ViewGene
Locus Name:
GeneID:
Location:

GeneID:1524
TrivialName: PA14_44000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_44000
PAO1 Ortholog Locus:PA1586
Sequence Length:1230
Protein Length:409
Start: 3916391
Stop: 3915162
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04975.1|AE004586_13 (AE004586) dihydrolipoamide succinyltransferase (E2 subunit) [Pseudomonas aeruginosa PAO1]
Identities = 408/409 (99%), Positives = 408/409 (99%)
Sequence:
ATGGCTATCGAAATCAAAGCCCCAACCTTCCCGGAATCGGTCGCCGACGGCACCGTCGCCACCTGGCACAAG
AAGCCGGGCGAAGCGGTCAAGCGCGACGAACTGATCGTCGACATCGAGACCGACAAGGTGGTCATCGAGGTT
CTCGCCGAGGCTGACGGCGTGCTGGCCGAGATCATCAAGAACGAAGGCGACACCGTTCTGAGCAACGAGCTG
CTCGGCAAGCTGAACGAAGGCGGCGCCGCCGCTCCGGCCGCTCCGGCTGCCGCTGCGCCCGCCGCCGCTCCG
GCTGCCCAGGCCGCCGCTCCGGCCGCCGCTGGCGGCGACGACGCCATCCTGTCCCCGGCCGCGCGCAAGCTG
GCCGAGGAGGCCGGCATCGATCCGAACAGCATCGCCGGCACCGGCAAGGGCGGTCGCGTGACCAAGGAAGAC
GTGGTCGCTGCGGTCGAAGCGAAGAAGAACGCCCCGGCCGCCCTGGCCAAGCCTGCCGCCCCGGCTGCCGAG
GCGCCGATCTTCGCCGCCGGCGACCGTGTCGAGAAGCGCGTGCCGATGACCCGCCTGCGGGCCAAGGTCGCC
GAGCGCCTGGTCGAGGCGCAGTCCGCCATGGCCATGCTGACCACCTTCAACGAGGTCAACATGAAGCCGATC
ATGGACCTGCGTTCGAAGTACAAGGACCTGTTCGAGAAGAAGCACAACGGCGTGCGCCTGGGCTTCATGTCC
TTCTTCGTCAAGGCCGCCACCGAGGCGCTGAAGCGCTTCCCGGGGGTCAACGCTTCCATCGACGGCAACGAC
ATCGTCTACCACGGCTACCAGGACATCGGCGTTGCCGTTTCCAGCGACCGCGGCCTGGTGGTTCCGGTACTG
CGTAACGCCGAGTTCATGAGCCTGGCCGAGATCGAGGGCGGCATCGCCAACTTCGGCAAGAAGGCGAAGGAA
GGCAAGCTGACCATCGAAGACATGACCGGCGGCACCTTCACCATCTCCAACGGTGGCGTGTTCGGTTCCCTG
CTGTCGACCCCGATCGTCAACCCGCCGCAGACCGCCATCCTCGGCATGCACAAGATCCAGGAGCGCCCGATG
GCCGTGAACGGCCAGGTGGTGATCCTGCCGATGATGTATCTGGCGCTGTCCTACGACCACCGTCTGATCGAC
GGCAAGGAAGCGGTGAGCTTCCTGGTGGCGATCAAGGACCTGCTGGAAGATCCGGCTCGCCTGCTGCTGGAC
GTCTGA
Translation:
MAIEIKAPTFPESVADGTVATWHKKPGEAVKRDELIVDIETDKVVIEVLAEADGVLAEIIKNEGDTVLSNEL
LGKLNEGGAAAPAAPAAAAPAAAPAAQAAAPAAAGGDDAILSPAARKLAEEAGIDPNSIAGTGKGGRVTKED
VVAAVEAKKNAPAALAKPAAPAAEAPIFAAGDRVEKRVPMTRLRAKVAERLVEAQSAMAMLTTFNEVNMKPI
MDLRSKYKDLFEKKHNGVRLGFMSFFVKAATEALKRFPGVNASIDGNDIVYHGYQDIGVAVSSDRGLVVPVL
RNAEFMSLAEIEGGIANFGKKAKEGKLTIEDMTGGTFTISNGGVFGSLLSTPIVNPPQTAILGMHKIQERPM
AVNGQVVILPMMYLALSYDHRLIDGKEAVSFLVAIKDLLEDPARLLLDV*
AnnotationID:1533GeneID:1524
AnnotatorUID: diggins
Modification Date/Time: 2005-04-28 06:06:56
Gene Name:sucB
Confidence Code:2
GeneProduct:dihydrolipoamide succinyltransferase (E2 subunit)
Cell Localization:()
Synonyms:kgdB
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase
Functional Category Confidence Code:5
COGs:COG0508,pfam00198
Secondary Functional Category(ies):
EC Number:2.3.1.61
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAA36678.1| (X52432) succinyltransferase [Azotobacter vinelandii]
          Length = 399

 Score =  671 bits (1732), Expect = 0.0
 Identities = 355/409 (86%), Positives = 372/409 (90%), Gaps = 10/409 (2%)

>gb|AAN69769.1|AE016613_4 (AE016789) 2-oxoglutarate dehydrogenase, dihydrolipoamide
           succinyltransferase [Pseudomonas putida KT2440]
          Length = 407

 Score =  638 bits (1645), Expect = 0.0
 Identities = 337/411 (81%), Positives = 365/411 (88%), Gaps = 6/411 (1%)

>gb|AAO55716.1| (AE016863) 2-oxoglutarate dehydrogenase, E2 component,
           dihydrolipoamide succinyltransferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 406

 Score =  614 bits (1584), Expect = e-175
 Identities = 316/409 (77%), Positives = 357/409 (87%), Gaps = 3/409 (0%)








 
Structural Features:
COG0508, AceF, Pyruvate/2-oxoglutarate dehydrogenase complex, 
dihydrolipoamide acyltransferase (E2) component, and related enzymes 
[Energy production and conversion]
CD-Length = 404 residues, 100.0% aligned
Score =  410 bits (1055), Expect = 1e-115
Identities = 225/412 (54%), Positives = 291/412 (70%), Gaps = 12/412 (2%)

pfam00198, 2-oxoacid_dh, 2-oxoacid dehydrogenases acyltransferase 
(catalytic domain).
CD-Length = 232 residues,  98.7% aligned
Score =  297 bits (763), Expect = 1e-81
Identities = 127/231 (54%), Positives = 169/231 (73%), Gaps = 4/231 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:3051




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20197




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36509




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1524_cdd.html


    		




 
URL:

















    ReferenceID:36577




  
Author/Investigator(s):
        Spencer ME, Darlison MG, Stephens PE, Duckenfield IK, Guest JR.





  
Title:
        Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase.




  
PubMed:
        6376124


MedLine:
        




    
Source:
Eur J Biochem. 1984 Jun 1;141(2):361-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=6376124









           
Homologs By Global Alignment 

           

       



     
Gene ID:1524
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2713
                           PA1586_tr
                           translation of PA1586
                           409
                           99.75
                           99.75
                           0
                           2024.0
     

     
     
85246
                           gb|AAG04975.1|AE004586_13
                           (AE004586) dihydrolipoamide succinyltransferase (E2 subunit) [Pseudomonas aeruginosa PAO1]
                           409
                           99.75
                           99.75
                           0
                           2024.0
     

     
     
85247
                           emb|CAA36678.1|
                           (X52432) succinyltransferase [Azotobacter vinelandii]
                           409
                           86.79
                           90.95
                           10
                           1741.0
     

     
     
85248
                           gb|AAN69769.1|AE016613_4
                           (AE016789) 2-oxoglutarate dehydrogenase, dihydrolipoamide succinyltransferase [Pseudomonas putida KT2440]
                           411
                           81.99
                           88.80
                           6
                           1652.0
     

     
     
85249
                           gb|AAC23517.1|
                           (AF068740) dihydrolipoamide succinyltransferase; E2 [Pseudomonas putida]
                           411
                           81.26
                           88.32
                           6
                           1642.0
     

     






      

          
GC ORFID: 44526How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1586Glimmer Score: 

          
Start: 1037518Stop: 1038747

          
Length: 1230


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1586 translation of PA1586Homolog Bit Score 784.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene998 (ORF: BLASTX 616.0), t_PA1586 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCTATCGAAATCAAAGCCCCAACCTTCCCGGAATCGGTCGCCGACGGCACCGTCGCCACCTGGCACAAG 
AAGCCGGGCGAAGCGGTCAAGCGCGACGAACTGATCGTCGACATCGAGACCGACAAGGTGGTCATCGAGGTT 
CTCGCCGAGGCTGACGGCGTGCTGGCCGAGATCATCAAGAACGAAGGCGACACCGTTCTGAGCAACGAGCTG 
CTCGGCAAGCTGAACGAAGGCGGCGCCGCCGCTCCGGCCGCTCCGGCTGCCGCTGCGCCCGCCGCCGCTCCG 
GCTGCCCAGGCCGCCGCTCCGGCCGCCGCTGGCGGCGACGACGCCATCCTGTCCCCGGCCGCGCGCAAGCTG 
GCCGAGGAGGCCGGCATCGATCCGAACAGCATCGCCGGCACCGGCAAGGGCGGTCGCGTGACCAAGGAAGAC 
GTGGTCGCTGCGGTCGAAGCGAAGAAGAACGCCCCGGCCGCCCTGGCCAAGCCTGCCGCCCCGGCTGCCGAG 
GCGCCGATCTTCGCCGCCGGCGACCGTGTCGAGAAGCGCGTGCCGATGACCCGCCTGCGGGCCAAGGTCGCC 
GAGCGCCTGGTCGAGGCGCAGTCCGCCATGGCCATGCTGACCACCTTCAACGAGGTCAACATGAAGCCGATC 
ATGGACCTGCGTTCGAAGTACAAGGACCTGTTCGAGAAGAAGCACAACGGCGTGCGCCTGGGCTTCATGTCC 
TTCTTCGTCAAGGCCGCCACCGAGGCGCTGAAGCGCTTCCCGGGGGTCAACGCTTCCATCGACGGCAACGAC 
ATCGTCTACCACGGCTACCAGGACATCGGCGTTGCCGTTTCCAGCGACCGCGGCCTGGTGGTTCCGGTACTG 
CGTAACGCCGAGTTCATGAGCCTGGCCGAGATCGAGGGCGGCATCGCCAACTTCGGCAAGAAGGCGAAGGAA 
GGCAAGCTGACCATCGAAGACATGACCGGCGGCACCTTCACCATCTCCAACGGTGGCGTGTTCGGTTCCCTG 
CTGTCGACCCCGATCGTCAACCCGCCGCAGACCGCCATCCTCGGCATGCACAAGATCCAGGAGCGCCCGATG 
GCCGTGAACGGCCAGGTGGTGATCCTGCCGATGATGTATCTGGCGCTGTCCTACGACCACCGTCTGATCGAC 
GGCAAGGAAGCGGTGAGCTTCCTGGTGGCGATCAAGGACCTGCTGGAAGATCCGGCTCGCCTGCTGCTGGAC 
GTCTGA