Category(ies):carbon compound metabolism
EC Number:2.3.1.-
Status:ACTIVE
Pathway:
Phenylacetic acid aerobic catabolism.
Homology:
>dbj|BAB61748.1| (AB026669) acetyl-CoA acyltransferase-like protein [Acinetobacter
           sp. NCIMB9871]
          Length = 400

 Score =  525 bits (1352), Expect = e-148
 Identities = 261/399 (65%), Positives = 325/399 (81%)

>gb|AAC24332.1| (AF029714) PaaE [Pseudomonas putida]
          Length = 497

 Score =  413 bits (1062), Expect = e-115
 Identities = 229/404 (56%), Positives = 274/404 (67%), Gaps = 9/404 (2%)

>gb|AAN68887.1|AE016520_7 (AE016786) beta-ketoadipyl CoA thiolase PhaD [Pseudomonas putida
           KT2440]
          Length = 406

 Score =  412 bits (1060), Expect = e-114
 Identities = 227/402 (56%), Positives = 273/402 (67%), Gaps = 9/402 (2%)
Structural Features:
KOG1390, Acetyl-CoA acetyltransferase [Lipid transport and metabolism]
CD-Length = 396 residues,  98.2% aligned
Score =  292 bits (750), Expect = 7e-80
Identities = 154/399 (38%), Positives = 227/399 (56%), Gaps = 12/399 (3%)

COG0183, PaaJ, Acetyl-CoA acetyltransferase [Lipid metabolism]
CD-Length = 392 residues,  99.7% aligned
Score =  273 bits (700), Expect = 4e-74
Identities = 170/412 (41%), Positives = 217/412 (52%), Gaps = 34/412 (8%)

KOG1389, 3-oxoacyl CoA thiolase [Lipid transport and metabolism]
CD-Length = 435 residues,  89.0% aligned
Score =  267 bits (683), Expect = 5e-72
Identities = 157/408 (38%), Positives = 212/408 (51%), Gaps = 32/408 (7%)
Genomic Context:






   
Comment:
other possible gene name: pcaF


















    ReferenceID:3145




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20292




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31954




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1571_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:1571
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2792
                           PA3589_tr
                           translation of PA3589
                           401
                           99.99
                           99.99
                           0
                           2002.0
     

     
     
87393
                           gb|AAG06977.1|AE004779_9
                           (AE004779) probable acyl-CoA thiolase [Pseudomonas aeruginosa PAO1]
                           401
                           99.99
                           99.99
                           0
                           2002.0
     

     
     
87394
                           dbj|BAB61748.1|
                           (AB026669) acetyl-CoA acyltransferase-like protein [Acinetobacter sp. NCIMB9871]
                           401
                           65.08
                           81.04
                           1
                           1351.0
     

     
     
87395
                           gb|AAQ61951.1|
                           (AE016925) Beta-ketoadipyl CoA thiolase [Chromobacterium violaceum ATCC 12472]
                           407
                           56.51
                           71.00
                           13
                           1072.0
     

     
     
87398
                           emb|CAE45106.1|
                           (AJ579894) putative ketothiolase [Pseudomonas sp. Y2]
                           405
                           56.04
                           68.64
                           6
                           1064.0
     

     






      

          
GC ORFID: 44573How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3589Glimmer Score: 

          
Start: 3419104Stop: 3417899

          
Length: 1206


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3589 translation of PA3589Homolog Bit Score 775.0

          
Other Homologs:  t_PA0228 (ORF: BLASTX 406.0), psyr_15may02_Scaffold5_revised_gene6193 (ORF: BLASTX 390.0), t_PA2001 (ORF: BLASTX 279.0), psyr_15may02_Scaffold15_revised_gene2524 (ORF: BLASTX 264.0), t_PA2940 (ORF: BLASTX 250.0), t_PA3589 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCGATGCCTATATCTACGCCGGCCTGCGTACGCCTTTCGGCCGGCATGCCGGTGCACTCTCGACGGTG 
CGTCCGGACGACCTGGCCGGCCTGCTGCTGGCGCGTCTCGCGGAAACCTCCGGGTTCGCCGTCGACGACCTG 
GAGGATGTGATCCTCGGTTGCACCAACCAGGCCGGCGAAGACAGCCGCAACCTGGCGCGCAACGCGCTGCTC 
GCAGCCGGCCTGCCGGCGCGGCTGCCTGGGCAGACGGTCAACCGCTTGTGTGCCAGCGGACTGTCGGCGGTG 
ATCGACGCGGCGCGCGCGATCAGTTGCGGCGAGGGCCGGCTGTACCTGGCCGGCGGCGCCGAAAGCATGTCC 
CGGGCGCCGTTCGTCATGGGCAAGGCGGAGAGCGCCTTCAGCCGCACGCTGGAGGTCTTCGACAGCACCATC 
GGCGCGCGCTTCGCCAACCCCAGGCTGGTCGAGCGCTATGGCAACGACAGCATGCCGGAGACCGGCGACAAC 
GTGGCCCGCGCCTTCGGCATCGCCCGCGAGGATGCCGACCGTTTCGCCGCTTCTTCCCAGGCGCGCTACCAG 
GCTGCGCTGGAGGAGGGCTTTTTCCTCGGCGAAATCCTTCCGGTGGAGGTGCGCGCCGGACGCAAGGGCGAG 
ACGCGGCTGGTGGAGCGCGACGAGCATCCGCGGCCGCAGGCCGACCTGGCGGCCCTGGCGCGCTTGCCGGCG 
TTGTTCGCCGGCGGGGTAGTGACCGCCGGCAACGCGTCGGGGATCAACGACGGGGCGGCGGTAGTGCTGCTG 
GGCGACCGCGCGATCGGCGAGCGCGAGGGCATCCGGCCGCTGGCGCGGATCCTCGCTAGCGCCAGCGTCGGC 
GTGGAGCCCCGGTTGATGGGCATCGGCCCGCAGCAGGCGATCCTCCGCGCGCTGCAACGCGCCGGCATCGAC 
CTGGACGAGGTCGGCCTGATCGAGATCAACGAAGCCTTCGCGCCGCAGGTCCTGGCCTGCCTGAAGCTGCTC 
GGCCTGGACTACGAGGACCCGCGGGTCAATCCCCATGGCGGCGCCATCGCCCTCGGCCATCCGCTCGGCGCC 
TCCGGTGCGCGCCTGGTGCTCACCGCCGCCCGCGGGCTGCAACGCATCGAGCGGCGCTACGCGGTGGTCAGC 
CTGTGCGTCGGGCTCGGCCAGGGCGTGGCGATGGTGATCGAGCGCTGCCGATGA