ViewGene
Locus Name:
GeneID:
Location:

GeneID:1553
TrivialName: PA14_65770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_65770
PAO1 Ortholog Locus:PA4976
Sequence Length:1182
Protein Length:393
Start: 5858947
Stop: 5857766
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08361.1|AE004910_8 (AE004910) aspartate transaminase [Pseudomonas aeruginosa PAO1]
Identities = 389/393 (98%), Positives = 392/393 (99%)
Sequence:
ATGCGCTATTCCGACTTCACCCAACGTATCGCCGGCGACGGCGCCGCTGCCTGGGACATCCACTATCGCGCG
CTGGCGCGGGTCGAGCAGGGCGAGGAAATCCTCCTGCTGTCGGTCGGCGACCCCGACTTCGATACCCCGGCG
CCCATCGTCCAGTCGGCCATCGACAGCCTGCTGGCCGGCAACACCCACTACGCCGACGTGCGCGGCAAGCGC
GCCCTGCGCCAGCGCATCGCCGAACGCCACCGACAGCGCAGCGGCCAGGCGGTGGACGCGGAGCAGGTGGTG
GTGCTGGCCGGCGCCCAGTGCGCGCTGTACGCGGTGGTGCAGTGCCTGCTGAATCCGGGCGACGAGGTGATA
GTCGCCGAGCCGATGTACGTCACCTACGAAGCGGTGTTCGGCGCCTGTGGCGCGCGGGTGGTGCCGGTGCCG
GTGCGCTCCGAGAATGGCTTCCGCGTGCAGGCCGAGGAGGTCGCCGCACTGATCACCCCGCGCACGCGCGCG
ATGGCGCTGAACAGCCCGCACAACCCGTCCGGCGCCAGCCTGCCGCGGGCCACCTGGGAAGCCCTGGCCGAG
CTGTGCACGGCCCATGACCTGTGGATGATCTCCGACGAGGTCTACAGCGAACTGCTGTTCGACGGCGAGCAC
GTCAGCCCGGCCAGCCTGCCGGGGATGGCCGAACGTACCGCGACCCTCAACAGCCTGTCGAAATCCCACGCC
ATGACCGGCTGGCGGGTCGGCTGGGTGGTCGGTCCCGCGGCGCTCTGCGCGCACCTGGAGAACCTCGCCCTG
TGCATGCTCTATGGCTCGCCGGAATTCATCCAGGATGCCGCCTGCACCGCGCTGGAGGCGCCGCTGCCGGAG
TTGGAGGCGATGCGCGAGGCCTACCGCCGCCGCCGCGACCTGGTAATCGAGTGCCTGGCCGACAGCCCCGGC
CTGCGTCCACTGCGCCCGGACGGCGGCATGTTCGTGATGGTCGACATCCGTCCCACCGGACTTTCCGCCCAG
GCCTTCGCCGACCGCCTGCTGGACCGCCATGGCGTATCGGTGCTCGCCGGCGAAGCCTTCGGCCCGAGCGCC
GCCGGTCACATCCGCCTCGGCCTGGTGTTGGGCGCCGAACCGCTGCGCGAAGCCTGTCGGCGCATCGCGCTG
TGCGCCGCCGAGTTGCTCGGCCAGGCCTGA
Translation:
MRYSDFTQRIAGDGAAAWDIHYRALARVEQGEEILLLSVGDPDFDTPAPIVQSAIDSLLAGNTHYADVRGKR
ALRQRIAERHRQRSGQAVDAEQVVVLAGAQCALYAVVQCLLNPGDEVIVAEPMYVTYEAVFGACGARVVPVP
VRSENGFRVQAEEVAALITPRTRAMALNSPHNPSGASLPRATWEALAELCTAHDLWMISDEVYSELLFDGEH
VSPASLPGMAERTATLNSLSKSHAMTGWRVGWVVGPAALCAHLENLALCMLYGSPEFIQDAACTALEAPLPE
LEAMREAYRRRRDLVIECLADSPGLRPLRPDGGMFVMVDIRPTGLSAQAFADRLLDRHGVSVLAGEAFGPSA
AGHIRLGLVLGAEPLREACRRIALCAAELLGQA*
AnnotationID:1562GeneID:1553
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 08:08:36
Gene Name:aspC
Confidence Code:2
GeneProduct:aspartate transaminase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0436,COG1167,COG1168,COG1168
Secondary Functional Category(ies):transport of small molecules
EC Number:2.6.1.1
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69318.1|AE016569_2 (AE016788) aspartate aminotransferase [Pseudomonas putida KT2440]
          Length = 396

 Score =  637 bits (1644), Expect = 0.0
 Identities = 317/390 (81%), Positives = 346/390 (88%)








 
Structural Features:
COG0436, COG0436, Aspartate/tyrosine/aromatic aminotransferase 
[Amino acid transport and metabolism]
CD-Length = 393 residues,  99.7% aligned
Score =  360 bits (925), Expect = 2e-100
Identities = 164/393 (41%), Positives = 234/393 (59%), Gaps = 9/393 (2%)

COG1167, ARO8, Transcriptional regulators containing a DNA-binding HTH domain and 
an aminotransferase domain (MocR family) and their eukaryotic orthologs 
[Transcription / Amino acid transport and metabolism]
CD-Length = 459 residues,  81.9% aligned
Score =  179 bits (456), Expect = 4e-46
Identities = 112/380 (29%), Positives = 173/380 (45%), Gaps = 18/380 (4%)

COG1168, MalY, Bifunctional PLP-dependent enzyme with beta-cystathionase and 
maltose regulon repressor activities 
[Amino acid transport and metabolism]
CD-Length = 388 residues,  97.9% aligned
Score =  166 bits (421), Expect = 5e-42
Identities = 102/389 (26%), Positives = 169/389 (43%), Gaps = 18/389 (4%)

COG0079, HisC, Histidinol-phosphate/aromatic aminotransferase and 
cobyric acid decarboxylase 
[Amino acid transport and metabolism]
CD-Length = 356 residues,  85.7% aligned
Score =  131 bits (331), Expect = 1e-31
Identities = 93/328 (28%), Positives = 137/328 (41%), Gaps = 25/328 (7%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3109




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20256




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35117




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1553_cdd.html


    		




 
URL:

















    ReferenceID:35880




  
Author/Investigator(s):
        Kuramitsu S, Okuno S, Ogawa T, Ogawa H, Kagamiyama H.





  
Title:
        Aspartate aminotransferase of Escherichia coli: nucleotide sequence of the aspC gene.




  
PubMed:
        3897210


MedLine:
        




    
Source:
J Biochem (Tokyo). 1985 Apr;97(4):1259-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3897210

















    ReferenceID:35881




  
Author/Investigator(s):
        Fotheringham IG, Dacey SA, Taylor PP, Smith TJ, Hunter MG, Finlay ME, Primrose SB, Parker DM, Edwards RM.





  
Title:
        The cloning and sequence analysis of the aspC and tyrB genes from Escherichia coli K12. Comparison of the primary structures of the aspartate aminotransferase and aromatic aminotransferase of E. coli with those of the pig aspartate aminotransferase isoenz




  
PubMed:
        3521591


MedLine:
        




    
Source:
Biochem J. 1986 Mar 15;234(3):593-604.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3521591









           
Homologs By Global Alignment 

           

       



     
Gene ID:1553
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2756
                           PA4976_tr
                           translation of PA4976
                           393
                           98.98
                           99.74
                           0
                           2008.0
     

     
     
86555
                           gb|AAG08361.1|AE004910_8
                           (AE004910) aspartate transaminase [Pseudomonas aeruginosa PAO1]
                           393
                           98.98
                           99.74
                           0
                           2008.0
     

     
     
86556
                           gb|AAN69318.1|AE016569_2
                           (AE016788) aspartate aminotransferase [Pseudomonas putida KT2440]
                           396
                           80.55
                           87.87
                           3
                           1645.0
     

     
     
86558
                           dbj|BAB48694.1|
                           (AP002997) aspartate transaminase [Mesorhizobium loti]
                           395
                           41.01
                           56.96
                           4
                           763.0
     

     
     
86557
                           emb|CAC45138.1|
                           (AL591784) PUTATIVE AMINOTRANSFERASE PROTEIN [Sinorhizobium meliloti]
                           397
                           39.29
                           57.43
                           5
                           762.0
     

     






      

          
GC ORFID: 44555How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4976Glimmer Score: 

          
Start: 5122629Stop: 5121448

          
Length: 1182


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4976 translation of PA4976Homolog Bit Score 778.0

          
Other Homologs:  t_PA4976 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCTATTCCGACTTCACCCAACGTATCGCCGGCGACGGCGCCGCTGCCTGGGACATCCACTATCGCGCG 
CTGGCGCGGGTCGAGCAGGGCGAGGAAATCCTCCTGCTGTCGGTCGGCGACCCCGACTTCGATACCCCGGCG 
CCCATCGTCCAGTCGGCCATCGACAGCCTGCTGGCCGGCAACACCCACTACGCCGACGTGCGCGGCAAGCGC 
GCCCTGCGCCAGCGCATCGCCGAACGCCACCGACAGCGCAGCGGCCAGGCGGTGGACGCGGAGCAGGTGGTG 
GTGCTGGCCGGCGCCCAGTGCGCGCTGTACGCGGTGGTGCAGTGCCTGCTGAATCCGGGCGACGAGGTGATA 
GTCGCCGAGCCGATGTACGTCACCTACGAAGCGGTGTTCGGCGCCTGTGGCGCGCGGGTGGTGCCGGTGCCG 
GTGCGCTCCGAGAATGGCTTCCGCGTGCAGGCCGAGGAGGTCGCCGCACTGATCACCCCGCGCACGCGCGCG 
ATGGCGCTGAACAGCCCGCACAACCCGTCCGGCGCCAGCCTGCCGCGGGCCACCTGGGAAGCCCTGGCCGAG 
CTGTGCACGGCCCATGACCTGTGGATGATCTCCGACGAGGTCTACAGCGAACTGCTGTTCGACGGCGAGCAC 
GTCAGCCCGGCCAGCCTGCCGGGGATGGCCGAACGTACCGCGACCCTCAACAGCCTGTCGAAATCCCACGCC 
ATGACCGGCTGGCGGGTCGGCTGGGTGGTCGGTCCCGCGGCGCTCTGCGCGCACCTGGAGAACCTCGCCCTG 
TGCATGCTCTATGGCTCGCCGGAATTCATCCAGGATGCCGCCTGCACCGCGCTGGAGGCGCCGCTGCCGGAG 
TTGGAGGCGATGCGCGAGGCCTACCGCCGCCGCCGCGACCTGGTAATCGAGTGCCTGGCCGACAGCCCCGGC 
CTGCGTCCACTGCGCCCGGACGGCGGCATGTTCGTGATGGTCGACATCCGTCCCACCGGACTTTCCGCCCAG 
GCCTTCGCCGACCGCCTGCTGGACCGCCATGGCGTATCGGTGCTCGCCGGCGAAGCCTTCGGCCCGAGCGCC 
GCCGGTCACATCCGCCTCGGCCTGGTGTTGGGCGCCGAACCGCTGCGCGAAGCCTGTCGGCGCATCGCGCTG 
TGCGCCGCCGAGTTGCTCGGCCAGGCCTGA