ViewGene
Locus Name:
GeneID:
Location:

GeneID:155
TrivialName: PA14_34970
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_34970
PAO1 Ortholog Locus:PA2290
Sequence Length:2412
Protein Length:803
Start: 3111856
Stop: 3114267
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05678.1|AE004654_9 (AE004654) glucose dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 801/803 (99%), Positives = 802/803 (99%)
Sequence:
ATGAGCGAAGGTAACCACAGTCGATCGCGCCTGCTGCCGAGCCTGCTCGGCCTGTTGATCCTGCTGTCCGGC
CTGGCGCTGCTGGCCGGCGGCATCAAGCTGGCGAGCCTGGGCGGCTCGCTGTACTACCTGCTGGCCGGCATC
GGCCTGGCCCTGAGCGGGCTGTTGCTGCTGCTGGGCAAGCGTGCGGCGCTAGGCCTGTACGCCCTGGTGCTG
TTCGCCAGCACCGTCTGGGCGCTGTGGGAAGTCGGCCTGGACTGGTGGCAACTGGTGCCGCGCCTGGCCCTG
TGGTTCGTCTTCGGCCTGCTGCTCTGGCTGCCCTGGTTCCGTCGTCCGCTGCTGGCCGAGGGGCCGGCGCCG
CTGGGCACCGCGGCGCTGGGCGTGGCGGTGGTCCTGGCCGGCGCCGCCGCGGTGGGCAGCCAGTTCACCAAT
CCGGGGCAGATCGTCGGTCGCATCGACCGCGACAGCGGCATGACCAGCACCGCCCCGGCGATGCCGGACGGC
GACTGGCAGGCCTACGGCCGCACCGAGTTCGGCGACCGCTACTCGCCGTTGAAGCAGATCACCCCGGCCAAC
GTCGGCCAGCTCGAGGAAGCCTGGCGCATCCGTACCGGCGACCTGCCGACCGCCGACGATCCGCTCGAACTG
ACCAACGAGAACACCCCGCTGAAGGTCAACGGCATGCTCTACGCCTGCACCGCGCACAGCAAGGTGCTGGCC
CTCGACCCGGACACCGGCGCGGAAATCTGGCGCTTCGACCCGCAGATCCAGAGCCCGGTAGGCTTCAAGGGC
TTCGCCCACATGACTTGCCGTGGCGTCTCCTACTACGACGAAGAGCAGTACGCCAGGAGCGACGTCGGCGCG
CCGCCGGCCGCGCTCTCCGAGGCCGGCAAGGCGGTCGCCGCCAGTTGTCCGCGGCGCCTGTTCCTGCCCACC
GCCGACGCCCGCCTGATCGCGATCAACGCCGACAACGGCAAGGTCTGCGAGGACTTCGGGGTCAAGGGCGCG
GTCGACCTGACGGCCGGCATCGGTCCGTTCACCCCGGGCGGCTACTACTCCACCTCGCCGGCGGCGGTCACC
CGCAATCTGGTGATCATCGGCGGCCACGTCACCGACAACGAGTCGACCAACGAGCCGTCCGGGGTGATCCGC
GCCTTCGACGTGCACGACGGCAAGCTGGTGTGGAACTGGGACAGCGGCAACCCGGACGAGACCGAGCCCCTG
GCTCCCGGCAAGTTCTATACCCGCAACTCGCCGAACATGTGGTCGCTGGCCAGCGTCGACGAGAAGCTCGGC
CAGGTCTACCTGCCGCTGGGCAACCAGATGCCCGACCAGTGGGGCGGCAACCGCACCCCCGGTGCCGAGAAG
TTCAGCGCCGGCCTGGTGGCTCTCGACCTGAACACCGGCAAGCTGCGCTGGAACTACCAGTTCACCCACCAC
GACCTGTGGGACATGGACGTCGGCAGCCAGCCGACCCTGCTCGACCTGAAGACCGCCGATGGCGTCAAGCCG
GCGCTGATCGCGCCGACCAAGCAGGGCAGCCTGTACGTGCTGGACCGCCGCGACGGCACGCCGATCGTGCCG
ATCCGCGAAGTGCCGGCGCCGCAGGGCGCGGTGGAGGGCGACCATACCGCGCCGACCCAGGCGCGTTCCGAC
CTCAACCTGCTGCGCCCGCCGCTGACCGAGCGCGACATGTGGGGCAGCAGCCCGTTCGACCAGATGCTCTGC
CGCATCCAGTTCCGCTCGCTGCGCTACGAGGGCCAGTACACCCCGCCGTCGGAGCAGGGCAGCCTGATCTAT
CCGGGCAACGTCGGGGTGTTCAACTGGGGCGGGGTGTCGGTCGACCCGGTGCGCCAGATCCTCTTCACCAGC
CCCAACTACATGGCCTTCGTCTCGCAGATGGTGCCGCGCGACAAGGTGCCCAGCGGAAGCAAGCGCGAGGGC
GAGACCAGCGGCGTGCAGCCGAACACCGGCGCGCCCTACGCGGTGATCATGCATCCGTTCATGTCGCCCATC
GGCCTGCCGTGCCAGGCGCCGTCCTGGGGTGACGTGGCGGGTATCGACCTGACCACCGCCAAGGTCGTCTGG
CAGCACAAGAACGGCACCAGCCGCGACAACACCCCGGTGCCGATCGGCCTCACCGTCGGCGTACCGAGCATG
GGCGGCTCGATCACCACCGCCGGCGGCGTGGCCTTCCTCAGCGGCACGCTCGACCAGTACCTGCGCGCCTAC
GACGTGAAGGACGGCAAGCAGCTCTGGCAGGCGCGCCTGCCGGCCGGCGGCCAGGCCACGCCGATGAGCTAC
ACCGGCAAGGACGGTCGCCAGTACGTGCTGATCGTCGCTGGCGGACACGGCTCCTTCGGCACCCGCATGGGC
GACTACATCATCGCCTACGCCCTGCCCAGGCAATGA
Translation:
MSEGNHSRSRLLPSLLGLLILLSGLALLAGGIKLASLGGSLYYLLAGIGLALSGLLLLLGKRAALGLYALVL
FASTVWALWEVGLDWWQLVPRLALWFVFGLLLWLPWFRRPLLAEGPAPLGTAALGVAVVLAGAAAVGSQFTN
PGQIVGRIDRDSGMTSTAPAMPDGDWQAYGRTEFGDRYSPLKQITPANVGQLEEAWRIRTGDLPTADDPLEL
TNENTPLKVNGMLYACTAHSKVLALDPDTGAEIWRFDPQIQSPVGFKGFAHMTCRGVSYYDEEQYARSDVGA
PPAALSEAGKAVAASCPRRLFLPTADARLIAINADNGKVCEDFGVKGAVDLTAGIGPFTPGGYYSTSPAAVT
RNLVIIGGHVTDNESTNEPSGVIRAFDVHDGKLVWNWDSGNPDETEPLAPGKFYTRNSPNMWSLASVDEKLG
QVYLPLGNQMPDQWGGNRTPGAEKFSAGLVALDLNTGKLRWNYQFTHHDLWDMDVGSQPTLLDLKTADGVKP
ALIAPTKQGSLYVLDRRDGTPIVPIREVPAPQGAVEGDHTAPTQARSDLNLLRPPLTERDMWGSSPFDQMLC
RIQFRSLRYEGQYTPPSEQGSLIYPGNVGVFNWGGVSVDPVRQILFTSPNYMAFVSQMVPRDKVPSGSKREG
ETSGVQPNTGAPYAVIMHPFMSPIGLPCQAPSWGDVAGIDLTTAKVVWQHKNGTSRDNTPVPIGLTVGVPSM
GGSITTAGGVAFLSGTLDQYLRAYDVKDGKQLWQARLPAGGQATPMSYTGKDGRQYVLIVAGGHGSFGTRMG
DYIIAYALPRQ*
AnnotationID:165GeneID:155
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 07:07:57
Gene Name:gcd
Confidence Code:2
GeneProduct: glucose dehydrogenase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4993,cd00216
Secondary Functional Category(ies):energy metabolism
EC Number:1.1.99.17
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67066.1|AE016335_6 (AE016779) glucose dehydrogenase (pyrroloquinoline-quinone)
           [Pseudomonas putida KT2440]
          Length = 803

 Score = 1323 bits (3424), Expect = 0.0
 Identities = 633/802 (78%), Positives = 710/802 (88%), Gaps = 6/802 (0%)

>dbj|BAB48858.1| (AP002997) glucose dehydrogenase [Mesorhizobium loti]
          Length = 785

 Score =  863 bits (2229), Expect = 0.0
 Identities = 438/803 (54%), Positives = 545/803 (67%), Gaps = 29/803 (3%)

>gb|AAL19133.1| (AE008702) glucose dehydrogenase [Salmonella typhimurium LT2]
          Length = 796

 Score =  787 bits (2033), Expect = 0.0
 Identities = 392/811 (48%), Positives = 535/811 (65%), Gaps = 29/811 (3%)








 
Structural Features:
COG4993, Gcd, Glucose dehydrogenase [Carbohydrate transport and metabolism]
CD-Length = 773 residues, 100.0% aligned
Score = 1057 bits (2735), Expect = 0.0
Identities = 470/796 (59%), Positives = 552/796 (69%), Gaps = 27/796 (3%)

cd00216, PQQ_DH, Dehydrogenases with pyrrolo-quinoline quinone (PQQ) as cofactor, 
like ethanol, methanol, and membrane bound glucose dehydrogenases.
CD-Length = 530 residues,  99.8% aligned
Score =  407 bits (1046), Expect = 4e-114
Identities = 215/658 (32%), Positives = 290/658 (44%), Gaps = 145/658 (22%)






   
Genomic Context:






   
Comment:


















    ReferenceID:313




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17455




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32497




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:155_cdd.html


    		




 
URL:

















    ReferenceID:34459




  
Author/Investigator(s):
        Yamada M, Asaoka S, Saier MH Jr, Yamada Y.





  
Title:
        Characterization of the gcd gene from Escherichia coli K-12 W3110 and regulation of its expression.




  
PubMed:
        8419307


MedLine:
        




    
Source:
J Bacteriol. 1993 Jan;175(2):568-71.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8419307

















    ReferenceID:34460




  
Author/Investigator(s):
        Cleton-Jansen AM, Goosen N, Fayet O, van de Putte P.





  
Title:
        Cloning, mapping, and sequencing of the gene encoding Escherichia coli quinoprotein glucose dehydrogenase.




  
PubMed:
        2228962


MedLine:
        




    
Source:
J Bacteriol. 1990 Nov;172(11):6308-15.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2228962









           
Homologs By Global Alignment 

           

       



     
Gene ID:155
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
281
                           PA2290_tr
                           translation of PA2290
                           803
                           99.75
                           99.87
                           0
                           4252.0
     

     
     
18297
                           gb|AAG05678.1|AE004654_9
                           (AE004654) glucose dehydrogenase [Pseudomonas aeruginosa PAO1]
                           803
                           99.75
                           99.87
                           0
                           4252.0
     

     
     
18298
                           gb|AAN67066.1|AE016335_6
                           (AE016779) glucose dehydrogenase (pyrroloquinoline-quinone) [Pseudomonas putida KT2440]
                           807
                           78.43
                           87.98
                           8
                           3432.0
     

     
     
18299
                           gb|AAO57652.1|
                           (AE016871) glucose dehydrogenase [Pseudomonas syringae pv. tomato str. DC3000]
                           806
                           65.63
                           78.03
                           62
                           2970.0
     

     
     
18300
                           dbj|BAB48858.1|
                           (AP002997) glucose dehydrogenase [Mesorhizobium loti]
                           815
                           54.11
                           67.23
                           42
                           2259.0
     

     






      

          
GC ORFID: 43157How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2290Glimmer Score: 

          
Start: 1842060Stop: 1839649

          
Length: 2412


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2290 translation of PA2290Homolog Bit Score 1642.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6147 (ORF: BLASTX 1217.0), psyr_15may02_Scaffold1_revised_gene1399 (ORF: BLASTX 648.0), t_PA2290 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGAAGGTAACCACAGTCGATCGCGCCTGCTGCCGAGCCTGCTCGGCCTGTTGATCCTGCTGTCCGGC 
CTGGCGCTGCTGGCCGGCGGCATCAAGCTGGCGAGCCTGGGCGGCTCGCTGTACTACCTGCTGGCCGGCATC 
GGCCTGGCCCTGAGCGGGCTGTTGCTGCTGCTGGGCAAGCGTGCGGCGCTAGGCCTGTACGCCCTGGTGCTG 
TTCGCCAGCACCGTCTGGGCGCTGTGGGAAGTCGGCCTGGACTGGTGGCAACTGGTGCCGCGCCTGGCCCTG 
TGGTTCGTCTTCGGCCTGCTGCTCTGGCTGCCCTGGTTCCGTCGTCCGCTGCTGGCCGAGGGGCCGGCGCCG 
CTGGGCACCGCGGCGCTGGGCGTGGCGGTGGTCCTGGCCGGCGCCGCCGCGGTGGGCAGCCAGTTCACCAAT 
CCGGGGCAGATCGTCGGTCGCATCGACCGCGACAGCGGCATGACCAGCACCGCCCCGGCGATGCCGGACGGC 
GACTGGCAGGCCTACGGCCGCACCGAGTTCGGCGACCGCTACTCGCCGTTGAAGCAGATCACCCCGGCCAAC 
GTCGGCCAGCTCGAGGAAGCCTGGCGCATCCGTACCGGCGACCTGCCGACCGCCGACGATCCGCTCGAACTG 
ACCAACGAGAACACCCCGCTGAAGGTCAACGGCATGCTCTACGCCTGCACCGCGCACAGCAAGGTGCTGGCC 
CTCGACCCGGACACCGGCGCGGAAATCTGGCGCTTCGACCCGCAGATCCAGAGCCCGGTAGGCTTCAAGGGC 
TTCGCCCACATGACTTGCCGTGGCGTCTCCTACTACGACGAAGAGCAGTACGCCAGGAGCGACGTCGGCGCG 
CCGCCGGCCGCGCTCTCCGAGGCCGGCAAGGCGGTCGCCGCCAGTTGTCCGCGGCGCCTGTTCCTGCCCACC 
GCCGACGCCCGCCTGATCGCGATCAACGCCGACAACGGCAAGGTCTGCGAGGACTTCGGGGTCAAGGGCGCG 
GTCGACCTGACGGCCGGCATCGGTCCGTTCACCCCGGGCGGCTACTACTCCACCTCGCCGGCGGCGGTCACC 
CGCAATCTGGTGATCATCGGCGGCCACGTCACCGACAACGAGTCGACCAACGAGCCGTCCGGGGTGATCCGC 
GCCTTCGACGTGCACGACGGCAAGCTGGTGTGGAACTGGGACAGCGGCAACCCGGACGAGACCGAGCCCCTG 
GCTCCCGGCAAGTTCTATACCCGCAACTCGCCGAACATGTGGTCGCTGGCCAGCGTCGACGAGAAGCTCGGC 
CAGGTCTACCTGCCGCTGGGCAACCAGATGCCCGACCAGTGGGGCGGCAACCGCACCCCCGGTGCCGAGAAG 
TTCAGCGCCGGCCTGGTGGCTCTCGACCTGAACACCGGCAAGCTGCGCTGGAACTACCAGTTCACCCACCAC 
GACCTGTGGGACATGGACGTCGGCAGCCAGCCGACCCTGCTCGACCTGAAGACCGCCGATGGCGTCAAGCCG 
GCGCTGATCGCGCCGACCAAGCAGGGCAGCCTGTACGTGCTGGACCGCCGCGACGGCACGCCGATCGTGCCG 
ATCCGCGAAGTGCCGGCGCCGCAGGGCGCGGTGGAGGGCGACCATACCGCGCCGACCCAGGCGCGTTCCGAC 
CTCAACCTGCTGCGCCCGCCGCTGACCGAGCGCGACATGTGGGGCAGCAGCCCGTTCGACCAGATGCTCTGC 
CGCATCCAGTTCCGCTCGCTGCGCTACGAGGGCCAGTACACCCCGCCGTCGGAGCAGGGCAGCCTGATCTAT 
CCGGGCAACGTCGGGGTGTTCAACTGGGGCGGGGTGTCGGTCGACCCGGTGCGCCAGATCCTCTTCACCAGC 
CCCAACTACATGGCCTTCGTCTCGCAGATGGTGCCGCGCGACAAGGTGCCCAGCGGAAGCAAGCGCGAGGGC 
GAGACCAGCGGCGTGCAGCCGAACACCGGCGCGCCCTACGCGGTGATCATGCATCCGTTCATGTCGCCCATC 
GGCCTGCCGTGCCAGGCGCCGTCCTGGGGTGACGTGGCGGGTATCGACCTGACCACCGCCAAGGTCGTCTGG 
CAGCACAAGAACGGCACCAGCCGCGACAACACCCCGGTGCCGATCGGCCTCACCGTCGGCGTACCGAGCATG 
GGCGGCTCGATCACCACCGCCGGCGGCGTGGCCTTCCTCAGCGGCACGCTCGACCAGTACCTGCGCGCCTAC 
GACGTGAAGGACGGCAAGCAGCTCTGGCAGGCGCGCCTGCCGGCCGGCGGCCAGGCCACGCCGATGAGCTAC 
ACCGGCAAGGACGGTCGCCAGTACGTGCTGATCGTCGCTGGCGGACACGGCTCCTTCGGCACCCGCATGGGC 
GACTACATCATCGCCTACGCCCTGCCCAGGCAATGA