ViewGene
Locus Name:
GeneID:
Location:

GeneID:1518
TrivialName: PA14_38780
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38780
PAO1 Ortholog Locus:PA1989
Sequence Length:1146
Protein Length:381
Start: 3460278
Stop: 3459133
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05377.1|AE004625_7 (AE004625) pyrroloquinoline quinone biosynthesis protein E [Pseudomonas aeruginosa PAO1]
Identities = 381/381 (100%), Positives = 381/381 (100%)
Sequence:
ATGCGCAATTCTGGATCGAGCTGCAGTGAAAGCGTGGGGCCGCCGCTCTGGCTGCTGGCCGAGCTGACCTAT
CGCTGTCCGTTGCAGTGCCCGTACTGCTCGAACCCGCTGGAATTCGCCCGCGAGGGCGCCGAGCTGAGCACC
GCGGAATGGATCGAGGTGTTCCGCCAGGCTCGCGAGCTGGGCGCCGCACAGCTCGGTTTCTCCGGCGGCGAG
CCGCTGCTGCGCCAGGACCTCGCCGAACTGATCGAGGCAGGGCGCGGCCTGGGCTTCTACACCAACCTGATC
ACCTCCGGCATCGGCCTCGACGAGGCACGCCTGGCGCGCTTCGCCGAGGCCGGGCTGGACCACGTGCAGATC
AGCTTCCAGGCCGCCGACGAAGAAGTGAACAACCTGCTCGCCGGCTCACGCAAGGCCTTCGCCCAGAAACTG
GCGATGGCCCGCGCGGTGAAGGCGCACGGCTATCCGATGGTGCTCAACTTCGTCACCCACCGGCACAACATC
GACAACATCGAGCGGATCATCCAGCTGTGCATCGAGCTGGAAGCCGATTACGTCGAACTCGCCACTTGCCAG
TTCTACGGCTGGGCCGCGCTGAACCGTGCCGGGCTGCTGCCGACCCGCGCCCAACTGGAGCGCGCCGAGCGG
ATCACCGCCGAATACCGCCAACGGCTGGCCGCCGAAGGCAACCCGTGCAAGCTGATCTTCGTCACCCCCGAC
TACTACGAGGAACGGCCGAAGGCCTGCATGGGCGGCTGGGCCAGCGTATTCCTCGACATCACCCCCGACGGC
ACCGCGCTGCCGTGTCACAGCGCGCGGCAATTGCCGGTGCAGTTTCCCAACGTGCGCGAGCACAGCCTGCGC
CACATCTGGTACGAATCGTTCGGCTTCAACCGTTACCGCGGCGACGCCTGGATGCCCGAACCCTGCCGTTCC
TGCGAGGAGAAGGAGAGGGACCACGGCGGCTGCCGCTGCCAGGCGTTCCTCCTCACCGGCGATGCCGACGCC
ACCGACCCGGTCTGCGCCAAGTCGGCACGGCATGACCTGATCCTCGCGGCCCGTCGCCAGGCCGAGGAGGCG
CCGCTTGGCCTGGACGCGCTGACCTGGCGCAACCAGCGCGCGTCACGCCTGATCTGCAAGGCCTGA
Translation:
MRNSGSSCSESVGPPLWLLAELTYRCPLQCPYCSNPLEFAREGAELSTAEWIEVFRQARELGAAQLGFSGGE
PLLRQDLAELIEAGRGLGFYTNLITSGIGLDEARLARFAEAGLDHVQISFQAADEEVNNLLAGSRKAFAQKL
AMARAVKAHGYPMVLNFVTHRHNIDNIERIIQLCIELEADYVELATCQFYGWAALNRAGLLPTRAQLERAER
ITAEYRQRLAAEGNPCKLIFVTPDYYEERPKACMGGWASVFLDITPDGTALPCHSARQLPVQFPNVREHSLR
HIWYESFGFNRYRGDAWMPEPCRSCEEKERDHGGCRCQAFLLTGDADATDPVCAKSARHDLILAARRQAEEA
PLGLDALTWRNQRASRLICKA*
AnnotationID:1527GeneID:1518
AnnotatorUID: diggins
Modification Date/Time: 2005-03-02 17:05:00
Gene Name:pqqE
Confidence Code:2
GeneProduct:pyrroloquinoline quinone biosynthesis protein E
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:coenzyme PQQ synthesis protein E
Functional Category Confidence Code:5
COGs:COG0535
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66007.1|AE016229_8 (AE016775) coenzyme PQQ synthesis protein E [Pseudomonas putida
           KT2440]
          Length = 376

 Score =  611 bits (1576), Expect = e-174
 Identities = 287/369 (77%), Positives = 322/369 (87%)

>emb|CAA41583.1| (X58778) pqqE [Klebsiella pneumoniae]
          Length = 380

 Score =  563 bits (1451), Expect = e-160
 Identities = 265/373 (71%), Positives = 309/373 (82%)

>gb|AAG42542.1| (AY013584) coenzyme PQQ synthesis protein E [Sinorhizobium
           meliloti]
          Length = 375

 Score =  353 bits (906), Expect = 1e-96
 Identities = 174/336 (51%), Positives = 220/336 (65%), Gaps = 4/336 (1%)








 
Structural Features:
COG0535, COG0535, Predicted Fe-S oxidoreductases
CD-Length = 347 residues,  94.2% aligned
Score =  185 bits (471), Expect = 5e-48
Identities = 108/336 (32%), Positives = 146/336 (43%), Gaps = 14/336 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:3039




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:20185




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32739




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:1518_cdd.html


    		




 
URL:

















    ReferenceID:34372




  
Author/Investigator(s):
        Toyama H, Chistoserdova L, Lidstrom ME.





  
Title:
        Sequence analysis of pqq genes required for biosynthesis of pyrroloquinoline quinone in Methylobacterium extorquens AM1 and the purification of a biosynthetic intermediate.




  
PubMed:
        9043136


MedLine:
        




    
Source:
Microbiology. 1997 Feb;143 ( Pt 2):595-602.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9043136

















    ReferenceID:34375




  
Author/Investigator(s):
        Meulenberg JJ, Sellink E, Riegman NH, Postma PW.





  
Title:
        Nucleotide sequence and structure of the Klebsiella pneumoniae pqq operon.




  
PubMed:
        1313537


MedLine:
        




    
Source:
Mol Gen Genet. 1992 Mar;232(2):284-94.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1313537









           
Homologs By Global Alignment 

           

       



     
Gene ID:1518
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
2706
                           PA1989_tr
                           translation of PA1989
                           381
                           99.99
                           99.99
                           0
                           2026.0
     

     
     
85141
                           gb|AAG05377.1|AE004625_7
                           (AE004625) pyrroloquinoline quinone biosynthesis protein E [Pseudomonas aeruginosa PAO1]
                           381
                           99.99
                           99.99
                           0
                           2026.0
     

     
     
85142
                           gb|AAN66007.1|AE016229_8
                           (AE016775) coenzyme PQQ synthesis protein E [Pseudomonas putida KT2440]
                           381
                           75.32
                           84.77
                           5
                           1571.0
     

     
     
85143
                           gb|AAO54052.1|
                           (AE016857) coenzyme PQQ synthesis protein E [Pseudomonas syringae pv. tomato str. DC3000]
                           389
                           69.40
                           82.51
                           8
                           1507.0
     

     
     
85145
                           gb|AAA24822.1|
                           (M94448) gluconic acid [Pantoea agglomerans]
                           381
                           69.02
                           79.26
                           4
                           1431.0
     

     






      

          
GC ORFID: 44520How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1989Glimmer Score: 

          
Start: 1493633Stop: 1494778

          
Length: 1146


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1989 translation of PA1989Homolog Bit Score 785.0

          
Other Homologs:  t_PA1989 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCAATTCTGGATCGAGCTGCAGTGAAAGCGTGGGGCCGCCGCTCTGGCTGCTGGCCGAGCTGACCTAT 
CGCTGTCCGTTGCAGTGCCCGTACTGCTCGAACCCGCTGGAATTCGCCCGCGAGGGCGCCGAGCTGAGCACC 
GCGGAATGGATCGAGGTGTTCCGCCAGGCTCGCGAGCTGGGCGCCGCACAGCTCGGTTTCTCCGGCGGCGAG 
CCGCTGCTGCGCCAGGACCTCGCCGAACTGATCGAGGCAGGGCGCGGCCTGGGCTTCTACACCAACCTGATC 
ACCTCCGGCATCGGCCTCGACGAGGCACGCCTGGCGCGCTTCGCCGAGGCCGGGCTGGACCACGTGCAGATC 
AGCTTCCAGGCCGCCGACGAAGAAGTGAACAACCTGCTCGCCGGCTCACGCAAGGCCTTCGCCCAGAAACTG 
GCGATGGCCCGCGCGGTGAAGGCGCACGGCTATCCGATGGTGCTCAACTTCGTCACCCACCGGCACAACATC 
GACAACATCGAGCGGATCATCCAGCTGTGCATCGAGCTGGAAGCCGATTACGTCGAACTCGCCACTTGCCAG 
TTCTACGGCTGGGCCGCGCTGAACCGTGCCGGGCTGCTGCCGACCCGCGCCCAACTGGAGCGCGCCGAGCGG 
ATCACCGCCGAATACCGCCAACGGCTGGCCGCCGAAGGCAACCCGTGCAAGCTGATCTTCGTCACCCCCGAC 
TACTACGAGGAACGGCCGAAGGCCTGCATGGGCGGCTGGGCCAGCGTATTCCTCGACATCACCCCCGACGGC 
ACCGCGCTGCCGTGTCACAGCGCGCGGCAATTGCCGGTGCAGTTTCCCAACGTGCGCGAGCACAGCCTGCGC 
CACATCTGGTACGAATCGTTCGGCTTCAACCGTTACCGCGGCGACGCCTGGATGCCCGAACCCTGCCGTTCC 
TGCGAGGAGAAGGAGAGGGACCACGGCGGCTGCCGCTGCCAGGCGTTCCTCCTCACCGGCGATGCCGACGCC 
ACCGACCCGGTCTGCGCCAAGTCGGCACGGCATGACCTGATCCTCGCGGCCCGTCGCCAGGCCGAGGAGGCG 
CCGCTTGGCCTGGACGCGCTGACCTGGCGCAACCAGCGCGCGTCACGCCTGATCTGCAAGGCCTGA