ViewGene
Locus Name:
GeneID:
Location:

GeneID:157
TrivialName: PA14_16860
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16860
PAO1 Ortholog Locus:PA3673
Sequence Length:2505
Protein Length:834
Start: 1445735
Stop: 1443231
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07061.1|AE004787_1 (AE004787) glycerol-3-phosphate acyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 832/834 (99%), Positives = 833/834 (99%)
Sequence:
ATGCCCCGCTACCCTTTCCGCCGCTTTGGTTTTGGCGCCCTGCGCCGCCTGCTGTACCTCTGGGTACGCTCG
GAAACCATCAACCAATCGGCCTTCACCCTGAAGATCGACCGCAGCAAGCCGGTGCTTTACGTGCTGCAGCAG
CCTTCGGTCAGCGATCTCGCGGTGGTGGATACCGAATGCCGCAAGGCCGGGCTGCCGCGTCCGGTGATGCCG
GTAGCGGTAGGCGACGCCATCGAGCCGGCGGCGTTCTTCTACCTGACGCCCGAACCCGACTGGCTGGGCCGC
CAGGACAAGCGCGGCGCCTCGCCGACCCTGGTGCGCATGCTCGCCGCGGTCGGCCAGAACGGTCTCGACGAC
GCGCAGATCATTCCGGTCAGCGTGTTCTGGGGACAATCGCCGGACAGCGAATCGAGCCCCTGGAAGCTGCTG
TTCGCCGACAACTGGGCGGTCACCGGGCGCCTGCGCAAGCTGGCGCGCATCCTGATTCTCGGACGCAAGACC
CGCGTGCAGTTCTCCGCGCCGATCCACCTGCGCGAGCTGGTGGAACAGGGCAAGGGCCACGAACGCACGCTG
CGCATGGTCAACCGTATCCTCCGGGTGCACTTCCGCAACCTGAAGACCGCGGTAATCGGTCCCGACCTGTCG
CACCGCCGCAACCTGGTCAAGGGCCTGCTGCGCGCGCCGCTGGTACGCCAGGCGATCAACGAGGAATGCGAG
AGCGAACGGATATCCCAGGAGAAGGCCGAAGGCATCGCGCTGCGCTACGCCAATGAAATCGCCTCGGACTTC
TCCTATCCGGTGATCCGCTTCCTCGAAGTCATTCTCAGCTGGTTCTGGAACAAGCTGTACGAAGGGGTCAAG
GTCAACCACATCGAGCGGGTGCAGGACGTGGCCCAGGGCAACGAGATCGTCTACGTGCCCTGCCACCGCAGC
CACATCGACTACCTGCTGCTCTCCTACCTGCTGTTCCGCAACGGCCTGACCCCGCCGCACATCGCCGCCGGG
ATCAACCTGAACATGCCGGTGATCGGCTCGATCCTGCGCCGCGGCGGAGCCTTCTTCATGCGCCGCAGCTTC
AAGGGCAACCAGCTCTACACCGCGGTGTTCAACGAGTACCTGCACACCCTGTTCTCCCGCGGCTTCTCCACC
GAGTACTTCGTCGAGGGTGGCCGTTCGCGCACCGGGCGCATGCTCCACCCACGCACCGGCATGCTCGCCATC
ACCCTGCGCAGCTTCCTCCGAGACTCGCGCCGGCCGATCGTCTTCGTACCGGTCTACATCGGCTACGAACGC
GTGCTGGAAGGCCGCACCTACCTCGGCGAACTGCGCGGCGCGACCAAGAAGAAGGAGTCGATCTTCGACCTG
TTCAAGGTGGTCGGCGCCCTGAAACAGCGTTTCGGCCAGGTCTGGGTGAACTTCGGCGAGCCGATCCATCTC
GACCAGTTCCTCGACCGCCACCAGCCCGACTGGCAGGACCAGGACCTCGGCCCGGAATACCGCCCCGACTGG
CTGCCGCAGACCACCAACCTGCTGGCCAAGGACGTCGCTCGCCACCTCAACGACGCGGCGGCGATCAACCCG
GTCAACCTGGTCGCCCTGGCGCTGCTCTCCACCAGCCGCCAGGCCCTCGACGAAAGCGCCCTGGCGCGAATC
CTCGACCTCTACCTGGCGCTGCTGCGCAAGGTGCCCTACTCGCCCAGCGCGACCCTGCCGGACGGCGACGGC
CAGGCCCTGATCGAGTACGTGAAGAGCATGAACCTGCTGGCCGAGCAGAAGGACGCGCTGGGCCGCATCCTC
TATCTCGACGAACAGAACGCGGTGCTGGCGACCTACTACCGCAACAACGTCCTCCACGTCTTCGCCCTGCCG
GCGCTGATCGCCAGCTTCTTCCAGAGCAACTCGCGGATCAGCCGCGAACAGTTGCTGCGCTTCGCCCGCGCG
CTCTATCCCTACCTGCAGGCGGAACTGTTCATCCGCTGGAGCCTGGACGAACTCGACGCGGTGATCGACCAG
TGGCTGGCGGCCCTGGTGGAGCAGGGCCTGCTGCGCCAGGAGAACGACACCTTCATCCGCCCGGCGCCAAGC
TCGCGGCAATACGTGCTGCTGATCCTCCTCGCTCGTTCGGTGACCCAGACCCTGCAGCGCTTCTACATGGCC
ATCGCCCTGCTGCTCAACGCCGGGCAGAACGCCCTGACCGCCGAGGAACTGGAGAACCTCTGCACCGTGATG
GCCCAGCGCCTGTCGATCCTGCATGGCCTGAACGCCCCGGAGTTCTTCGACAAGAGCCTGTTCCGCCACTTC
ATCCAGACCCTTCTCGACCTGCGTGTGCTGCGCAAGGACGAGGCCGGCAAACTGAGCTACCACGAACTGCTC
GGCGAACTGGCCGAAGGCGCCGCCAAGCGCGTGCTGCCGGCGGAGATCCGCCTGTCGATCCGCCAGGTGGCG
CTGGAGCGTCCCGCCGAGGAAGCCGCCGCCGAAAGCAACGACGCAGCCGCCAACTGA
Translation:
MPRYPFRRFGFGALRRLLYLWVRSETINQSAFTLKIDRSKPVLYVLQQPSVSDLAVVDTECRKAGLPRPVMP
VAVGDAIEPAAFFYLTPEPDWLGRQDKRGASPTLVRMLAAVGQNGLDDAQIIPVSVFWGQSPDSESSPWKLL
FADNWAVTGRLRKLARILILGRKTRVQFSAPIHLRELVEQGKGHERTLRMVNRILRVHFRNLKTAVIGPDLS
HRRNLVKGLLRAPLVRQAINEECESERISQEKAEGIALRYANEIASDFSYPVIRFLEVILSWFWNKLYEGVK
VNHIERVQDVAQGNEIVYVPCHRSHIDYLLLSYLLFRNGLTPPHIAAGINLNMPVIGSILRRGGAFFMRRSF
KGNQLYTAVFNEYLHTLFSRGFSTEYFVEGGRSRTGRMLHPRTGMLAITLRSFLRDSRRPIVFVPVYIGYER
VLEGRTYLGELRGATKKKESIFDLFKVVGALKQRFGQVWVNFGEPIHLDQFLDRHQPDWQDQDLGPEYRPDW
LPQTTNLLAKDVARHLNDAAAINPVNLVALALLSTSRQALDESALARILDLYLALLRKVPYSPSATLPDGDG
QALIEYVKSMNLLAEQKDALGRILYLDEQNAVLATYYRNNVLHVFALPALIASFFQSNSRISREQLLRFARA
LYPYLQAELFIRWSLDELDAVIDQWLAALVEQGLLRQENDTFIRPAPSSRQYVLLILLARSVTQTLQRFYMA
IALLLNAGQNALTAEELENLCTVMAQRLSILHGLNAPEFFDKSLFRHFIQTLLDLRVLRKDEAGKLSYHELL
GELAEGAAKRVLPAEIRLSIRQVALERPAEEAAAESNDAAAN*
AnnotationID:167GeneID:157
AnnotatorUID: diggins
Modification Date/Time: 2005-06-19 08:08:38
Gene Name:plsB
Confidence Code:2
GeneProduct:glycerol-3-phosphate acyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2937
Secondary Functional Category(ies):
EC Number:2.3.1.15
Status:ACTIVE
Pathway:
Glycerolipid metabolism;
Glycerophospholipid metabolism;
De novo phospholipid biosynthesis; first step.
Homology:
>gb|AAO55040.1| (AE016861) glycerol-3-phosphate acyltransferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 833

 Score = 1317 bits (3409), Expect = 0.0
 Identities = 645/833 (77%), Positives = 737/833 (88%)

>gb|AAF83841.1|AE003940_5 (AE003940) glycerol-3-phosphate acyltransferase [Xylella fastidiosa
           9a5c]
          Length = 870

 Score =  637 bits (1643), Expect = 0.0
 Identities = 337/788 (42%), Positives = 499/788 (63%), Gaps = 10/788 (1%)

>gb|AAN83437.1|AE016770_237 (AE016770) Glycerol-3-phosphate acyltransferase [Escherichia coli
           CFT073]
          Length = 827

 Score =  571 bits (1472), Expect = e-162
 Identities = 333/830 (40%), Positives = 489/830 (58%), Gaps = 61/830 (7%)
Structural Features:
COG2937, PlsB, Glycerol-3-phosphate O-acyltransferase [Lipid metabolism]
CD-Length = 810 residues, 100.0% aligned
Score =  970 bits (2508), Expect = 0.0
Identities = 382/824 (46%), Positives = 501/824 (60%), Gaps = 18/824 (2%)
Genomic Context:






   
Comment:


















    ReferenceID:317




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17459




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31872




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:157_cdd.html


    		




 
URL:

















    ReferenceID:34238




  
Author/Investigator(s):
        Lightner VA, Bell RM, Modrich P.





  
Title:
        The DNA sequences encoding plsB and dgk loci of Escherichia coli.




  
PubMed:
        6309817


MedLine:
        




    
Source:
J Biol Chem. 1983 Sep 25;258(18):10856-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6309817

















    ReferenceID:34239




  
Author/Investigator(s):
        Lightner VA, Larson TJ, Tailleur P, Kantor GD, Raetz CR, Bell RM, Modrich P.





  
Title:
        Membrane phospholipid synthesis in Escherichia coli. Cloning of a structural gene (plsB) of the sn-glycerol-3-phosphate acyl/transferase.




  
PubMed:
        6251087


MedLine:
        




    
Source:
J Biol Chem. 1980 Oct 10;255(19):9413-20.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6251087









           
Homologs By Global Alignment 

           

       



     
Gene ID:157
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
283
                           PA3673_tr
                           translation of PA3673
                           834
                           99.76
                           99.88
                           0
                           4246.0
     

     
     
18332
                           gb|AAG07061.1|AE004787_1
                           (AE004787) glycerol-3-phosphate acyltransferase [Pseudomonas aeruginosa PAO1]
                           834
                           99.76
                           99.88
                           0
                           4246.0
     

     
     
18333
                           gb|AAO55040.1|
                           (AE016861) glycerol-3-phosphate acyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           834
                           77.33
                           88.36
                           1
                           3409.0
     

     
     
18334
                           gb|AAN67141.1|AE016343_1
                           (AE016779) glycerol-3-phosphate acyltransferase [Pseudomonas putida KT2440]
                           834
                           75.77
                           86.81
                           6
                           3320.0
     

     
     
18336
                           gb|AAM39105.1|
                           (AE012079) glycerol-3-phosphate acyltransferase [Xanthomonas axonopodis pv. citri str. 306]
                           906
                           40.61
                           58.27
                           93
                           1706.0
     

     






      

          
GC ORFID: 43159How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3673Glimmer Score: 

          
Start: 3508202Stop: 3510706

          
Length: 2505


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3673 translation of PA3673Homolog Bit Score 1640.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4810 (ORF: BLASTX 1310.0), t_PA3673 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCCGCTACCCTTTCCGCCGCTTTGGTTTTGGCGCCCTGCGCCGCCTGCTGTACCTCTGGGTACGCTCG 
GAAACCATCAACCAATCGGCCTTCACCCTGAAGATCGACCGCAGCAAGCCGGTGCTTTACGTGCTGCAGCAG 
CCTTCGGTCAGCGATCTCGCGGTGGTGGATACCGAATGCCGCAAGGCCGGGCTGCCGCGTCCGGTGATGCCG 
GTAGCGGTAGGCGACGCCATCGAGCCGGCGGCGTTCTTCTACCTGACGCCCGAACCCGACTGGCTGGGCCGC 
CAGGACAAGCGCGGCGCCTCGCCGACCCTGGTGCGCATGCTCGCCGCGGTCGGCCAGAACGGTCTCGACGAC 
GCGCAGATCATTCCGGTCAGCGTGTTCTGGGGACAATCGCCGGACAGCGAATCGAGCCCCTGGAAGCTGCTG 
TTCGCCGACAACTGGGCGGTCACCGGGCGCCTGCGCAAGCTGGCGCGCATCCTGATTCTCGGACGCAAGACC 
CGCGTGCAGTTCTCCGCGCCGATCCACCTGCGCGAGCTGGTGGAACAGGGCAAGGGCCACGAACGCACGCTG 
CGCATGGTCAACCGTATCCTCCGGGTGCACTTCCGCAACCTGAAGACCGCGGTAATCGGTCCCGACCTGTCG 
CACCGCCGCAACCTGGTCAAGGGCCTGCTGCGCGCGCCGCTGGTACGCCAGGCGATCAACGAGGAATGCGAG 
AGCGAACGGATATCCCAGGAGAAGGCCGAAGGCATCGCGCTGCGCTACGCCAATGAAATCGCCTCGGACTTC 
TCCTATCCGGTGATCCGCTTCCTCGAAGTCATTCTCAGCTGGTTCTGGAACAAGCTGTACGAAGGGGTCAAG 
GTCAACCACATCGAGCGGGTGCAGGACGTGGCCCAGGGCAACGAGATCGTCTACGTGCCCTGCCACCGCAGC 
CACATCGACTACCTGCTGCTCTCCTACCTGCTGTTCCGCAACGGCCTGACCCCGCCGCACATCGCCGCCGGG 
ATCAACCTGAACATGCCGGTGATCGGCTCGATCCTGCGCCGCGGCGGAGCCTTCTTCATGCGCCGCAGCTTC 
AAGGGCAACCAGCTCTACACCGCGGTGTTCAACGAGTACCTGCACACCCTGTTCTCCCGCGGCTTCTCCACC 
GAGTACTTCGTCGAGGGTGGCCGTTCGCGCACCGGGCGCATGCTCCACCCACGCACCGGCATGCTCGCCATC 
ACCCTGCGCAGCTTCCTCCGAGACTCGCGCCGGCCGATCGTCTTCGTACCGGTCTACATCGGCTACGAACGC 
GTGCTGGAAGGCCGCACCTACCTCGGCGAACTGCGCGGCGCGACCAAGAAGAAGGAGTCGATCTTCGACCTG 
TTCAAGGTGGTCGGCGCCCTGAAACAGCGTTTCGGCCAGGTCTGGGTGAACTTCGGCGAGCCGATCCATCTC 
GACCAGTTCCTCGACCGCCACCAGCCCGACTGGCAGGACCAGGACCTCGGCCCGGAATACCGCCCCGACTGG 
CTGCCGCAGACCACCAACCTGCTGGCCAAGGACGTCGCTCGCCACCTCAACGACGCGGCGGCGATCAACCCG 
GTCAACCTGGTCGCCCTGGCGCTGCTCTCCACCAGCCGCCAGGCCCTCGACGAAAGCGCCCTGGCGCGAATC 
CTCGACCTCTACCTGGCGCTGCTGCGCAAGGTGCCCTACTCGCCCAGCGCGACCCTGCCGGACGGCGACGGC 
CAGGCCCTGATCGAGTACGTGAAGAGCATGAACCTGCTGGCCGAGCAGAAGGACGCGCTGGGCCGCATCCTC 
TATCTCGACGAACAGAACGCGGTGCTGGCGACCTACTACCGCAACAACGTCCTCCACGTCTTCGCCCTGCCG 
GCGCTGATCGCCAGCTTCTTCCAGAGCAACTCGCGGATCAGCCGCGAACAGTTGCTGCGCTTCGCCCGCGCG 
CTCTATCCCTACCTGCAGGCGGAACTGTTCATCCGCTGGAGCCTGGACGAACTCGACGCGGTGATCGACCAG 
TGGCTGGCGGCCCTGGTGGAGCAGGGCCTGCTGCGCCAGGAGAACGACACCTTCATCCGCCCGGCGCCAAGC 
TCGCGGCAATACGTGCTGCTGATCCTCCTCGCTCGTTCGGTGACCCAGACCCTGCAGCGCTTCTACATGGCC 
ATCGCCCTGCTGCTCAACGCCGGGCAGAACGCCCTGACCGCCGAGGAACTGGAGAACCTCTGCACCGTGATG 
GCCCAGCGCCTGTCGATCCTGCATGGCCTGAACGCCCCGGAGTTCTTCGACAAGAGCCTGTTCCGCCACTTC 
ATCCAGACCCTTCTCGACCTGCGTGTGCTGCGCAAGGACGAGGCCGGCAAACTGAGCTACCACGAACTGCTC 
GGCGAACTGGCCGAAGGCGCCGCCAAGCGCGTGCTGCCGGCGGAGATCCGCCTGTCGATCCGCCAGGTGGCG 
CTGGAGCGTCCCGCCGAGGAAGCCGCCGCCGAAAGCAACGACGCAGCCGCCAACTGA