ViewGene
Locus Name:
GeneID:
Location:

GeneID:1552
TrivialName: PA14_25090
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25090
PAO1 Ortholog Locus:PA3013
Sequence Length:1176
Protein Length:391
Start: 2194928
Stop: 2196103
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06401.1|AE004726_7 (AE004726) fatty-acid oxidation complex beta-subunit [Pseudomonas aeruginosa PAO1]
Identities = 389/391 (99%), Positives = 391/391 (100%)
Sequence:
ATGAGCCTGAATCCGAGAGACGTCGTCATTGTCGACTTCGGCCGCACCCCGATGGGTCGTTCCAAGGGTGGC
ATGCACCGCAACACTCGCGCCGAGACCATGTCGGCGCACCTGATCAGCAAGCTGCTGGAGCGTAACCCGAAG
GTCGACCCGGCCGAAGTGGAAGACGTGATCTGGGGTTGCGTCAACCAGACCCTGGAACAGGGCTGGAACATC
GCGCGCATGGCGTCGCTGATGACCCAGATCCCGCACACCAGCGCCGCGCAGACCGTCAGCCGCCTGTGCGGT
TCGTCGATGAGCGCGCTGCACACCGCCGCCCAGGCGATCCAGACCGGCAATGGCGATGTATTCGTCATCGGC
GGCGTGGAGCACATGGGCCACGTCGGCATGATGCATGGCGTCGACCCGAACCCGCACCTGTCCCTGTACGCC
GCCAAGGCCTCGGGCATGATGGGCCTGACCGCGGAAATGCTCGGCAAGATGCACGGTATCAGCCGTGAGGCG
CAGGACAAGTTCGGCGCGCGTTCGCACCAACTGGCCTGGAAGGCCACCCAGGAAGGCAAGTTCAAGGACGAG
ATCATCCCGATGGAAGGGTATGACGAGAACGGTTTCCTGAAAGTCTTCGACTTCGACGAGACCATTCGTCCG
GAAACCACCGTCGAGACCCTGGCCCAGTTGAAGCCGGCGTTCAATCCGAAAGGCGGCACCGTGACCGCGGGG
ACCTCCTCGCAGATCACCGACGGTGCCTCCTGCATGATCGTGATGTCGGCCCAGCGCGCCCAGGACCTGGGC
ATCCAGCCGATGGCGGTGATCCGCTCCATGGCCGTGGCGGGTGTCGACCCGGCGATCATGGGCTACGGCCCG
GTGCCGTCGACCAACAAGGCGCTCAAGCGCGCCGGCCTGACCATCGCCGACATCGATTTCGTCGAACTCAAC
GAAGCCTTCGCCGCCCAGGCCCTGCCGGTGCTGAAGGACCTCAAGCTGCTCGACAAGATGGACGAGAAGGTC
AACCTCCACGGCGGCGCCATTGCCCTGGGTCACCCGTTCGGTTGCTCCGGTGCACGCATTTCCGGCACCCTG
TTGAACGTGATGAAGCAGAACGGCGGTACCCTTGGCGTGTCCACCATGTGCGTGGGCCTGGGACAGGGTATC
ACCACCGTCTTCGAGCGCGTCTAA
Translation:
MSLNPRDVVIVDFGRTPMGRSKGGMHRNTRAETMSAHLISKLLERNPKVDPAEVEDVIWGCVNQTLEQGWNI
ARMASLMTQIPHTSAAQTVSRLCGSSMSALHTAAQAIQTGNGDVFVIGGVEHMGHVGMMHGVDPNPHLSLYA
AKASGMMGLTAEMLGKMHGISREAQDKFGARSHQLAWKATQEGKFKDEIIPMEGYDENGFLKVFDFDETIRP
ETTVETLAQLKPAFNPKGGTVTAGTSSQITDGASCMIVMSAQRAQDLGIQPMAVIRSMAVAGVDPAIMGYGP
VPSTNKALKRAGLTIADIDFVELNEAFAAQALPVLKDLKLLDKMDEKVNLHGGAIALGHPFGCSGARISGTL
LNVMKQNGGTLGVSTMCVGLGQGITTVFERV*
AnnotationID:1561GeneID:1552
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 18:06:08
Gene Name:foaB
Confidence Code:2
GeneProduct:fatty-acid oxidation complex beta-subunit
Cell Localization:()
Synonyms:fadA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:3-oxoacyl-CoA thiolase
Functional Category Confidence Code:5
COGs:cd00751,COG0183
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:2.3.1.16
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAK83059.1|AF288535_2 (AF288535) beta-ketothiolase FadA [Pseudomonas oleovorans]
          Length = 391

 Score =  732 bits (1890), Expect = 0.0
 Identities = 364/391 (93%), Positives = 376/391 (96%)

>gb|AAF02534.1|AF150672_1 (AF150672) 3-oxoacyl-CoA thiolase [Pseudomonas putida]
          Length = 391

 Score =  731 bits (1888), Expect = 0.0
 Identities = 366/391 (93%), Positives = 375/391 (95%)

>gb|AAL22826.1| (AE008886) 3-ketoacyl-CoA thiolase [Salmonella typhimurium LT2]
          Length = 387

 Score =  508 bits (1307), Expect = e-143
 Identities = 250/384 (65%), Positives = 307/384 (79%)






 
Structural Features:
cd00751, thiolase
CD-Length = 386 residues, 100.0% aligned
Score =  452 bits (1164), Expect = 4e-128

COG0183, PaaJ, Acetyl-CoA acetyltransferase [Lipid metabolism]
CD-Length = 392 residues, 100.0% aligned
Score =  339 bits (871), Expect = 3e-94
Identities = 177/404 (43%), Positives = 226/404 (55%), Gaps = 29/404 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3107




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20254




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36035




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1552_cdd.html


    		




 
URL:

















    ReferenceID:36479




  
Author/Investigator(s):
        Nakahigashi K, Inokuchi H.





  
Title:
        Nucleotide sequence of the fadA and fadB genes from Escherichia coli.




  
PubMed:
        2204034


MedLine:
        




    
Source:
Nucleic Acids Res. 1990 Aug 25;18(16):4937.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2204034

















    ReferenceID:36480




  
Author/Investigator(s):
        DiRusso CC.





  
Title:
        Primary sequence of the Escherichia coli fadBA operon, encoding the fatty acid-oxidizing multienzyme complex, indicates a high degree of homology to eucaryotic enzymes.




  
PubMed:
        1699931


MedLine:
        




    
Source:
J Bacteriol. 1990 Nov;172(11):6459-68.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1699931

















    ReferenceID:36483




  
Author/Investigator(s):
        Yang SY, Yang XY, Healy-Louie G, Schulz H, Elzinga M.





  
Title:
        Nucleotide sequence of the fadA gene. Primary structure of 3-ketoacyl-coenzyme A thiolase from Escherichia coli and the structural organization of the fadAB operon.




  
PubMed:
        2191949


MedLine:
        




    
Source:
J Biol Chem. 1990 Jun 25;265(18):10424-9.

Erratum in:
    * J Biol Chem. 1991 Aug 25;266(24):16255.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2191949

















    ReferenceID:36484




  
Author/Investigator(s):
        Yang SY, He Yang XY, Healy-Louie G, Schulz H, Elzinga M.





  
Title:
        Nucleotide sequence of the fadA gene. Primary structure of 3-ketoacyl-coenzyme A thiolase from Escherichia coli and the structural organization of the fadAB operon.




  
PubMed:
        1678742


MedLine:
        




    
Source:
J Biol Chem. 1991 Aug 25;266(24):16255.

Erratum for:
    * J Biol Chem. 1990 Jun 25;265(18):10424-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1678742









           
Homologs By Global Alignment 

           

       



     
Gene ID:1552
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2752
                           PA3013_tr
                           translation of PA3013
                           391
                           99.48
                           99.99
                           0
                           2010.0
     

     
     
86342
                           gb|AAG06401.1|AE004726_7
                           (AE004726) fatty-acid oxidation complex beta-subunit [Pseudomonas aeruginosa PAO1]
                           391
                           99.48
                           99.99
                           0
                           2010.0
     

     
     
86345
                           gb|AAF02534.1|AF150672_1
                           (AF150672) 3-oxoacyl-CoA thiolase [Pseudomonas putida]
                           391
                           93.60
                           95.90
                           0
                           1888.0
     

     
     
86344
                           gb|AAK18168.1|AF290949_2
                           (AF290949) FadA [Pseudomonas putida]
                           391
                           93.60
                           95.90
                           0
                           1888.0
     

     
     
86346
                           gb|AAN67750.1|AE016406_5
                           (AE016782) 3-oxoacyl-CoA thiolase [Pseudomonas putida KT2440]
                           391
                           93.35
                           95.90
                           0
                           1885.0
     

     






      

          
GC ORFID: 44554How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3013Glimmer Score: 

          
Start: 2758999Stop: 2757824

          
Length: 1176


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA3013 translation of PA3013Homolog Bit Score 778.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8045 (ORF: BLASTX 726.0), t_PA2940 (ORF: BLASTX 276.0), psyr_15may02_Scaffold15_revised_gene2524 (ORF: BLASTX 272.0), psyr_15may02_Scaffold5_revised_gene6193 (ORF: BLASTX 269.0), t_PA2001 (ORF: BLASTX 268.0), t_PA3454 (ORF: BLASTX 256.0), t_PA3013 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCTGAATCCGAGAGACGTCGTCATTGTCGACTTCGGCCGCACCCCGATGGGTCGTTCCAAGGGTGGC 
ATGCACCGCAACACTCGCGCCGAGACCATGTCGGCGCACCTGATCAGCAAGCTGCTGGAGCGTAACCCGAAG 
GTCGACCCGGCCGAAGTGGAAGACGTGATCTGGGGTTGCGTCAACCAGACCCTGGAACAGGGCTGGAACATC 
GCGCGCATGGCGTCGCTGATGACCCAGATCCCGCACACCAGCGCCGCGCAGACCGTCAGCCGCCTGTGCGGT 
TCGTCGATGAGCGCGCTGCACACCGCCGCCCAGGCGATCCAGACCGGCAATGGCGATGTATTCGTCATCGGC 
GGCGTGGAGCACATGGGCCACGTCGGCATGATGCATGGCGTCGACCCGAACCCGCACCTGTCCCTGTACGCC 
GCCAAGGCCTCGGGCATGATGGGCCTGACCGCGGAAATGCTCGGCAAGATGCACGGTATCAGCCGTGAGGCG 
CAGGACAAGTTCGGCGCGCGTTCGCACCAACTGGCCTGGAAGGCCACCCAGGAAGGCAAGTTCAAGGACGAG 
ATCATCCCGATGGAAGGGTATGACGAGAACGGTTTCCTGAAAGTCTTCGACTTCGACGAGACCATTCGTCCG 
GAAACCACCGTCGAGACCCTGGCCCAGTTGAAGCCGGCGTTCAATCCGAAAGGCGGCACCGTGACCGCGGGG 
ACCTCCTCGCAGATCACCGACGGTGCCTCCTGCATGATCGTGATGTCGGCCCAGCGCGCCCAGGACCTGGGC 
ATCCAGCCGATGGCGGTGATCCGCTCCATGGCCGTGGCGGGTGTCGACCCGGCGATCATGGGCTACGGCCCG 
GTGCCGTCGACCAACAAGGCGCTCAAGCGCGCCGGCCTGACCATCGCCGACATCGATTTCGTCGAACTCAAC 
GAAGCCTTCGCCGCCCAGGCCCTGCCGGTGCTGAAGGACCTCAAGCTGCTCGACAAGATGGACGAGAAGGTC 
AACCTCCACGGCGGCGCCATTGCCCTGGGTCACCCGTTCGGTTGCTCCGGTGCACGCATTTCCGGCACCCTG 
TTGAACGTGATGAAGCAGAACGGCGGTACCCTTGGCGTGTCCACCATGTGCGTGGGCCTGGGACAGGGTATC 
ACCACCGTCTTCGAGCGCGTCTAA