ViewGene
Locus Name:
GeneID:
Location:

GeneID:1536
TrivialName: PA14_11580
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11580
PAO1 Ortholog Locus:PA4041
Sequence Length:1170
Protein Length:389
Start: 1001095
Stop: 1002264
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07428.1|AE004821_1 (AE004821) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 385/389 (98%), Positives = 386/389 (99%)
Sequence:
ATGCGCATCCAGGAGGTCCGGGAACGCAGTATTCCCCTTTCCCGTTACGCCGGCGTGGCGGCTGCCACCGGT
GGTTTGAGCACCAGCCTGGTGGCGGTGCTCACCGATGCCGTGCGTGACGGTCGGCGTGTGGTCGGCTACGGC
CACGCCTCGGTAGGGCGCTACGCCCAGGGCGGGCTGATCCGCGAGCGTTTCGCCCCGCGCCTGCTGGGGGCT
CCGGCGGACAGCCTGGCGAGCGACGACGGCAGTGGCATCGATCCGTTGCGTGCCTGGGACCTGATGATGCGC
GACGAGAAGCCCGGCGGCCACGGCGAACGCTGCGTCGCGGTGGGCGCCCTGGACATGGCCCTGTGGGATGCC
GCGGCCAAGGCCGCCGGCCTGCCGCTGCATCGCTTCCTGGCGCGACGCCTGGGGCTGGCGGAAGACTATCCG
CAGCGCGTACCGGTCTACGCCGGCGGCGGCTACCCCTATCCGCACGACGACCTGGGACGCCTGGAAGAGGAA
CTGCGGCGCTTCGTCGATCTCGGCTACACCCACGCCAAGATCAAGATCGGCGCGGCCGGGCTGGACCAGGAC
CGGCGCCGTATCGACAGGGCGCTGCGGGTTCTCGGCGACGCTTCGCGGCTGGCGGTCGACGCGATGAACCGC
TACGACGCGGGGGGCGCGGTCGACGCAGCGAAAGCCCTGGCGCCGCTGGGGTTGTGGTGGTTCGAGGACATC
TGCGACCCCCACGATTTCCCTACCCAGGCCGAAGTGGCGGATATCTATCCGGGGCCCATCGGTGCCGGCGAG
GCCTTGTTCTCCCTGGCCGAAGCTCGCTTGCTGGACCTCTATGGCGGCTTGCGGCGGGAGCGTGACGTGCTG
GTCTTCGACCCCGTGCATTGCTATGGCCTGCCGGGCTACCTGGCGATACTCGACCACCTGTGCGCCCGGGGT
TGGCCGCGCGCGGCCTTCCGCCCGCACGCCGGCTACCTGTTCAGCCTGCACCTGGTGGCGGCCCTGCGCCTG
GGCTGGGCCGAGGCTACGCCGTCGGCTTTCCGGCCATTCGACGATTTCGCCGGTGCCCTGCCGTTTTCCTCG
GGACTGGCGGGGTTGCCACAATCGCCTGGTATCGGCTTCGAGCGCCAGGCGACACTTGCCGGGCTGTTCCAC
AAGCTTCTGGAGGCCTAG
Translation:
MRIQEVRERSIPLSRYAGVAAATGGLSTSLVAVLTDAVRDGRRVVGYGHASVGRYAQGGLIRERFAPRLLGA
PADSLASDDGSGIDPLRAWDLMMRDEKPGGHGERCVAVGALDMALWDAAAKAAGLPLHRFLARRLGLAEDYP
QRVPVYAGGGYPYPHDDLGRLEEELRRFVDLGYTHAKIKIGAAGLDQDRRRIDRALRVLGDASRLAVDAMNR
YDAGGAVDAAKALAPLGLWWFEDICDPHDFPTQAEVADIYPGPIGAGEALFSLAEARLLDLYGGLRRERDVL
VFDPVHCYGLPGYLAILDHLCARGWPRAAFRPHAGYLFSLHLVAALRLGWAEATPSAFRPFDDFAGALPFSS
GLAGLPQSPGIGFERQATLAGLFHKLLEA*
AnnotationID:1545GeneID:1536
AnnotatorUID: urbach
Modification Date/Time: 2004-10-05 17:05:33
Gene Name:
Confidence Code:3
GeneProduct:putative racemase
Cell Localization:()
Synonyms:PA4041
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4948
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
gb|AAC15504.1| (AY143338) mandelate racemase [Pseudomonas putida]
Length = 359
Score = 63.5 bits (153), Expect = 2e-09
Identities = 69/272 (25%), Positives = 97/272 (35%), Gaps = 34/272 (12%)






 
Structural Features:
gnl|CDD|14081 COG4948, COG4948, L-alanine-DL-glutamate epimerase and related
enzymes of enolase superfamily [Cell envelope biogenesis,
outer membrane / General function prediction only]
Length = 372
Score =  174 bits (443), Expect = 2e-44
Identities = 99/396 (25%), Positives = 150/396 (37%), Gaps = 31/396 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3075




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20221




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34626




  
Author/Investigator(s):
        Tsou AY, Ransom SC, Gerlt JA, Buechter DD, Babbitt PC, Kenyon GL.





  
Title:
        Mandelate pathway of Pseudomonas putida: sequence relationships involving mandelate racemase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase and expression of benzoylformate decarboxylase in Escherichia coli.




  
PubMed:
        2271624


MedLine:
        




    
Source:
Biochemistry. 1990 Oct 23;29(42):9856-62.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=91104894&dopt=Citation









           
Homologs By Global Alignment 

           

       



     
Gene ID:1536
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2726
                           PA4041_tr
                           translation of PA4041
                           389
                           98.97
                           99.22
                           0
                           2016.0
     

     
     
85448
                           gb|AAG07428.1|AE004821_1
                           (AE004821) hypothetical protein PA4041 [Pseudomonas aeruginosa PAO1]
                           389
                           98.97
                           99.22
                           0
                           2016.0
     

     
     
85450
                           dbj|BAC51995.1|
                           (AP005959) bll6730 [Bradyrhizobium japonicum USDA 110]
                           397
                           45.34
                           60.70
                           16
                           824.0
     

     
     
85449
                           gb|AAO57519.1|
                           (AE016870) mandelate racemase/muconate lactonizing enzyme family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           392
                           44.13
                           60.96
                           6
                           832.0
     

     
     
85451
                           emb|CAE33307.1|
                           (BX640445) putative mandelate racemase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           405
                           42.22
                           55.55
                           29
                           764.0
     

     






      

          
GC ORFID: 44538How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4041Glimmer Score: 

          
Start: 3952842Stop: 3951673

          
Length: 1170


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4041 translation of PA4041Homolog Bit Score 781.0

          
Other Homologs:  t_PA4041 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCATCCAGGAGGTCCGGGAACGCAGTATTCCCCTTTCCCGTTACGCCGGCGTGGCGGCTGCCACCGGT 
GGTTTGAGCACCAGCCTGGTGGCGGTGCTCACCGATGCCGTGCGTGACGGTCGGCGTGTGGTCGGCTACGGC 
CACGCCTCGGTAGGGCGCTACGCCCAGGGCGGGCTGATCCGCGAGCGTTTCGCCCCGCGCCTGCTGGGGGCT 
CCGGCGGACAGCCTGGCGAGCGACGACGGCAGTGGCATCGATCCGTTGCGTGCCTGGGACCTGATGATGCGC 
GACGAGAAGCCCGGCGGCCACGGCGAACGCTGCGTCGCGGTGGGCGCCCTGGACATGGCCCTGTGGGATGCC 
GCGGCCAAGGCCGCCGGCCTGCCGCTGCATCGCTTCCTGGCGCGACGCCTGGGGCTGGCGGAAGACTATCCG 
CAGCGCGTACCGGTCTACGCCGGCGGCGGCTACCCCTATCCGCACGACGACCTGGGACGCCTGGAAGAGGAA 
CTGCGGCGCTTCGTCGATCTCGGCTACACCCACGCCAAGATCAAGATCGGCGCGGCCGGGCTGGACCAGGAC 
CGGCGCCGTATCGACAGGGCGCTGCGGGTTCTCGGCGACGCTTCGCGGCTGGCGGTCGACGCGATGAACCGC 
TACGACGCGGGGGGCGCGGTCGACGCAGCGAAAGCCCTGGCGCCGCTGGGGTTGTGGTGGTTCGAGGACATC 
TGCGACCCCCACGATTTCCCTACCCAGGCCGAAGTGGCGGATATCTATCCGGGGCCCATCGGTGCCGGCGAG 
GCCTTGTTCTCCCTGGCCGAAGCTCGCTTGCTGGACCTCTATGGCGGCTTGCGGCGGGAGCGTGACGTGCTG 
GTCTTCGACCCCGTGCATTGCTATGGCCTGCCGGGCTACCTGGCGATACTCGACCACCTGTGCGCCCGGGGT 
TGGCCGCGCGCGGCCTTCCGCCCGCACGCCGGCTACCTGTTCAGCCTGCACCTGGTGGCGGCCCTGCGCCTG 
GGCTGGGCCGAGGCTACGCCGTCGGCTTTCCGGCCATTCGACGATTTCGCCGGTGCCCTGCCGTTTTCCTCG 
GGACTGGCGGGGTTGCCACAATCGCCTGGTATCGGCTTCGAGCGCCAGGCGACACTTGCCGGGCTGTTCCAC 
AAGCTTCTGGAGGCCTAG