ViewGene
Locus Name:
GeneID:
Location:

GeneID:154
TrivialName: PA14_41530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41530
PAO1 Ortholog Locus:PA1781
Sequence Length:2451
Protein Length:816
Start: 3706047
Stop: 3708497
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05170.1|AE004603_10 (AE004603) assimilatory nitrite reductase large subunit [Pseudomonas aeruginosa PAO1]
Identities = 812/816 (99%), Positives = 814/816 (99%)
Sequence:
ATGAAGAAGATCAAGCTGGTAATGGTCGGCAACGGCATGGCCGGGGTCCGTACCCTGGAAGAACTGCTCAAG
CTCAACTCGGACTTCTACGACATCACGGTGTTCGGCGCCGAGCCCCATCCCAACTACAACCGCATCCTGCTG
TCCCCGGTACTGGCCGGCGAGCAGACCTTCGAGGAAATCGTCCTCAACGACCTCAACTGGTATGCGGAAAAC
GGCATCAAGCTCCTCCTCGACCGCAAGGTGGTGCAGATCGACCGGGTCCGCCGCAGGGTGGTCGCCGCGGAC
GGCAGCGAGGCCGAGTACGACCGCCTGCTGCTGGCCACCGGCTCGGTCCCCTTCATCCTGCCGATACCCGGC
AATCGGCTGCAGGGCGTGATCGGCTACCGCGACATCGCCGACACCCAGGCCATGATCGACTGCGCCCGGACC
CACAGCCACGCGGTGGTGATCGGCGGCGGCCTGCTCGGCCTGGAGGCGGCCAACGGCCTCAAGCAACGCGGC
ATGGACGTGACCGTCGTGCACCTCTCCGACTGGCTGCTTGAACGCCAACTGGACCGCACCGCCGGCAAACTC
CTGCAAGGCGCGCTGGAAGCCCGCGGCATCCGCTTCCGCCTGAACACCCAGACCCAGGAACTGATGGACAAC
GGCAGCGGCCGGGTCTGTGCCGTGCAGTTCAACGACGGCGACGTGATTCCCGCCGACCTGGTGGTAATGGCC
GCCGGTATCCGCCCCAACACCGAGCTGGCCGAAAGCGCAGGCATCCCCTGCAACCGCGGCATCCTGGTCAAC
GACACCCTGCAGACCTACGACCCACGCATCTATGCGGTCGGCGAATGCGCCAACCACCGTGGCATCGCCTAC
GGCCTGGTTGCGCCGCTGTTCGAGCAGGCCAAGGTCTGCGCCAACCACCTGGCTCATCTCGGCTACGCCCGC
TACCAGGGCTCGGTGACCTCGACCAAGCTCAAGGTGACCGGTATCGACCTGTTCTCCGCCGGCGACTTCATC
GGCGGCGAAGGCAGCGAGACCATCACCCTTTCCGACCCCATCGGCGGCGTCTACAAGAAACTGGTGATCAAG
GACGATGTACTGGTCGGCGCCTGTCTCTACGGCGACACCGCCGACGGCGGCTGGTACTTCCGGCAGATCCGC
GAGAACCACAACGTCGCGCAGATCCGCGACCACCTGATGTTCGGCGAGAACGCCCTCGGCGATGTCGGCCAC
CAGGGCCAGAACAGCGCCGCGAGCATGCCCGACACGGCCGAAGTATGCGGCTGCAACGGCGTCTGCAAGGGC
ACCATCGTCAAGGCGATACAAGAACACGGCCTGTTCAGCGTCGACGAAGTCAAGAAACACACCAAGGCCGCC
AGCTCCTGCGGCTCCTGCGCGGGATTGGTGGAACAGATCCTGATCAGCACCGTCGGCGGCGCCGCCGACGTC
AAGCCGAAGAGCGAGAAGGCCATCTGCGGCTGCAGCGACCTCAACCACGGGCAGATCCGCAAGGCCATTCGC
GAACACCGGCTGACCAGTCTCGACGCCGTCATGCGCTTCATGGACTGGAAGACCCCGAACGGCTGTGCCACC
TGCCGCCCGGCCCTGAACTATTACCTGATCTCGACCTGGCCGGGAGAAGCCAAGGACGATCCGCAGTCGCGC
CTGATCAACGAACGCGCCCACGCCAACATCCAGAAGGACGGCACCTACTCGGTGGTACCGCGGATGTGGGGC
GGCGTGACCAACGCCGCCGAGTTGCGGCGCATCGCCGACGTCGCCGACAAGTACCAGGTGCCGATGGTCAAG
GTCACCGGTGGCCAGCGCATCGACCTGCTGGGGGTGAAGAAGGACGACCTGCCGGCCATCTGGAAAGACCTC
GACATGCCCTCCGGACATGCCTACGGCAAATCCATCCGCACCGTGAAGACCTGCGTCGGCAGCGAGTTCTGC
CGCTTCGGCACGCAAAACTCGACGCAGCTGGGCATCGATCTGGAACACGACCTGTTCAACATGTGGTCGCCG
CACAAGGTCAAGCTGGCGGTCTCCGGCTGCCCGCGCAACTGCGCCGAGGCCGGGATCAAGGACGTCGGCATC
ATCGGCGTGGACTCCGGCTGGGAGATGTACATCGGCGGCAACGGCGGGATCAAGACCGAGGTCGCGGAGTTC
TTCGTCAAGCTCAAGACCGCCGAAGAAGTGCGCGAATACAACGGCGCCTTCCTCCAGCTCTACCGCGAGGAA
GCCTTCTACCTGGAGCGCACGGTGCATTACCTGCAGCGGGTCGGCATGGAGCACATCCGCAAGGCGGTGGTC
GAGGACGCGGAAAACCGCAAGGCGCTCAACGACCGCCTGCAATTCGCCCTGTCCTTCGAGCAGGACCCATGG
AAGCAGCGCATCGAACAGCCGCAATTGAAGAAGGAGTTCGAACGGATTCCGCTCAAGCAACTGGAAAACGTC
TGA
Translation:
MKKIKLVMVGNGMAGVRTLEELLKLNSDFYDITVFGAEPHPNYNRILLSPVLAGEQTFEEIVLNDLNWYAEN
GIKLLLDRKVVQIDRVRRRVVAADGSEAEYDRLLLATGSVPFILPIPGNRLQGVIGYRDIADTQAMIDCART
HSHAVVIGGGLLGLEAANGLKQRGMDVTVVHLSDWLLERQLDRTAGKLLQGALEARGIRFRLNTQTQELMDN
GSGRVCAVQFNDGDVIPADLVVMAAGIRPNTELAESAGIPCNRGILVNDTLQTYDPRIYAVGECANHRGIAY
GLVAPLFEQAKVCANHLAHLGYARYQGSVTSTKLKVTGIDLFSAGDFIGGEGSETITLSDPIGGVYKKLVIK
DDVLVGACLYGDTADGGWYFRQIRENHNVAQIRDHLMFGENALGDVGHQGQNSAASMPDTAEVCGCNGVCKG
TIVKAIQEHGLFSVDEVKKHTKAASSCGSCAGLVEQILISTVGGAADVKPKSEKAICGCSDLNHGQIRKAIR
EHRLTSLDAVMRFMDWKTPNGCATCRPALNYYLISTWPGEAKDDPQSRLINERAHANIQKDGTYSVVPRMWG
GVTNAAELRRIADVADKYQVPMVKVTGGQRIDLLGVKKDDLPAIWKDLDMPSGHAYGKSIRTVKTCVGSEFC
RFGTQNSTQLGIDLEHDLFNMWSPHKVKLAVSGCPRNCAEAGIKDVGIIGVDSGWEMYIGGNGGIKTEVAEF
FVKLKTAEEVREYNGAFLQLYREEAFYLERTVHYLQRVGMEHIRKAVVEDAENRKALNDRLQFALSFEQDPW
KQRIEQPQLKKEFERIPLKQLENV*
AnnotationID:164GeneID:154
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 13:01:41
Gene Name:nirB
Confidence Code:2
GeneProduct:assimilatory nitrite reductase large subunit
Cell Localization:()
Synonyms:nasD
Cell Localization Confidence Code:5
MolecularFunction:nitrite reductase [NAD(P)H]
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1251,pfam00070
Secondary Functional Category(ies):energy production and conversion
EC Number:1.6.6.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM41296.1| (AE012305) nitrite reductase [NAD(P)H] [Xanthomonas campestris pv.
           campestris str. ATCC 33913]
          Length = 817

 Score = 1222 bits (3161), Expect = 0.0
 Identities = 594/796 (74%), Positives = 683/796 (85%), Gaps = 1/796 (0%)

>gb|AAP30018.1| (AY163854) assimilatory nitrite reductase large subunit
           [Pseudomonas chlororaphis]
          Length = 589

 Score = 1052 bits (2720), Expect = 0.0
 Identities = 507/589 (86%), Positives = 552/589 (93%)

>gb|AAM73544.1|AF283498_4 (AF283498) assimilatory nitrite reductase large subunit
           [Azospirillum brasilense]
          Length = 821

 Score =  971 bits (2509), Expect = 0.0
 Identities = 484/813 (59%), Positives = 605/813 (74%), Gaps = 9/813 (1%)








 
Structural Features:
COG1251, NirB, NAD(P)H-nitrite reductase [Energy production and conversion]
CD-Length = 793 residues, 100.0% aligned
Score = 1129 bits (2923), Expect = 0.0
Identities = 490/808 (60%), Positives = 588/808 (72%), Gaps = 15/808 (1%)

pfam00070, Pyr_redox, Pyridine nucleotide-disulphide oxidoreductase. 
CD-Length = 288 residues,  97.9% aligned
Score =  193 bits (491), Expect = 7e-50
Identities = 104/290 (35%), Positives = 146/290 (50%), Gaps = 18/290 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:311




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17453




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37143




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:154_cdd.html


    		




 
URL:

















    ReferenceID:37813




  
Author/Investigator(s):
        Peakman T, Crouzet J, Mayaux JF, Busby S, Mohan S, Harborne N, Wootton J, Nicolson R, Cole J.





  
Title:
        Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.




  
PubMed:
        2200672


MedLine:
        




    
Source:
Eur J Biochem. 1990 Jul 31;191(2):315-23.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2200672

















    ReferenceID:37818




  
Author/Investigator(s):
        Ogawa K, Akagawa E, Yamane K, Sun ZW, LaCelle M, Zuber P, Nakano MM.





  
Title:
        The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.




  
PubMed:
        7868621


MedLine:
        




    
Source:
J Bacteriol. 1995 Mar;177(5):1409-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7868621









           
Homologs By Global Alignment 

           

       



     
Gene ID:154
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
280
                           PA1781_tr
                           translation of PA1781
                           816
                           99.50
                           99.75
                           0
                           4253.0
     

     
     
18268
                           gb|AAG05170.1|AE004603_10
                           (AE004603) assimilatory nitrite reductase large subunit [Pseudomonas aeruginosa PAO1]
                           816
                           99.50
                           99.75
                           0
                           4253.0
     

     
     
18269
                           gb|AAM41296.1|
                           (AE012305) nitrite reductase [NAD(P)H] [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           821
                           72.71
                           84.28
                           9
                           3158.0
     

     
     
18270
                           gb|AAP30018.1|
                           (AY163854) assimilatory nitrite reductase large subunit [Pseudomonas chlororaphis]
                           816
                           62.13
                           67.64
                           227
                           2720.0
     

     
     
18271
                           gb|AAM73544.1|AF283498_4
                           (AF283498) assimilatory nitrite reductase large subunit [Azospirillum brasilense]
                           832
                           58.41
                           72.83
                           27
                           2520.0
     

     






      

          
GC ORFID: 43156How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1781Glimmer Score: 

          
Start: 1247862Stop: 1245412

          
Length: 2451


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1781 translation of PA1781Homolog Bit Score 1642.0

          
Other Homologs:  t_PA1781 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGAAGATCAAGCTGGTAATGGTCGGCAACGGCATGGCCGGGGTCCGTACCCTGGAAGAACTGCTCAAG 
CTCAACTCGGACTTCTACGACATCACGGTGTTCGGCGCCGAGCCCCATCCCAACTACAACCGCATCCTGCTG 
TCCCCGGTACTGGCCGGCGAGCAGACCTTCGAGGAAATCGTCCTCAACGACCTCAACTGGTATGCGGAAAAC 
GGCATCAAGCTCCTCCTCGACCGCAAGGTGGTGCAGATCGACCGGGTCCGCCGCAGGGTGGTCGCCGCGGAC 
GGCAGCGAGGCCGAGTACGACCGCCTGCTGCTGGCCACCGGCTCGGTCCCCTTCATCCTGCCGATACCCGGC 
AATCGGCTGCAGGGCGTGATCGGCTACCGCGACATCGCCGACACCCAGGCCATGATCGACTGCGCCCGGACC 
CACAGCCACGCGGTGGTGATCGGCGGCGGCCTGCTCGGCCTGGAGGCGGCCAACGGCCTCAAGCAACGCGGC 
ATGGACGTGACCGTCGTGCACCTCTCCGACTGGCTGCTTGAACGCCAACTGGACCGCACCGCCGGCAAACTC 
CTGCAAGGCGCGCTGGAAGCCCGCGGCATCCGCTTCCGCCTGAACACCCAGACCCAGGAACTGATGGACAAC 
GGCAGCGGCCGGGTCTGTGCCGTGCAGTTCAACGACGGCGACGTGATTCCCGCCGACCTGGTGGTAATGGCC 
GCCGGTATCCGCCCCAACACCGAGCTGGCCGAAAGCGCAGGCATCCCCTGCAACCGCGGCATCCTGGTCAAC 
GACACCCTGCAGACCTACGACCCACGCATCTATGCGGTCGGCGAATGCGCCAACCACCGTGGCATCGCCTAC 
GGCCTGGTTGCGCCGCTGTTCGAGCAGGCCAAGGTCTGCGCCAACCACCTGGCTCATCTCGGCTACGCCCGC 
TACCAGGGCTCGGTGACCTCGACCAAGCTCAAGGTGACCGGTATCGACCTGTTCTCCGCCGGCGACTTCATC 
GGCGGCGAAGGCAGCGAGACCATCACCCTTTCCGACCCCATCGGCGGCGTCTACAAGAAACTGGTGATCAAG 
GACGATGTACTGGTCGGCGCCTGTCTCTACGGCGACACCGCCGACGGCGGCTGGTACTTCCGGCAGATCCGC 
GAGAACCACAACGTCGCGCAGATCCGCGACCACCTGATGTTCGGCGAGAACGCCCTCGGCGATGTCGGCCAC 
CAGGGCCAGAACAGCGCCGCGAGCATGCCCGACACGGCCGAAGTATGCGGCTGCAACGGCGTCTGCAAGGGC 
ACCATCGTCAAGGCGATACAAGAACACGGCCTGTTCAGCGTCGACGAAGTCAAGAAACACACCAAGGCCGCC 
AGCTCCTGCGGCTCCTGCGCGGGATTGGTGGAACAGATCCTGATCAGCACCGTCGGCGGCGCCGCCGACGTC 
AAGCCGAAGAGCGAGAAGGCCATCTGCGGCTGCAGCGACCTCAACCACGGGCAGATCCGCAAGGCCATTCGC 
GAACACCGGCTGACCAGTCTCGACGCCGTCATGCGCTTCATGGACTGGAAGACCCCGAACGGCTGTGCCACC 
TGCCGCCCGGCCCTGAACTATTACCTGATCTCGACCTGGCCGGGAGAAGCCAAGGACGATCCGCAGTCGCGC 
CTGATCAACGAACGCGCCCACGCCAACATCCAGAAGGACGGCACCTACTCGGTGGTACCGCGGATGTGGGGC 
GGCGTGACCAACGCCGCCGAGTTGCGGCGCATCGCCGACGTCGCCGACAAGTACCAGGTGCCGATGGTCAAG 
GTCACCGGTGGCCAGCGCATCGACCTGCTGGGGGTGAAGAAGGACGACCTGCCGGCCATCTGGAAAGACCTC 
GACATGCCCTCCGGACATGCCTACGGCAAATCCATCCGCACCGTGAAGACCTGCGTCGGCAGCGAGTTCTGC 
CGCTTCGGCACGCAAAACTCGACGCAGCTGGGCATCGATCTGGAACACGACCTGTTCAACATGTGGTCGCCG 
CACAAGGTCAAGCTGGCGGTCTCCGGCTGCCCGCGCAACTGCGCCGAGGCCGGGATCAAGGACGTCGGCATC 
ATCGGCGTGGACTCCGGCTGGGAGATGTACATCGGCGGCAACGGCGGGATCAAGACCGAGGTCGCGGAGTTC 
TTCGTCAAGCTCAAGACCGCCGAAGAAGTGCGCGAATACAACGGCGCCTTCCTCCAGCTCTACCGCGAGGAA 
GCCTTCTACCTGGAGCGCACGGTGCATTACCTGCAGCGGGTCGGCATGGAGCACATCCGCAAGGCGGTGGTC 
GAGGACGCGGAAAACCGCAAGGCGCTCAACGACCGCCTGCAATTCGCCCTGTCCTTCGAGCAGGACCCATGG 
AAGCAGCGCATCGAACAGCCGCAATTGAAGAAGGAGTTCGAACGGATTCCGCTCAAGCAACTGGAAAACGTC 
TGA