ViewGene
Locus Name:
GeneID:
Location:

GeneID:1530
TrivialName: PA14_06510
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_06510
PAO1 Ortholog Locus:PA0501
Sequence Length:1206
Protein Length:401
Start: 570781
Stop: 571986
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03890.1|AE004487_4 (AE004487) 8-amino-7-oxononanoate synthase [Pseudomonas aeruginosa PAO1]
Identities = 400/401 (99%), Positives = 400/401 (99%)
Sequence:
ATGTCCTTCGATCTCGCTTCCCGCCTCGCCAGCCGGCGCGCGGAAGACCTCTACCGCCAGCGGCCGCTGCTG
GAGTCGGCCCAGGGCCCGGACGTCGTGGTCGACGGCCAGCCGCTGCTGGCCTTCTGTTCCAACGACTACCTC
GGCCTGGCCAATCATCCCGAAGTGATCGCCGCGTTACGCGCCGGCGCCGAGCGCTGGGGCGTGGGCGGCGGC
GCCTCGCACCTGGTGGTCGGCCACAGCGGTCCGCACCACGAACTGGAGCTGGCCCTCGCCGAATTCACCGGG
CGGCCGCGCGCGCTGCTGTTCTCCACCGGCTACATGGCCAACCTTGGCGCGGTGACCGCGCTGGTCGGCAAG
GGCGACACGGTGCTGGAGGACCGCCTCAACCACGCTTCGCTGCTGGATGCCGGATTGCTCTCCGGCGCGCGT
TTCTCGCGCTACCTGCACAACGACCCGGCAAGCCTCGCCGCGCGCCTGGACAAGGCCGAGGGCAATACCCTG
GTGGTCACCGATGGGGTCTTCAGCATGGACGGCAATCTCGCCGACCTGCCGGCCCTGGCCGCCGTCGCGCAG
GCCCGCGGCGCCTGGCTGATGGTCGACGACGCGCATGGCTTCGGCCCGCTGGGCGCCAGCGGCGGCGGGATC
GTCGAACACTTCGGCCTCGGCCAGGAGCAGGTGCCGGTGCTGATCGGCACCCTCGGCAAGGGTTTCGGTACC
GCCGGCGCCTTCGTAGCGGGCAGCGAGGAACTGATCGAGACCCTGATCCAGTACGCCCGGCCCTACATCTAC
ACCACCAGCCAGCCGCCAGCGGTGGCCTGCGCCACCCTGAAGAGCCTGGAGCTGCTGCGCCGCGAAAGCTGG
CGGCGGCAACACCTGGCGGCGCTGATCGCCCGTTTCCGCCATGGCGCCGAGGCGCTTGGCCTGACCCTGATG
GACAGCTTCACGCCGATCCAGCCGATCCTGGTCGGCGGCAGCCGCCAGGCCGTGGCCCTGGCCGGCATGCTT
CGAGCCCGCGGCATCATGGTCGGCGCGATCCGCCCGCCAACCGTGCCGGCCAACAGCGCGCGGCTGCGCGTC
ACGCTGTCCGCCGCGCACAGCGAAGCGCAGGTCGACCGCTTGCTCGAAGCCCTCGGCGAAAGCTGGCGACAG
CTGTCGTCTAGCCTTCTGGCAGAGATCGAAGCCGAGGAGGGAGACGATGCGTGA
Translation:
MSFDLASRLASRRAEDLYRQRPLLESAQGPDVVVDGQPLLAFCSNDYLGLANHPEVIAALRAGAERWGVGGG
ASHLVVGHSGPHHELELALAEFTGRPRALLFSTGYMANLGAVTALVGKGDTVLEDRLNHASLLDAGLLSGAR
FSRYLHNDPASLAARLDKAEGNTLVVTDGVFSMDGNLADLPALAAVAQARGAWLMVDDAHGFGPLGASGGGI
VEHFGLGQEQVPVLIGTLGKGFGTAGAFVAGSEELIETLIQYARPYIYTTSQPPAVACATLKSLELLRRESW
RRQHLAALIARFRHGAEALGLTLMDSFTPIQPILVGGSRQAVALAGMLRARGIMVGAIRPPTVPANSARLRV
TLSAAHSEAQVDRLLEALGESWRQLSSSLLAEIEAEEGDDA*
AnnotationID:1539GeneID:1530
AnnotatorUID: diggins
Modification Date/Time: 2005-03-28 04:04:05
Gene Name:bioF
Confidence Code:2
GeneProduct:8-amino-7-oxononanoate synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0156
Secondary Functional Category(ies):
EC Number:2.3.1.47
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65994.1|AE016228_7 (AE016775) 8-amino-7-oxononanoate synthase [Pseudomonas putida
           KT2440]
          Length = 390

 Score =  628 bits (1620), Expect = e-179
 Identities = 318/388 (81%), Positives = 349/388 (89%)

>gb|AAO54039.1| (AE016857) 8-amino-7-oxononanoate synthase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 396

 Score =  615 bits (1586), Expect = e-175
 Identities = 308/388 (79%), Positives = 344/388 (88%)

>emb|CAD86211.1| (BX321864) Aminotransferases class-I [Nitrosomonas europaea ATCC
           19718]
          Length = 387

 Score =  390 bits (1002), Expect = e-108
 Identities = 214/381 (56%), Positives = 255/381 (66%), Gaps = 3/381 (0%)






 
Structural Features:
COG0156, BioF, 7-keto-8-aminopelargonate synthetase and related enzymes 
[Coenzyme metabolism]
CD-Length = 388 residues,  99.0% aligned
Score =  425 bits (1094), Expect = 4e-120
Identities = 187/384 (48%), Positives = 250/384 (65%), Gaps = 4/384 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3063




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20209




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33812




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1530_cdd.html


    		




 
URL:

















    ReferenceID:35325




  
Author/Investigator(s):
        Otsuka AJ, Buoncristiani MR, Howard PK, Flamm J, Johnson C, Yamamoto R, Uchida K, Cook C, Ruppert J, Matsuzaki J.





  
Title:
         
The Escherichia coli biotin biosynthetic enzyme sequences predicted from the nucleotide sequence of the bio operon.




  
PubMed:
        3058702


MedLine:
        




    
Source:
J Biol Chem. 1988 Dec 25;263(36):19577-85.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3058702

















    ReferenceID:35329




  
Author/Investigator(s):
        Kiyasu T, Nagahashi Y, Hoshino T.





  
Title:
        Cloning and characterization of biotin biosynthetic genes of Kurthia sp.




  
PubMed:
        11255013


MedLine:
        




    
Source:
Gene. 2001 Mar 7;265(1-2):103-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11255013

















    ReferenceID:35330




  
Author/Investigator(s):
        Wu CH, Bao YY, Shao CP, Shiuan D.





  
Title:
        Molecular cloning and nucleotide sequencing of bioF (7-keto-8-amino pelargonic acid synthetase), bioC and bioD (dethiobiotin synthetase) genes of Erwinia herbicola.




  
PubMed:
        9063571


MedLine:
        




    
Source:
Biochem Mol Biol Int. 1997 Feb;41(2):311-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9063571

















    ReferenceID:35331




  
Author/Investigator(s):
        Sakurai N, Akatsuka H, Kawai E, Imai Y, Komatsubara S.





  
Title:
        Complete sequence and organization of the Serratia marcescens biotin operon.




  
PubMed:
        8969526


MedLine:
        




    
Source:
Microbiology. 1996 Nov;142 ( Pt 11):3295-303.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8969526

















    ReferenceID:35332




  
Author/Investigator(s):
        Bower S, Perkins JB, Yocum RR, Howitt CL, Rahaim P, Pero J.





  
Title:
        Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.




  
PubMed:
        8763940


MedLine:
        




    
Source:
J Bacteriol. 1996 Jul;178(14):4122-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8763940









           
Homologs By Global Alignment 

           

       



     
Gene ID:1530
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2720
                           PA0501_tr
                           translation of PA0501
                           401
                           99.75
                           99.75
                           0
                           2019.0
     

     
     
85391
                           gb|AAG03890.1|AE004487_4
                           (AE004487) 8-amino-7-oxononanoate synthase [Pseudomonas aeruginosa PAO1]
                           401
                           99.75
                           99.75
                           0
                           2019.0
     

     
     
85392
                           gb|AAN65994.1|AE016228_7
                           (AE016775) 8-amino-7-oxononanoate synthase [Pseudomonas putida KT2440]
                           401
                           80.04
                           87.28
                           11
                           1616.0
     

     
     
85393
                           gb|AAO54039.1|
                           (AE016857) 8-amino-7-oxononanoate synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           402
                           77.11
                           86.31
                           7
                           1583.0
     

     
     
85394
                           emb|CAD86211.1|
                           (BX321864) Aminotransferases class-I [Nitrosomonas europaea ATCC 19718]
                           404
                           53.21
                           63.86
                           20
                           1005.0
     

     






      

          
GC ORFID: 44532How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0501Glimmer Score: 

          
Start: 6372123Stop: 6373328

          
Length: 1206


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0501 translation of PA0501Homolog Bit Score 782.0

          
Other Homologs:  t_PA0501 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCTTCGATCTCGCTTCCCGCCTCGCCAGCCGGCGCGCGGAAGACCTCTACCGCCAGCGGCCGCTGCTG 
GAGTCGGCCCAGGGCCCGGACGTCGTGGTCGACGGCCAGCCGCTGCTGGCCTTCTGTTCCAACGACTACCTC 
GGCCTGGCCAATCATCCCGAAGTGATCGCCGCGTTACGCGCCGGCGCCGAGCGCTGGGGCGTGGGCGGCGGC 
GCCTCGCACCTGGTGGTCGGCCACAGCGGTCCGCACCACGAACTGGAGCTGGCCCTCGCCGAATTCACCGGG 
CGGCCGCGCGCGCTGCTGTTCTCCACCGGCTACATGGCCAACCTTGGCGCGGTGACCGCGCTGGTCGGCAAG 
GGCGACACGGTGCTGGAGGACCGCCTCAACCACGCTTCGCTGCTGGATGCCGGATTGCTCTCCGGCGCGCGT 
TTCTCGCGCTACCTGCACAACGACCCGGCAAGCCTCGCCGCGCGCCTGGACAAGGCCGAGGGCAATACCCTG 
GTGGTCACCGATGGGGTCTTCAGCATGGACGGCAATCTCGCCGACCTGCCGGCCCTGGCCGCCGTCGCGCAG 
GCCCGCGGCGCCTGGCTGATGGTCGACGACGCGCATGGCTTCGGCCCGCTGGGCGCCAGCGGCGGCGGGATC 
GTCGAACACTTCGGCCTCGGCCAGGAGCAGGTGCCGGTGCTGATCGGCACCCTCGGCAAGGGTTTCGGTACC 
GCCGGCGCCTTCGTAGCGGGCAGCGAGGAACTGATCGAGACCCTGATCCAGTACGCCCGGCCCTACATCTAC 
ACCACCAGCCAGCCGCCAGCGGTGGCCTGCGCCACCCTGAAGAGCCTGGAGCTGCTGCGCCGCGAAAGCTGG 
CGGCGGCAACACCTGGCGGCGCTGATCGCCCGTTTCCGCCATGGCGCCGAGGCGCTTGGCCTGACCCTGATG 
GACAGCTTCACGCCGATCCAGCCGATCCTGGTCGGCGGCAGCCGCCAGGCCGTGGCCCTGGCCGGCATGCTT 
CGAGCCCGCGGCATCATGGTCGGCGCGATCCGCCCGCCAACCGTGCCGGCCAACAGCGCGCGGCTGCGCGTC 
ACGCTGTCCGCCGCGCACAGCGAAGCGCAGGTCGACCGCTTGCTCGAAGCCCTCGGCGAAAGCTGGCGACAG 
CTGTCGTCTAGCCTTCTGGCAGAGATCGAAGCCGAGGAGGGAGACGATGCGTGA