ViewGene
Locus Name:
GeneID:
Location:

GeneID:1566
TrivialName: PA14_08560
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_08560
PAO1 Ortholog Locus:PA0668
Sequence Length:1200
Protein Length:399
Start: 731347
Stop: 732546
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04057.1|AE004501_9 (AE004501) tyrosyl-tRNA synthetase 2 [Pseudomonas aeruginosa PAO1]
Identities = 399/399 (100%), Positives = 399/399 (100%)
Sequence:
ATGAAGTCGGTTGAAGAGCAGCTGGCGCTGATCCAGCGCGGTGCAGACGAGATCCTGGTAGAGGCGGAACTG
GTGGCCAAGCTGAAGCGTGGCCAGCCCTTGCGGATCAAGGCCGGCTTCGACCCGACCGCTCCCGATCTGCAT
CTTGGCCATACCGTGCTTATTAATAAGCTGCGCCAGTTCCAGGACCTGGGGCATCAGGTGATCTTCCTCATC
GGTGACTTCACTGGGATGATCGGTGACCCCAGTGGGAAAAGTGTTACCCGTCCTCCCCTGACGCGCGAGCAG
GTTCTGGAGAATGCGGAGACGTACAAGTCCCAGGTCTTCAAGATCCTCGACCCGGCCAAGACCGAAGTAGCC
TTCAATTCCACCTGGATGGACCAGCTGACCCCGGCTGACTTCATTCGTCTTGCCTCTCAGTACACCGTTGCG
CGCATGCTGGAGCGCGATGACTTTAGCAAGCGCTATGCCAGCAATCAGCCCATCGCTATCCATGAGTTCCTC
TATCCGCTGGTCCAGGGCTATGACTCCGTCGCGCTGAAGGCGGACGTCGAGTTGGGTGGCACCGACCAGAAG
TTCAACCTGCTGATGGGGCGCGAGCTGCAGCGCGCCTATGGTCAGGAGGCCCAGGTGATCCTGACCATGCCG
TTGCTGGAAGGGCTGGATGGCGTGAAGAAGATGTCCAAGTCGCTGGGCAACTATATTGGTATCCAGGAAGCG
CCGGGTGTCATGTACAGCAAGCTGGTCTCCATTCCTGACACGCTGATGTGGCGTTACTTCGAACTGCTCAGC
TTCCGTTCCCTGGATGAGATCGATAGCTTCCGCAAGGACGTCGAGGCGGGCGCCAACCCGCGTGACATCAAG
ATCAAGCTGGCCGAGGAGATCGTCGCCCGCTTCCATGGCGAGGAGGCGGCGGCGAGCGCACACAAGTCGGCC
GGCAATCGCCTGAAGGAGGGCGAGCTGCCGGAGGACCTGCCGGAGATCGAGCTGTCGTCCCCCGAGGATATG
CCCGTTGCCTCTGTCCTTAATAAGGCGGGGTTGGTGAAGAATGCGGCTGCGGCTCGCGATCTCCTGGGTGCC
GGCAGCGTCAAGGTGGATGGGCAGGTCGTCGACCGCACCTTCATGCTGGCGCTGGGTGAAACCCGTGTGTTC
CAGGCAGGGAAGAAGGCTTTCGCGCGCATCACGCTGAAAGCTGAATAA
Translation:
MKSVEEQLALIQRGADEILVEAELVAKLKRGQPLRIKAGFDPTAPDLHLGHTVLINKLRQFQDLGHQVIFLI
GDFTGMIGDPSGKSVTRPPLTREQVLENAETYKSQVFKILDPAKTEVAFNSTWMDQLTPADFIRLASQYTVA
RMLERDDFSKRYASNQPIAIHEFLYPLVQGYDSVALKADVELGGTDQKFNLLMGRELQRAYGQEAQVILTMP
LLEGLDGVKKMSKSLGNYIGIQEAPGVMYSKLVSIPDTLMWRYFELLSFRSLDEIDSFRKDVEAGANPRDIK
IKLAEEIVARFHGEEAAASAHKSAGNRLKEGELPEDLPEIELSSPEDMPVASVLNKAGLVKNAAAARDLLGA
GSVKVDGQVVDRTFMLALGETRVFQAGKKAFARITLKAE*
AnnotationID:1575GeneID:1566
AnnotatorUID: diggins
Modification Date/Time: 2005-03-29 19:07:55
Gene Name:tyrZ
Confidence Code:2
GeneProduct:tyrosyl-tRNA synthetase 2
Cell Localization:()
Synonyms:tyrS
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0162,cd00805,pfam00579
Secondary Functional Category(ies):translation, ribosomal structure and biogenesis
EC Number:6.1.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66066.1|AE016235_5 (AE016775) tyrosyl-tRNA synthetase [Pseudomonas putida KT2440]
          Length = 399

 Score =  701 bits (1809), Expect = 0.0
 Identities = 349/399 (87%), Positives = 378/399 (94%)

>gb|AAO54151.1| (AE016858) tyrosyl-tRNA synthetase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 403

 Score =  690 bits (1781), Expect = 0.0
 Identities = 344/399 (86%), Positives = 376/399 (94%)

>gb|AAN54380.1|AE015575_6 (AE015575) tyrosyl-tRNA synthetase [Shewanella oneidensis MR-1]
          Length = 398

 Score =  518 bits (1335), Expect = e-146
 Identities = 255/397 (64%), Positives = 322/397 (81%), Gaps = 1/397 (0%)








 
Structural Features:
COG0162, TyrS, Tyrosyl-tRNA synthetase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 401 residues, 100.0% aligned
Score =  427 bits (1099), Expect = 1e-120
Identities = 187/405 (46%), Positives = 237/405 (58%), Gaps = 12/405 (2%)

cd00805, TyrRS_core, Tyrosinyl-tRNA synthetase (TyrRS) catalytic core domain. 
CD-Length = 268 residues,  99.6% aligned
Score =  300 bits (770), Expect = 2e-82

pfam00579, tRNA-synt_1b, tRNA synthetases class I (W and Y).
CD-Length = 287 residues, 100.0% aligned
Score =  252 bits (646), Expect = 4e-68
Identities = 109/296 (36%), Positives = 160/296 (54%), Gaps = 16/296 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3135




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20282




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34619




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1566_cdd.html


    		




 
URL:

















    ReferenceID:35244




  
Author/Investigator(s):
        Salazar O, Sagredo B, Jedlicki E, Soll D, Weygand-Durasevic I, Orellana O.





  
Title:
        Thiobacillus ferrooxidans tyrosyl-tRNA synthetase functions in vivo in Escherichia coli.




  
PubMed:
        7517395


MedLine:
        




    
Source:
J Bacteriol. 1994 Jul;176(14):4409-15.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7517395









           
Homologs By Global Alignment 

           

       



     
Gene ID:1566
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2781
                           PA0668_tr
                           translation of PA0668
                           399
                           99.99
                           99.99
                           0
                           2003.0
     

     
     
87183
                           gb|AAG04057.1|AE004501_9
                           (AE004501) tyrosyl-tRNA synthetase 2 [Pseudomonas aeruginosa PAO1]
                           399
                           99.99
                           99.99
                           0
                           2003.0
     

     
     
87184
                           gb|AAN66066.1|AE016235_5
                           (AE016775) tyrosyl-tRNA synthetase [Pseudomonas putida KT2440]
                           399
                           87.46
                           94.73
                           0
                           1809.0
     

     
     
87185
                           gb|AAO54151.1|
                           (AE016858) tyrosyl-tRNA synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           403
                           85.35
                           93.30
                           4
                           1781.0
     

     
     
87186
                           gb|AAN54380.1|AE015575_6
                           (AE015575) tyrosyl-tRNA synthetase [Shewanella oneidensis MR-1]
                           400
                           63.75
                           80.50
                           3
                           1335.0
     

     






      

          
GC ORFID: 44568How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0668Glimmer Score: 

          
Start: 6532690Stop: 6533889

          
Length: 1200


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA0668 translation of PA0668Homolog Bit Score 776.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2127 (ORF: BLASTX 692.0), t_PA0668 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGTCGGTTGAAGAGCAGCTGGCGCTGATCCAGCGCGGTGCAGACGAGATCCTGGTAGAGGCGGAACTG 
GTGGCCAAGCTGAAGCGTGGCCAGCCCTTGCGGATCAAGGCCGGCTTCGACCCGACCGCTCCCGATCTGCAT 
CTTGGCCATACCGTGCTTATTAATAAGCTGCGCCAGTTCCAGGACCTGGGGCATCAGGTGATCTTCCTCATC 
GGTGACTTCACTGGGATGATCGGTGACCCCAGTGGGAAAAGTGTTACCCGTCCTCCCCTGACGCGCGAGCAG 
GTTCTGGAGAATGCGGAGACGTACAAGTCCCAGGTCTTCAAGATCCTCGACCCGGCCAAGACCGAAGTAGCC 
TTCAATTCCACCTGGATGGACCAGCTGACCCCGGCTGACTTCATTCGTCTTGCCTCTCAGTACACCGTTGCG 
CGCATGCTGGAGCGCGATGACTTTAGCAAGCGCTATGCCAGCAATCAGCCCATCGCTATCCATGAGTTCCTC 
TATCCGCTGGTCCAGGGCTATGACTCCGTCGCGCTGAAGGCGGACGTCGAGTTGGGTGGCACCGACCAGAAG 
TTCAACCTGCTGATGGGGCGCGAGCTGCAGCGCGCCTATGGTCAGGAGGCCCAGGTGATCCTGACCATGCCG 
TTGCTGGAAGGGCTGGATGGCGTGAAGAAGATGTCCAAGTCGCTGGGCAACTATATTGGTATCCAGGAAGCG 
CCGGGTGTCATGTACAGCAAGCTGGTCTCCATTCCTGACACGCTGATGTGGCGTTACTTCGAACTGCTCAGC 
TTCCGTTCCCTGGATGAGATCGATAGCTTCCGCAAGGACGTCGAGGCGGGCGCCAACCCGCGTGACATCAAG 
ATCAAGCTGGCCGAGGAGATCGTCGCCCGCTTCCATGGCGAGGAGGCGGCGGCGAGCGCACACAAGTCGGCC 
GGCAATCGCCTGAAGGAGGGCGAGCTGCCGGAGGACCTGCCGGAGATCGAGCTGTCGTCCCCCGAGGATATG 
CCCGTTGCCTCTGTCCTTAATAAGGCGGGGTTGGTGAAGAATGCGGCTGCGGCTCGCGATCTCCTGGGTGCC 
GGCAGCGTCAAGGTGGATGGGCAGGTCGTCGACCGCACCTTCATGCTGGCGCTGGGTGAAACCCGTGTGTTC 
CAGGCAGGGAAGAAGGCTTTCGCGCGCATCACGCTGAAAGCTGAATAA